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how i can get insert back? - (Aug/14/2010 )

Hi everyone,

can any one tell me how i can get my gene of interest back after having insertion in T/A cloning vector (like pTZ57R/T) as I have no place any restriction sites in gene while doing amplification?

af malik

-af malik-

Do a pcr using the primers that you had cloned your insert, and the cloned vector as your template. is this what you want?

-adrian kohsf-