Multiple Non-Specific Bands in Western Blot Detected... - (Feb/13/2013 )
Hi All !!!!
I recently did WB of total histones from Chicken Liver samples with Anti-Histone H3 antibody from Abcam ( #ab1791 here is the link to its details : http://www.abcam.com/Histone-H3-antibody-ChIP-Grade-ab1791.html ) used at dilution 1:70,000 .
Now the problem is that it picks multiple non-specific bands.
The typical histone profile of chicken looks like
but you can see in my blots (as attached file) that in addition to picking H3 and delta-H3, it non-specifically picks H1 and probably H5 even at 1:70,000 dilution. Also, a band just above H3 which usually is not visible in gel is also detected by it (?????).
So, could anybody pls help me with that !!!
don't worry about H1 and H5. your delta-H3 looks pretty sharp.