Use magnetic beads to purify restriction enzyme digestion product - (Jul/07/2013 )
I am asking whether it is possible to use magnetic beads (Ampure beads from beckman) to purify restriction enzyme digestion product instead of
CIAP is a horrible enzyme. My first step would be to design a protocol to eliminate its use. Switch to a temperature sensitive phosphatase, or eliminate the phosphatase entirely. Then, you can heat inactivate and then use beads to purify.