detect miRNAs expression by qPCR - (Mar/24/2010 )
Did anyone here detect miRNAs expression by qPCR? I used the kit from SBI to detect several mature miRNAs expression in several cell lines. I found some of them show single dissociation peak, but some show several peaks. As I have to use mature miRNA sequence as forward primer for qPCR, there is no way to optimize the primer. Can anyone help me with this problem? Thanks a lot.
In general, detecting miRNA by Tagman (ABI) showed higher sensitivity and especially specificity than using SYBR Green (SBI).
Thanks, but tagman is more expensive than sybgreen.