aditional band on WB - (Oct/03/2012 )
I am using a coctail of antibodies from cell signal: http://www.cellsignal.com/products/5301.html
On the WB photo given on their web site you can see a right above the Erk1/2 badn a faint nonspecific band just below 50kD. In my pituitary adenoma samples this band is extremely strong. Do you have any suggestion where this come from? I expect protein degradation to result in non-specific bands of lower molecular weight.
Thank you in advance for your answers.
Many antibodies give non-specific bands, these can vary depending on the tissue, the conditions that you used to for the protein extraction, and the detection conditions.
If you really want to know which antibody the band is from, you will probably have to get the individual antibodies and do peptide competition experiments to determine specificity.