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Band Related Discussions
  1. Extraction of bands from SDS-PAGE - (reply: 2)
  2. Dumbbell-shaped bands on the WB - (reply: 1)
  3. Deformed bands in gel - (reply: 8)
  4. no bands after pcr!!!! - (reply: 4)
  5. Help in western blotting GAPDH bands are not even - (reply: 1)
  6. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
  7. detection of a protein band of protein complex - (reply: 3)
  8. How to determine gene knock-out from Agarose gel bands - (reply: 1)
  9. Two bands in WB but one band in IP - (reply: 1)
  10. Two bands in WB but one band in IP - (reply: 2)
  11. White bands in western blotting - (reply: 4)
  12. Colour of PCR gel band best for ImageJ analysis - (reply: 2)
  13. colony pcr, two strong bands - (reply: 4)
  14. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
  15. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
  16. PCR DNA bands too large on agarose gel - (reply: 1)
  17. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
  18. All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
  19. RNA gel problem (melted bands) - (reply: 2)
  20. The slightly bigger band dillema. - (reply: 2)
  21. Non especific band in PCR... - (reply: 2)
  22. Smeary bands in gel electrophoresis - (reply: 3)
  23. band obove the ladder - (reply: 3)
  24. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  25. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 1)
  26. unexpected protein bands - (reply: 2)
  27. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
  28. Band did not migrate on gel electrophoresis (SDS) - (reply: 3)
  29. RNA Gel 3 Sharp bands? - (reply: 1)
  30. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  31. Gel Bands - (reply: 1)
  32. No bands can be seen in my co-IP - (reply: 3)
  33. large bands in all pcr reactions including negative control - (reply: 1)
  34. reason for curved bands? - (reply: 2)
  35. Protein bands visible on unstained SDS-PAGE - (reply: 1)
  36. Digestion bands appear faint - (reply: 3)
  37. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
  38. double band with anti-Flag but not with anti-HA antibody - (reply: 1)
  39. Why Iím not getting any band after column purification? - (reply: 1)
  40. Strange band pattern in Western Blot - (reply: 1)
  41. Western Blot: protein band did not appeared at the correct location - (reply: 3)
  42. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
  43. Mysterious disappearing bands after blotting - (reply: 5)
  44. Western blot with smeared bands - (reply: 3)
  45. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  46. Thick 100-bp band disappeared under UV - (reply: 3)
  47. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  48. Electrophoresis after PCR : too many bands - (reply: 6)
  49. Nanog double band in WB - (reply: 1)
  50. My beta actin bands are changing - (reply: 6)
  51. Why does my gel have streaks instead of bands? - (reply: 2)
  52. Band on Western Blot are smeared - (reply: 1)
  53. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  54. Need help to identify bands in AGE result - (reply: 3)
  55. EMSA Shifted band in Free probe only lane - (reply: 1)
  56. Please help uneven bands in western blot - (reply: 9)
  57. Western Blot Problems...too many unspecific bands - (reply: 4)
  58. I never see the 16kDa band from the seeblue marker? - (reply: 3)
  59. Bands near edges running faster - (reply: 4)
  60. faint dna band after pcr purification - (reply: 1)
  61. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  62. Smeary bands on agarose gel (also the ladder) - (reply: 2)
  63. No Band With Large DNA (BAC) - (reply: 1)
  64. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
  65. i couldn't get the band of target gene after ligation - (reply: 9)
  66. IP: specific bands in control lanes - (reply: 1)
  67. Multiple Bands after gel extraction - (reply: 4)
  68. DNA extraction problem - strange bands - (reply: 4)
  69. Help!! Double band in genomic dna - (reply: 1)
  70. No bands despite different primers and conditions and TAqs - (reply: 8)
  71. unwanted band - (reply: 5)
  72. Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
  73. strong 100kda bands for alpha tubulin + the usual 55 kda bands? URGENT - (reply: 3)
  74. How to get rid of bands in PCR negative control - (reply: 10)
  75. T7 and M13 primers two band amplification - (reply: 3)
  76. Disappeared bands after the addition of Sigma P2714 - (reply: 1)
  77. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  78. Prey protein band bleeds, IgG band normal? - (reply: 3)
  79. negative control well 300 bp band - (reply: 3)
  80. How to confirm the band of interest in Westerns blot - (reply: 6)
  81. why i get double band for recombinant protein - (reply: 9)
  82. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  83. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
  84. Distorted bands from human colon samples - (reply: 2)
  85. "Wavy" bands in SDS-PAGE gel - (reply: 5)
  86. I get band double the size what does it mean - (reply: 3)
  87. Smeared Bands after Ponceau Staining? - (reply: 3)
  88. RFLP problem - missing band - (reply: 2)
  89. patchy/holey bands after transfer, invasive bubbles? - (reply: 5)
  90. fused bands of beta-actin in western blot - (reply: 2)
  91. how to get specific bands in co-ip - (reply: 5)
  92. faint band after plasmid extraction - (reply: 7)
  93. Undefined bands in Gel - (reply: 9)
  94. are they non-specific band? - (reply: 4)
  95. Trouble with purified recombinant protein bands - (reply: 3)
  96. 3 extra bands show up on my western after an IP - (reply: 2)
  97. Agarose gel bands gone (for gel extraction) - (reply: 2)
  98. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  99. 3' RACE creates too big band doesn't leave well - (reply: 1)
  100. RT-PCR product- no band - (reply: 4)
  101. Wierd Bands after PCR....Confused - (reply: 9)
  102. Quantify DNA in gel electrophoresis bands - (reply: 7)
  103. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
  104. Restriction digest band shift? - (reply: 1)
  105. No bands/weird bands on western blot - (reply: 3)
  106. Straight bands on DNA gel - (reply: 3)
  107. Western blot- bands weak - (reply: 10)
  108. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
  109. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  110. Curved bands in Western Blot - (reply: 2)
  111. Some bands not coming up, others uneven - (reply: 9)
  112. No bands in PCR after DNase treatment - (reply: 4)
  113. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
  114. B- actin band looks like dumbbells! - (reply: 2)
  115. Which antibody is good for HA-tag with low background bands? - (reply: 1)
  116. inespecific bands in nuclear fraction? - (reply: 1)
  117. PCR band slightly BELOW expected length ?! - (reply: 14)
  118. unexpected band in PCR with plasmid - (reply: 4)
  119. cloning: band at different site than desired after RE digestion - (reply: 11)
  120. bands position in agarose gel - (reply: 4)
  121. Multiple Bands in qPCR - (reply: 4)
  122. SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
  123. White bands on Cy3 gels - (reply: 1)
  124. Western Blot, Bax, Many bands - (reply: 3)
  125. Odd Bands on Gel - (reply: 3)
  126. qPCR: band in gel, but super high Cq? - (reply: 10)
  127. SDS Page and WB: Wrong size bands - (reply: 2)
  128. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
  129. Please help with odd bands and smears on Western blot - (reply: 4)
  130. Shift in the position of band onto the western blot - (reply: 3)
  131. Ghost and inverse bands and high background. Help! - (reply: 3)
  132. No resolving of protein/bands below 50kDa :-s - (reply: 5)
  133. "Mickey Mouse" bands - (reply: 5)
  134. aditional band on WB - (reply: 1)
  135. Bands dark on sides and light in middle - (reply: 7)
  136. nonspecific band at 50bp in PCR - (reply: 9)
  137. Protein bands not migrating - (reply: 3)
  138. Single Plasmid Gel Band - (reply: 1)
  139. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
  140. Detecting doublet bands in Western for eGFP control - (reply: 1)
  141. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  142. Uneven bands denaturing agarose - (reply: 2)
  143. two bands on WB-alternative use of start codons? - (reply: 2)
  144. PCR band moves lower than its size on agarose gel - (reply: 3)
  145. No WB bands for H2AX at all...! - (reply: 10)
  146. Overexpressed protein shows a band higher than expected?! - (reply: 2)
  147. Help! No bands after BSP! - (reply: 3)
  148. Multiple bands on blot with one antibody - (reply: 1)
  149. Multiple protein bands after expression - (reply: 6)
  150. Bands in negative control PCR - (reply: 2)
  151. No bands at all after double digestion - (reply: 1)
  152. Non-specific bands and fuzzy bands - (reply: 1)
  153. Weird extra bands in EMSA - (reply: 1)
  154. Help for western blot wet transfer blurry bands - (reply: 2)
  155. Blurry bands ... - (reply: 12)
  156. Strange Bands - (reply: 2)
  157. smearing below band of interest - (reply: 5)
  158. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  159. No or very weak band using 16s primers - (reply: 1)
  160. band shift after IP - (reply: 1)
  161. Western blot narrow bands - (reply: 1)
  162. Bizarre changes in plasmid band size - (reply: 3)
  163. Western blot band anaylisis - (reply: 3)
  164. RNA degradation problem, only one band on the gel - (reply: 3)
  165. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  166. 16s double bands obtained-why?? - (reply: 2)
  167. Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
  168. got band in control positive but not in sample... - (reply: 2)
  169. Band is at the wrong size, but same for all antibodies - (reply: 6)
  170. Puzzeling western blot results, missing band - (reply: 3)
  171. No bands appearing in DNA gel electrophoresis - (reply: 1)
  172. Multiple bands in eluted PCR product - (reply: 1)
  173. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  174. BAC miniprep-high nanodrop value but NO BAND - (reply: 2)
  175. multiple bands for genomic DNA extraction - (reply: 3)
  176. The same strong bands with different primary antibody - (reply: 1)
  177. many bands Western Blot - (reply: 2)
  178. unespecific band PCR from mouse genomic DNA - (reply: 9)
  179. Help with unknown band in restriction digest - (reply: 1)
  180. Preference for larger of 2 bands? - (reply: 2)
  181. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  182. RNase Band in old plasmid stored at -40 - (reply: 2)
  183. getting rid of non specific bands in IP experiments - (reply: 7)
  184. strange bands shape agarose gel - (reply: 2)
  185. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
  186. Getting too Many Bands on my Western Blot - (reply: 9)
  187. Extra bands after protein expression/purification - (reply: 4)
  188. sequencing result from excised DGGE bands - (reply: 3)
  189. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
  190. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  191. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  192. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  193. Analyzing Western Bands by Densiometry - (reply: 2)
  194. Problem: Cloning DNA shuffled band - (reply: 2)
  195. Problems with a PCR-No bands and I am starting to question my sanity - (reply: 4)
  196. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
  197. Problem with PCR - large extra bands - (reply: 3)
  198. Same non specific band for different target proteins - (reply: 1)
  199. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
  200. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
  201. No band in PCR - (reply: 5)
  202. Identically loaded western bands loose intensity from left to right - (reply: 3)
  203. smear bands in western blot - (reply: 12)
  204. bands fuse together - (reply: 1)
  205. Measuring of cDNA bands density - (reply: 3)
  206. No band after extracting DNA from bacillus subtilis - (reply: 1)
  207. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  208. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
  209. Re-using membranes with ghost bands - (reply: 2)
  210. huge dot appears at the centre of every of my bands - (reply: 2)
  211. Bright bands of PCR products stick in gel wells - (reply: 1)
  212. Separating close bands in Western Blot - (reply: 3)
  213. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  214. Bands in my PCR Controls - (reply: 3)
  215. Strange band in colony PCR - (reply: 2)
  216. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  217. Bands seen with qPCR missing in regular PCR - (reply: 5)
  218. non specific bands - (reply: 7)
  219. why cant get the band? - (reply: 4)
  220. Western Blot no target bands, show Non-specific bands - (reply: 5)
  221. Bands disappear after longer exposure - (reply: 4)
  222. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  223. How to remove the background and unspecific bands - (reply: 12)
  224. Same Non-specific bands show up for different Abs!! - (reply: 7)
  225. what caused bubble band of my WB? - (reply: 4)
  226. Strange bands and large smear in western blot - (reply: 4)
  227. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
  228. HELP!- strange band appearing on immunoblot - strange band detected during blotting with anti-CTB antibodies (reply: 3)
  229. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  230. Band Quantitation - Bio-Rad's Quantity One (reply: 1)
  231. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
  232. how to get rid of non specific bands in PCR - (reply: 1)
  233. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
  234. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
  235. SDS PAGE smearing of bands - (reply: 2)
  236. Fuzzy bands in western blot - (reply: 2)
  237. Large Non-specfic bands in PCR - (reply: 3)
  238. HELP!! I ruin my Native-PAGE (newbie in PAGE) - My bands seem faint smear (reply: 3)
  239. 2 bands in blue colony PCR - (reply: 5)
  240. no bands after pcr - even after we have change most of the reagents (reply: 2)
  241. Identify ponceau visible bands - (reply: 1)
  242. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  243. Many unspecific bands after using anti-His on Western blot - (reply: 4)
  244. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  245. KO mice show band for knocked-out protein - (reply: 1)
  246. western blot bands wider at the middle than the top - (reply: 2)
  247. PCR Gel nonspecific band - (reply: 13)
  248. can't get bands in Western Blot - (reply: 5)
  249. difference in band sizes ! - why ??? (reply: 2)
  250. Funny DNA bands on Agarose gels - (reply: 6)
  251. Actin Bands smiling on western blot - (reply: 3)
  252. PCR: always got band in the negative control - (reply: 8)
  253. nonspecific bands on film - (reply: 3)
  254. 'lace-like texture' on my northern blot bands - (reply: 8)
  255. Problem Viewing bands in Agarose Gel - (reply: 6)
  256. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  257. software for quantifing PCR product band - (reply: 1)
  258. Multiple bands from purified PCR product - (reply: 3)
  259. Quantification of proteins in Western Bands - (reply: 2)
  260. No bands on agarose gel after restriction digestion. - (reply: 10)
  261. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  262. Extra band on gel after miniprep - (reply: 6)
  263. 3 distinct bands of gDNA on gel - (reply: 2)
  264. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  265. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  266. Total RNA only produce 1 band in gel - (reply: 2)
  267. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
  268. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
  269. weird bands after minipreps - molecular cloning (reply: 2)
  270. Band at same position using different primary antibodies - (reply: 3)
  271. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  272. PCR, using gel extracted band as a template - (reply: 4)
  273. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  274. PCR bands in NTC but NOT in negative samples - (reply: 2)
  275. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
  276. Unusual band in transformants - (reply: 2)
  277. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  278. Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
  279. no band, extremely clean blot - (reply: 1)
  280. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  281. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  282. IgG - unspecific band (reply: 3)
  283. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
  284. "Unzipped" PCR band - (reply: 3)
  285. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  286. uncut vector contamination in gel purified band - (reply: 2)
  287. I see bands with western blot only the third day! - (reply: 2)
  288. incorrect/smaller band size for real time PCR - (reply: 1)
  289. Agarose gel bands smear/ drag problem - (reply: 4)
  290. Northern - Do not get Bands in Northern Blot (reply: 2)
  291. two bands detected in western - (reply: 1)
  292. Protein SDS-PAGE thick bands / smear - (reply: 4)
  293. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  294. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
  295. need help plz, band sizes estimation - (reply: 2)
  296. What causes this offset band on RIPA gel? - (reply: 4)
  297. nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
  298. band diffusion in sds - (reply: 5)
  299. too much bands in western - (reply: 2)
  300. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  301. Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
  302. Bands are lower than actual protein - (reply: 1)
  303. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  304. No band after WB revelation - (reply: 2)
  305. No band after digestion - (reply: 4)
  306. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
  307. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  308. PCR -No band formation - (reply: 1)
  309. PCR - No Band formation - (reply: 3)
  310. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
  311. 2d page - no band (reply: 2)
  312. never get bands by pfu related enzymes - (reply: 2)
  313. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  314. Lower MW bands than expected. - (reply: 1)
  315. Multiple bands after bisulfite PCR - (reply: 7)
  316. western bands look funny - (reply: 5)
  317. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  318. quantifying "ghost" bands - (reply: 1)
  319. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
  320. Extra bands after QIAGEN spin miniprep - (reply: 3)
  321. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  322. Cold probe doesnt compete away bands - (reply: 2)
  323. Strength of actin signal - How dark should the band be? (reply: 5)
  324. Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
  325. PCR product appear two close band in my gel - (reply: 15)
  326. The Case of a Missing Band: PCR Issue - (reply: 4)
  327. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  328. Band appear and disapper in the negative control ?? - (reply: 2)
  329. Bands in PCR negative control - (reply: 3)
  330. Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
  331. western exposure one part of band darker - (reply: 6)
  332. non-specific bands - (reply: 5)
  333. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  334. bands of protein seems like it did not move - (reply: 2)
  335. downshifted band - (reply: 4)
  336. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
  337. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
  338. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  339. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  340. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
  341. more than one band - (reply: 1)
  342. IGg bands on SDS PAGE - (reply: 2)
  343. PCR band too thick - PCR troubleshooting question (reply: 5)
  344. ugly merged bands in western blot - (reply: 3)
  345. PCR bands on gel electrophoresis - (reply: 5)
  346. Identify the band in western blot - (reply: 5)
  347. Ponceau staining - Appearence of bands (reply: 1)
  348. Multiple bands after amping insert - (reply: 2)
  349. Protein bands on the bottom of the gel - (reply: 5)
  350. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
  351. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
  352. Bands in a miRNA gel - (reply: 1)
  353. Gel Electrophoresis band problems - (reply: 3)
  354. Problem with protein samples - No bands detected at all on gel (reply: 2)
  355. Unhappy bands - (reply: 3)
  356. High MW PCR band seen...help needed - (reply: 7)
  357. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
  358. HELP! Genomic DNA, RNA-size bands - (reply: 2)
  359. No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
  360. "Diffuesed" bands of higher molecular weight - (reply: 6)
  361. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  362. strange band in coomassie blue staining - (reply: 3)
  363. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  364. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  365. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  366. Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
  367. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  368. Wrong bands on the blot - (reply: 4)
  369. Problems detecting my band! - (reply: 2)
  370. Western bands fading out, pleeeese help - (reply: 1)
  371. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
  372. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
  373. Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
  374. Bands visible in membrane but not in the film - ?? (reply: 10)
  375. non desired brigther bands - (reply: 7)
  376. Bands on adjescent lanes!!! - wierd!!! (reply: 11)
  377. Only DNA ladder , No desired band in PCR - (reply: 4)
  378. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  379. Lost a Band by changing thermocycler - (reply: 9)
  380. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
  381. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  382. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  383. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  384. Bands appearing out of no where?! - (reply: 5)
  385. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  386. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
  387. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  388. casting agarose gel troubleshooting - smeared bands (reply: 8)
  389. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  390. Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
  391. the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
  392. overloading GAPDH but nice band of interest - (reply: 2)
  393. Quantiying bands. - Determination of protein contens (reply: 2)
  394. after re-isolation of vector, get single band - (reply: 4)
  395. BSP PCR for help - no bands of tissue amplification (reply: 3)
  396. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  397. Too many bands in my SDS-PAGE - (reply: 5)
  398. Uneven bands in SDS-PAGE - (reply: 5)
  399. weak bands for methylated DNA - (reply: 4)
  400. Two bands for p53 on Western Blot - (reply: 7)
  401. nonspecific bands after co-ip - (reply: 2)
  402. No PCR bands with some templates - (reply: 5)
  403. EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
  404. Blurred low molecular weight bands in SDS-PAGE - Need your help (reply: 1)
  405. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
  406. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
  407. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
  408. light bands, dark smear, dark dimers - (reply: 2)
  409. pcr doesnt show any band for DNa walking - (reply: 8)
  410. Immunoprecipitation - Bands in negative control - (reply: 2)
  411. crappy bands after developing - (reply: 3)
  412. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  413. no bands in my PCR - (reply: 9)
  414. All gradient samples give bands! How come? - (reply: 4)
  415. many bands of smaller size than expected - (reply: 3)
  416. wrong bands after T-vector cloning - (reply: 3)
  417. abnormal band in agarose gel - Urgent help required (reply: 3)
  418. Ghost bands in western blot - Western blotting problems (reply: 9)
  419. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  420. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  421. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
  422. Non-specific bands on EMSA - (reply: 2)
  423. Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
  424. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  425. PCR ghost bands - (reply: 1)
  426. Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
  427. strange extra bands on digest of miniprep? - (reply: 5)
  428. GST preotin non specific preotins - Non specific GST bands (reply: 2)
  429. 3'RACE and the polyA tail - why only one band? (reply: 2)
  430. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
  431. A single band on WB smaller than expected - (reply: 3)
  432. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  433. Co-IP problem - Weird IgG band (reply: 1)
  434. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
  435. EMSA supershift - increased band intensity? - (reply: 1)
  436. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  437. no band in gel - gel electrophoresis (reply: 1)
  438. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
  439. Strange bands in western of RPE-1 cells - (reply: 1)
  440. no 18S band on RNA agarose gel - (reply: 1)
  441. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  442. multiple bands in my RNA sample? - (reply: 2)
  443. multiple bands in my RNA sample? - (reply: 2)
  444. Ghost band in SDS PAGE - (reply: 2)
  445. No band in the gel after ligation - (reply: 6)
  446. Unidentified DNA bands - (reply: 1)
  447. silica purification - problem with double bands after silica purification (reply: 1)
  448. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  449. normal PCR - two bands is found in one sample (reply: 3)
  450. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  451. After double digestion, one weird band on the very top! - (reply: 4)
  452. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  453. Immunoprecipitation (optimization?) problem - anti-ovalbumin lane has 225 kDa band. (reply: 7)
  454. strange double band in electrophoretic pattern - (reply: 2)
  455. protein band size determination - any software available? (reply: 2)