Pseudomonas aeruginosa 14 with pUC19? - (Jul/08/2013 )
I was wondering if anyone can tell me if a plasmid (pDsRed-Express2**) which has a pUC19 "backbone" be expressed in Pseudomonas aeruginosa 14?
The recommended propagation strain is E. coli DH5a but I wanted to electroporate the plasmid into PA14.
Can someone tell me if this is possible or if there are any articles that have done this?
It will probably work.
I copied the info below from the manual of pDsRed-Express2 vector, hope it's helpful for you.
pDsRed-Express2 is primarily intended to serve as a source of DsRed-Express2 cDNA. The flanking MCS
regions make it possible to excise the DsRed-Express2 coding sequence and insert it into other vector
systems. The vector can also be used to express DsRed-Express2 in bacteria.