Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Band Related Discussions
  1. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
  2. All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
  3. RNA gel problem (melted bands) - (reply: 2)
  4. The slightly bigger band dillema. - (reply: 2)
  5. Non especific band in PCR... - (reply: 2)
  6. Smeary bands in gel electrophoresis - (reply: 3)
  7. band obove the ladder - (reply: 3)
  8. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  9. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 1)
  10. unexpected protein bands - (reply: 2)
  11. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
  12. Band did not migrate on gel electrophoresis (SDS) - (reply: 3)
  13. RNA Gel 3 Sharp bands? - (reply: 1)
  14. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  15. Gel Bands - (reply: 1)
  16. No bands can be seen in my co-IP - (reply: 3)
  17. large bands in all pcr reactions including negative control - (reply: 1)
  18. reason for curved bands? - (reply: 2)
  19. Protein bands visible on unstained SDS-PAGE - (reply: 1)
  20. Digestion bands appear faint - (reply: 3)
  21. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
  22. double band with anti-Flag but not with anti-HA antibody - (reply: 1)
  23. Why Iím not getting any band after column purification? - (reply: 1)
  24. Strange band pattern in Western Blot - (reply: 1)
  25. Western Blot: protein band did not appeared at the correct location - (reply: 3)
  26. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
  27. Mysterious disappearing bands after blotting - (reply: 5)
  28. Western blot with smeared bands - (reply: 3)
  29. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  30. Thick 100-bp band disappeared under UV - (reply: 3)
  31. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  32. Electrophoresis after PCR : too many bands - (reply: 6)
  33. Nanog double band in WB - (reply: 1)
  34. My beta actin bands are changing - (reply: 6)
  35. Why does my gel have streaks instead of bands? - (reply: 2)
  36. Band on Western Blot are smeared - (reply: 1)
  37. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  38. Need help to identify bands in AGE result - (reply: 3)
  39. EMSA Shifted band in Free probe only lane - (reply: 1)
  40. Please help uneven bands in western blot - (reply: 9)
  41. Western Blot Problems...too many unspecific bands - (reply: 4)
  42. I never see the 16kDa band from the seeblue marker? - (reply: 3)
  43. Bands near edges running faster - (reply: 4)
  44. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  45. Smeary bands on agarose gel (also the ladder) - (reply: 2)
  46. No Band With Large DNA (BAC) - (reply: 1)
  47. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
  48. i couldn't get the band of target gene after ligation - (reply: 9)
  49. IP: specific bands in control lanes - (reply: 1)
  50. Multiple Bands after gel extraction - (reply: 4)
  51. DNA extraction problem - strange bands - (reply: 4)
  52. Help!! Double band in genomic dna - (reply: 1)
  53. No bands despite different primers and conditions and TAqs - (reply: 8)
  54. unwanted band - (reply: 5)
  55. Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
  56. strong 100kda bands for alpha tubulin + the usual 55 kda bands? URGENT - (reply: 3)
  57. How to get rid of bands in PCR negative control - (reply: 10)
  58. T7 and M13 primers two band amplification - (reply: 3)
  59. Disappeared bands after the addition of Sigma P2714 - (reply: 1)
  60. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  61. Prey protein band bleeds, IgG band normal? - (reply: 3)
  62. negative control well 300 bp band - (reply: 3)
  63. How to confirm the band of interest in Westerns blot - (reply: 6)
  64. why i get double band for recombinant protein - (reply: 9)
  65. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  66. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
  67. Distorted bands from human colon samples - (reply: 2)
  68. "Wavy" bands in SDS-PAGE gel - (reply: 5)
  69. I get band double the size what does it mean - (reply: 3)
  70. Smeared Bands after Ponceau Staining? - (reply: 3)
  71. RFLP problem - missing band - (reply: 2)
  72. patchy/holey bands after transfer, invasive bubbles? - (reply: 5)
  73. fused bands of beta-actin in western blot - (reply: 2)
  74. how to get specific bands in co-ip - (reply: 5)
  75. faint band after plasmid extraction - (reply: 7)
  76. Undefined bands in Gel - (reply: 9)
  77. are they non-specific band? - (reply: 4)
  78. Trouble with purified recombinant protein bands - (reply: 3)
  79. 3 extra bands show up on my western after an IP - (reply: 2)
  80. Agarose gel bands gone (for gel extraction) - (reply: 2)
  81. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  82. 3' RACE creates too big band doesn't leave well - (reply: 1)
  83. RT-PCR product- no band - (reply: 4)
  84. Wierd Bands after PCR....Confused - (reply: 9)
  85. Quantify DNA in gel electrophoresis bands - (reply: 7)
  86. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
  87. Restriction digest band shift? - (reply: 1)
  88. No bands/weird bands on western blot - (reply: 3)
  89. Straight bands on DNA gel - (reply: 3)
  90. Western blot- bands weak - (reply: 10)
  91. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
  92. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  93. Curved bands in Western Blot - (reply: 2)
  94. Some bands not coming up, others uneven - (reply: 9)
  95. No bands in PCR after DNase treatment - (reply: 4)
  96. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
  97. B- actin band looks like dumbbells! - (reply: 2)
  98. Which antibody is good for HA-tag with low background bands? - (reply: 1)
  99. inespecific bands in nuclear fraction? - (reply: 1)
  100. PCR band slightly BELOW expected length ?! - (reply: 14)
  101. unexpected band in PCR with plasmid - (reply: 4)
  102. cloning: band at different site than desired after RE digestion - (reply: 11)
  103. bands position in agarose gel - (reply: 4)
  104. Multiple Bands in qPCR - (reply: 4)
  105. SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
  106. White bands on Cy3 gels - (reply: 1)
  107. Western Blot, Bax, Many bands - (reply: 3)
  108. Odd Bands on Gel - (reply: 3)
  109. qPCR: band in gel, but super high Cq? - (reply: 10)
  110. SDS Page and WB: Wrong size bands - (reply: 2)
  111. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
  112. Please help with odd bands and smears on Western blot - (reply: 4)
  113. Shift in the position of band onto the western blot - (reply: 3)
  114. Ghost and inverse bands and high background. Help! - (reply: 3)
  115. No resolving of protein/bands below 50kDa :-s - (reply: 5)
  116. "Mickey Mouse" bands - (reply: 5)
  117. aditional band on WB - (reply: 1)
  118. Bands dark on sides and light in middle - (reply: 7)
  119. nonspecific band at 50bp in PCR - (reply: 9)
  120. Protein bands not migrating - (reply: 3)
  121. Single Plasmid Gel Band - (reply: 1)
  122. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
  123. Detecting doublet bands in Western for eGFP control - (reply: 1)
  124. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  125. Uneven bands denaturing agarose - (reply: 2)
  126. two bands on WB-alternative use of start codons? - (reply: 2)
  127. PCR band moves lower than its size on agarose gel - (reply: 3)
  128. No WB bands for H2AX at all...! - (reply: 10)
  129. Overexpressed protein shows a band higher than expected?! - (reply: 2)
  130. Help! No bands after BSP! - (reply: 3)
  131. Multiple bands on blot with one antibody - (reply: 1)
  132. Multiple protein bands after expression - (reply: 6)
  133. Bands in negative control PCR - (reply: 2)
  134. No bands at all after double digestion - (reply: 1)
  135. Non-specific bands and fuzzy bands - (reply: 1)
  136. Weird extra bands in EMSA - (reply: 1)
  137. Help for western blot wet transfer blurry bands - (reply: 2)
  138. Blurry bands ... - (reply: 12)
  139. Strange Bands - (reply: 2)
  140. smearing below band of interest - (reply: 5)
  141. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  142. No or very weak band using 16s primers - (reply: 1)
  143. band shift after IP - (reply: 1)
  144. Western blot narrow bands - (reply: 1)
  145. Bizarre changes in plasmid band size - (reply: 3)
  146. Western blot band anaylisis - (reply: 3)
  147. RNA degradation problem, only one band on the gel - (reply: 3)
  148. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  149. 16s double bands obtained-why?? - (reply: 2)
  150. Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
  151. got band in control positive but not in sample... - (reply: 2)
  152. Band is at the wrong size, but same for all antibodies - (reply: 6)
  153. Puzzeling western blot results, missing band - (reply: 3)
  154. No bands appearing in DNA gel electrophoresis - (reply: 1)
  155. Multiple bands in eluted PCR product - (reply: 1)
  156. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  157. BAC miniprep-high nanodrop value but NO BAND - (reply: 2)
  158. multiple bands for genomic DNA extraction - (reply: 3)
  159. The same strong bands with different primary antibody - (reply: 1)
  160. many bands Western Blot - (reply: 2)
  161. unespecific band PCR from mouse genomic DNA - (reply: 9)
  162. Help with unknown band in restriction digest - (reply: 1)
  163. Preference for larger of 2 bands? - (reply: 2)
  164. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  165. RNase Band in old plasmid stored at -40 - (reply: 2)
  166. getting rid of non specific bands in IP experiments - (reply: 7)
  167. strange bands shape agarose gel - (reply: 2)
  168. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
  169. Getting too Many Bands on my Western Blot - (reply: 9)
  170. Extra bands after protein expression/purification - (reply: 4)
  171. sequencing result from excised DGGE bands - (reply: 3)
  172. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
  173. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  174. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  175. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  176. Analyzing Western Bands by Densiometry - (reply: 2)
  177. Problem: Cloning DNA shuffled band - (reply: 2)
  178. Problems with a PCR-No bands and I am starting to question my sanity - (reply: 4)
  179. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
  180. Problem with PCR - large extra bands - (reply: 3)
  181. Same non specific band for different target proteins - (reply: 1)
  182. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
  183. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
  184. No band in PCR - (reply: 5)
  185. Identically loaded western bands loose intensity from left to right - (reply: 3)
  186. smear bands in western blot - (reply: 12)
  187. bands fuse together - (reply: 1)
  188. Measuring of cDNA bands density - (reply: 3)
  189. No band after extracting DNA from bacillus subtilis - (reply: 1)
  190. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  191. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
  192. Re-using membranes with ghost bands - (reply: 2)
  193. huge dot appears at the centre of every of my bands - (reply: 2)
  194. Bright bands of PCR products stick in gel wells - (reply: 1)
  195. Separating close bands in Western Blot - (reply: 3)
  196. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  197. Bands in my PCR Controls - (reply: 3)
  198. Strange band in colony PCR - (reply: 2)
  199. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  200. Bands seen with qPCR missing in regular PCR - (reply: 5)
  201. non specific bands - (reply: 7)
  202. why cant get the band? - (reply: 4)
  203. Western Blot no target bands, show Non-specific bands - (reply: 5)
  204. Bands disappear after longer exposure - (reply: 4)
  205. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  206. How to remove the background and unspecific bands - (reply: 12)
  207. Same Non-specific bands show up for different Abs!! - (reply: 7)
  208. what caused bubble band of my WB? - (reply: 4)
  209. Strange bands and large smear in western blot - (reply: 4)
  210. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
  211. HELP!- strange band appearing on immunoblot - strange band detected during blotting with anti-CTB antibodies (reply: 3)
  212. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  213. Band Quantitation - Bio-Rad's Quantity One (reply: 1)
  214. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
  215. how to get rid of non specific bands in PCR - (reply: 1)
  216. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
  217. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
  218. SDS PAGE smearing of bands - (reply: 2)
  219. Fuzzy bands in western blot - (reply: 2)
  220. Large Non-specfic bands in PCR - (reply: 3)
  221. HELP!! I ruin my Native-PAGE (newbie in PAGE) - My bands seem faint smear (reply: 3)
  222. 2 bands in blue colony PCR - (reply: 5)
  223. no bands after pcr - even after we have change most of the reagents (reply: 2)
  224. Identify ponceau visible bands - (reply: 1)
  225. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  226. Many unspecific bands after using anti-His on Western blot - (reply: 4)
  227. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  228. KO mice show band for knocked-out protein - (reply: 1)
  229. western blot bands wider at the middle than the top - (reply: 2)
  230. PCR Gel nonspecific band - (reply: 13)
  231. can't get bands in Western Blot - (reply: 5)
  232. difference in band sizes ! - why ??? (reply: 2)
  233. Funny DNA bands on Agarose gels - (reply: 6)
  234. Actin Bands smiling on western blot - (reply: 3)
  235. PCR: always got band in the negative control - (reply: 8)
  236. nonspecific bands on film - (reply: 3)
  237. 'lace-like texture' on my northern blot bands - (reply: 8)
  238. Problem Viewing bands in Agarose Gel - (reply: 6)
  239. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  240. software for quantifing PCR product band - (reply: 1)
  241. Multiple bands from purified PCR product - (reply: 3)
  242. Quantification of proteins in Western Bands - (reply: 2)
  243. No bands on agarose gel after restriction digestion. - (reply: 10)
  244. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  245. Extra band on gel after miniprep - (reply: 6)
  246. 3 distinct bands of gDNA on gel - (reply: 2)
  247. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  248. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  249. Total RNA only produce 1 band in gel - (reply: 2)
  250. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
  251. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
  252. weird bands after minipreps - molecular cloning (reply: 2)
  253. Band at same position using different primary antibodies - (reply: 3)
  254. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  255. PCR, using gel extracted band as a template - (reply: 4)
  256. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  257. PCR bands in NTC but NOT in negative samples - (reply: 2)
  258. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
  259. Unusual band in transformants - (reply: 2)
  260. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  261. Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
  262. no band, extremely clean blot - (reply: 1)
  263. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  264. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  265. IgG - unspecific band (reply: 3)
  266. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
  267. "Unzipped" PCR band - (reply: 3)
  268. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  269. uncut vector contamination in gel purified band - (reply: 2)
  270. I see bands with western blot only the third day! - (reply: 2)
  271. incorrect/smaller band size for real time PCR - (reply: 1)
  272. Agarose gel bands smear/ drag problem - (reply: 4)
  273. Northern - Do not get Bands in Northern Blot (reply: 2)
  274. two bands detected in western - (reply: 1)
  275. Protein SDS-PAGE thick bands / smear - (reply: 4)
  276. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  277. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
  278. need help plz, band sizes estimation - (reply: 2)
  279. What causes this offset band on RIPA gel? - (reply: 4)
  280. nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
  281. band diffusion in sds - (reply: 5)
  282. too much bands in western - (reply: 2)
  283. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  284. Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
  285. Bands are lower than actual protein - (reply: 1)
  286. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  287. No band after WB revelation - (reply: 2)
  288. No band after digestion - (reply: 4)
  289. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
  290. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  291. PCR -No band formation - (reply: 1)
  292. PCR - No Band formation - (reply: 3)
  293. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
  294. 2d page - no band (reply: 2)
  295. never get bands by pfu related enzymes - (reply: 2)
  296. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  297. Lower MW bands than expected. - (reply: 1)
  298. Multiple bands after bisulfite PCR - (reply: 7)
  299. western bands look funny - (reply: 5)
  300. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  301. quantifying "ghost" bands - (reply: 1)
  302. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
  303. Extra bands after QIAGEN spin miniprep - (reply: 3)
  304. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  305. Cold probe doesnt compete away bands - (reply: 2)
  306. Strength of actin signal - How dark should the band be? (reply: 5)
  307. Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
  308. PCR product appear two close band in my gel - (reply: 15)
  309. The Case of a Missing Band: PCR Issue - (reply: 4)
  310. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  311. Band appear and disapper in the negative control ?? - (reply: 2)
  312. Bands in PCR negative control - (reply: 3)
  313. Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
  314. western exposure one part of band darker - (reply: 6)
  315. non-specific bands - (reply: 5)
  316. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  317. bands of protein seems like it did not move - (reply: 2)
  318. downshifted band - (reply: 4)
  319. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
  320. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
  321. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  322. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  323. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
  324. more than one band - (reply: 1)
  325. IGg bands on SDS PAGE - (reply: 2)
  326. PCR band too thick - PCR troubleshooting question (reply: 5)
  327. ugly merged bands in western blot - (reply: 3)
  328. PCR bands on gel electrophoresis - (reply: 5)
  329. Identify the band in western blot - (reply: 5)
  330. Ponceau staining - Appearence of bands (reply: 1)
  331. Multiple bands after amping insert - (reply: 2)
  332. Protein bands on the bottom of the gel - (reply: 5)
  333. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
  334. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
  335. Bands in a miRNA gel - (reply: 1)
  336. Gel Electrophoresis band problems - (reply: 3)
  337. Problem with protein samples - No bands detected at all on gel (reply: 2)
  338. Unhappy bands - (reply: 3)
  339. High MW PCR band seen...help needed - (reply: 7)
  340. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
  341. HELP! Genomic DNA, RNA-size bands - (reply: 2)
  342. No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
  343. "Diffuesed" bands of higher molecular weight - (reply: 6)
  344. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  345. strange band in coomassie blue staining - (reply: 3)
  346. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  347. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  348. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  349. Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
  350. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  351. Wrong bands on the blot - (reply: 4)
  352. Problems detecting my band! - (reply: 2)
  353. Western bands fading out, pleeeese help - (reply: 1)
  354. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
  355. Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
  356. Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
  357. Bands visible in membrane but not in the film - ?? (reply: 10)
  358. non desired brigther bands - (reply: 7)
  359. Bands on adjescent lanes!!! - wierd!!! (reply: 11)
  360. Only DNA ladder , No desired band in PCR - (reply: 4)
  361. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  362. Lost a Band by changing thermocycler - (reply: 9)
  363. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
  364. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  365. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  366. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  367. Bands appearing out of no where?! - (reply: 5)
  368. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  369. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
  370. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  371. casting agarose gel troubleshooting - smeared bands (reply: 8)
  372. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  373. Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
  374. the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
  375. overloading GAPDH but nice band of interest - (reply: 2)
  376. Quantiying bands. - Determination of protein contens (reply: 2)
  377. after re-isolation of vector, get single band - (reply: 4)
  378. BSP PCR for help - no bands of tissue amplification (reply: 3)
  379. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  380. Too many bands in my SDS-PAGE - (reply: 5)
  381. Uneven bands in SDS-PAGE - (reply: 5)
  382. weak bands for methylated DNA - (reply: 4)
  383. Two bands for p53 on Western Blot - (reply: 7)
  384. nonspecific bands after co-ip - (reply: 2)
  385. No PCR bands with some templates - (reply: 5)
  386. EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
  387. Blurred low molecular weight bands in SDS-PAGE - Need your help (reply: 1)
  388. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
  389. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
  390. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
  391. light bands, dark smear, dark dimers - (reply: 2)
  392. pcr doesnt show any band for DNa walking - (reply: 8)
  393. Immunoprecipitation - Bands in negative control - (reply: 2)
  394. crappy bands after developing - (reply: 3)
  395. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  396. no bands in my PCR - (reply: 9)
  397. All gradient samples give bands! How come? - (reply: 4)
  398. many bands of smaller size than expected - (reply: 3)
  399. wrong bands after T-vector cloning - (reply: 3)
  400. abnormal band in agarose gel - Urgent help required (reply: 3)
  401. Ghost bands in western blot - Western blotting problems (reply: 9)
  402. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  403. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  404. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
  405. Non-specific bands on EMSA - (reply: 2)
  406. Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
  407. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  408. PCR ghost bands - (reply: 1)
  409. Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
  410. strange extra bands on digest of miniprep? - (reply: 5)
  411. GST preotin non specific preotins - Non specific GST bands (reply: 2)
  412. 3'RACE and the polyA tail - why only one band? (reply: 2)
  413. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
  414. A single band on WB smaller than expected - (reply: 3)
  415. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  416. Co-IP problem - Weird IgG band (reply: 1)
  417. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
  418. EMSA supershift - increased band intensity? - (reply: 1)
  419. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  420. no band in gel - gel electrophoresis (reply: 1)
  421. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
  422. Strange bands in western of RPE-1 cells - (reply: 1)
  423. no 18S band on RNA agarose gel - (reply: 1)
  424. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  425. multiple bands in my RNA sample? - (reply: 2)
  426. multiple bands in my RNA sample? - (reply: 2)
  427. Ghost band in SDS PAGE - (reply: 2)
  428. No band in the gel after ligation - (reply: 6)
  429. Unidentified DNA bands - (reply: 1)
  430. silica purification - problem with double bands after silica purification (reply: 1)
  431. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  432. normal PCR - two bands is found in one sample (reply: 3)
  433. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  434. After double digestion, one weird band on the very top! - (reply: 4)
  435. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  436. Immunoprecipitation (optimization?) problem - anti-ovalbumin lane has 225 kDa band. (reply: 7)
  437. strange double band in electrophoretic pattern - (reply: 2)
  438. protein band size determination - any software available? (reply: 2)