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Help Quesion - (Nov/04/2014 )

I cloned an insert in a vector, but when i transform cell bacteria and do the plasmid insolation i get the plasmid with insert and without insert how to induce or get my plasmid with the insert. 


note the insert has higromycin resistance ¿i can put higromycin and selecte bacteria and avoid replication of the plasmid without insert


You can't - plate for single clones and screen.