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Band Related Discussions
  1. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
  2. Band did not migrate on gel electrophoresis (SDS) - (reply: 3)
  3. RNA Gel 3 Sharp bands? - (reply: 1)
  4. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  5. Gel Bands - (reply: 1)
  6. No bands can be seen in my co-IP - (reply: 3)
  7. large bands in all pcr reactions including negative control - (reply: 1)
  8. reason for curved bands? - (reply: 2)
  9. Protein bands visible on unstained SDS-PAGE - (reply: 1)
  10. Digestion bands appear faint - (reply: 3)
  11. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
  12. double band with anti-Flag but not with anti-HA antibody - (reply: 1)
  13. Why Iím not getting any band after column purification? - (reply: 1)
  14. Strange band pattern in Western Blot - (reply: 1)
  15. Western Blot: protein band did not appeared at the correct location - (reply: 2)
  16. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
  17. Mysterious disappearing bands after blotting - (reply: 5)
  18. Western blot with smeared bands - (reply: 3)
  19. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  20. Thick 100-bp band disappeared under UV - (reply: 3)
  21. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  22. Electrophoresis after PCR : too many bands - (reply: 6)
  23. Nanog double band in WB - (reply: 1)
  24. My beta actin bands are changing - (reply: 6)
  25. Why does my gel have streaks instead of bands? - (reply: 2)
  26. Band on Western Blot are smeared - (reply: 1)
  27. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  28. Need help to identify bands in AGE result - (reply: 3)
  29. EMSA Shifted band in Free probe only lane - (reply: 1)
  30. Please help uneven bands in western blot - (reply: 9)
  31. Western Blot Problems...too many unspecific bands - (reply: 4)
  32. I never see the 16kDa band from the seeblue marker? - (reply: 3)
  33. Bands near edges running faster - (reply: 4)
  34. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  35. Smeary bands on agarose gel (also the ladder) - (reply: 2)
  36. No Band With Large DNA (BAC) - (reply: 1)
  37. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
  38. i couldn't get the band of target gene after ligation - (reply: 9)
  39. IP: specific bands in control lanes - (reply: 1)
  40. Multiple Bands after gel extraction - (reply: 4)
  41. DNA extraction problem - strange bands - (reply: 4)
  42. Help!! Double band in genomic dna - (reply: 1)
  43. No bands despite different primers and conditions and TAqs - (reply: 8)
  44. unwanted band - (reply: 5)
  45. Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
  46. strong 100kda bands for alpha tubulin + the usual 55 kda bands? URGENT - (reply: 3)
  47. How to get rid of bands in PCR negative control - (reply: 10)
  48. T7 and M13 primers two band amplification - (reply: 3)
  49. Disappeared bands after the addition of Sigma P2714 - (reply: 1)
  50. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  51. Prey protein band bleeds, IgG band normal? - (reply: 3)
  52. negative control well 300 bp band - (reply: 3)
  53. How to confirm the band of interest in Westerns blot - (reply: 6)
  54. why i get double band for recombinant protein - (reply: 9)
  55. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  56. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
  57. Distorted bands from human colon samples - (reply: 2)
  58. "Wavy" bands in SDS-PAGE gel - (reply: 5)
  59. I get band double the size what does it mean - (reply: 3)
  60. Smeared Bands after Ponceau Staining? - (reply: 3)
  61. RFLP problem - missing band - (reply: 2)
  62. patchy/holey bands after transfer, invasive bubbles? - (reply: 5)
  63. fused bands of beta-actin in western blot - (reply: 2)
  64. how to get specific bands in co-ip - (reply: 5)
  65. faint band after plasmid extraction - (reply: 7)
  66. Undefined bands in Gel - (reply: 9)
  67. are they non-specific band? - (reply: 4)
  68. Trouble with purified recombinant protein bands - (reply: 3)
  69. 3 extra bands show up on my western after an IP - (reply: 2)
  70. Agarose gel bands gone (for gel extraction) - (reply: 2)
  71. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  72. 3' RACE creates too big band doesn't leave well - (reply: 1)
  73. RT-PCR product- no band - (reply: 4)
  74. Wierd Bands after PCR....Confused - (reply: 9)
  75. Quantify DNA in gel electrophoresis bands - (reply: 7)
  76. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
  77. Restriction digest band shift? - (reply: 1)
  78. No bands/weird bands on western blot - (reply: 3)
  79. Straight bands on DNA gel - (reply: 3)
  80. Western blot- bands weak - (reply: 10)
  81. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
  82. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  83. Curved bands in Western Blot - (reply: 2)
  84. Some bands not coming up, others uneven - (reply: 9)
  85. No bands in PCR after DNase treatment - (reply: 4)
  86. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
  87. B- actin band looks like dumbbells! - (reply: 2)
  88. Which antibody is good for HA-tag with low background bands? - (reply: 1)
  89. inespecific bands in nuclear fraction? - (reply: 1)
  90. PCR band slightly BELOW expected length ?! - (reply: 14)
  91. unexpected band in PCR with plasmid - (reply: 4)
  92. cloning: band at different site than desired after RE digestion - (reply: 11)
  93. bands position in agarose gel - (reply: 4)
  94. Multiple Bands in qPCR - (reply: 4)
  95. SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
  96. White bands on Cy3 gels - (reply: 1)
  97. Western Blot, Bax, Many bands - (reply: 3)
  98. Odd Bands on Gel - (reply: 3)
  99. qPCR: band in gel, but super high Cq? - (reply: 10)
  100. SDS Page and WB: Wrong size bands - (reply: 2)
  101. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
  102. Please help with odd bands and smears on Western blot - (reply: 4)
  103. Shift in the position of band onto the western blot - (reply: 3)
  104. Ghost and inverse bands and high background. Help! - (reply: 3)
  105. No resolving of protein/bands below 50kDa :-s - (reply: 5)
  106. "Mickey Mouse" bands - (reply: 5)
  107. aditional band on WB - (reply: 1)
  108. Bands dark on sides and light in middle - (reply: 7)
  109. nonspecific band at 50bp in PCR - (reply: 9)
  110. Protein bands not migrating - (reply: 3)
  111. Single Plasmid Gel Band - (reply: 1)
  112. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
  113. Detecting doublet bands in Western for eGFP control - (reply: 1)
  114. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  115. Uneven bands denaturing agarose - (reply: 2)
  116. two bands on WB-alternative use of start codons? - (reply: 2)
  117. PCR band moves lower than its size on agarose gel - (reply: 3)
  118. No WB bands for H2AX at all...! - (reply: 10)
  119. Overexpressed protein shows a band higher than expected?! - (reply: 2)
  120. Help! No bands after BSP! - (reply: 3)
  121. Multiple bands on blot with one antibody - (reply: 1)
  122. Multiple protein bands after expression - (reply: 6)
  123. Bands in negative control PCR - (reply: 2)
  124. No bands at all after double digestion - (reply: 1)
  125. Non-specific bands and fuzzy bands - (reply: 1)
  126. Weird extra bands in EMSA - (reply: 1)
  127. Help for western blot wet transfer blurry bands - (reply: 2)
  128. Blurry bands ... - (reply: 12)
  129. Strange Bands - (reply: 2)
  130. smearing below band of interest - (reply: 5)
  131. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  132. No or very weak band using 16s primers - (reply: 1)
  133. band shift after IP - (reply: 1)
  134. Western blot narrow bands - (reply: 1)
  135. Bizarre changes in plasmid band size - (reply: 3)
  136. Western blot band anaylisis - (reply: 3)
  137. RNA degradation problem, only one band on the gel - (reply: 3)
  138. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  139. 16s double bands obtained-why?? - (reply: 2)
  140. Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
  141. got band in control positive but not in sample... - (reply: 2)
  142. Band is at the wrong size, but same for all antibodies - (reply: 6)
  143. Puzzeling western blot results, missing band - (reply: 3)
  144. No bands appearing in DNA gel electrophoresis - (reply: 1)
  145. Multiple bands in eluted PCR product - (reply: 1)
  146. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  147. BAC miniprep-high nanodrop value but NO BAND - (reply: 2)
  148. multiple bands for genomic DNA extraction - (reply: 3)
  149. The same strong bands with different primary antibody - (reply: 1)
  150. many bands Western Blot - (reply: 2)
  151. unespecific band PCR from mouse genomic DNA - (reply: 9)
  152. Help with unknown band in restriction digest - (reply: 1)
  153. Preference for larger of 2 bands? - (reply: 2)
  154. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  155. RNase Band in old plasmid stored at -40 - (reply: 2)
  156. getting rid of non specific bands in IP experiments - (reply: 7)
  157. strange bands shape agarose gel - (reply: 2)
  158. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
  159. Getting too Many Bands on my Western Blot - (reply: 9)
  160. Extra bands after protein expression/purification - (reply: 4)
  161. sequencing result from excised DGGE bands - (reply: 3)
  162. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
  163. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  164. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  165. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  166. Analyzing Western Bands by Densiometry - (reply: 2)
  167. Problem: Cloning DNA shuffled band - (reply: 2)
  168. Problems with a PCR-No bands and I am starting to question my sanity - (reply: 4)
  169. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
  170. Problem with PCR - large extra bands - (reply: 3)
  171. Same non specific band for different target proteins - (reply: 1)
  172. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
  173. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
  174. No band in PCR - (reply: 5)
  175. Identically loaded western bands loose intensity from left to right - (reply: 3)
  176. smear bands in western blot - (reply: 12)
  177. bands fuse together - (reply: 1)
  178. Measuring of cDNA bands density - (reply: 3)
  179. No band after extracting DNA from bacillus subtilis - (reply: 1)
  180. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  181. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
  182. Re-using membranes with ghost bands - (reply: 2)
  183. huge dot appears at the centre of every of my bands - (reply: 2)
  184. Bright bands of PCR products stick in gel wells - (reply: 1)
  185. Separating close bands in Western Blot - (reply: 3)
  186. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  187. Bands in my PCR Controls - (reply: 3)
  188. Strange band in colony PCR - (reply: 2)
  189. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  190. Bands seen with qPCR missing in regular PCR - (reply: 5)
  191. non specific bands - (reply: 7)
  192. why cant get the band? - (reply: 4)
  193. Western Blot no target bands, show Non-specific bands - (reply: 5)
  194. Bands disappear after longer exposure - (reply: 4)
  195. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  196. How to remove the background and unspecific bands - (reply: 12)
  197. Same Non-specific bands show up for different Abs!! - (reply: 7)
  198. what caused bubble band of my WB? - (reply: 4)
  199. Strange bands and large smear in western blot - (reply: 4)
  200. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
  201. HELP!- strange band appearing on immunoblot - strange band detected during blotting with anti-CTB antibodies (reply: 3)
  202. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  203. Band Quantitation - Bio-Rad's Quantity One (reply: 1)
  204. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
  205. how to get rid of non specific bands in PCR - (reply: 1)
  206. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
  207. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
  208. SDS PAGE smearing of bands - (reply: 2)
  209. Fuzzy bands in western blot - (reply: 2)
  210. Large Non-specfic bands in PCR - (reply: 3)
  211. HELP!! I ruin my Native-PAGE (newbie in PAGE) - My bands seem faint smear (reply: 3)
  212. 2 bands in blue colony PCR - (reply: 5)
  213. no bands after pcr - even after we have change most of the reagents (reply: 2)
  214. Identify ponceau visible bands - (reply: 1)
  215. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  216. Many unspecific bands after using anti-His on Western blot - (reply: 4)
  217. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  218. KO mice show band for knocked-out protein - (reply: 1)
  219. western blot bands wider at the middle than the top - (reply: 2)
  220. PCR Gel nonspecific band - (reply: 13)
  221. can't get bands in Western Blot - (reply: 5)
  222. difference in band sizes ! - why ??? (reply: 2)
  223. Funny DNA bands on Agarose gels - (reply: 6)
  224. Actin Bands smiling on western blot - (reply: 3)
  225. PCR: always got band in the negative control - (reply: 8)
  226. nonspecific bands on film - (reply: 3)
  227. 'lace-like texture' on my northern blot bands - (reply: 8)
  228. Problem Viewing bands in Agarose Gel - (reply: 6)
  229. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  230. software for quantifing PCR product band - (reply: 1)
  231. Multiple bands from purified PCR product - (reply: 3)
  232. Quantification of proteins in Western Bands - (reply: 2)
  233. No bands on agarose gel after restriction digestion. - (reply: 10)
  234. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  235. Extra band on gel after miniprep - (reply: 6)
  236. 3 distinct bands of gDNA on gel - (reply: 2)
  237. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  238. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  239. Total RNA only produce 1 band in gel - (reply: 2)
  240. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
  241. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
  242. weird bands after minipreps - molecular cloning (reply: 2)
  243. Band at same position using different primary antibodies - (reply: 3)
  244. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  245. PCR, using gel extracted band as a template - (reply: 4)
  246. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  247. PCR bands in NTC but NOT in negative samples - (reply: 2)
  248. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
  249. Unusual band in transformants - (reply: 2)
  250. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  251. Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
  252. no band, extremely clean blot - (reply: 1)
  253. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  254. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  255. IgG - unspecific band (reply: 3)
  256. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
  257. "Unzipped" PCR band - (reply: 3)
  258. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  259. uncut vector contamination in gel purified band - (reply: 2)
  260. I see bands with western blot only the third day! - (reply: 2)
  261. incorrect/smaller band size for real time PCR - (reply: 1)
  262. Agarose gel bands smear/ drag problem - (reply: 4)
  263. Northern - Do not get Bands in Northern Blot (reply: 2)
  264. two bands detected in western - (reply: 1)
  265. Protein SDS-PAGE thick bands / smear - (reply: 4)
  266. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  267. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
  268. need help plz, band sizes estimation - (reply: 2)
  269. What causes this offset band on RIPA gel? - (reply: 4)
  270. nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
  271. band diffusion in sds - (reply: 5)
  272. too much bands in western - (reply: 2)
  273. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  274. Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
  275. Bands are lower than actual protein - (reply: 1)
  276. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  277. No band after WB revelation - (reply: 2)
  278. No band after digestion - (reply: 4)
  279. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
  280. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  281. PCR -No band formation - (reply: 1)
  282. PCR - No Band formation - (reply: 3)
  283. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
  284. 2d page - no band (reply: 2)
  285. never get bands by pfu related enzymes - (reply: 2)
  286. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  287. Lower MW bands than expected. - (reply: 1)
  288. Multiple bands after bisulfite PCR - (reply: 7)
  289. western bands look funny - (reply: 5)
  290. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  291. quantifying "ghost" bands - (reply: 1)
  292. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
  293. Extra bands after QIAGEN spin miniprep - (reply: 3)
  294. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  295. Cold probe doesnt compete away bands - (reply: 2)
  296. Strength of actin signal - How dark should the band be? (reply: 5)
  297. Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
  298. PCR product appear two close band in my gel - (reply: 15)
  299. The Case of a Missing Band: PCR Issue - (reply: 4)
  300. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  301. Band appear and disapper in the negative control ?? - (reply: 2)
  302. Bands in PCR negative control - (reply: 3)
  303. Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
  304. western exposure one part of band darker - (reply: 6)
  305. non-specific bands - (reply: 5)
  306. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  307. bands of protein seems like it did not move - (reply: 2)
  308. downshifted band - (reply: 4)
  309. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
  310. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
  311. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  312. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  313. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
  314. more than one band - (reply: 1)
  315. IGg bands on SDS PAGE - (reply: 2)
  316. PCR band too thick - PCR troubleshooting question (reply: 5)
  317. ugly merged bands in western blot - (reply: 3)
  318. PCR bands on gel electrophoresis - (reply: 5)
  319. Identify the band in western blot - (reply: 5)
  320. Ponceau staining - Appearence of bands (reply: 1)
  321. Multiple bands after amping insert - (reply: 2)
  322. Protein bands on the bottom of the gel - (reply: 5)
  323. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
  324. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
  325. Bands in a miRNA gel - (reply: 1)
  326. Gel Electrophoresis band problems - (reply: 3)
  327. Problem with protein samples - No bands detected at all on gel (reply: 2)
  328. Unhappy bands - (reply: 3)
  329. High MW PCR band seen...help needed - (reply: 7)
  330. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
  331. HELP! Genomic DNA, RNA-size bands - (reply: 2)
  332. No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
  333. "Diffuesed" bands of higher molecular weight - (reply: 6)
  334. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  335. strange band in coomassie blue staining - (reply: 3)
  336. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  337. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  338. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  339. Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
  340. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  341. Wrong bands on the blot - (reply: 4)
  342. Problems detecting my band! - (reply: 2)
  343. Western bands fading out, pleeeese help - (reply: 1)
  344. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
  345. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
  346. Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
  347. Bands visible in membrane but not in the film - ?? (reply: 10)
  348. non desired brigther bands - (reply: 7)
  349. Bands on adjescent lanes!!! - wierd!!! (reply: 11)
  350. Only DNA ladder , No desired band in PCR - (reply: 4)
  351. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  352. Lost a Band by changing thermocycler - (reply: 9)
  353. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
  354. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  355. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  356. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  357. Bands appearing out of no where?! - (reply: 5)
  358. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  359. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
  360. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  361. casting agarose gel troubleshooting - smeared bands (reply: 8)
  362. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  363. Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
  364. the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
  365. overloading GAPDH but nice band of interest - (reply: 2)
  366. Quantiying bands. - Determination of protein contens (reply: 2)
  367. after re-isolation of vector, get single band - (reply: 4)
  368. BSP PCR for help - no bands of tissue amplification (reply: 3)
  369. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  370. Too many bands in my SDS-PAGE - (reply: 5)
  371. Uneven bands in SDS-PAGE - (reply: 5)
  372. weak bands for methylated DNA - (reply: 4)
  373. Two bands for p53 on Western Blot - (reply: 7)
  374. nonspecific bands after co-ip - (reply: 2)
  375. No PCR bands with some templates - (reply: 5)
  376. EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
  377. Blurred low molecular weight bands in SDS-PAGE - Need your help (reply: 1)
  378. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
  379. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
  380. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
  381. light bands, dark smear, dark dimers - (reply: 2)
  382. pcr doesnt show any band for DNa walking - (reply: 8)
  383. Immunoprecipitation - Bands in negative control - (reply: 2)
  384. crappy bands after developing - (reply: 3)
  385. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  386. no bands in my PCR - (reply: 9)
  387. All gradient samples give bands! How come? - (reply: 4)
  388. many bands of smaller size than expected - (reply: 3)
  389. wrong bands after T-vector cloning - (reply: 3)
  390. abnormal band in agarose gel - Urgent help required (reply: 3)
  391. Ghost bands in western blot - Western blotting problems (reply: 9)
  392. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  393. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  394. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
  395. Non-specific bands on EMSA - (reply: 2)
  396. Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
  397. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  398. PCR ghost bands - (reply: 1)
  399. Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
  400. strange extra bands on digest of miniprep? - (reply: 5)
  401. GST preotin non specific preotins - Non specific GST bands (reply: 2)
  402. 3'RACE and the polyA tail - why only one band? (reply: 2)
  403. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
  404. A single band on WB smaller than expected - (reply: 3)
  405. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  406. Co-IP problem - Weird IgG band (reply: 1)
  407. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
  408. EMSA supershift - increased band intensity? - (reply: 1)
  409. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  410. no band in gel - gel electrophoresis (reply: 1)
  411. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
  412. Strange bands in western of RPE-1 cells - (reply: 1)
  413. no 18S band on RNA agarose gel - (reply: 1)
  414. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  415. multiple bands in my RNA sample? - (reply: 2)
  416. multiple bands in my RNA sample? - (reply: 2)
  417. Ghost band in SDS PAGE - (reply: 2)
  418. No band in the gel after ligation - (reply: 6)
  419. Unidentified DNA bands - (reply: 1)
  420. silica purification - problem with double bands after silica purification (reply: 1)
  421. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  422. normal PCR - two bands is found in one sample (reply: 3)
  423. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  424. After double digestion, one weird band on the very top! - (reply: 4)
  425. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  426. Immunoprecipitation (optimization?) problem - anti-ovalbumin lane has 225 kDa band. (reply: 7)
  427. strange double band in electrophoretic pattern - (reply: 2)
  428. protein band size determination - any software available? (reply: 2)