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Real-time PCR Related Discussions
  1. Pre-designed qRT-PCR primers - (reply: 1)
  2. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
  3. qPCR run w. strong positive NTC signal but very low fluorescence - (reply: 1)
  4. making your own qPCR kit! - (reply: 1)
  5. Problem with TaqMan qPCR efficiency - (reply: 1)
  6. relative quantification - things to do before the actual qPCR - (reply: 3)
  7. Strange Taqman probe negative fluorescence - (reply: 2)
  8. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
  9. qPCR , eDNA and standard curve - (reply: 3)
  10. Possible to change SimpleProbe/Melting curve to TaqMan or Scorpion? - (reply: 1)
  11. QPCR with genomic DNA from blood - (reply: 2)
  12. qPCR data analysis - (reply: 2)
  13. SYBR Green Real time PCR - (reply: 2)
  14. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
  15. Real-time pcr - (reply: 1)
  16. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
  17. QPCR replicates issue - (reply: 1)
  18. qPCR data analysis from raw data - (reply: 1)
  19. Primer calculation for qPCR - final reaction - (reply: 1)
  20. Help with definitons for qPCR - (reply: 1)
  21. Calibrator in qPCR - (reply: 7)
  22. standards for qPCR and some other doubts - (reply: 9)
  23. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
  24. qPCR with different genes - (reply: 5)
  25. qPCR normalization - (reply: 4)
  26. qPCR acceptable standard deviation - (reply: 1)
  27. qPCR standard for lipase genes - (reply: 2)
  28. Campylobacter jejuni qPCR targeting VS1 gene - (reply: 2)
  29. Newbie to qPCR - (reply: 4)
  30. real time qPCR standard range plasmid - (reply: 3)
  31. t-test & qRT-PCR - (reply: 1)
  32. Absolute quant with TaqMan - Different RT & different plates? - (reply: 1)
  33. Question about baselines in Real-Time PCR - (reply: 3)
  34. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 2)
  35. TaqMan gene expression assays - (reply: 1)
  36. labX offers on realtime PCR machines - (reply: 4)
  37. qPCR error bars - confused! - (reply: 2)
  38. qPCR uniformity test - (reply: 1)
  39. need help setting up reverse transcriptase qPCR - (reply: 3)
  40. PCR working, qPCR is *not*. - (reply: 6)
  41. No signal in qPCR from ChIP sample - (reply: 1)
  42. When to dilute samples for qPCR - (reply: 2)
  43. help with qPCR - (reply: 1)
  44. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  45. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  46. Troubleshooting qPCR standards - (reply: 1)
  47. qPCR , H&E, IHC for Influenza is not correlated - (reply: 3)
  48. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  49. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  50. Taqman probe in a LightCycler 480? - (reply: 1)
  51. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  52. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  53. qPCR and miRNA isolation - (reply: 4)
  54. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
  55. Reference gene(s) selection and validaton for qPCR - (reply: 5)
  56. Is it possible quantify dsDNA pre-sequencing with a kit in stepone plus qpcr - (reply: 2)
  57. qPCR cannot detect reaction. - (reply: 1)
  58. qRT-PCR works one day, and not the next - (reply: 1)
  59. Inter run calibration qRT-PCR - (reply: 2)
  60. qPCR amplification - (reply: 4)
  61. Sybr Green Gold EtBr mechanism - (reply: 1)
  62. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
  63. how to hasten real-time PCR amplifications - (reply: 2)
  64. Different primer concentration in qPCR - (reply: 1)
  65. Problem with Real-time PCR results analysis - (reply: 1)
  66. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  67. polyacrylamide gel with sybr green staining - (reply: 1)
  68. Defining standards in qPCR softwares - (reply: 3)
  69. Having problem with primers for qPCR - (reply: 4)
  70. timecourse experiment for Western and qPCR - (reply: 2)
  71. suitable single-copy gene target for fungal qPCR - (reply: 4)
  72. qPCR of Intronless gene - (reply: 4)
  73. qPCR reference gene testing help - (reply: 4)
  74. ABI 7300 qRT-PCR and ddCT protocol - (reply: 1)
  75. excess rna sample affect qpcr? - (reply: 2)
  76. qpcr and sample dilution - (reply: 11)
  77. qPCR result of pooled samples - (reply: 1)
  78. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
  79. qPCR - (reply: 1)
  80. defining ratio of alternative spliced gene with qPCR - (reply: 14)
  81. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
  82. real-time pcr non reproducible - (reply: 4)
  83. Inexplicable qPCR failure in single wells - (reply: 12)
  84. analysing qpcr data and plotting standard curve - (reply: 1)
  85. TaqMan CN assay - control sample duplicated - (reply: 1)
  86. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  87. Basic question about qPCR and endogenous RNA control. - (reply: 4)
  88. CT of qPCR - (reply: 3)
  89. Sybr Green vs Taqman -- a practical approach - (reply: 2)
  90. cDNA amount in qPCR - (reply: 2)
  91. mRNA integrity for qPCR - (reply: 6)
  92. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
  93. Real-time efficiency question - (reply: 1)
  94. Selective removal of standards post qPCR Run - (reply: 1)
  95. qPCR question (no results?) - (reply: 1)
  96. >100% input in histone ChIP qPCR - (reply: 2)
  97. Sensitivity of RT-PCR and qPCR - (reply: 4)
  98. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  99. Are these primer products good enough for qPCR? - (reply: 3)
  100. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
  101. Taqman Probe sequence problem - (reply: 4)
  102. McrBC + qPCR : mock has higher Cts than digested? - (reply: 1)
  103. urgent: need help with qPCR statistics - (reply: 2)
  104. Dye for qPCR - (reply: 3)
  105. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  106. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
  107. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
  108. reference gene for qPCR in E.coli - (reply: 2)
  109. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
  110. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  111. qPCR detection - (reply: 2)
  112. qPCR with Genomic DNA - (reply: 1)
  113. How useful are Service Contracts for qPCR machines? - (reply: 2)
  114. Normalizing qPCR data - (reply: 1)
  115. good 16S univ. primer for qPCR? - (reply: 3)
  116. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
  117. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  118. thermocycler - real-time versus conventional? - (reply: 2)
  119. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
  120. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
  121. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  122. Multiple Bands in qPCR - (reply: 4)
  123. Is it possible to compare abundance of isoforms by qPCR - (reply: 6)
  124. Reference RNA for qPCR - (reply: 3)
  125. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  126. qRT-PCR data analysis - (reply: 2)
  127. Strange RT to qPCR results with new kit - (reply: 5)
  128. qPCR: band in gel, but super high Cq? - (reply: 10)
  129. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  130. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
  131. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  132. qPCR: CT value of reference gene - (reply: 3)
  133. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  134. Making a qPCR standard amplicon from cloning, why? - (reply: 4)
  135. geNorm alternative for qPCR housekeeping genes - (reply: 1)
  136. qPCR contamination problem - (reply: 5)
  137. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  138. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
  139. same primers, different product in conventional PCR and qPCR - (reply: 6)
  140. Plateau height qPCR - (reply: 3)
  141. UDG in qPCR - (reply: 1)
  142. Taqman Probes problem - (reply: 3)
  143. qPCR : why substracting CT values instead of dividing ? - (reply: 3)
  144. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
  145. Problem/qPCR, 2 peaks en melting curve - (reply: 4)
  146. Recommended tamplate DNA concentration for qPCR - (reply: 2)
  147. Enough checks for gDNA contamination in qPCR? - (reply: 3)
  148. optical qPCR plates - (reply: 1)
  149. PCR optimization: PCR vs qPCR - (reply: 4)
  150. TaqMan qPCR low-template issues - (reply: 1)
  151. qPCR standard curve slope (m) - (reply: 2)
  152. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  153. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  154. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  155. Message Amount in qPCR - (reply: 1)
  156. cDNA concentration of qPCR - (reply: 1)
  157. qPCR normalization of reference gene - (reply: 1)
  158. Desperate! qPCR standard curve problems - (reply: 2)
  159. Home made real-time PCR problem - (reply: 4)
  160. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  161. Which Brand of real-time PCR machine - (reply: 6)
  162. Weird qPCR curves - (reply: 11)
  163. Problem with New qPCR primers - help! - (reply: 6)
  164. Validation of microarray data by qPCR - (reply: 1)
  165. Help with high Ct values for Real-Time PCR - (reply: 1)
  166. qRTPCR using taqman - (reply: 4)
  167. Advantage of sybr green over ethidium bromide - (reply: 1)
  168. qPCR analysis- can I use RQ? - (reply: 1)
  169. Error bars for qPCR; technical vs. biological replicate - (reply: 2)
  170. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  171. Too high CT values with TaqMan - (reply: 1)
  172. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
  173. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
  174. Real-Time efficiencies and the pfaffl method - (reply: 1)
  175. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  176. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
  177. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
  178. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  179. qPCR Calc Help - (reply: 6)
  180. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
  181. false positive/cross reaction with TaqMan - (reply: 1)
  182. real time qRT-PCR and low expression gene - (reply: 2)
  183. Validating MeDIP by qPCR - (reply: 3)
  184. Bands seen with qPCR missing in regular PCR - (reply: 5)
  185. How to explain the difference between microarray results and qPCR data - (reply: 1)
  186. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  187. TaqMan Low density Array Card Analysis - (reply: 1)
  188. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  189. Weird qPCR curves - (reply: 4)
  190. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  191. real-time PCR after restriction enzyme digestion - (reply: 6)
  192. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  193. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
  194. Help! Smear in Real-Time PCR Product - (reply: 1)
  195. Sybr green RT-qPCR primer - (reply: 1)
  196. Sybr green RT-qPCR primer - (reply: 1)
  197. running multiple qPCR with common housekeeping control - (reply: 3)
  198. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  199. Consistancy in qPCR data - (reply: 5)
  200. qPCR with low expressing genes - (reply: 1)
  201. Calculating geometric mean for real-time PCR - (reply: 5)
  202. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
  203. >100% qPCR efficiency - (reply: 2)
  204. Improving qRT-PCR detection limits - (reply: 2)
  205. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  206. excel and propagation of error, qpcr - (reply: 1)
  207. Triage of miRNA targets by qPCR - (reply: 1)
  208. qPCR primer design - possible off-target (reply: 1)
  209. Primer dimer formation and real-time PCR - (reply: 7)
  210. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
  211. Sequencing miRNA qRT-PCR Products - (reply: 5)
  212. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
  213. Using DNA as a reference in qRT-PCR - (reply: 1)
  214. minimum replicate for qpcr? - (reply: 15)
  215. qPCR amplification interference fixed with freezing - (reply: 6)
  216. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
  217. qPCR volume - Problem with volume difference and results (reply: 1)
  218. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
  219. Effect of collagenase on qpcr results - (reply: 1)
  220. qPCR inhibition - (reply: 1)
  221. Primers for qPCR - (reply: 3)
  222. can RNA amplify in two-step qPCR? - (reply: 2)
  223. qPCR problem with miRNA - No signal in the qPCR (reply: 4)
  224. Using Sybr green in realtime pcr - (reply: 1)
  225. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
  226. how to determine the amount of cDNA for qPCR - (reply: 5)
  227. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
  228. standard curve for qPCR - (reply: 3)
  229. Standard curve for qPCR - (reply: 1)
  230. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
  231. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
  232. weird QPCR curves - (reply: 2)
  233. Denaturing gel electrophoresis for qPCR - (reply: 3)
  234. Long-amplicon QPCR of mtDNA - (reply: 5)
  235. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
  236. Help with real-time PCR quantification of miRNA - (reply: 8)
  237. negative control in qRT-PCR - (reply: 6)
  238. Question about RealTime PCR Primer Design - (reply: 1)
  239. How to present and analyze these real-time PCR data? - (reply: 2)
  240. qPCR for transformed cells - (reply: 1)
  241. Clinical Real-time PCR assay - (reply: 1)
  242. Standard curve for real-time PCR - (reply: 2)
  243. DNA contamination of RNA samples for qPCR...weird results! - (reply: 3)
  244. Real-time qPCR data analysis - (reply: 1)
  245. Is it possible to compare data between real-time PCR plates - (reply: 1)
  246. Questions of Standards making in relative qRT-PCR - (reply: 2)
  247. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  248. Reference genes and dilutions in qPCR - (reply: 1)
  249. Issue with standard curve for qPCR for CHiP - (reply: 1)
  250. qPCR data analysis - (reply: 1)
  251. qPCR Data analysis - qBasePlus and GenEx (reply: 1)
  252. degenerated Taqman probes? - (reply: 1)
  253. error message on qPCR mashine - (reply: 1)
  254. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  255. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  256. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  257. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
  258. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
  259. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  260. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
  261. qPCR Data Analysis - Software (reply: 3)
  262. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
  263. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  264. Primer design for qPCR - (reply: 2)
  265. Primer design for qPCR - (reply: 1)
  266. reporting results of qPCR - (reply: 1)
  267. question about qRT-PCR preparation - (reply: 1)
  268. qPCR efficiency calculation - (reply: 1)
  269. qPCR on chIP product - (reply: 3)
  270. how to save sybr green reagents? - (reply: 3)
  271. QPCR/DDCT Method/ Statistics - (reply: 2)
  272. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
  273. microRNA profiling using ABI taqman low density microRNA array (TLDA) - (reply: 1)
  274. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  275. Having abnormal dissociation curve when performing qPCR - (reply: 3)
  276. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  277. How much template DNA do you use for qPCR - (reply: 1)
  278. paranormal qpcr amplification activity - melting curves (reply: 3)
  279. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
  280. General qRT-PCR question - (reply: 3)
  281. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
  282. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
  283. qPCR absolute/relative quantification method question - (reply: 4)
  284. Compare different qPCR runs - really need help (reply: 8)
  285. qPCR of BS DNA - (reply: 6)
  286. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  287. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  288. standards for absolute quantification qPCR - (reply: 1)
  289. qPCR, RT- Control - (reply: 2)
  290. Pre-amplification of template for qPCR - (reply: 1)
  291. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
  292. issue with taqman MGB probes - (reply: 2)
  293. conventional v real-time PCR applications - (reply: 1)
  294. different PCR primers for real-time and classic PCR - (reply: 3)
  295. qRT-PCR Inconsistency - (reply: 1)
  296. question qPCR data presentation? - (reply: 1)
  297. increasing qPCR reaction volume - Quick and simple question of qPCR reaction volume (reply: 2)
  298. detect miRNAs expression by qPCR - (reply: 2)
  299. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  300. BSA as qPCR enhancer - NEB or Sigma? (reply: 3)
  301. Reference for qPCR Chip? - (reply: 1)
  302. Workflow of qRT-PCR for siRNA - help please. (reply: 5)
  303. Taqman real-time PCR - Taqman and contamination (reply: 4)
  304. Data Analysis for Real-time PCR - (reply: 2)
  305. Replicate inconsistencies in qPCR - (reply: 6)
  306. Real-Time PCR - Gene expression (reply: 2)
  307. QPCR analysis with various # of samples from 1 patient? - (reply: 1)
  308. multiplexing taqman miRNA reverse transcription - (reply: 2)
  309. Analyzing qPCR results - (reply: 1)
  310. QPCR - Primer and Probe question - QPCR (reply: 2)
  311. qRT-PCR Product Size - (reply: 5)
  312. 18s control in qPCR - (reply: 2)
  313. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  314. qrt-pcr and northern blot ,which way is better? - (reply: 4)
  315. microrna quantitation ---use northern blot or qrt-pcr? - (reply: 1)
  316. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
  317. qPCR analysis - (reply: 6)
  318. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
  319. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  320. calibrator - what is this in qPCR (reply: 1)
  321. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
  322. SYBR Green and Ethidium Bromide in same gel - Just out of curiosity (reply: 2)
  323. Realtime PCR machine - (reply: 2)
  324. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  325. home made sybr green mix for qpcr - (reply: 2)
  326. reference genes for qPCR - (reply: 3)
  327. Fermentas SYBR Green vs. Qiagen SYBR Green - (reply: 2)
  328. qPCR using standard curve method - how to do correct setup? (reply: 3)
  329. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  330. Best time to isolate RNA for qPCR? - (reply: 1)
  331. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  332. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
  333. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
  334. Long real-time Amplicon - (reply: 2)
  335. qPCR - no signal detected but plenty of product on gel - (reply: 3)
  336. Amplify cDNA for qRT-PCR? - (reply: 6)
  337. QPCR problem! - (reply: 9)
  338. Reference gene qPCR - (reply: 4)
  339. qRT-PCR no of cycles?? - (reply: 6)
  340. Is my primer going to work for qRT-PCR? - (reply: 6)
  341. Sybr Green I in real-time PCR reaction - (reply: 3)
  342. Measuring global methylation by QPCR - (reply: 1)
  343. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
  344. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
  345. qRTPCR (TaqMan) Data Representation - (reply: 1)
  346. Ncode qRT-PCR - (reply: 6)
  347. ABI Taqman Low density microRNA Array card - (reply: 3)
  348. RT-PCR - problems with TaqMan PCR (reply: 2)
  349. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
  350. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  351. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  352. Freezing primers+SYBR green - (reply: 2)
  353. differently behavioring replicates in real-time PCR - (reply: 1)
  354. RT qPCR Newbie, Weird Results - (reply: 1)
  355. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  356. qPCR and varying cDNA concentrations - (reply: 1)
  357. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
  358. qPCR and quantity of RNA - (reply: 2)
  359. How & where to prepare qPCR - (reply: 9)
  360. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
  361. Normalization of qpcr data - (reply: 1)
  362. Making standard dilution for qPCR - (reply: 1)
  363. Primers and Taqman Probes mixture question - (reply: 1)
  364. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  365. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  366. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  367. Interpreting QPCR DATA - (reply: 3)
  368. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  369. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
  370. Sequencing of scarce real-time amplicons - (reply: 1)
  371. miRNA real time PCR by SYBR green methods - (reply: 1)
  372. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  373. Real-Time PCR as a Microplate Reader - (reply: 1)
  374. re-amplification in real-time PCR - (reply: 1)
  375. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  376. Ct fluctuations in qPCR - (reply: 3)
  377. qPCR efficiency - how to improve (reply: 4)
  378. qPCR primer design - (reply: 5)
  379. template concentration qPCR - (reply: 1)
  380. qRT-PCR Problems - (reply: 1)
  381. qRT-PCR with DMSO - (reply: 2)
  382. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  383. Problems with qRT-PCR - (reply: 3)
  384. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
  385. Understanding qPCR and gene expression changes - (reply: 10)
  386. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  387. Taqman rtPCR primer and probe design - (reply: 3)
  388. SYBR Green waste removal - (reply: 2)
  389. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  390. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  391. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  392. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  393. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  394. about real time qPCR - (reply: 1)
  395. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  396. SYBR Green miRNA analysis - (reply: 1)
  397. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
  398. Can DEPC-water interfere QPCR reaction? - (reply: 4)
  399. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)
  400. SYBR and TaqMan - (reply: 6)
  401. Need helps with my QPCR result - (reply: 1)
  402. cheap sybr green reagent - (reply: 2)
  403. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  404. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  405. qPCR quantification of ChIP DNA - (reply: 2)