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Band Related Discussions
  1. Multiple bands in eluted PCR product - (reply: 1)
  2. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 3)
  3. BAC miniprep-high nanodrop value but NO BAND - (reply: 2)
  4. multiple bands for genomic DNA extraction - (reply: 2)
  5. The same strong bands with different primary antibody - (reply: 1)
  6. many bands Western Blot - (reply: 2)
  7. unespecific band PCR from mouse genomic DNA - (reply: 9)
  8. Help with unknown band in restriction digest - (reply: 1)
  9. Preference for larger of 2 bands? - (reply: 2)
  10. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  11. RNase Band in old plasmid stored at -40 - (reply: 2)
  12. getting rid of non specific bands in IP experiments - (reply: 7)
  13. strange bands shape agarose gel - (reply: 2)
  14. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
  15. Getting too Many Bands on my Western Blot - (reply: 9)
  16. Extra bands after protein expression/purification - (reply: 4)
  17. sequencing result from excised DGGE bands - (reply: 3)
  18. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
  19. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  20. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  21. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  22. Analyzing Western Bands by Densiometry - (reply: 2)
  23. Problem: Cloning DNA shuffled band - (reply: 2)
  24. Problems with a PCR-No bands and I am starting to question my sanity - (reply: 4)
  25. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
  26. Problem with PCR - large extra bands - (reply: 3)
  27. Same non specific band for different target proteins - (reply: 1)
  28. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
  29. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
  30. No band in PCR - (reply: 5)
  31. Identically loaded western bands loose intensity from left to right - (reply: 3)
  32. smear bands in western blot - (reply: 12)
  33. bands fuse together - (reply: 1)
  34. Measuring of cDNA bands density - (reply: 3)
  35. No band after extracting DNA from bacillus subtilis - (reply: 1)
  36. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  37. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
  38. Re-using membranes with ghost bands - (reply: 2)
  39. huge dot appears at the centre of every of my bands - (reply: 2)
  40. Bright bands of PCR products stick in gel wells - (reply: 1)
  41. Separating close bands in Western Blot - (reply: 3)
  42. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  43. Bands in my PCR Controls - (reply: 3)
  44. Strange band in colony PCR - (reply: 2)
  45. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  46. Bands seen with qPCR missing in regular PCR - (reply: 5)
  47. non specific bands - (reply: 7)
  48. why cant get the band? - (reply: 4)
  49. Western Blot no target bands, show Non-specific bands - (reply: 5)
  50. Bands disappear after longer exposure - (reply: 4)
  51. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  52. How to remove the background and unspecific bands - (reply: 12)
  53. Same Non-specific bands show up for different Abs!! - (reply: 7)
  54. what caused bubble band of my WB? - (reply: 4)
  55. Strange bands and large smear in western blot - (reply: 4)
  56. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
  57. HELP!- strange band appearing on immunoblot - strange band detected during blotting with anti-CTB antibodies (reply: 3)
  58. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  59. Band Quantitation - Bio-Rad's Quantity One (reply: 1)
  60. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
  61. how to get rid of non specific bands in PCR - (reply: 1)
  62. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
  63. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
  64. SDS PAGE smearing of bands - (reply: 2)
  65. Fuzzy bands in western blot - (reply: 2)
  66. Large Non-specfic bands in PCR - (reply: 3)
  67. HELP!! I ruin my Native-PAGE (newbie in PAGE) - My bands seem faint smear (reply: 3)
  68. 2 bands in blue colony PCR - (reply: 5)
  69. no bands after pcr - even after we have change most of the reagents (reply: 2)
  70. Identify ponceau visible bands - (reply: 1)
  71. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  72. Many unspecific bands after using anti-His on Western blot - (reply: 4)
  73. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  74. KO mice show band for knocked-out protein - (reply: 1)
  75. western blot bands wider at the middle than the top - (reply: 2)
  76. PCR Gel nonspecific band - (reply: 13)
  77. can't get bands in Western Blot - (reply: 5)
  78. difference in band sizes ! - why ??? (reply: 2)
  79. Funny DNA bands on Agarose gels - (reply: 6)
  80. Actin Bands smiling on western blot - (reply: 3)
  81. PCR: always got band in the negative control - (reply: 8)
  82. nonspecific bands on film - (reply: 3)
  83. 'lace-like texture' on my northern blot bands - (reply: 8)
  84. Problem Viewing bands in Agarose Gel - (reply: 6)
  85. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  86. software for quantifing PCR product band - (reply: 1)
  87. Multiple bands from purified PCR product - (reply: 3)
  88. Quantification of proteins in Western Bands - (reply: 2)
  89. No bands on agarose gel after restriction digestion. - (reply: 10)
  90. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  91. Extra band on gel after miniprep - (reply: 6)
  92. 3 distinct bands of gDNA on gel - (reply: 2)
  93. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  94. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  95. Total RNA only produce 1 band in gel - (reply: 2)
  96. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 3)
  97. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
  98. weird bands after minipreps - molecular cloning (reply: 2)
  99. Band at same position using different primary antibodies - (reply: 3)
  100. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  101. PCR, using gel extracted band as a template - (reply: 4)
  102. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  103. PCR bands in NTC but NOT in negative samples - (reply: 2)
  104. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
  105. Unusual band in transformants - (reply: 2)
  106. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  107. Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
  108. no band, extremely clean blot - (reply: 1)
  109. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  110. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  111. IgG - unspecific band (reply: 3)
  112. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
  113. "Unzipped" PCR band - (reply: 3)
  114. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  115. uncut vector contamination in gel purified band - (reply: 2)
  116. I see bands with western blot only the third day! - (reply: 2)
  117. incorrect/smaller band size for real time PCR - (reply: 1)
  118. Agarose gel bands smear/ drag problem - (reply: 4)
  119. Northern - Do not get Bands in Northern Blot (reply: 2)
  120. two bands detected in western - (reply: 1)
  121. Protein SDS-PAGE thick bands / smear - (reply: 4)
  122. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  123. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
  124. need help plz, band sizes estimation - (reply: 2)
  125. What causes this offset band on RIPA gel? - (reply: 4)
  126. nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
  127. band diffusion in sds - (reply: 5)
  128. too much bands in western - (reply: 2)
  129. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  130. Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 3)
  131. Bands are lower than actual protein - (reply: 1)
  132. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  133. No band after WB revelation - (reply: 2)
  134. No band after digestion - (reply: 4)
  135. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
  136. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  137. PCR -No band formation - (reply: 1)
  138. PCR - No Band formation - (reply: 3)
  139. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
  140. 2d page - no band (reply: 2)
  141. never get bands by pfu related enzymes - (reply: 2)
  142. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  143. Lower MW bands than expected. - (reply: 1)
  144. Multiple bands after bisulfite PCR - (reply: 7)
  145. western bands look funny - (reply: 5)
  146. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  147. quantifying "ghost" bands - (reply: 1)
  148. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
  149. Extra bands after QIAGEN spin miniprep - (reply: 3)
  150. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  151. Cold probe doesnt compete away bands - (reply: 2)
  152. Strength of actin signal - How dark should the band be? (reply: 5)
  153. Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
  154. PCR product appear two close band in my gel - (reply: 15)
  155. The Case of a Missing Band: PCR Issue - (reply: 4)
  156. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  157. Band appear and disapper in the negative control ?? - (reply: 2)
  158. Bands in PCR negative control - (reply: 3)
  159. Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
  160. western exposure one part of band darker - (reply: 6)
  161. non-specific bands - (reply: 5)
  162. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  163. bands of protein seems like it did not move - (reply: 2)
  164. downshifted band - (reply: 4)
  165. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
  166. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
  167. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  168. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  169. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
  170. more than one band - (reply: 1)
  171. IGg bands on SDS PAGE - (reply: 2)
  172. PCR band too thick - PCR troubleshooting question (reply: 5)
  173. ugly merged bands in western blot - (reply: 3)
  174. PCR bands on gel electrophoresis - (reply: 5)
  175. Identify the band in western blot - (reply: 5)
  176. Ponceau staining - Appearence of bands (reply: 1)
  177. Multiple bands after amping insert - (reply: 2)
  178. Protein bands on the bottom of the gel - (reply: 5)
  179. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
  180. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
  181. Bands in a miRNA gel - (reply: 1)
  182. Gel Electrophoresis band problems - (reply: 3)
  183. Problem with protein samples - No bands detected at all on gel (reply: 2)
  184. Unhappy bands - (reply: 3)
  185. High MW PCR band seen...help needed - (reply: 7)
  186. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands？ (reply: 9)
  187. HELP! Genomic DNA, RNA-size bands - (reply: 2)
  188. No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
  189. "Diffuesed" bands of higher molecular weight - (reply: 6)
  190. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  191. strange band in coomassie blue staining - (reply: 3)
  192. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  193. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  194. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  195. Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
  196. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  197. Wrong bands on the blot - (reply: 4)
  198. Problems detecting my band! - (reply: 2)
  199. Western bands fading out, pleeeese help - (reply: 1)
  200. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
  201. Why do I always get these ugly bands？ - I always end up with some fused and tandemed bands... (reply: 7)
  202. Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
  203. Bands visible in membrane but not in the film - ?? (reply: 10)
  204. non desired brigther bands - (reply: 7)
  205. Bands on adjescent lanes!!! - wierd!!! (reply: 11)
  206. Only DNA ladder , No desired band in PCR - (reply: 4)
  207. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  208. Lost a Band by changing thermocycler - (reply: 9)
  209. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
  210. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  211. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  212. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  213. Bands appearing out of no where?! - (reply: 5)
  214. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  215. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
  216. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  217. casting agarose gel troubleshooting - smeared bands (reply: 8)
  218. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  219. Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
  220. the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
  221. overloading GAPDH but nice band of interest - (reply: 2)
  222. Quantiying bands. - Determination of protein contens (reply: 2)
  223. after re-isolation of vector, get single band - (reply: 4)
  224. BSP PCR for help - no bands of tissue amplification (reply: 3)
  225. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  226. Too many bands in my SDS-PAGE - (reply: 5)
  227. Uneven bands in SDS-PAGE - (reply: 5)
  228. weak bands for methylated DNA - (reply: 4)
  229. Two bands for p53 on Western Blot - (reply: 7)
  230. nonspecific bands after co-ip - (reply: 2)
  231. No PCR bands with some templates - (reply: 5)
  232. EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
  233. Blurred low molecular weight bands in SDS-PAGE - Need your help (reply: 1)
  234. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
  235. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
  236. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
  237. light bands, dark smear, dark dimers - (reply: 2)
  238. pcr doesnt show any band for DNa walking - (reply: 8)
  239. Immunoprecipitation - Bands in negative control - (reply: 2)
  240. crappy bands after developing - (reply: 3)
  241. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  242. no bands in my PCR - (reply: 9)
  243. All gradient samples give bands! How come? - (reply: 4)
  244. many bands of smaller size than expected - (reply: 3)
  245. wrong bands after T-vector cloning - (reply: 3)
  246. abnormal band in agarose gel - Urgent help required (reply: 3)
  247. Ghost bands in western blot - Western blotting problems (reply: 9)
  248. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  249. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  250. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
  251. Non-specific bands on EMSA - (reply: 2)
  252. Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
  253. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  254. PCR ghost bands - (reply: 1)
  255. Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
  256. strange extra bands on digest of miniprep? - (reply: 5)
  257. GST preotin non specific preotins - Non specific GST bands (reply: 2)
  258. 3'RACE and the polyA tail - why only one band? (reply: 2)
  259. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
  260. A single band on WB smaller than expected - (reply: 3)
  261. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  262. Co-IP problem - Weird IgG band (reply: 1)
  263. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
  264. EMSA supershift - increased band intensity? - (reply: 1)
  265. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  266. no band in gel - gel electrophoresis (reply: 1)
  267. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
  268. Strange bands in western of RPE-1 cells - (reply: 1)
  269. no 18S band on RNA agarose gel - (reply: 1)
  270. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  271. multiple bands in my RNA sample? - (reply: 2)
  272. multiple bands in my RNA sample? - (reply: 2)
  273. Ghost band in SDS PAGE - (reply: 2)
  274. No band in the gel after ligation - (reply: 6)
  275. Unidentified DNA bands - (reply: 1)
  276. silica purification - problem with double bands after silica purification (reply: 1)
  277. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  278. normal PCR - two bands is found in one sample (reply: 3)
  279. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  280. After double digestion, one weird band on the very top! - (reply: 4)
  281. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  282. Immunoprecipitation (optimization?) problem - anti-ovalbumin lane has 225 kDa band. (reply: 7)
  283. strange double band in electrophoretic pattern - (reply: 2)
  284. protein band size determination - any software available? (reply: 2)