antibiotics Methods, Protocols and Troubleshootings

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Antibiotics Related Discussions

can i use antibiotics with lipofectamine - my cells have antibiotic as selective marker and need neomycin (reply: 4)
development of antibiotic resistance - after resistance development, how can i upgrade my work? pls help (reply: 2)
contransfection why choose beta galactocidase? - why not an antibiotic resistant plasmid (reply: 5)
why use beta galactocidase as a positive control in co-transfection - why not an antibiotic resistant plasmid (reply: 13)
making lb + amp broth - question (reply: 3)
cotransfection selection plasmid - need antibiotic marker rather than B galactocidase (reply: 1)
can you freeze media with antibiotics in it - (reply: 1)
antibiotic stability in plates at room temperature? - (reply: 2)
Bisulfite sequencing reproducibility - overlapping PCR amp primers give varying results (reply: 2)
Kanamycin resistance gene in Gram-positive bacteria - (reply: 2)
confused with plots - Rn vs. cycle & delta Rn vs. cycle - (reply: 1)
Protocol for H&E staining for histopathology - (reply: 2)
which company have the best G418 ？ - (reply: 2)
chromatids in mitosis & meiosis - quick que (reply: 2)
lost of antibiotic resistance - do you know? (reply: 1)
Cambell & Reece 7th ed test bank - Test Bank (reply: 1)
PCR bands - why not straight ?&%#@&& - (reply: 3)
difference between linkage & linkage disequilibrium - population genetics beginner! (reply: 1)
vector seq & Multiple cloning site design. - (reply: 2)
I AM KANAMYCIN RESISTANT! - (reply: 10)
2 questions: yeast & C18 - (reply: 1)
PMA / Ionomycin, Revised & Simplified Question, Please Help! - (reply: 1)
TC Hood & incubator contamianted - (reply: 3)
books for microarray data analysis & visualization - (reply: 3)
A few questions on SDS, coomassie staining & western - (reply: 6)
Kanamycin selection of transgenic Arabidopsis - (reply: 3)
Transfection & Wound Healing Assay - (reply: 2)
Native & SDS - TOTALLY CONFUSED,kindly help me (reply: 7)
How kanamycin resistant work? Why colonies and not lawn in plate with ampicillin - (reply: 1)
fixation & decalcification - (reply: 2)
Current Protocol by John Wiley & Son - limited free access from invitrogen (reply: 1)
excessive ampicillin in L.B/Amp plates - (reply: 4)
asking for kanamycin stock solution... - (reply: 4)
carbenicillin/ampicillin - (reply: 3)
Any experience with "Strep tag" fusion peptides & Strep-Tactin pur - (reply: 1)
MCF-7 & MTT assay & E-screen - (reply: 1)
Fishman units of beta-glucuronidase & Roy units of arylsulfatase - (reply: 1)
How to do mutations & rescue experiments - (reply: 1)
Knock -in & mutation experiments - Knock -in & mutation experiments (reply: 2)
Rescue experiments & introducing mutations - Rescue experiments & introducing mutations (reply: 1)
Failed RT-PCR amplification of 3-&5''-ligated small RNAs - (reply: 5)
antibiotics database - no such database ? (reply: 3)
ammonium sulfate precipitation & urea - (reply: 9)
recommended primers for caspase 8 & 9 - human (reply: 4)
Could the strange G418 resistance fo my Chinese hamser cell lines be due to the - CAN I USE UNTREAED plates for selection? (reply: 2)
Rescuing contaminated S. cerevisiae culture with antibiotics? - (reply: 1)
High concentration of antibiotics in amplification of plasmids - concerns about mutation of inserts in plasmid (reply: 1)
Does kanR gene confer resistance to Ampicillin? - (reply: 1)
aminoglycoside antibiotic.. rp-isocratic lc.. no retention? - (reply: 2)
cell lysate PAGE & blotting problem? - (reply: 1)
easy & quick apoptosis assay for siRNA treated cell line sample - (reply: 3)
ChIP Fixation & Protease inhibitors - (reply: 1)
Post sonication RNAse & Prot K treatment - What are the bands? (reply: 15)
gel percentage & loading control for ATM and ATR? - western blotting (reply: 6)
help & question - (reply: 4)
stable transfected cell line in absence of G418 - (reply: 6)
Non toxic H&E staining? - (reply: 1)
colonies grow in amp plates not in broth - (reply: 6)
kanamycin estimation assay? range 0-50 microgram per ml - does anyone have any methods for it? thanks! (reply: 8)
Buffer Exchange & Protein Purification - (reply: 1)
storage of antibiotics - (reply: 3)
what to dissolve antibiotic in - (reply: 1)
Antibiotic resistance-GFP fusions working? - Anybody with experience with pTracer and friends? (reply: 3)
SDS-page & lipidated proteins - (reply: 6)
2 easy & 1 difficult questions - (reply: 4)
cell harvesting; use of antibiotics? - (reply: 8)
Problems with G418 selection - (reply: 1)
Soft Agar colony formation assay and G418 - (reply: 2)
Why cells are so resistant to G418? - G418 cannot kill cells (reply: 6)
miRNA & mRNA microarray data correlation - is there any software that can correlate miRNA and mRNA data? (reply: 4)
Thiamine & pyridoxine HCL in MS medium - (reply: 1)
Reuse of LB Amp plates? - (reply: 7)
No expression from injected GFP&RFP mRNA - (reply: 16)
ANTIBIOTIC RESISTANCE - (reply: 1)
validating the efficiency of inhibitors of protein kinases A & G - (reply: 3)
Ampicillin stability over 14 years - (reply: 5)
GFP/RFP/YFP G418 resistant plasmids - Help please (reply: 1)
Trypan blue & methylene blue - both to measure cell viability? (reply: 2)
neomycin-resistant gene vs kanamycin - (reply: 3)
Zebrafish embryos time & temperature - (reply: 4)
stability of antibiotics. - (reply: 1)
stability of ampicillin n kanamycin plates. - (reply: 4)
detailed function of na-acetate & chloroform:isoamylalcohol in RNA extractio - (reply: 1)
Antibiotics in Cryoprotectant medium? - (reply: 3)
please help in Anthurium & Orchid Tissue culture - tissue culture medium (reply: 2)
can blunt & sticky ends stick - (reply: 11)
G418 Kill Curve - Question (reply: 2)
G418 and hygromycin selection question - (reply: 3)
Can bacteria grow without a plasmid or am i stupid? - or did ampicillin wear out? (reply: 5)
Urgent amp agar plate question! - Help with stupid question (reply: 22)
Why is an antibiotic free media necessary before transfection? - (reply: 4)
serum free culture - doubts about adaptation n antibiotics (reply: 6)
E.coli are growing in LB/Amp plates but not in liquid medium - (reply: 6)
co-transfection & transfection - (reply: 3)
help with reading H&E slides - link to H&E guide? (reply: 1)
What to dissolve hygromycin and G418 with? - (reply: 3)
short term & economical preservation of culture - (reply: 1)
intornic & intergenic EST? - (reply: 3)
coupling surface biotinylation & co-immunoprecipitation? - (reply: 3)
G418 and resistant cells - can't kill the cells (reply: 8)
Ampicillin powder stability - (reply: 3)
Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
cell signaling & choice of the cell - (reply: 3)
Confluence & Differentiation in PC-12 Cultures - How is Confluence in PC-12's Determined? Differentiation? (reply: 2)
New to cloning...Help needed..DH5 alpha cells - Does Dh5alpha have antibiotic resistance to Amp? (reply: 3)
Differences between DGGE & standard electrophoresis? - (reply: 1)
Immunoprecipitation & Mass Spec analysis - How much mg(ug) of protein lysate is needed for IP followed by Mass Sp (reply: 2)
mutant protein expression & purification problem in E.coli - Protein with five residues mutated to Alanine's (reply: 6)
Contamination of antibiotic solutions - (reply: 5)
PCR protocols - for some antibiotic resistant strains (reply: 2)
G418 selection protocol - need ideas for G418 selection (reply: 5)
G418 effect on protein concentration - (reply: 1)
Plasmid stability over a 100%? - Why does ampicillin increase cell viability? (reply: 4)
false positive clones URGENT - resitant to G418 but not expressing protein (reply: 13)
Cells grow on Amp plates but not in LB with Amp - Amp problem, bug strain problem, or ligation problem ? or all of the a (reply: 7)
G418 Killing curve - How to determinate the right G418 concentration by killing curve ? (reply: 5)
Cloning & Expression - Cloning & Expression (reply: 7)
Cells are not dying when I do the killing curve with G418 - (reply: 5)
H&E lithium carbonate - staining (reply: 2)
H&E staining - method (reply: 4)
hek293t and geneticin G418 - (reply: 4)
faint band of interest & primer-dimer prob - (reply: 5)
difference between NIH3T3 & 3T3-L1 - (reply: 9)
G418 resistant 293 - (reply: 4)
Fix&Perm, biotin-SA protocols, blocking of nonspec. binding - (reply: 4)
BigDye® v1.1 & v3.1 5X Sequencing Buffer Makeup? - (reply: 1)
Collection of selective antibiotic conc for ALL cell lines - Please add yours! (reply: 6)
What is the right G418 concentration for selection on TSA and HEK? - (reply: 8)
after 6 weeks of selection in G418? - (reply: 4)
Can I use AMP plates with Agrobacterium? - (reply: 1)
Cell culture contamination, antibiotic recommendation, any recommd on Normocin - (reply: 7)
Stable transfection utilizing ampicillin resistance? - (reply: 2)
Kanamycin - quality of kanamycin (reply: 5)
Which antibiotic to choose? - puro vs hygro vs zeo etc (reply: 4)
storage of media with antibiotics at 4C.... - (reply: 7)
Kit for cyclic AMP assay - (reply: 2)
Gel Electrophoresis & Staining - (reply: 2)
A260/280 & A260/230 Parametrs for determining purity of a nucleic acid sampl - (reply: 4)
Rat & Mouse Brain - (reply: 3)
Kanamycin in a F' strain? - problems with tn5? (reply: 2)
Does LB AMP XGal IPTG plates - for only Amp selection.. (reply: 4)
Durability of pcr-machines - 4°C & peltier-elements (reply: 6)
Dilution Techniques & Calculations - (reply: 3)
Kanamycin/Neomycin resistance - (reply: 3)
How a copy no.& insert no. could be find out in a transgenics - (reply: 1)
antibiotic detection from mold - (reply: 1)
Promoter Sequences - E.coli sequence & determining the promoter (reply: 4)
forward & reverse primers - (reply: 3)
SDS-PAGE precipitation& denaturation - (reply: 3)
G418 conc for HEK293 cells - (reply: 11)
Primers & probes of Applied Biosystems for real time - (reply: 2)
collagen H&E staining - tissue collagen staining (reply: 3)
adding antibiotics to already made YT plates - (reply: 6)
difference between RNA & cDNA gel image - (reply: 1)
antibiotic selection for breast cancer MDA-MB-436 - (reply: 1)
question about glacial acetic acid - glacial acetic acid & acetic acid (reply: 2)
MDA-MB-231 antibiotic - (reply: 2)
Precipitate in PCR buffer w/BSA & 2-mercaptoethanol - PCR buffers (reply: 1)
nutrition selection or antibiotic selection - (reply: 2)
Competant cells & incompetant scientist - No colonies formed (reply: 8)
selection of stable transfectants with ampicillin - (reply: 4)
LC sample load & solubility advice? - Shimadzu prominence (reply: 4)
Glycoprotein sequencing & PVDF membrane transfer - (reply: 3)
amp v kan/neo resistance query - (reply: 3)
LB agar with fungizone & nystatin - (reply: 4)
Mutagenesis Help (plasmid isolation & sequencing stages) - (reply: 2)
can any one suggest list of protease inhibitors list & if possible reference - (reply: 2)
kanamycin activity with time - (reply: 4)
Antibiotic after transfection or not? - (reply: 2)
Afl II linearization & ligation - (reply: 3)
PC3 Cells & DU145 Cells - Cell Line (reply: 2)
Homo & heterodimer Problem - (reply: 5)
Bands appear poor/not at all and do not appear consistantly & primer bands a - (reply: 2)
PCR amp. - sequencing problem - (reply: 3)
Geneticin (G418) Killing Curve - Which is the appropriate incubation time to assess viabiliy? (reply: 1)
Annexin staining - strange & mysterious population - (reply: 3)
thermophilic cellulolytic fungi isolation & charactrisation of of such enzym - thermophilic cellulolytic fungi isolation & charactrisation of of such enzym (reply: 1)
problem with culture revival & passage - (reply: 1)
Use of antibiotics in cell culture? - (reply: 5)
Problem with cell line/G418 - (reply: 8)
RNA extraction after H&E - (reply: 1)
G418 selection - (reply: 1)
What is the difference between double distiled water & RO water? - (reply: 3)
Slow growing cells (MCF-7 & MDA-MB231) - (reply: 7)
induction estrogen responsive gene (PS2 & PgR) in MCF7 - (reply: 3)
Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
Raw 265.7 & FBS - (reply: 1)
Gel percentage & cross-linker percentage, protein MW constrains. - (reply: 2)
adding antibiotics to cho cell media - (reply: 2)
Filtering & removing non-sequence data from a FASTA FILE using Perl scripts. - (reply: 9)
Why 70% ethanol & not 95% as disinfectant? - (reply: 25)
Antibiotics and infection - (reply: 3)
which antibiotic for primary culture? - (reply: 3)
about G418 and neomycin - is there any difference? the solution? (reply: 10)
Antibiotic concentration for selection of transfectants - (reply: 7)
stable transfectant - is there a way to make stables without antibiotic resistance? (reply: 2)
G418 selection problem - (reply: 4)
Plate storage & picking colonies - How much time do I have? (reply: 4)
why selection results are so different with different antibiotics? - (reply: 3)
Cells survived after G418 selection are transfected cells? - (reply: 2)
Trouble growing bacteria in LB/amp solution - (reply: 5)
Gel permeation & filtration - difference plz? (reply: 1)
DNA (Major groove & minor Groove) - BAse pairs (reply: 1)
structure difference between plasmid & chromosomal DNA - (reply: 1)
Specific Specificity! - abt L/D- AAs ,D/L-sugars & all... (reply: 1)
PCR my cDNA & got nothing - (reply: 2)
Antibiotic investigation - (reply: 1)
cDNA precipitate & DNA ladders - (reply: 4)
HELP: HEK293T cells and geneticin (G418) - (reply: 1)
Did anyone here use neomycin to kill MCF-7 cells? - (reply: 4)
What is the transport mechanism of lipophilic drugs/agents into a cell? - Cell Membrane & Apoptosis (reply: 3)
about antibiotic in plasmid transformation - which is best? (reply: 6)
double digest pcDNA(+) by XhoI&XbaI, uncompletely? - (reply: 3)
who can tell me the valid period of ampicillin plate? - (reply: 3)
question about antibodies & protein G/A sepharose in CO-IP - (reply: 6)
Stable transfectants of HEK293 (selection with G418). Question about splitting ( - Cells growing confluent long before G418 kills them, is splitting smart or not? (reply: 9)
How U can read map of DNA & some questions - recognize Map (reply: 3)
Looking for a kanamycin cassette - (reply: 2)
LB with or without antibiotic - (reply: 3)
superoxide production & PMA - (reply: 1)
LB + Amp. preparation - Recipes (reply: 6)
sds page of protein in tfa & acotonitrile - (reply: 1)
LB + X-gal plates + Amp plates - (reply: 8)
Unexpected antibiotics resistance - (reply: 4)
ampicillin resistance in Staphylococcus aureus - (reply: 1)
Troubleshooting a Low 260/280 DNA ratio & Phenol storage - (reply: 2)
FBS & FCS: any difference? - (reply: 2)
Making ampicillin plates from plain LB plates - Making amp plates from prepoured LB plates (reply: 2)
Difference between Random & Arbitrary Primers - What is it? (reply: 1)
antibiotics light sensitivity - How sensitive are antibiotics really? (reply: 1)
How long can LB plates with antibiotics be stored at 4C - (reply: 5)
Sonication & RNA - (reply: 1)
DTT & RNase inhibitor in RT reaction - (reply: 6)
Problems cloning into pET Kanamycin resistance vectors - (reply: 7)
Antibiotic resistant mutant - Antibiotic resistance in plant groth promoting bacteria. (reply: 1)
Antibiotic marker expression in e.coli and geobacilli - (reply: 1)
Antibiotic Resistance - MIC interpretive standards for streptomycin susceptibility (reply: 3)
pcr - etbr & dna extract (reply: 2)
Ethics & Pandora's box - (reply: 4)
Slit cells or add antibiotics, which is first after stable transfection - (reply: 8)
What are EST,accession number,symbol,GeneID&RefSeq? - anyone can give me answer please.... (reply: 3)
G418 concentration - G418 concentration (reply: 5)
pcDNA3.1 & pBIG2i vectors appear "bigger" on the gel! :( - (reply: 1)
Buying Pure Antibacterials/Antibiotics - Where Can I Buy Absolute Antibacterials/Antibiotics? (reply: 6)
antibiotics for 3 vectors and sequencing - (reply: 2)
G418 vs Neomycin? - (reply: 8)
antibiotic dose response for suspension cell - (reply: 2)
nutrients & gene expression - (reply: 2)
change between neomycin and blasticidin resistance gene - (reply: 10)
Direction of Amp cassette - (reply: 6)
Anyone use puromycin and blasticidin as selective Antibiotic for HeLa - (reply: 2)
Which MSP & BGS primer design tool do you "swear" by? - (reply: 9)
PCR of AmpR gene - PCR of Amp gene (reply: 1)
Methylation & PCR - (reply: 1)
What happens if I leave an antibiotic under UV overnight? - HELP!! (reply: 1)
DNA & RNA Isolation - can any person give me protocol for this (reply: 4)
Growing conditions for OE21 & OE33 cells - (reply: 1)
antibiotic from actinomycetes - (reply: 2)
Difference between phosphate buffer & potassium phosphate buffer - (reply: 2)
Viral RNA extraction - Formalin fixed & Paraffin embended tissue (reply: 17)
LB Amp Agar Shelf life - LB Plate Shelf Life (reply: 2)
G418 Stable Transfection - (reply: 4)
antibiotics cell transfection - transfection (reply: 2)
Pour plate & spread plate - Merits of pour plates (reply: 7)
pGen.IRES.GFP.Neo - (reply: 3)
G418 in freezing medium? - (reply: 2)
Will the amount of G418 be affected by serum concentration? - (reply: 3)
How much an H-bond can contribute to protein intergrity? - Protein structure & hydrogen bonds (reply: 2)
Internal Control RNA & RT-PCR - (reply: 1)
RNA isolation - combination of Trizol & colum-based kit (reply: 6)
A problem about G418 selection - (reply: 3)
MCF7 & MDA MB231 transfection - (reply: 3)
antibiotics concentration for JM109 and DH5 alpha - (reply: 3)
antibiotics concentration for ligated plasmid - (reply: 3)
Basic question - making antibiotic plates - (reply: 5)
plasmid extraction and antibiotics matters... - (reply: 4)
What causes low 260/230 ratio for RNA & how to correct it - (reply: 14)
antibiotics of bacillus subtilis - (reply: 2)
Clean & Concentrate DNA without Kit - (reply: 1)
Stable cell line - G418 - (reply: 5)
Can G418 be used to kill bacteria? - (reply: 6)
antibiotics in medium-WHEN? - (reply: 10)
Antibiotics selection in mammalian expression - (reply: 3)
Best time for adding G418? - transfection and selecting (reply: 2)
Antibiotic use in Pichia cultures - (reply: 1)
Viral RNA & DNA extraction from serum - Protocols (reply: 1)
Sex pilus & flagellum - (reply: 2)
AB resistance gene - Which antibiotic resistance gene in my vector ? (reply: 3)
Removal of endotoxin from proteins & DNA - Protein & DNA purification (reply: 15)
difference between LB Miller & LB, Lennox - difference between LB Miller & LB, Lennox (reply: 2)
Antibiotics during siRNA transfection - (reply: 4)
old degraded samples. DNA extraction & PCR - (reply: 3)
hep-2 & Hep-G2 cell line - difference between hep-2 & Hep-G2 cell line (reply: 22)
How long time needed to determine the concentration of G418? - (reply: 2)
agar for antibiotic sensitivity testing - (reply: 1)
Haematoxylin Eosin staining for frozen sections - H&E staining frozen sections (reply: 4)
Problem Testing Antibiotic Sensitivity of Clumping Bacteria - And to do so without vigorous shaking.... (reply: 4)
digestion & purification problem - (reply: 6)
help:detailed steps of doing blast search on PC - Blast program & perl innterface (reply: 3)
PCR Cloning - Please Help - Urgent - Cloning using SalI & BamHI site (reply: 1)
cell count & viability - does DMSO affect trypanblue staining after thawing (reply: 6)
PCR-Check, Screen-Check, MiniPrep - HUH?? - PCR product transformed, grown on AMP, no mini (reply: 2)
who can tell me molecular basis of "dominant" & "re - "dominant" & "recessive" (reply: 1)
Selection of clones during transfection - Concentration of G418 to be added to the cell. (reply: 7)
footprinting & gelshift - (reply: 1)
How to create my own database and do local blast? - Local database & BLAST (reply: 2)
Cloning Problems - #@%$&*^) (reply: 5)
Wester VS Immunoprecipitation -Da Paradigm...?!?! - Western Blot & Immunoprecipitaion & Ab (reply: 3)
TCR Zeta Chain & Phospho-species - immunoprecipitation and WB (reply: 1)
rRNA in insects - Information about 18S, 5.8S, ITS1 & ITS2 (reply: 1)
Q&A - You got questions, we get answers (reply: 1)
Formalin fixation&paraffin-embedding of tissue culture cells - Formalin fixation&paraffin-embedding of tissue culture cells (reply: 1)
Detect PPAR gamma in fat&muscle tissue - protein extract (reply: 1)
Antibiotic working concentratios. - (reply: 2)
Murine MAb IgG purification - monoclonal antibody purification from ascites & supernatant (reply: 1)
G418 storage and contamination - (reply: 2)
DNA precipitation by EtOH &centrifuge - (reply: 4)
RNA & DNA extractionprotocols - (reply: 2)