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Re-using membranes with ghost bands - (Oct/26/2011 )

My question is if I can reblot a membrane with ghost bands again with the same antibody but less concentrate. Is this possible or I have to repeat the gel?
Attached Image

-Irene G-

The Abs don't wash away. You would have to strip the membrane and then reprobe, but this is not very reproducible.


Thank you, I will make a new gel!

-Irene G-