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Band Related Discussions
  1. Western-No bands for sample protein but loading control works - (reply: 5)
  2. Weak band in negative control in PCR - (reply: 5)
  3. Faint band and primer dimer in PCR - (reply: 1)
  4. Problem with RNA bands on RNA bleach gel - (reply: 1)
  5. Anti-Penta His antibody detecting extra bands - (reply: 2)
  6. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
  7. High molecular weight band from my PCR - (reply: 3)
  8. GST fusion protein - two dominant bands - (reply: 7)
  9. bands becoming wavy with colour of the dye changing to yellow - (reply: 1)
  10. 2 bands very close in the expected place - (reply: 2)
  11. SDS-PAGE High MW bands dissapear! - (reply: 7)
  12. PCR bands on gel - (reply: 5)
  13. Electrophoresis problem: Frowning bands and smeary ladder - (reply: 1)
  14. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
  15. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
  16. No band detected in WB - (reply: 4)
  17. Western Blot diffuse bands - (reply: 3)
  18. Some bands transferred, some not..? Why??? - (reply: 1)
  19. Extraction of bands from SDS-PAGE - (reply: 2)
  20. Dumbbell-shaped bands on the WB - (reply: 3)
  21. Deformed bands in gel - (reply: 8)
  22. no bands after pcr!!!! - (reply: 4)
  23. Help in western blotting GAPDH bands are not even - (reply: 1)
  24. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
  25. detection of a protein band of protein complex - (reply: 3)
  26. How to determine gene knock-out from Agarose gel bands - (reply: 1)
  27. Two bands in WB but one band in IP - (reply: 1)
  28. Two bands in WB but one band in IP - (reply: 2)
  29. White bands in western blotting - (reply: 4)
  30. Colour of PCR gel band best for ImageJ analysis - (reply: 2)
  31. colony pcr, two strong bands - (reply: 4)
  32. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
  33. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
  34. PCR DNA bands too large on agarose gel - (reply: 1)
  35. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
  36. All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
  37. RNA gel problem (melted bands) - (reply: 2)
  38. The slightly bigger band dillema. - (reply: 2)
  39. Non especific band in PCR... - (reply: 2)
  40. Smeary bands in gel electrophoresis - (reply: 3)
  41. band obove the ladder - (reply: 3)
  42. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  43. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 2)
  44. unexpected protein bands - (reply: 2)
  45. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
  46. Band did not migrate on gel electrophoresis (SDS) - (reply: 3)
  47. RNA Gel 3 Sharp bands? - (reply: 1)
  48. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  49. Gel Bands - (reply: 1)
  50. No bands can be seen in my co-IP - (reply: 3)
  51. large bands in all pcr reactions including negative control - (reply: 1)
  52. reason for curved bands? - (reply: 2)
  53. Protein bands visible on unstained SDS-PAGE - (reply: 1)
  54. Digestion bands appear faint - (reply: 3)
  55. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
  56. double band with anti-Flag but not with anti-HA antibody - (reply: 1)
  57. Why Iím not getting any band after column purification? - (reply: 1)
  58. Strange band pattern in Western Blot - (reply: 1)
  59. Western Blot: protein band did not appeared at the correct location - (reply: 3)
  60. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
  61. Mysterious disappearing bands after blotting - (reply: 5)
  62. Western blot with smeared bands - (reply: 3)
  63. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  64. Thick 100-bp band disappeared under UV - (reply: 3)
  65. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  66. Electrophoresis after PCR : too many bands - (reply: 6)
  67. Nanog double band in WB - (reply: 1)
  68. My beta actin bands are changing - (reply: 6)
  69. Why does my gel have streaks instead of bands? - (reply: 2)
  70. Band on Western Blot are smeared - (reply: 1)
  71. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  72. Need help to identify bands in AGE result - (reply: 3)
  73. EMSA Shifted band in Free probe only lane - (reply: 1)
  74. Please help uneven bands in western blot - (reply: 9)
  75. Western Blot Problems...too many unspecific bands - (reply: 4)
  76. I never see the 16kDa band from the seeblue marker? - (reply: 3)
  77. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)
  78. Bands near edges running faster - (reply: 4)
  79. faint dna band after pcr purification - (reply: 1)
  80. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  81. Smeary bands on agarose gel (also the ladder) - (reply: 2)
  82. No Band With Large DNA (BAC) - (reply: 1)
  83. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
  84. i couldn't get the band of target gene after ligation - (reply: 9)
  85. IP: specific bands in control lanes - (reply: 1)
  86. Multiple Bands after gel extraction - (reply: 4)
  87. DNA extraction problem - strange bands - (reply: 4)
  88. Help!! Double band in genomic dna - (reply: 1)
  89. No bands despite different primers and conditions and TAqs - (reply: 8)
  90. unwanted band - (reply: 5)
  91. Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
  92. strong 100kda bands for alpha tubulin + the usual 55 kda bands? URGENT - (reply: 3)
  93. How to get rid of bands in PCR negative control - (reply: 10)
  94. T7 and M13 primers two band amplification - (reply: 3)
  95. Disappeared bands after the addition of Sigma P2714 - (reply: 1)
  96. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  97. Prey protein band bleeds, IgG band normal? - (reply: 3)
  98. negative control well 300 bp band - (reply: 3)
  99. How to confirm the band of interest in Westerns blot - (reply: 6)
  100. why i get double band for recombinant protein - (reply: 9)
  101. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  102. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
  103. Distorted bands from human colon samples - (reply: 2)
  104. "Wavy" bands in SDS-PAGE gel - (reply: 5)
  105. I get band double the size what does it mean - (reply: 3)
  106. Smeared Bands after Ponceau Staining? - (reply: 3)
  107. RFLP problem - missing band - (reply: 2)
  108. patchy/holey bands after transfer, invasive bubbles? - (reply: 7)
  109. fused bands of beta-actin in western blot - (reply: 2)
  110. how to get specific bands in co-ip - (reply: 5)
  111. faint band after plasmid extraction - (reply: 7)
  112. Undefined bands in Gel - (reply: 9)
  113. are they non-specific band? - (reply: 4)
  114. Trouble with purified recombinant protein bands - (reply: 3)
  115. 3 extra bands show up on my western after an IP - (reply: 2)
  116. Agarose gel bands gone (for gel extraction) - (reply: 2)
  117. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  118. 3' RACE creates too big band doesn't leave well - (reply: 1)
  119. RT-PCR product- no band - (reply: 4)
  120. Wierd Bands after PCR....Confused - (reply: 9)
  121. Quantify DNA in gel electrophoresis bands - (reply: 7)
  122. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
  123. Restriction digest band shift? - (reply: 1)
  124. No bands/weird bands on western blot - (reply: 3)
  125. Straight bands on DNA gel - (reply: 3)
  126. Western blot- bands weak - (reply: 10)
  127. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
  128. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  129. Curved bands in Western Blot - (reply: 2)
  130. Some bands not coming up, others uneven - (reply: 9)
  131. No bands in PCR after DNase treatment - (reply: 4)
  132. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
  133. B- actin band looks like dumbbells! - (reply: 2)
  134. Which antibody is good for HA-tag with low background bands? - (reply: 1)
  135. inespecific bands in nuclear fraction? - (reply: 1)
  136. PCR band slightly BELOW expected length ?! - (reply: 14)
  137. unexpected band in PCR with plasmid - (reply: 4)
  138. cloning: band at different site than desired after RE digestion - (reply: 11)
  139. bands position in agarose gel - (reply: 4)
  140. Multiple Bands in qPCR - (reply: 4)
  141. SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
  142. White bands on Cy3 gels - (reply: 1)
  143. Western Blot, Bax, Many bands - (reply: 3)
  144. Odd Bands on Gel - (reply: 3)
  145. qPCR: band in gel, but super high Cq? - (reply: 10)
  146. SDS Page and WB: Wrong size bands - (reply: 2)
  147. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
  148. Please help with odd bands and smears on Western blot - (reply: 4)
  149. Shift in the position of band onto the western blot - (reply: 3)
  150. Ghost and inverse bands and high background. Help! - (reply: 3)
  151. No resolving of protein/bands below 50kDa :-s - (reply: 5)
  152. "Mickey Mouse" bands - (reply: 5)
  153. aditional band on WB - (reply: 1)
  154. Bands dark on sides and light in middle - (reply: 7)
  155. nonspecific band at 50bp in PCR - (reply: 9)
  156. Protein bands not migrating - (reply: 3)
  157. Single Plasmid Gel Band - (reply: 1)
  158. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
  159. Detecting doublet bands in Western for eGFP control - (reply: 1)
  160. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  161. Uneven bands denaturing agarose - (reply: 2)
  162. two bands on WB-alternative use of start codons? - (reply: 2)
  163. PCR band moves lower than its size on agarose gel - (reply: 3)
  164. No WB bands for H2AX at all...! - (reply: 10)
  165. Overexpressed protein shows a band higher than expected?! - (reply: 2)
  166. Help! No bands after BSP! - (reply: 3)
  167. Multiple bands on blot with one antibody - (reply: 1)
  168. Multiple protein bands after expression - (reply: 6)
  169. Bands in negative control PCR - (reply: 2)
  170. No bands at all after double digestion - (reply: 1)
  171. Non-specific bands and fuzzy bands - (reply: 1)
  172. Weird extra bands in EMSA - (reply: 1)
  173. Help for western blot wet transfer blurry bands - (reply: 2)
  174. Blurry bands ... - (reply: 12)
  175. Strange Bands - (reply: 2)
  176. smearing below band of interest - (reply: 5)
  177. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  178. No or very weak band using 16s primers - (reply: 1)
  179. band shift after IP - (reply: 1)
  180. Western blot narrow bands - (reply: 1)
  181. Bizarre changes in plasmid band size - (reply: 3)
  182. Western blot band anaylisis - (reply: 3)
  183. RNA degradation problem, only one band on the gel - (reply: 3)
  184. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  185. 16s double bands obtained-why?? - (reply: 2)
  186. Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
  187. got band in control positive but not in sample... - (reply: 2)
  188. Band is at the wrong size, but same for all antibodies - (reply: 6)
  189. Puzzeling western blot results, missing band - (reply: 3)
  190. No bands appearing in DNA gel electrophoresis - (reply: 1)
  191. Multiple bands in eluted PCR product - (reply: 1)
  192. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  193. BAC miniprep-high nanodrop value but NO BAND - (reply: 2)
  194. multiple bands for genomic DNA extraction - (reply: 3)
  195. The same strong bands with different primary antibody - (reply: 1)
  196. many bands Western Blot - (reply: 2)
  197. unespecific band PCR from mouse genomic DNA - (reply: 9)
  198. Help with unknown band in restriction digest - (reply: 1)
  199. Preference for larger of 2 bands? - (reply: 2)
  200. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  201. RNase Band in old plasmid stored at -40 - (reply: 2)
  202. getting rid of non specific bands in IP experiments - (reply: 7)
  203. strange bands shape agarose gel - (reply: 2)
  204. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
  205. Getting too Many Bands on my Western Blot - (reply: 9)
  206. Extra bands after protein expression/purification - (reply: 4)
  207. sequencing result from excised DGGE bands - (reply: 3)
  208. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
  209. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  210. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  211. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  212. Analyzing Western Bands by Densiometry - (reply: 2)
  213. Problem: Cloning DNA shuffled band - (reply: 2)
  214. Problems with a PCR-No bands and I am starting to question my sanity - (reply: 4)
  215. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
  216. Problem with PCR - large extra bands - (reply: 3)
  217. Same non specific band for different target proteins - (reply: 1)
  218. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
  219. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
  220. No band in PCR - (reply: 5)
  221. Identically loaded western bands loose intensity from left to right - (reply: 3)
  222. smear bands in western blot - (reply: 12)
  223. bands fuse together - (reply: 1)
  224. Measuring of cDNA bands density - (reply: 3)
  225. No band after extracting DNA from bacillus subtilis - (reply: 1)
  226. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  227. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
  228. Re-using membranes with ghost bands - (reply: 2)
  229. huge dot appears at the centre of every of my bands - (reply: 2)
  230. Bright bands of PCR products stick in gel wells - (reply: 1)
  231. Separating close bands in Western Blot - (reply: 3)
  232. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  233. Bands in my PCR Controls - (reply: 3)
  234. Strange band in colony PCR - (reply: 2)
  235. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  236. Bands seen with qPCR missing in regular PCR - (reply: 5)
  237. non specific bands - (reply: 7)
  238. why cant get the band? - (reply: 4)
  239. Western Blot no target bands, show Non-specific bands - (reply: 5)
  240. Bands disappear after longer exposure - (reply: 4)
  241. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  242. How to remove the background and unspecific bands - (reply: 12)
  243. Same Non-specific bands show up for different Abs!! - (reply: 7)
  244. what caused bubble band of my WB? - (reply: 4)
  245. Strange bands and large smear in western blot - (reply: 4)
  246. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
  247. HELP!- strange band appearing on immunoblot - strange band detected during blotting with anti-CTB antibodies (reply: 3)
  248. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  249. Band Quantitation - Bio-Rad's Quantity One (reply: 1)
  250. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
  251. how to get rid of non specific bands in PCR - (reply: 1)
  252. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
  253. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
  254. SDS PAGE smearing of bands - (reply: 2)
  255. Fuzzy bands in western blot - (reply: 2)
  256. Large Non-specfic bands in PCR - (reply: 3)
  257. HELP!! I ruin my Native-PAGE (newbie in PAGE) - My bands seem faint smear (reply: 3)
  258. 2 bands in blue colony PCR - (reply: 5)
  259. no bands after pcr - even after we have change most of the reagents (reply: 2)
  260. Identify ponceau visible bands - (reply: 1)
  261. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  262. Many unspecific bands after using anti-His on Western blot - (reply: 4)
  263. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  264. KO mice show band for knocked-out protein - (reply: 1)
  265. western blot bands wider at the middle than the top - (reply: 2)
  266. PCR Gel nonspecific band - (reply: 13)
  267. can't get bands in Western Blot - (reply: 5)
  268. difference in band sizes ! - why ??? (reply: 2)
  269. Funny DNA bands on Agarose gels - (reply: 6)
  270. Actin Bands smiling on western blot - (reply: 3)
  271. PCR: always got band in the negative control - (reply: 8)
  272. nonspecific bands on film - (reply: 3)
  273. 'lace-like texture' on my northern blot bands - (reply: 8)
  274. Problem Viewing bands in Agarose Gel - (reply: 6)
  275. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  276. software for quantifing PCR product band - (reply: 1)
  277. Multiple bands from purified PCR product - (reply: 3)
  278. Quantification of proteins in Western Bands - (reply: 2)
  279. No bands on agarose gel after restriction digestion. - (reply: 10)
  280. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  281. Extra band on gel after miniprep - (reply: 6)
  282. 3 distinct bands of gDNA on gel - (reply: 2)
  283. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  284. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  285. Total RNA only produce 1 band in gel - (reply: 2)
  286. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
  287. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
  288. weird bands after minipreps - molecular cloning (reply: 2)
  289. Band at same position using different primary antibodies - (reply: 3)
  290. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  291. PCR, using gel extracted band as a template - (reply: 4)
  292. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  293. PCR bands in NTC but NOT in negative samples - (reply: 2)
  294. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
  295. Unusual band in transformants - (reply: 2)
  296. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  297. Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
  298. no band, extremely clean blot - (reply: 1)
  299. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  300. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  301. IgG - unspecific band (reply: 3)
  302. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
  303. "Unzipped" PCR band - (reply: 3)
  304. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  305. uncut vector contamination in gel purified band - (reply: 2)
  306. I see bands with western blot only the third day! - (reply: 2)
  307. incorrect/smaller band size for real time PCR - (reply: 1)
  308. Agarose gel bands smear/ drag problem - (reply: 4)
  309. Northern - Do not get Bands in Northern Blot (reply: 2)
  310. two bands detected in western - (reply: 1)
  311. Protein SDS-PAGE thick bands / smear - (reply: 4)
  312. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  313. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
  314. need help plz, band sizes estimation - (reply: 2)
  315. What causes this offset band on RIPA gel? - (reply: 4)
  316. nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
  317. band diffusion in sds - (reply: 5)
  318. too much bands in western - (reply: 2)
  319. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  320. Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
  321. Bands are lower than actual protein - (reply: 1)
  322. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  323. No band after WB revelation - (reply: 2)
  324. No band after digestion - (reply: 4)
  325. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
  326. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  327. PCR -No band formation - (reply: 1)
  328. PCR - No Band formation - (reply: 3)
  329. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
  330. 2d page - no band (reply: 2)
  331. never get bands by pfu related enzymes - (reply: 2)
  332. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  333. Lower MW bands than expected. - (reply: 1)
  334. Multiple bands after bisulfite PCR - (reply: 7)
  335. western bands look funny - (reply: 5)
  336. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  337. quantifying "ghost" bands - (reply: 1)
  338. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
  339. Extra bands after QIAGEN spin miniprep - (reply: 3)
  340. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  341. Cold probe doesnt compete away bands - (reply: 2)
  342. Strength of actin signal - How dark should the band be? (reply: 5)
  343. Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
  344. PCR product appear two close band in my gel - (reply: 15)
  345. The Case of a Missing Band: PCR Issue - (reply: 4)
  346. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  347. Band appear and disapper in the negative control ?? - (reply: 2)
  348. Bands in PCR negative control - (reply: 3)
  349. Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
  350. western exposure one part of band darker - (reply: 6)
  351. non-specific bands - (reply: 5)
  352. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  353. bands of protein seems like it did not move - (reply: 2)
  354. downshifted band - (reply: 4)
  355. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
  356. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
  357. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  358. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  359. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
  360. more than one band - (reply: 1)
  361. IGg bands on SDS PAGE - (reply: 2)
  362. PCR band too thick - PCR troubleshooting question (reply: 5)
  363. ugly merged bands in western blot - (reply: 3)
  364. PCR bands on gel electrophoresis - (reply: 5)
  365. Identify the band in western blot - (reply: 5)
  366. Ponceau staining - Appearence of bands (reply: 1)
  367. Multiple bands after amping insert - (reply: 2)
  368. Protein bands on the bottom of the gel - (reply: 5)
  369. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
  370. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
  371. Bands in a miRNA gel - (reply: 1)
  372. Gel Electrophoresis band problems - (reply: 3)
  373. Problem with protein samples - No bands detected at all on gel (reply: 2)
  374. Unhappy bands - (reply: 3)
  375. High MW PCR band seen...help needed - (reply: 7)
  376. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
  377. HELP! Genomic DNA, RNA-size bands - (reply: 2)
  378. No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
  379. "Diffuesed" bands of higher molecular weight - (reply: 6)
  380. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  381. strange band in coomassie blue staining - (reply: 3)
  382. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  383. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  384. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  385. Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
  386. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  387. Wrong bands on the blot - (reply: 4)
  388. Problems detecting my band! - (reply: 2)
  389. Western bands fading out, pleeeese help - (reply: 1)
  390. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
  391. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
  392. Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
  393. Bands visible in membrane but not in the film - ?? (reply: 10)
  394. non desired brigther bands - (reply: 7)
  395. Bands on adjescent lanes!!! - wierd!!! (reply: 11)
  396. Only DNA ladder , No desired band in PCR - (reply: 4)
  397. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  398. Lost a Band by changing thermocycler - (reply: 9)
  399. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
  400. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  401. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  402. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  403. Bands appearing out of no where?! - (reply: 5)
  404. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  405. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
  406. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  407. casting agarose gel troubleshooting - smeared bands (reply: 8)
  408. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  409. Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
  410. the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
  411. overloading GAPDH but nice band of interest - (reply: 2)
  412. Quantiying bands. - Determination of protein contens (reply: 2)
  413. after re-isolation of vector, get single band - (reply: 4)
  414. BSP PCR for help - no bands of tissue amplification (reply: 3)
  415. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  416. Too many bands in my SDS-PAGE - (reply: 5)
  417. Uneven bands in SDS-PAGE - (reply: 5)
  418. weak bands for methylated DNA - (reply: 4)
  419. Two bands for p53 on Western Blot - (reply: 7)
  420. nonspecific bands after co-ip - (reply: 2)
  421. No PCR bands with some templates - (reply: 5)
  422. EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
  423. Blurred low molecular weight bands in SDS-PAGE - Need your help (reply: 1)
  424. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
  425. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
  426. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
  427. light bands, dark smear, dark dimers - (reply: 2)
  428. pcr doesnt show any band for DNa walking - (reply: 8)
  429. Immunoprecipitation - Bands in negative control - (reply: 2)
  430. crappy bands after developing - (reply: 3)
  431. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  432. no bands in my PCR - (reply: 9)
  433. All gradient samples give bands! How come? - (reply: 4)
  434. many bands of smaller size than expected - (reply: 3)
  435. wrong bands after T-vector cloning - (reply: 3)
  436. abnormal band in agarose gel - Urgent help required (reply: 3)
  437. Ghost bands in western blot - Western blotting problems (reply: 9)
  438. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  439. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  440. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
  441. Non-specific bands on EMSA - (reply: 2)
  442. Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
  443. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  444. PCR ghost bands - (reply: 1)
  445. Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
  446. strange extra bands on digest of miniprep? - (reply: 5)
  447. GST preotin non specific preotins - Non specific GST bands (reply: 2)
  448. 3'RACE and the polyA tail - why only one band? (reply: 2)
  449. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
  450. A single band on WB smaller than expected - (reply: 3)
  451. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  452. Co-IP problem - Weird IgG band (reply: 1)
  453. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
  454. EMSA supershift - increased band intensity? - (reply: 1)
  455. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  456. no band in gel - gel electrophoresis (reply: 1)
  457. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
  458. Strange bands in western of RPE-1 cells - (reply: 1)
  459. no 18S band on RNA agarose gel - (reply: 1)
  460. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  461. multiple bands in my RNA sample? - (reply: 2)
  462. multiple bands in my RNA sample? - (reply: 2)
  463. Ghost band in SDS PAGE - (reply: 2)
  464. No band in the gel after ligation - (reply: 6)
  465. Unidentified DNA bands - (reply: 1)
  466. silica purification - problem with double bands after silica purification (reply: 1)
  467. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  468. normal PCR - two bands is found in one sample (reply: 3)
  469. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  470. After double digestion, one weird band on the very top! - (reply: 4)
  471. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  472. Immunoprecipitation (optimization?) problem - anti-ovalbumin lane has 225 kDa band. (reply: 7)
  473. strange double band in electrophoretic pattern - (reply: 2)
  474. protein band size determination - any software available? (reply: 2)