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Band Related Discussions
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3 extra bands show up on my western after an IP - (reply: 2)
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Agarose gel bands gone (for gel extraction) - (reply: 2)
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Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
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3' RACE creates too big band doesn't leave well - (reply: 1)
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RT-PCR product- no band - (reply: 4)
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Wierd Bands after PCR....Confused - (reply: 9)
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Quantify DNA in gel electrophoresis bands - (reply: 7)
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Western Blot: only non-specific bands and not protein of interest - (reply: 3)
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Restriction digest band shift? - (reply: 1)
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No bands/weird bands on western blot - (reply: 3)
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Straight bands on DNA gel - (reply: 3)
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Western blot- bands weak - (reply: 10)
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DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
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10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
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Curved bands in Western Blot - (reply: 2)
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Some bands not coming up, others uneven - (reply: 9)
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No bands in PCR after DNase treatment - (reply: 4)
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Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
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B- actin band looks like dumbbells! - (reply: 2)
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Which antibody is good for HA-tag with low background bands? - (reply: 1)
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inespecific bands in nuclear fraction? - (reply: 1)
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PCR band slightly BELOW expected length ?! - (reply: 14)
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unexpected band in PCR with plasmid - (reply: 4)
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cloning: band at different site than desired after RE digestion - (reply: 11)
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bands position in agarose gel - (reply: 4)
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Multiple Bands in qPCR - (reply: 4)
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SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
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White bands on Cy3 gels - (reply: 1)
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Western Blot, Bax, Many bands - (reply: 3)
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Odd Bands on Gel - (reply: 3)
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qPCR: band in gel, but super high Cq? - (reply: 10)
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SDS Page and WB: Wrong size bands - (reply: 2)
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myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
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Please help with odd bands and smears on Western blot - (reply: 4)
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Shift in the position of band onto the western blot - (reply: 3)
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Ghost and inverse bands and high background. Help! - (reply: 3)
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No resolving of protein/bands below 50kDa :-s - (reply: 5)
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"Mickey Mouse" bands - (reply: 5)
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aditional band on WB - (reply: 1)
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Bands dark on sides and light in middle - (reply: 7)
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nonspecific band at 50bp in PCR - (reply: 9)
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Protein bands not migrating - (reply: 3)
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Single Plasmid Gel Band - (reply: 1)
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Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
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Detecting doublet bands in Western for eGFP control - (reply: 1)
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Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
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Uneven bands denaturing agarose - (reply: 2)
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two bands on WB-alternative use of start codons? - (reply: 2)
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PCR band moves lower than its size on agarose gel - (reply: 3)
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No WB bands for H2AX at all...! - (reply: 10)
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Overexpressed protein shows a band higher than expected?! - (reply: 2)
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Help! No bands after BSP! - (reply: 2)
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Multiple bands on blot with one antibody - (reply: 1)
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Multiple protein bands after expression - (reply: 6)
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Bands in negative control PCR - (reply: 2)
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No bands at all after double digestion - (reply: 1)
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Non-specific bands and fuzzy bands - (reply: 1)
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Weird extra bands in EMSA - (reply: 1)
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Help for western blot wet transfer blurry bands - (reply: 2)
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Blurry bands ... - (reply: 12)
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Strange Bands - (reply: 2)
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smearing below band of interest - (reply: 5)
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After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
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No or very weak band using 16s primers - (reply: 1)
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band shift after IP - (reply: 1)
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Western blot narrow bands - (reply: 1)
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Bizarre changes in plasmid band size - (reply: 3)
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Western blot band anaylisis - (reply: 3)
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RNA degradation problem, only one band on the gel - (reply: 3)
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Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
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16s double bands obtained-why?? - (reply: 2)
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Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
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got band in control positive but not in sample... - (reply: 2)
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Band is at the wrong size, but same for all antibodies - (reply: 6)
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Puzzeling western blot results, missing band - (reply: 3)
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No bands appearing in DNA gel electrophoresis - (reply: 1)
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Multiple bands in eluted PCR product - (reply: 1)
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having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
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BAC miniprep-high nanodrop value but NO BAND - (reply: 2)
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multiple bands for genomic DNA extraction - (reply: 3)
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The same strong bands with different primary antibody - (reply: 1)
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many bands Western Blot - (reply: 2)
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unespecific band PCR from mouse genomic DNA - (reply: 9)
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Help with unknown band in restriction digest - (reply: 1)
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Preference for larger of 2 bands? - (reply: 2)
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PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
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RNase Band in old plasmid stored at -40 - (reply: 2)
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getting rid of non specific bands in IP experiments - (reply: 7)
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strange bands shape agarose gel - (reply: 2)
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MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
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Getting too Many Bands on my Western Blot - (reply: 9)
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Extra bands after protein expression/purification - (reply: 4)
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sequencing result from excised DGGE bands - (reply: 3)
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Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
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Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
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Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
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Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
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Analyzing Western Bands by Densiometry - (reply: 2)
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Problem: Cloning DNA shuffled band - (reply: 2)
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Problems with a PCR-No bands and I am starting to question my sanity - (reply: 4)
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my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
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Problem with PCR - large extra bands - (reply: 3)
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Same non specific band for different target proteins - (reply: 1)
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a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
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Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
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No band in PCR - (reply: 5)
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Identically loaded western bands loose intensity from left to right - (reply: 3)
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smear bands in western blot - (reply: 12)
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bands fuse together - (reply: 1)
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Measuring of cDNA bands density - (reply: 3)
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No band after extracting DNA from bacillus subtilis - (reply: 1)
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Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
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Bands from the first detection appear at the second detection but with a differe - (reply: 1)
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Re-using membranes with ghost bands - (reply: 2)
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huge dot appears at the centre of every of my bands - (reply: 2)
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Bright bands of PCR products stick in gel wells - (reply: 1)
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Separating close bands in Western Blot - (reply: 3)
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Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
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Bands in my PCR Controls - (reply: 3)
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Strange band in colony PCR - (reply: 2)
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overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
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Bands seen with qPCR missing in regular PCR - (reply: 5)
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non specific bands - (reply: 7)
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why cant get the band? - (reply: 4)
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Western Blot no target bands, show Non-specific bands - (reply: 5)
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Bands disappear after longer exposure - (reply: 4)
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clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
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How to remove the background and unspecific bands - (reply: 12)
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Same Non-specific bands show up for different Abs!! - (reply: 7)
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what caused bubble band of my WB? - (reply: 4)
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Strange bands and large smear in western blot - (reply: 4)
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GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
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HELP!- strange band appearing on immunoblot - strange band detected during blotting with anti-CTB antibodies (reply: 3)
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PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
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Band Quantitation - Bio-Rad's Quantity One (reply: 1)
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strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
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how to get rid of non specific bands in PCR - (reply: 1)
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Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
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Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
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SDS PAGE smearing of bands - (reply: 2)
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Fuzzy bands in western blot - (reply: 2)
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Large Non-specfic bands in PCR - (reply: 3)
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HELP!! I ruin my Native-PAGE (newbie in PAGE) - My bands seem faint smear (reply: 3)
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2 bands in blue colony PCR - (reply: 5)
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no bands after pcr - even after we have change most of the reagents (reply: 2)
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Identify ponceau visible bands - (reply: 1)
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Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
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Many unspecific bands after using anti-His on Western blot - (reply: 4)
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no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
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KO mice show band for knocked-out protein - (reply: 1)
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western blot bands wider at the middle than the top - (reply: 2)
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PCR Gel nonspecific band - (reply: 13)
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can't get bands in Western Blot - (reply: 5)
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difference in band sizes ! - why ??? (reply: 2)
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Funny DNA bands on Agarose gels - (reply: 6)
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Actin Bands smiling on western blot - (reply: 3)
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PCR: always got band in the negative control - (reply: 8)
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nonspecific bands on film - (reply: 3)
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'lace-like texture' on my northern blot bands - (reply: 8)
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Problem Viewing bands in Agarose Gel - (reply: 6)
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Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
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software for quantifing PCR product band - (reply: 1)
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Multiple bands from purified PCR product - (reply: 3)
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Quantification of proteins in Western Bands - (reply: 2)
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No bands on agarose gel after restriction digestion. - (reply: 10)
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Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
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Extra band on gel after miniprep - (reply: 6)
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3 distinct bands of gDNA on gel - (reply: 2)
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Fat bands on 15% SDS-PAGE gel - (reply: 10)
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Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
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Total RNA only produce 1 band in gel - (reply: 2)
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Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 3)
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how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
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weird bands after minipreps - molecular cloning (reply: 2)
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Band at same position using different primary antibodies - (reply: 3)
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purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
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PCR, using gel extracted band as a template - (reply: 4)
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Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
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PCR bands in NTC but NOT in negative samples - (reply: 2)
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Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
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Unusual band in transformants - (reply: 2)
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mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
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Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
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no band, extremely clean blot - (reply: 1)
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Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
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Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
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IgG - unspecific band (reply: 3)
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SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
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"Unzipped" PCR band - (reply: 3)
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Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
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uncut vector contamination in gel purified band - (reply: 2)
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I see bands with western blot only the third day! - (reply: 2)
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incorrect/smaller band size for real time PCR - (reply: 1)
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Agarose gel bands smear/ drag problem - (reply: 4)
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Northern - Do not get Bands in Northern Blot (reply: 2)
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two bands detected in western - (reply: 1)
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Protein SDS-PAGE thick bands / smear - (reply: 4)
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No protein lane have bands on Coomassie and western blot?! - (reply: 4)
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New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
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need help plz, band sizes estimation - (reply: 2)
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What causes this offset band on RIPA gel? - (reply: 4)
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nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
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band diffusion in sds - (reply: 5)
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too much bands in western - (reply: 2)
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Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
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Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
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Bands are lower than actual protein - (reply: 1)
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PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
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No band after WB revelation - (reply: 2)
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No band after digestion - (reply: 4)
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fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
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electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
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PCR -No band formation - (reply: 1)
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PCR - No Band formation - (reply: 3)
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No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
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2d page - no band (reply: 2)
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never get bands by pfu related enzymes - (reply: 2)
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DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
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Lower MW bands than expected. - (reply: 1)
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Multiple bands after bisulfite PCR - (reply: 7)
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western bands look funny - (reply: 5)
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SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
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quantifying "ghost" bands - (reply: 1)
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Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
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Extra bands after QIAGEN spin miniprep - (reply: 3)
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agarose gel of ligated DNA doesn't show any bands - (reply: 2)
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Cold probe doesnt compete away bands - (reply: 2)
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Strength of actin signal - How dark should the band be? (reply: 5)
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Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
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PCR product appear two close band in my gel - (reply: 15)
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The Case of a Missing Band: PCR Issue - (reply: 4)
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PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
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Band appear and disapper in the negative control ?? - (reply: 2)
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Bands in PCR negative control - (reply: 3)
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Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
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western exposure one part of band darker - (reply: 6)
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non-specific bands - (reply: 5)
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Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
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bands of protein seems like it did not move - (reply: 2)
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downshifted band - (reply: 4)
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no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
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problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
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3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
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Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
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PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
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more than one band - (reply: 1)
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IGg bands on SDS PAGE - (reply: 2)
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PCR band too thick - PCR troubleshooting question (reply: 5)
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ugly merged bands in western blot - (reply: 3)
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PCR bands on gel electrophoresis - (reply: 5)
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Identify the band in western blot - (reply: 5)
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Ponceau staining - Appearence of bands (reply: 1)
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Multiple bands after amping insert - (reply: 2)
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Protein bands on the bottom of the gel - (reply: 5)
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Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
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Why is there extra band in IMAC SDS-PAGE - (reply: 1)
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Bands in a miRNA gel - (reply: 1)
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Gel Electrophoresis band problems - (reply: 3)
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Problem with protein samples - No bands detected at all on gel (reply: 2)
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Unhappy bands - (reply: 3)
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High MW PCR band seen...help needed - (reply: 7)
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Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands? (reply: 9)
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HELP! Genomic DNA, RNA-size bands - (reply: 2)
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No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
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"Diffuesed" bands of higher molecular weight - (reply: 6)
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Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
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strange band in coomassie blue staining - (reply: 3)
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nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
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Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
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why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
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Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
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PCR troubleshooting - two band after agrose electophoresis (reply: 4)
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Wrong bands on the blot - (reply: 4)
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Problems detecting my band! - (reply: 2)
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Western bands fading out, pleeeese help - (reply: 1)
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western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
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Why do I always get these ugly bands? - I always end up with some fused and tandemed bands... (reply: 7)
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Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
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Bands visible in membrane but not in the film - ?? (reply: 10)
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non desired brigther bands - (reply: 7)
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Bands on adjescent lanes!!! - wierd!!! (reply: 11)
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Only DNA ladder , No desired band in PCR - (reply: 4)
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Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
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Lost a Band by changing thermocycler - (reply: 9)
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problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
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Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
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how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
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Confusing bands from PCR - not primer dimer, not product (reply: 30)
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Bands appearing out of no where?! - (reply: 5)
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EXtraction of DNA bands from acrylamide gel - (reply: 4)
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High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
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Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
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casting agarose gel troubleshooting - smeared bands (reply: 8)
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How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
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Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
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the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
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overloading GAPDH but nice band of interest - (reply: 2)
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Quantiying bands. - Determination of protein contens (reply: 2)
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after re-isolation of vector, get single band - (reply: 4)
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BSP PCR for help - no bands of tissue amplification (reply: 3)
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2 bands or smear? 2-step RT-PCR - (reply: 1)
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Too many bands in my SDS-PAGE - (reply: 5)
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Uneven bands in SDS-PAGE - (reply: 5)
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weak bands for methylated DNA - (reply: 4)
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Two bands for p53 on Western Blot - (reply: 7)
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nonspecific bands after co-ip - (reply: 2)
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No PCR bands with some templates - (reply: 5)
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EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
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Blurred low molecular weight bands in SDS-PAGE - Need your help (reply: 1)
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Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
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uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
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How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
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light bands, dark smear, dark dimers - (reply: 2)
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pcr doesnt show any band for DNa walking - (reply: 8)
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Immunoprecipitation - Bands in negative control - (reply: 2)
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crappy bands after developing - (reply: 3)
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Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
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no bands in my PCR - (reply: 9)
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All gradient samples give bands! How come? - (reply: 4)
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many bands of smaller size than expected - (reply: 3)
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wrong bands after T-vector cloning - (reply: 3)
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abnormal band in agarose gel - Urgent help required (reply: 3)
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Ghost bands in western blot - Western blotting problems (reply: 9)
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PCR contamination with human DNA first and now no bands!!! - (reply: 4)
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Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
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non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
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Non-specific bands on EMSA - (reply: 2)
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Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
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Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
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PCR ghost bands - (reply: 1)
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Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
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strange extra bands on digest of miniprep? - (reply: 5)
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GST preotin non specific preotins - Non specific GST bands (reply: 2)
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3'RACE and the polyA tail - why only one band? (reply: 2)
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higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
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A single band on WB smaller than expected - (reply: 3)
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Low sized bands in plasmid preperations - its not RNA! (reply: 13)
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Co-IP problem - Weird IgG band (reply: 1)
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Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
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EMSA supershift - increased band intensity? - (reply: 1)
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DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
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no band in gel - gel electrophoresis (reply: 1)
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SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
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Strange bands in western of RPE-1 cells - (reply: 1)
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no 18S band on RNA agarose gel - (reply: 1)
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Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
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multiple bands in my RNA sample? - (reply: 2)
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multiple bands in my RNA sample? - (reply: 2)
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Ghost band in SDS PAGE - (reply: 2)
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No band in the gel after ligation - (reply: 6)
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Unidentified DNA bands - (reply: 1)
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silica purification - problem with double bands after silica purification (reply: 1)
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PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
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normal PCR - two bands is found in one sample (reply: 3)
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Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
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After double digestion, one weird band on the very top! - (reply: 4)
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After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
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Immunoprecipitation (optimization?) problem - anti-ovalbumin lane has 225 kDa band. (reply: 7)
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strange double band in electrophoretic pattern - (reply: 2)
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protein band size determination - any software available? (reply: 2)