huge dot appears at the centre of every of my bands - (Oct/17/2011 )
It looks to me like you've pierced your well with the end of the pipette tip while loading the sample creating a funny shape in your bands. Next time try no to put the tip so deep in the well.
just my 2 cents!
oh I see. I think that must be problem.I recalled that I wanted to make sure that the DNA I was loading does not escape too much out of the well so i purposely place the tip slightly deeper(not pierce the bottom of well though). Thankx alot:)