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Real-time PCR Related Discussions
  1. Possible to change SimpleProbe/Melting curve to TaqMan or Scorpion? - (reply: 1)
  2. QPCR with genomic DNA from blood - (reply: 2)
  3. qPCR data analysis - (reply: 1)
  4. SYBR Green Real time PCR - (reply: 2)
  5. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
  6. Real-time pcr - (reply: 1)
  7. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
  8. QPCR replicates issue - (reply: 1)
  9. qPCR data analysis from raw data - (reply: 1)
  10. Primer calculation for qPCR - final reaction - (reply: 1)
  11. Help with definitons for qPCR - (reply: 1)
  12. Calibrator in qPCR - (reply: 7)
  13. standards for qPCR and some other doubts - (reply: 9)
  14. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
  15. qPCR with different genes - (reply: 5)
  16. qPCR normalization - (reply: 4)
  17. qPCR acceptable standard deviation - (reply: 1)
  18. qPCR standard for lipase genes - (reply: 2)
  19. Campylobacter jejuni qPCR targeting VS1 gene - (reply: 2)
  20. Newbie to qPCR - (reply: 4)
  21. real time qPCR standard range plasmid - (reply: 3)
  22. t-test & qRT-PCR - (reply: 1)
  23. Absolute quant with TaqMan - Different RT & different plates? - (reply: 1)
  24. Question about baselines in Real-Time PCR - (reply: 3)
  25. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 2)
  26. TaqMan gene expression assays - (reply: 1)
  27. labX offers on realtime PCR machines - (reply: 4)
  28. qPCR error bars - confused! - (reply: 2)
  29. qPCR uniformity test - (reply: 1)
  30. need help setting up reverse transcriptase qPCR - (reply: 3)
  31. PCR working, qPCR is *not*. - (reply: 6)
  32. No signal in qPCR from ChIP sample - (reply: 1)
  33. When to dilute samples for qPCR - (reply: 2)
  34. help with qPCR - (reply: 1)
  35. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  36. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  37. Troubleshooting qPCR standards - (reply: 1)
  38. qPCR , H&E, IHC for Influenza is not correlated - (reply: 3)
  39. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  40. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  41. Taqman probe in a LightCycler 480? - (reply: 1)
  42. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  43. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  44. qPCR and miRNA isolation - (reply: 4)
  45. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
  46. Reference gene(s) selection and validaton for qPCR - (reply: 3)
  47. Is it possible quantify dsDNA pre-sequencing with a kit in stepone plus qpcr - (reply: 2)
  48. qPCR cannot detect reaction. - (reply: 1)
  49. qRT-PCR works one day, and not the next - (reply: 1)
  50. Inter run calibration qRT-PCR - (reply: 2)
  51. qPCR amplification - (reply: 4)
  52. Sybr Green Gold EtBr mechanism - (reply: 1)
  53. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
  54. how to hasten real-time PCR amplifications - (reply: 2)
  55. Different primer concentration in qPCR - (reply: 1)
  56. Problem with Real-time PCR results analysis - (reply: 1)
  57. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  58. polyacrylamide gel with sybr green staining - (reply: 1)
  59. Defining standards in qPCR softwares - (reply: 3)
  60. Having problem with primers for qPCR - (reply: 4)
  61. timecourse experiment for Western and qPCR - (reply: 2)
  62. suitable single-copy gene target for fungal qPCR - (reply: 4)
  63. qPCR of Intronless gene - (reply: 4)
  64. qPCR reference gene testing help - (reply: 4)
  65. ABI 7300 qRT-PCR and ddCT protocol - (reply: 1)
  66. excess rna sample affect qpcr? - (reply: 2)
  67. qpcr and sample dilution - (reply: 11)
  68. qPCR result of pooled samples - (reply: 1)
  69. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
  70. qPCR - (reply: 1)
  71. defining ratio of alternative spliced gene with qPCR - (reply: 14)
  72. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
  73. real-time pcr non reproducible - (reply: 4)
  74. Inexplicable qPCR failure in single wells - (reply: 12)
  75. analysing qpcr data and plotting standard curve - (reply: 1)
  76. TaqMan CN assay - control sample duplicated - (reply: 1)
  77. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  78. Basic question about qPCR and endogenous RNA control. - (reply: 4)
  79. CT of qPCR - (reply: 3)
  80. Sybr Green vs Taqman -- a practical approach - (reply: 2)
  81. cDNA amount in qPCR - (reply: 2)
  82. mRNA integrity for qPCR - (reply: 6)
  83. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
  84. Real-time efficiency question - (reply: 1)
  85. Selective removal of standards post qPCR Run - (reply: 1)
  86. qPCR question (no results?) - (reply: 1)
  87. >100% input in histone ChIP qPCR - (reply: 2)
  88. Sensitivity of RT-PCR and qPCR - (reply: 4)
  89. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  90. Are these primer products good enough for qPCR? - (reply: 3)
  91. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
  92. Taqman Probe sequence problem - (reply: 4)
  93. McrBC + qPCR : mock has higher Cts than digested? - (reply: 1)
  94. urgent: need help with qPCR statistics - (reply: 2)
  95. Dye for qPCR - (reply: 3)
  96. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  97. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
  98. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
  99. reference gene for qPCR in E.coli - (reply: 2)
  100. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
  101. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  102. qPCR detection - (reply: 2)
  103. qPCR with Genomic DNA - (reply: 1)
  104. How useful are Service Contracts for qPCR machines? - (reply: 2)
  105. Normalizing qPCR data - (reply: 1)
  106. good 16S univ. primer for qPCR? - (reply: 3)
  107. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
  108. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  109. thermocycler - real-time versus conventional? - (reply: 2)
  110. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
  111. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
  112. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  113. Multiple Bands in qPCR - (reply: 4)
  114. Is it possible to compare abundance of isoforms by qPCR - (reply: 6)
  115. Reference RNA for qPCR - (reply: 3)
  116. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  117. qRT-PCR data analysis - (reply: 2)
  118. Strange RT to qPCR results with new kit - (reply: 5)
  119. qPCR: band in gel, but super high Cq? - (reply: 10)
  120. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  121. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
  122. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  123. qPCR: CT value of reference gene - (reply: 3)
  124. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  125. Making a qPCR standard amplicon from cloning, why? - (reply: 4)
  126. geNorm alternative for qPCR housekeeping genes - (reply: 1)
  127. qPCR contamination problem - (reply: 5)
  128. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  129. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
  130. same primers, different product in conventional PCR and qPCR - (reply: 6)
  131. Plateau height qPCR - (reply: 3)
  132. UDG in qPCR - (reply: 1)
  133. Taqman Probes problem - (reply: 3)
  134. qPCR : why substracting CT values instead of dividing ? - (reply: 3)
  135. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
  136. Problem/qPCR, 2 peaks en melting curve - (reply: 4)
  137. Recommended tamplate DNA concentration for qPCR - (reply: 2)
  138. Enough checks for gDNA contamination in qPCR? - (reply: 3)
  139. optical qPCR plates - (reply: 1)
  140. PCR optimization: PCR vs qPCR - (reply: 4)
  141. TaqMan qPCR low-template issues - (reply: 1)
  142. qPCR standard curve slope (m) - (reply: 2)
  143. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  144. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  145. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  146. Message Amount in qPCR - (reply: 1)
  147. cDNA concentration of qPCR - (reply: 1)
  148. qPCR normalization of reference gene - (reply: 1)
  149. Desperate! qPCR standard curve problems - (reply: 2)
  150. Home made real-time PCR problem - (reply: 4)
  151. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  152. Which Brand of real-time PCR machine - (reply: 6)
  153. Weird qPCR curves - (reply: 11)
  154. Problem with New qPCR primers - help! - (reply: 6)
  155. Validation of microarray data by qPCR - (reply: 1)
  156. Help with high Ct values for Real-Time PCR - (reply: 1)
  157. qRTPCR using taqman - (reply: 4)
  158. Advantage of sybr green over ethidium bromide - (reply: 1)
  159. qPCR analysis- can I use RQ? - (reply: 1)
  160. Error bars for qPCR; technical vs. biological replicate - (reply: 2)
  161. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  162. Too high CT values with TaqMan - (reply: 1)
  163. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
  164. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
  165. Real-Time efficiencies and the pfaffl method - (reply: 1)
  166. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  167. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
  168. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  169. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  170. qPCR Calc Help - (reply: 6)
  171. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
  172. false positive/cross reaction with TaqMan - (reply: 1)
  173. real time qRT-PCR and low expression gene - (reply: 2)
  174. Validating MeDIP by qPCR - (reply: 3)
  175. Bands seen with qPCR missing in regular PCR - (reply: 5)
  176. How to explain the difference between microarray results and qPCR data - (reply: 1)
  177. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  178. TaqMan Low density Array Card Analysis - (reply: 1)
  179. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  180. Weird qPCR curves - (reply: 4)
  181. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  182. real-time PCR after restriction enzyme digestion - (reply: 6)
  183. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  184. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
  185. Help! Smear in Real-Time PCR Product - (reply: 1)
  186. Sybr green RT-qPCR primer - (reply: 1)
  187. Sybr green RT-qPCR primer - (reply: 1)
  188. running multiple qPCR with common housekeeping control - (reply: 3)
  189. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  190. Consistancy in qPCR data - (reply: 5)
  191. qPCR with low expressing genes - (reply: 1)
  192. Calculating geometric mean for real-time PCR - (reply: 5)
  193. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
  194. >100% qPCR efficiency - (reply: 2)
  195. Improving qRT-PCR detection limits - (reply: 2)
  196. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  197. excel and propagation of error, qpcr - (reply: 1)
  198. Triage of miRNA targets by qPCR - (reply: 1)
  199. qPCR primer design - possible off-target (reply: 1)
  200. Primer dimer formation and real-time PCR - (reply: 7)
  201. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
  202. Sequencing miRNA qRT-PCR Products - (reply: 5)
  203. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
  204. Using DNA as a reference in qRT-PCR - (reply: 1)
  205. minimum replicate for qpcr? - (reply: 15)
  206. qPCR amplification interference fixed with freezing - (reply: 6)
  207. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
  208. qPCR volume - Problem with volume difference and results (reply: 1)
  209. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
  210. Effect of collagenase on qpcr results - (reply: 1)
  211. qPCR inhibition - (reply: 1)
  212. Primers for qPCR - (reply: 3)
  213. can RNA amplify in two-step qPCR? - (reply: 2)
  214. qPCR problem with miRNA - No signal in the qPCR (reply: 4)
  215. Using Sybr green in realtime pcr - (reply: 1)
  216. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
  217. how to determine the amount of cDNA for qPCR - (reply: 5)
  218. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
  219. standard curve for qPCR - (reply: 3)
  220. Standard curve for qPCR - (reply: 1)
  221. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
  222. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
  223. weird QPCR curves - (reply: 2)
  224. Denaturing gel electrophoresis for qPCR - (reply: 3)
  225. Long-amplicon QPCR of mtDNA - (reply: 5)
  226. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
  227. Help with real-time PCR quantification of miRNA - (reply: 8)
  228. negative control in qRT-PCR - (reply: 6)
  229. Question about RealTime PCR Primer Design - (reply: 1)
  230. How to present and analyze these real-time PCR data? - (reply: 2)
  231. qPCR for transformed cells - (reply: 1)
  232. Clinical Real-time PCR assay - (reply: 1)
  233. Standard curve for real-time PCR - (reply: 2)
  234. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  235. Real-time qPCR data analysis - (reply: 1)
  236. Is it possible to compare data between real-time PCR plates - (reply: 1)
  237. Questions of Standards making in relative qRT-PCR - (reply: 2)
  238. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  239. Reference genes and dilutions in qPCR - (reply: 1)
  240. Issue with standard curve for qPCR for CHiP - (reply: 1)
  241. qPCR data analysis - (reply: 1)
  242. qPCR Data analysis - qBasePlus and GenEx (reply: 1)
  243. degenerated Taqman probes? - (reply: 1)
  244. error message on qPCR mashine - (reply: 1)
  245. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  246. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  247. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  248. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
  249. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
  250. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  251. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
  252. qPCR Data Analysis - Software (reply: 3)
  253. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
  254. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  255. Primer design for qPCR - (reply: 2)
  256. Primer design for qPCR - (reply: 1)
  257. reporting results of qPCR - (reply: 1)
  258. question about qRT-PCR preparation - (reply: 1)
  259. qPCR efficiency calculation - (reply: 1)
  260. qPCR on chIP product - (reply: 3)
  261. how to save sybr green reagents? - (reply: 3)
  262. QPCR/DDCT Method/ Statistics - (reply: 2)
  263. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
  264. microRNA profiling using ABI taqman low density microRNA array (TLDA) - (reply: 1)
  265. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  266. Having abnormal dissociation curve when performing qPCR - (reply: 3)
  267. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  268. How much template DNA do you use for qPCR - (reply: 1)
  269. paranormal qpcr amplification activity - melting curves (reply: 3)
  270. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
  271. General qRT-PCR question - (reply: 3)
  272. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
  273. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
  274. qPCR absolute/relative quantification method question - (reply: 4)
  275. Compare different qPCR runs - really need help (reply: 8)
  276. qPCR of BS DNA - (reply: 6)
  277. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  278. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  279. standards for absolute quantification qPCR - (reply: 1)
  280. qPCR, RT- Control - (reply: 2)
  281. Pre-amplification of template for qPCR - (reply: 1)
  282. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
  283. issue with taqman MGB probes - (reply: 2)
  284. conventional v real-time PCR applications - (reply: 1)
  285. different PCR primers for real-time and classic PCR - (reply: 3)
  286. qRT-PCR Inconsistency - (reply: 1)
  287. question qPCR data presentation? - (reply: 1)
  288. increasing qPCR reaction volume - Quick and simple question of qPCR reaction volume (reply: 2)
  289. detect miRNAs expression by qPCR - (reply: 2)
  290. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  291. BSA as qPCR enhancer - NEB or Sigma? (reply: 3)
  292. Reference for qPCR Chip? - (reply: 1)
  293. Workflow of qRT-PCR for siRNA - help please. (reply: 5)
  294. Taqman real-time PCR - Taqman and contamination (reply: 4)
  295. Data Analysis for Real-time PCR - (reply: 2)
  296. Replicate inconsistencies in qPCR - (reply: 6)
  297. Real-Time PCR - Gene expression (reply: 2)
  298. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
  299. multiplexing taqman miRNA reverse transcription - (reply: 2)
  300. Analyzing qPCR results - (reply: 1)
  301. QPCR - Primer and Probe question - QPCR (reply: 2)
  302. qRT-PCR Product Size - (reply: 5)
  303. 18s control in qPCR - (reply: 2)
  304. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  305. qrt-pcr and northern blot ,which way is better? - (reply: 4)
  306. microrna quantitation ---use northern blot or qrt-pcr? - (reply: 1)
  307. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
  308. qPCR analysis - (reply: 6)
  309. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
  310. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  311. calibrator - what is this in qPCR (reply: 1)
  312. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  313. SYBR Green and Ethidium Bromide in same gel - Just out of curiosity (reply: 2)
  314. Realtime PCR machine - (reply: 2)
  315. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  316. home made sybr green mix for qpcr - (reply: 2)
  317. reference genes for qPCR - (reply: 3)
  318. Fermentas SYBR Green vs. Qiagen SYBR Green - (reply: 2)
  319. qPCR using standard curve method - how to do correct setup? (reply: 3)
  320. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  321. Best time to isolate RNA for qPCR? - (reply: 1)
  322. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  323. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
  324. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
  325. Long real-time Amplicon - (reply: 2)
  326. qPCR - no signal detected but plenty of product on gel - (reply: 3)
  327. Amplify cDNA for qRT-PCR? - (reply: 6)
  328. QPCR problem! - (reply: 9)
  329. Reference gene qPCR - (reply: 4)
  330. qRT-PCR no of cycles?? - (reply: 6)
  331. Is my primer going to work for qRT-PCR? - (reply: 6)
  332. Sybr Green I in real-time PCR reaction - (reply: 3)
  333. Measuring global methylation by QPCR - (reply: 1)
  334. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  335. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
  336. qRTPCR (TaqMan) Data Representation - (reply: 1)
  337. Ncode qRT-PCR - (reply: 6)
  338. ABI Taqman Low density microRNA Array card - (reply: 3)
  339. RT-PCR - problems with TaqMan PCR (reply: 2)
  340. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
  341. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  342. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  343. Freezing primers+SYBR green - (reply: 2)
  344. differently behavioring replicates in real-time PCR - (reply: 1)
  345. RT qPCR Newbie, Weird Results - (reply: 1)
  346. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  347. qPCR and varying cDNA concentrations - (reply: 1)
  348. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
  349. qPCR and quantity of RNA - (reply: 2)
  350. How & where to prepare qPCR - (reply: 9)
  351. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
  352. Normalization of qpcr data - (reply: 1)
  353. Making standard dilution for qPCR - (reply: 1)
  354. Primers and Taqman Probes mixture question - (reply: 1)
  355. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  356. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  357. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  358. Interpreting QPCR DATA - (reply: 3)
  359. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  360. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  361. Sequencing of scarce real-time amplicons - (reply: 1)
  362. miRNA real time PCR by SYBR green methods - (reply: 1)
  363. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  364. Real-Time PCR as a Microplate Reader - (reply: 1)
  365. re-amplification in real-time PCR - (reply: 1)
  366. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  367. Ct fluctuations in qPCR - (reply: 3)
  368. qPCR efficiency - how to improve (reply: 4)
  369. qPCR primer design - (reply: 5)
  370. template concentration qPCR - (reply: 1)
  371. qRT-PCR Problems - (reply: 1)
  372. qRT-PCR with DMSO - (reply: 2)
  373. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  374. Problems with qRT-PCR - (reply: 3)
  375. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
  376. Understanding qPCR and gene expression changes - (reply: 10)
  377. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  378. Taqman rtPCR primer and probe design - (reply: 3)
  379. SYBR Green waste removal - (reply: 2)
  380. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  381. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  382. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  383. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  384. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  385. about real time qPCR - (reply: 1)
  386. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  387. SYBR Green miRNA analysis - (reply: 1)
  388. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
  389. Can DEPC-water interfere QPCR reaction? - (reply: 4)
  390. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)
  391. SYBR and TaqMan - (reply: 6)
  392. Need helps with my QPCR result - (reply: 1)
  393. cheap sybr green reagent - (reply: 2)
  394. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  395. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  396. qPCR quantification of ChIP DNA - (reply: 2)