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Real-time PCR Related Discussions
  1. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 3)
  2. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  3. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  4. Message Amount in qPCR - (reply: 1)
  5. cDNA concentration of qPCR - (reply: 1)
  6. qPCR normalization of reference gene - (reply: 1)
  7. Desperate! qPCR standard curve problems - (reply: 2)
  8. Home made real-time PCR problem - (reply: 3)
  9. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  10. Which Brand of real-time PCR machine - (reply: 6)
  11. Weird qPCR curves - (reply: 11)
  12. Problem with New qPCR primers - help! - (reply: 6)
  13. Validation of microarray data by qPCR - (reply: 1)
  14. Help with high Ct values for Real-Time PCR - (reply: 1)
  15. qRTPCR using taqman - (reply: 4)
  16. qPCR analysis- can I use RQ? - (reply: 1)
  17. Error bars for qPCR; technical vs. biological replicate - (reply: 2)
  18. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  19. Too high CT values with TaqMan - (reply: 1)
  20. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
  21. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
  22. Real-Time efficiencies and the pfaffl method - (reply: 1)
  23. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  24. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
  25. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  26. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  27. qPCR Calc Help - (reply: 6)
  28. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
  29. false positive/cross reaction with TaqMan - (reply: 1)
  30. real time qRT-PCR and low expression gene - (reply: 2)
  31. Validating MeDIP by qPCR - (reply: 3)
  32. Bands seen with qPCR missing in regular PCR - (reply: 5)
  33. How to explain the difference between microarray results and qPCR data - (reply: 1)
  34. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  35. TaqMan Low density Array Card Analysis - (reply: 1)
  36. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  37. Weird qPCR curves - (reply: 4)
  38. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  39. real-time PCR after restriction enzyme digestion - (reply: 6)
  40. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  41. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
  42. Help! Smear in Real-Time PCR Product - (reply: 1)
  43. running multiple qPCR with common housekeeping control - (reply: 3)
  44. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  45. Consistancy in qPCR data - (reply: 5)
  46. qPCR with low expressing genes - (reply: 1)
  47. Calculating geometric mean for real-time PCR - (reply: 5)
  48. >100% qPCR efficiency - (reply: 2)
  49. Improving qRT-PCR detection limits - (reply: 2)
  50. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  51. excel and propagation of error, qpcr - (reply: 1)
  52. Triage of miRNA targets by qPCR - (reply: 1)
  53. qPCR primer design - possible off-target (reply: 1)
  54. Primer dimer formation and real-time PCR - (reply: 7)
  55. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
  56. Sequencing miRNA qRT-PCR Products - (reply: 5)
  57. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
  58. Using DNA as a reference in qRT-PCR - (reply: 1)
  59. minimum replicate for qpcr? - (reply: 15)
  60. qPCR amplification interference fixed with freezing - (reply: 6)
  61. qPCR volume - Problem with volume difference and results (reply: 1)
  62. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
  63. Effect of collagenase on qpcr results - (reply: 1)
  64. qPCR inhibition - (reply: 1)
  65. Primers for qPCR - (reply: 3)
  66. can RNA amplify in two-step qPCR? - (reply: 2)
  67. qPCR problem with miRNA - No signal in the qPCR (reply: 4)
  68. Using Sybr green in realtime pcr - (reply: 1)
  69. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
  70. how to determine the amount of cDNA for qPCR - (reply: 5)
  71. standard curve for qPCR - (reply: 3)
  72. Standard curve for qPCR - (reply: 1)
  73. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
  74. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
  75. weird QPCR curves - (reply: 2)
  76. Denaturing gel electrophoresis for qPCR - (reply: 3)
  77. Long-amplicon QPCR of mtDNA - (reply: 5)
  78. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
  79. Help with real-time PCR quantification of miRNA - (reply: 8)
  80. negative control in qRT-PCR - (reply: 6)
  81. Question about RealTime PCR Primer Design - (reply: 1)
  82. How to present and analyze these real-time PCR data? - (reply: 2)
  83. qPCR for transformed cells - (reply: 1)
  84. Clinical Real-time PCR assay - (reply: 1)
  85. Standard curve for real-time PCR - (reply: 2)
  86. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  87. Real-time qPCR data analysis - (reply: 1)
  88. Is it possible to compare data between real-time PCR plates - (reply: 1)
  89. Questions of Standards making in relative qRT-PCR - (reply: 2)
  90. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  91. Reference genes and dilutions in qPCR - (reply: 1)
  92. Issue with standard curve for qPCR for CHiP - (reply: 1)
  93. qPCR data analysis - (reply: 1)
  94. qPCR Data analysis - qBasePlus and GenEx (reply: 1)
  95. degenerated Taqman probes? - (reply: 1)
  96. error message on qPCR mashine - (reply: 1)
  97. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  98. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 4)
  99. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  100. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
  101. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  102. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 33)
  103. qPCR Data Analysis - Software (reply: 3)
  104. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
  105. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  106. Primer design for qPCR - (reply: 2)
  107. Primer design for qPCR - (reply: 1)
  108. reporting results of qPCR - (reply: 1)
  109. question about qRT-PCR preparation - (reply: 1)
  110. qPCR efficiency calculation - (reply: 1)
  111. qPCR on chIP product - (reply: 3)
  112. QPCR/DDCT Method/ Statistics - (reply: 2)
  113. microRNA profiling using ABI taqman low density microRNA array (TLDA) - (reply: 1)
  114. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  115. Having abnormal dissociation curve when performing qPCR - (reply: 3)
  116. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  117. How much template DNA do you use for qPCR - (reply: 1)
  118. paranormal qpcr amplification activity - melting curves (reply: 3)
  119. General qRT-PCR question - (reply: 3)
  120. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
  121. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
  122. qPCR absolute/relative quantification method question - (reply: 4)
  123. Compare different qPCR runs - really need help (reply: 7)
  124. qPCR of BS DNA - (reply: 6)
  125. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  126. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  127. standards for absolute quantification qPCR - (reply: 1)
  128. qPCR, RT- Control - (reply: 2)
  129. Pre-amplification of template for qPCR - (reply: 1)
  130. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
  131. issue with taqman MGB probes - (reply: 2)
  132. conventional v real-time PCR applications - (reply: 1)
  133. different PCR primers for real-time and classic PCR - (reply: 3)
  134. qRT-PCR Inconsistency - (reply: 1)
  135. question qPCR data presentation? - (reply: 1)
  136. increasing qPCR reaction volume - Quick and simple question of qPCR reaction volume (reply: 2)
  137. detect miRNAs expression by qPCR - (reply: 2)
  138. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  139. BSA as qPCR enhancer - NEB or Sigma? (reply: 3)
  140. Reference for qPCR Chip? - (reply: 1)
  141. Workflow of qRT-PCR for siRNA - help please. (reply: 5)
  142. Taqman real-time PCR - Taqman and contamination (reply: 4)
  143. Data Analysis for Real-time PCR - (reply: 2)
  144. Replicate inconsistencies in qPCR - (reply: 6)
  145. Real-Time PCR - Gene expression (reply: 2)
  146. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
  147. multiplexing taqman miRNA reverse transcription - (reply: 2)
  148. Analyzing qPCR results - (reply: 1)
  149. QPCR - Primer and Probe question - QPCR (reply: 2)
  150. qRT-PCR Product Size - (reply: 5)
  151. 18s control in qPCR - (reply: 2)
  152. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  153. qrt-pcr and northern blot ,which way is better? - (reply: 4)
  154. microrna quantitation ---use northern blot or qrt-pcr？ - (reply: 1)
  155. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
  156. qPCR analysis - (reply: 6)
  157. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
  158. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  159. calibrator - what is this in qPCR (reply: 1)
  160. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  161. Realtime PCR machine - (reply: 2)
  162. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  163. home made sybr green mix for qpcr - (reply: 2)
  164. reference genes for qPCR - (reply: 3)
  165. qPCR using standard curve method - how to do correct setup? (reply: 3)
  166. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  167. Best time to isolate RNA for qPCR? - (reply: 1)
  168. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
  169. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
  170. Long real-time Amplicon - (reply: 2)
  171. qPCR - no signal detected but plenty of product on gel - (reply: 3)
  172. Amplify cDNA for qRT-PCR? - (reply: 4)
  173. QPCR problem! - (reply: 9)
  174. Reference gene qPCR - (reply: 4)
  175. qRT-PCR no of cycles?? - (reply: 6)
  176. Is my primer going to work for qRT-PCR? - (reply: 5)
  177. Sybr Green I in real-time PCR reaction - (reply: 3)
  178. Measuring global methylation by QPCR - (reply: 1)
  179. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  180. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
  181. qRTPCR (TaqMan) Data Representation - (reply: 1)
  182. Ncode qRT-PCR - (reply: 6)
  183. ABI Taqman Low density microRNA Array card - (reply: 3)
  184. RT-PCR - problems with TaqMan PCR (reply: 2)
  185. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
  186. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  187. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  188. differently behavioring replicates in real-time PCR - (reply: 1)
  189. RT qPCR Newbie, Weird Results - (reply: 1)
  190. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  191. qPCR and varying cDNA concentrations - (reply: 1)
  192. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
  193. qPCR and quantity of RNA - (reply: 2)
  194. How & where to prepare qPCR - (reply: 8)
  195. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
  196. Normalization of qpcr data - (reply: 1)
  197. Making standard dilution for qPCR - (reply: 1)
  198. Primers and Taqman Probes mixture question - (reply: 1)
  199. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  200. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  201. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  202. Interpreting QPCR DATA - (reply: 3)
  203. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  204. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  205. Sequencing of scarce real-time amplicons - (reply: 1)
  206. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  207. Real-Time PCR as a Microplate Reader - (reply: 1)
  208. re-amplification in real-time PCR - (reply: 1)
  209. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  210. Ct fluctuations in qPCR - (reply: 3)
  211. qPCR efficiency - how to improve (reply: 4)
  212. qPCR primer design - (reply: 5)
  213. template concentration qPCR - (reply: 1)
  214. qRT-PCR Problems - (reply: 1)
  215. qRT-PCR with DMSO - (reply: 2)
  216. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  217. Problems with qRT-PCR - (reply: 3)
  218. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
  219. Understanding qPCR and gene expression changes - (reply: 10)
  220. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  221. Taqman rtPCR primer and probe design - (reply: 3)
  222. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  223. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  224. Real-Time RT-PCR one-step and two step issue - (reply: 12)
  225. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  226. about real time qPCR - (reply: 1)
  227. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  228. What went wrong in my qPCR? - please look at the dissociation curve (reply: 24)
  229. Can DEPC-water interfere QPCR reaction? - (reply: 4)
  230. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)
  231. SYBR and TaqMan - (reply: 6)
  232. Need helps with my QPCR result - (reply: 1)
  233. Negative Control for ChIP realtime PCR in Mouse - (reply: 11)
  234. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  235. qPCR quantification of ChIP DNA - (reply: 2)