Must the terminator be in frame? - (Mar/23/2013 )
I cloned a gene into a vector. Stop codon is there. The terminator comes immediately thereafter, but unfortunately not in frame.
What will happen?
The manuals for some commercial vectors, such as pET, say that the terminator isn't quite necessary. My vector is self-made and the promoter and terminator are specific.
What kind of terminator?
There is no logical reason for it to be in frame afaik, since it's role is to to detach polymerase, RNApol doesn't care whether someting is in frame or not, ribosomes do care.
Furthermore, after stop codon, no "frame" longer exists actually.