GFP -weak signal -why? - (Apr/02/2013 )
I have cloned various inserts into a GFP vector. I can still see the GFP after the successful clonings however the signal is much weaker compared to the original GFP vector. I need full strength of the GFP. What do I do?
Insert a spacer region between the GFP and my DNA sequence? Is it possible that my insert somehow inhibits the GFP signal?
Could be that the folding of the finished protein alters the GFP structure and hence fluorescence. It could also be that the constructs don't transfect as well as the "empty" vector.