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are they non-specific band? - (May/25/2013 )

I am student and I did a PCR. The expected band size is 181bp. And I found that there is a fade band below my expected band. Are they non-specific bands? or degradation? Thx

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-man121-

The lower bands appear to me to be specific bands, they could be an isoform of the gene. What kind of PCR (gDNA or cDNA) are you doing?

-pcrman-

It is gDNA, how could I avoid it?

-man121-

So what have you done to optimise the reaction?

-bob1-

It might be worth checking the melting plots to confirm its a specific band; there will likely be a second smaller peak near the main peak.

-JoeSus-