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Band Related Discussions
  1. Anti-Penta His antibody detecting extra bands - (reply: 1)
  2. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
  3. High molecular weight band from my PCR - (reply: 3)
  4. GST fusion protein - two dominant bands - (reply: 3)
  5. bands becoming wavy with colour of the dye changing to yellow - (reply: 1)
  6. 2 bands very close in the expected place - (reply: 2)
  7. SDS-PAGE High MW bands dissapear! - (reply: 7)
  8. PCR bands on gel - (reply: 5)
  9. Electrophoresis problem: Frowning bands and smeary ladder - (reply: 1)
  10. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
  11. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
  12. No band detected in WB - (reply: 4)
  13. Western Blot diffuse bands - (reply: 3)
  14. Some bands transferred, some not..? Why??? - (reply: 1)
  15. Extraction of bands from SDS-PAGE - (reply: 2)
  16. Dumbbell-shaped bands on the WB - (reply: 3)
  17. Deformed bands in gel - (reply: 8)
  18. no bands after pcr!!!! - (reply: 4)
  19. Help in western blotting GAPDH bands are not even - (reply: 1)
  20. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
  21. detection of a protein band of protein complex - (reply: 3)
  22. How to determine gene knock-out from Agarose gel bands - (reply: 1)
  23. Two bands in WB but one band in IP - (reply: 1)
  24. Two bands in WB but one band in IP - (reply: 2)
  25. White bands in western blotting - (reply: 4)
  26. Colour of PCR gel band best for ImageJ analysis - (reply: 2)
  27. colony pcr, two strong bands - (reply: 4)
  28. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
  29. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
  30. PCR DNA bands too large on agarose gel - (reply: 1)
  31. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
  32. All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
  33. RNA gel problem (melted bands) - (reply: 2)
  34. The slightly bigger band dillema. - (reply: 2)
  35. Non especific band in PCR... - (reply: 2)
  36. Smeary bands in gel electrophoresis - (reply: 3)
  37. band obove the ladder - (reply: 3)
  38. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  39. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 2)
  40. unexpected protein bands - (reply: 2)
  41. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
  42. Band did not migrate on gel electrophoresis (SDS) - (reply: 3)
  43. RNA Gel 3 Sharp bands? - (reply: 1)
  44. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  45. Gel Bands - (reply: 1)
  46. No bands can be seen in my co-IP - (reply: 3)
  47. large bands in all pcr reactions including negative control - (reply: 1)
  48. reason for curved bands? - (reply: 2)
  49. Protein bands visible on unstained SDS-PAGE - (reply: 1)
  50. Digestion bands appear faint - (reply: 3)
  51. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
  52. double band with anti-Flag but not with anti-HA antibody - (reply: 1)
  53. Why Iím not getting any band after column purification? - (reply: 1)
  54. Strange band pattern in Western Blot - (reply: 1)
  55. Western Blot: protein band did not appeared at the correct location - (reply: 3)
  56. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
  57. Mysterious disappearing bands after blotting - (reply: 5)
  58. Western blot with smeared bands - (reply: 3)
  59. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  60. Thick 100-bp band disappeared under UV - (reply: 3)
  61. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  62. Electrophoresis after PCR : too many bands - (reply: 6)
  63. Nanog double band in WB - (reply: 1)
  64. My beta actin bands are changing - (reply: 6)
  65. Why does my gel have streaks instead of bands? - (reply: 2)
  66. Band on Western Blot are smeared - (reply: 1)
  67. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  68. Need help to identify bands in AGE result - (reply: 3)
  69. EMSA Shifted band in Free probe only lane - (reply: 1)
  70. Please help uneven bands in western blot - (reply: 9)
  71. Western Blot Problems...too many unspecific bands - (reply: 4)
  72. I never see the 16kDa band from the seeblue marker? - (reply: 3)
  73. Bands near edges running faster - (reply: 4)
  74. faint dna band after pcr purification - (reply: 1)
  75. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  76. Smeary bands on agarose gel (also the ladder) - (reply: 2)
  77. No Band With Large DNA (BAC) - (reply: 1)
  78. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
  79. i couldn't get the band of target gene after ligation - (reply: 9)
  80. IP: specific bands in control lanes - (reply: 1)
  81. Multiple Bands after gel extraction - (reply: 4)
  82. DNA extraction problem - strange bands - (reply: 4)
  83. Help!! Double band in genomic dna - (reply: 1)
  84. No bands despite different primers and conditions and TAqs - (reply: 8)
  85. unwanted band - (reply: 5)
  86. Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
  87. strong 100kda bands for alpha tubulin + the usual 55 kda bands? URGENT - (reply: 3)
  88. How to get rid of bands in PCR negative control - (reply: 10)
  89. T7 and M13 primers two band amplification - (reply: 3)
  90. Disappeared bands after the addition of Sigma P2714 - (reply: 1)
  91. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  92. Prey protein band bleeds, IgG band normal? - (reply: 3)
  93. negative control well 300 bp band - (reply: 3)
  94. How to confirm the band of interest in Westerns blot - (reply: 6)
  95. why i get double band for recombinant protein - (reply: 9)
  96. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  97. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
  98. Distorted bands from human colon samples - (reply: 2)
  99. "Wavy" bands in SDS-PAGE gel - (reply: 5)
  100. I get band double the size what does it mean - (reply: 3)
  101. Smeared Bands after Ponceau Staining? - (reply: 3)
  102. RFLP problem - missing band - (reply: 2)
  103. patchy/holey bands after transfer, invasive bubbles? - (reply: 7)
  104. fused bands of beta-actin in western blot - (reply: 2)
  105. how to get specific bands in co-ip - (reply: 5)
  106. faint band after plasmid extraction - (reply: 7)
  107. Undefined bands in Gel - (reply: 9)
  108. are they non-specific band? - (reply: 4)
  109. Trouble with purified recombinant protein bands - (reply: 3)
  110. 3 extra bands show up on my western after an IP - (reply: 2)
  111. Agarose gel bands gone (for gel extraction) - (reply: 2)
  112. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  113. 3' RACE creates too big band doesn't leave well - (reply: 1)
  114. RT-PCR product- no band - (reply: 4)
  115. Wierd Bands after PCR....Confused - (reply: 9)
  116. Quantify DNA in gel electrophoresis bands - (reply: 7)
  117. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
  118. Restriction digest band shift? - (reply: 1)
  119. No bands/weird bands on western blot - (reply: 3)
  120. Straight bands on DNA gel - (reply: 3)
  121. Western blot- bands weak - (reply: 10)
  122. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
  123. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  124. Curved bands in Western Blot - (reply: 2)
  125. Some bands not coming up, others uneven - (reply: 9)
  126. No bands in PCR after DNase treatment - (reply: 4)
  127. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
  128. B- actin band looks like dumbbells! - (reply: 2)
  129. Which antibody is good for HA-tag with low background bands? - (reply: 1)
  130. inespecific bands in nuclear fraction? - (reply: 1)
  131. PCR band slightly BELOW expected length ?! - (reply: 14)
  132. unexpected band in PCR with plasmid - (reply: 4)
  133. cloning: band at different site than desired after RE digestion - (reply: 11)
  134. bands position in agarose gel - (reply: 4)
  135. Multiple Bands in qPCR - (reply: 4)
  136. SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
  137. White bands on Cy3 gels - (reply: 1)
  138. Western Blot, Bax, Many bands - (reply: 3)
  139. Odd Bands on Gel - (reply: 3)
  140. qPCR: band in gel, but super high Cq? - (reply: 10)
  141. SDS Page and WB: Wrong size bands - (reply: 2)
  142. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
  143. Please help with odd bands and smears on Western blot - (reply: 4)
  144. Shift in the position of band onto the western blot - (reply: 3)
  145. Ghost and inverse bands and high background. Help! - (reply: 3)
  146. No resolving of protein/bands below 50kDa :-s - (reply: 5)
  147. "Mickey Mouse" bands - (reply: 5)
  148. aditional band on WB - (reply: 1)
  149. Bands dark on sides and light in middle - (reply: 7)
  150. nonspecific band at 50bp in PCR - (reply: 9)
  151. Protein bands not migrating - (reply: 3)
  152. Single Plasmid Gel Band - (reply: 1)
  153. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
  154. Detecting doublet bands in Western for eGFP control - (reply: 1)
  155. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  156. Uneven bands denaturing agarose - (reply: 2)
  157. two bands on WB-alternative use of start codons? - (reply: 2)
  158. PCR band moves lower than its size on agarose gel - (reply: 3)
  159. No WB bands for H2AX at all...! - (reply: 10)
  160. Overexpressed protein shows a band higher than expected?! - (reply: 2)
  161. Help! No bands after BSP! - (reply: 3)
  162. Multiple bands on blot with one antibody - (reply: 1)
  163. Multiple protein bands after expression - (reply: 6)
  164. Bands in negative control PCR - (reply: 2)
  165. No bands at all after double digestion - (reply: 1)
  166. Non-specific bands and fuzzy bands - (reply: 1)
  167. Weird extra bands in EMSA - (reply: 1)
  168. Help for western blot wet transfer blurry bands - (reply: 2)
  169. Blurry bands ... - (reply: 12)
  170. Strange Bands - (reply: 2)
  171. smearing below band of interest - (reply: 5)
  172. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  173. No or very weak band using 16s primers - (reply: 1)
  174. band shift after IP - (reply: 1)
  175. Western blot narrow bands - (reply: 1)
  176. Bizarre changes in plasmid band size - (reply: 3)
  177. Western blot band anaylisis - (reply: 3)
  178. RNA degradation problem, only one band on the gel - (reply: 3)
  179. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  180. 16s double bands obtained-why?? - (reply: 2)
  181. Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
  182. got band in control positive but not in sample... - (reply: 2)
  183. Band is at the wrong size, but same for all antibodies - (reply: 6)
  184. Puzzeling western blot results, missing band - (reply: 3)
  185. No bands appearing in DNA gel electrophoresis - (reply: 1)
  186. Multiple bands in eluted PCR product - (reply: 1)
  187. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  188. BAC miniprep-high nanodrop value but NO BAND - (reply: 2)
  189. multiple bands for genomic DNA extraction - (reply: 3)
  190. The same strong bands with different primary antibody - (reply: 1)
  191. many bands Western Blot - (reply: 2)
  192. unespecific band PCR from mouse genomic DNA - (reply: 9)
  193. Help with unknown band in restriction digest - (reply: 1)
  194. Preference for larger of 2 bands? - (reply: 2)
  195. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  196. RNase Band in old plasmid stored at -40 - (reply: 2)
  197. getting rid of non specific bands in IP experiments - (reply: 7)
  198. strange bands shape agarose gel - (reply: 2)
  199. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
  200. Getting too Many Bands on my Western Blot - (reply: 9)
  201. Extra bands after protein expression/purification - (reply: 4)
  202. sequencing result from excised DGGE bands - (reply: 3)
  203. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
  204. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  205. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  206. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  207. Analyzing Western Bands by Densiometry - (reply: 2)
  208. Problem: Cloning DNA shuffled band - (reply: 2)
  209. Problems with a PCR-No bands and I am starting to question my sanity - (reply: 4)
  210. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
  211. Problem with PCR - large extra bands - (reply: 3)
  212. Same non specific band for different target proteins - (reply: 1)
  213. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
  214. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
  215. No band in PCR - (reply: 5)
  216. Identically loaded western bands loose intensity from left to right - (reply: 3)
  217. smear bands in western blot - (reply: 12)
  218. bands fuse together - (reply: 1)
  219. Measuring of cDNA bands density - (reply: 3)
  220. No band after extracting DNA from bacillus subtilis - (reply: 1)
  221. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  222. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
  223. Re-using membranes with ghost bands - (reply: 2)
  224. huge dot appears at the centre of every of my bands - (reply: 2)
  225. Bright bands of PCR products stick in gel wells - (reply: 1)
  226. Separating close bands in Western Blot - (reply: 3)
  227. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  228. Bands in my PCR Controls - (reply: 3)
  229. Strange band in colony PCR - (reply: 2)
  230. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  231. Bands seen with qPCR missing in regular PCR - (reply: 5)
  232. non specific bands - (reply: 7)
  233. why cant get the band? - (reply: 4)
  234. Western Blot no target bands, show Non-specific bands - (reply: 5)
  235. Bands disappear after longer exposure - (reply: 4)
  236. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  237. How to remove the background and unspecific bands - (reply: 12)
  238. Same Non-specific bands show up for different Abs!! - (reply: 7)
  239. what caused bubble band of my WB? - (reply: 4)
  240. Strange bands and large smear in western blot - (reply: 4)
  241. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
  242. HELP!- strange band appearing on immunoblot - strange band detected during blotting with anti-CTB antibodies (reply: 3)
  243. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  244. Band Quantitation - Bio-Rad's Quantity One (reply: 1)
  245. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
  246. how to get rid of non specific bands in PCR - (reply: 1)
  247. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
  248. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
  249. SDS PAGE smearing of bands - (reply: 2)
  250. Fuzzy bands in western blot - (reply: 2)
  251. Large Non-specfic bands in PCR - (reply: 3)
  252. HELP!! I ruin my Native-PAGE (newbie in PAGE) - My bands seem faint smear (reply: 3)
  253. 2 bands in blue colony PCR - (reply: 5)
  254. no bands after pcr - even after we have change most of the reagents (reply: 2)
  255. Identify ponceau visible bands - (reply: 1)
  256. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  257. Many unspecific bands after using anti-His on Western blot - (reply: 4)
  258. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  259. KO mice show band for knocked-out protein - (reply: 1)
  260. western blot bands wider at the middle than the top - (reply: 2)
  261. PCR Gel nonspecific band - (reply: 13)
  262. can't get bands in Western Blot - (reply: 5)
  263. difference in band sizes ! - why ??? (reply: 2)
  264. Funny DNA bands on Agarose gels - (reply: 6)
  265. Actin Bands smiling on western blot - (reply: 3)
  266. PCR: always got band in the negative control - (reply: 8)
  267. nonspecific bands on film - (reply: 3)
  268. 'lace-like texture' on my northern blot bands - (reply: 8)
  269. Problem Viewing bands in Agarose Gel - (reply: 6)
  270. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  271. software for quantifing PCR product band - (reply: 1)
  272. Multiple bands from purified PCR product - (reply: 3)
  273. Quantification of proteins in Western Bands - (reply: 2)
  274. No bands on agarose gel after restriction digestion. - (reply: 10)
  275. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  276. Extra band on gel after miniprep - (reply: 6)
  277. 3 distinct bands of gDNA on gel - (reply: 2)
  278. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  279. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  280. Total RNA only produce 1 band in gel - (reply: 2)
  281. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
  282. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
  283. weird bands after minipreps - molecular cloning (reply: 2)
  284. Band at same position using different primary antibodies - (reply: 3)
  285. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  286. PCR, using gel extracted band as a template - (reply: 4)
  287. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  288. PCR bands in NTC but NOT in negative samples - (reply: 2)
  289. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
  290. Unusual band in transformants - (reply: 2)
  291. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  292. Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
  293. no band, extremely clean blot - (reply: 1)
  294. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  295. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  296. IgG - unspecific band (reply: 3)
  297. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
  298. "Unzipped" PCR band - (reply: 3)
  299. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  300. uncut vector contamination in gel purified band - (reply: 2)
  301. I see bands with western blot only the third day! - (reply: 2)
  302. incorrect/smaller band size for real time PCR - (reply: 1)
  303. Agarose gel bands smear/ drag problem - (reply: 4)
  304. Northern - Do not get Bands in Northern Blot (reply: 2)
  305. two bands detected in western - (reply: 1)
  306. Protein SDS-PAGE thick bands / smear - (reply: 4)
  307. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  308. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
  309. need help plz, band sizes estimation - (reply: 2)
  310. What causes this offset band on RIPA gel? - (reply: 4)
  311. nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
  312. band diffusion in sds - (reply: 5)
  313. too much bands in western - (reply: 2)
  314. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  315. Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
  316. Bands are lower than actual protein - (reply: 1)
  317. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  318. No band after WB revelation - (reply: 2)
  319. No band after digestion - (reply: 4)
  320. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
  321. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  322. PCR -No band formation - (reply: 1)
  323. PCR - No Band formation - (reply: 3)
  324. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
  325. 2d page - no band (reply: 2)
  326. never get bands by pfu related enzymes - (reply: 2)
  327. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  328. Lower MW bands than expected. - (reply: 1)
  329. Multiple bands after bisulfite PCR - (reply: 7)
  330. western bands look funny - (reply: 5)
  331. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  332. quantifying "ghost" bands - (reply: 1)
  333. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
  334. Extra bands after QIAGEN spin miniprep - (reply: 3)
  335. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  336. Cold probe doesnt compete away bands - (reply: 2)
  337. Strength of actin signal - How dark should the band be? (reply: 5)
  338. Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
  339. PCR product appear two close band in my gel - (reply: 15)
  340. The Case of a Missing Band: PCR Issue - (reply: 4)
  341. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  342. Band appear and disapper in the negative control ?? - (reply: 2)
  343. Bands in PCR negative control - (reply: 3)
  344. Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
  345. western exposure one part of band darker - (reply: 6)
  346. non-specific bands - (reply: 5)
  347. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  348. bands of protein seems like it did not move - (reply: 2)
  349. downshifted band - (reply: 4)
  350. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
  351. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
  352. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  353. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  354. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
  355. more than one band - (reply: 1)
  356. IGg bands on SDS PAGE - (reply: 2)
  357. PCR band too thick - PCR troubleshooting question (reply: 5)
  358. ugly merged bands in western blot - (reply: 3)
  359. PCR bands on gel electrophoresis - (reply: 5)
  360. Identify the band in western blot - (reply: 5)
  361. Ponceau staining - Appearence of bands (reply: 1)
  362. Multiple bands after amping insert - (reply: 2)
  363. Protein bands on the bottom of the gel - (reply: 5)
  364. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
  365. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
  366. Bands in a miRNA gel - (reply: 1)
  367. Gel Electrophoresis band problems - (reply: 3)
  368. Problem with protein samples - No bands detected at all on gel (reply: 2)
  369. Unhappy bands - (reply: 3)
  370. High MW PCR band seen...help needed - (reply: 7)
  371. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
  372. HELP! Genomic DNA, RNA-size bands - (reply: 2)
  373. No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
  374. "Diffuesed" bands of higher molecular weight - (reply: 6)
  375. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  376. strange band in coomassie blue staining - (reply: 3)
  377. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  378. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  379. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  380. Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
  381. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  382. Wrong bands on the blot - (reply: 4)
  383. Problems detecting my band! - (reply: 2)
  384. Western bands fading out, pleeeese help - (reply: 1)
  385. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
  386. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
  387. Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
  388. Bands visible in membrane but not in the film - ?? (reply: 10)
  389. non desired brigther bands - (reply: 7)
  390. Bands on adjescent lanes!!! - wierd!!! (reply: 11)
  391. Only DNA ladder , No desired band in PCR - (reply: 4)
  392. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  393. Lost a Band by changing thermocycler - (reply: 9)
  394. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
  395. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  396. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  397. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  398. Bands appearing out of no where?! - (reply: 5)
  399. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  400. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
  401. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  402. casting agarose gel troubleshooting - smeared bands (reply: 8)
  403. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  404. Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
  405. the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
  406. overloading GAPDH but nice band of interest - (reply: 2)
  407. Quantiying bands. - Determination of protein contens (reply: 2)
  408. after re-isolation of vector, get single band - (reply: 4)
  409. BSP PCR for help - no bands of tissue amplification (reply: 3)
  410. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  411. Too many bands in my SDS-PAGE - (reply: 5)
  412. Uneven bands in SDS-PAGE - (reply: 5)
  413. weak bands for methylated DNA - (reply: 4)
  414. Two bands for p53 on Western Blot - (reply: 7)
  415. nonspecific bands after co-ip - (reply: 2)
  416. No PCR bands with some templates - (reply: 5)
  417. EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
  418. Blurred low molecular weight bands in SDS-PAGE - Need your help (reply: 1)
  419. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
  420. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
  421. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
  422. light bands, dark smear, dark dimers - (reply: 2)
  423. pcr doesnt show any band for DNa walking - (reply: 8)
  424. Immunoprecipitation - Bands in negative control - (reply: 2)
  425. crappy bands after developing - (reply: 3)
  426. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  427. no bands in my PCR - (reply: 9)
  428. All gradient samples give bands! How come? - (reply: 4)
  429. many bands of smaller size than expected - (reply: 3)
  430. wrong bands after T-vector cloning - (reply: 3)
  431. abnormal band in agarose gel - Urgent help required (reply: 3)
  432. Ghost bands in western blot - Western blotting problems (reply: 9)
  433. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  434. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  435. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
  436. Non-specific bands on EMSA - (reply: 2)
  437. Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
  438. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  439. PCR ghost bands - (reply: 1)
  440. Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
  441. strange extra bands on digest of miniprep? - (reply: 5)
  442. GST preotin non specific preotins - Non specific GST bands (reply: 2)
  443. 3'RACE and the polyA tail - why only one band? (reply: 2)
  444. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
  445. A single band on WB smaller than expected - (reply: 3)
  446. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  447. Co-IP problem - Weird IgG band (reply: 1)
  448. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
  449. EMSA supershift - increased band intensity? - (reply: 1)
  450. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  451. no band in gel - gel electrophoresis (reply: 1)
  452. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
  453. Strange bands in western of RPE-1 cells - (reply: 1)
  454. no 18S band on RNA agarose gel - (reply: 1)
  455. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  456. multiple bands in my RNA sample? - (reply: 2)
  457. multiple bands in my RNA sample? - (reply: 2)
  458. Ghost band in SDS PAGE - (reply: 2)
  459. No band in the gel after ligation - (reply: 6)
  460. Unidentified DNA bands - (reply: 1)
  461. silica purification - problem with double bands after silica purification (reply: 1)
  462. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  463. normal PCR - two bands is found in one sample (reply: 3)
  464. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  465. After double digestion, one weird band on the very top! - (reply: 4)
  466. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  467. Immunoprecipitation (optimization?) problem - anti-ovalbumin lane has 225 kDa band. (reply: 7)
  468. strange double band in electrophoretic pattern - (reply: 2)
  469. protein band size determination - any software available? (reply: 2)