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Antibiotics Related Discussions
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development of antibiotic resistance - after resistance development, how can i upgrade my work? pls help (reply: 2)
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why use beta galactocidase as a positive control in co-transfection - why not an antibiotic resistant plasmid (reply: 13)
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cotransfection selection plasmid - need antibiotic marker rather than B galactocidase (reply: 1)
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antibiotic stability in plates at room temperature? - (reply: 2)
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Kanamycin resistance gene in Gram-positive bacteria - (reply: 2)
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Protocol for H&E staining for histopathology - (reply: 2)
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chromatids in mitosis & meiosis - quick que (reply: 2)
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Cambell & Reece 7th ed test bank - Test Bank (reply: 1)
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difference between linkage & linkage disequilibrium - population genetics beginner! (reply: 1)
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I AM KANAMYCIN RESISTANT! - (reply: 10)
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PMA / Ionomycin, Revised & Simplified Question, Please Help! - (reply: 1)
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books for microarray data analysis & visualization - (reply: 3)
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Kanamycin selection of transgenic Arabidopsis - (reply: 3)
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Native & SDS - TOTALLY CONFUSED,kindly help me (reply: 7)
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fixation & decalcification - (reply: 2)
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excessive ampicillin in L.B/Amp plates - (reply: 4)
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carbenicillin/ampicillin - (reply: 3)
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MCF-7 & MTT assay & E-screen - (reply: 1)
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How to do mutations & rescue experiments - (reply: 1)
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Rescue experiments & introducing mutations - Rescue experiments & introducing mutations (reply: 1)
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antibiotics database - no such database ? (reply: 3)
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recommended primers for caspase 8 & 9 - human (reply: 4)
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Rescuing contaminated S. cerevisiae culture with antibiotics? - (reply: 1)
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Does kanR gene confer resistance to Ampicillin? - (reply: 1)
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cell lysate PAGE & blotting problem? - (reply: 1)
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ChIP Fixation & Protease inhibitors - (reply: 1)
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gel percentage & loading control for ATM and ATR? - western blotting (reply: 6)
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stable transfected cell line in absence of G418 - (reply: 6)
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colonies grow in amp plates not in broth - (reply: 6)
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Buffer Exchange & Protein Purification - (reply: 1)
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what to dissolve antibiotic in - (reply: 1)
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SDS-page & lipidated proteins - (reply: 6)
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cell harvesting; use of antibiotics? - (reply: 8)
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Soft Agar colony formation assay and G418 - (reply: 2)
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miRNA & mRNA microarray data correlation - is there any software that can correlate miRNA and mRNA data? (reply: 4)
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Reuse of LB Amp plates? - (reply: 7)
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ANTIBIOTIC RESISTANCE - (reply: 1)
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Ampicillin stability over 14 years - (reply: 5)
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Trypan blue & methylene blue - both to measure cell viability? (reply: 2)
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Zebrafish embryos time & temperature - (reply: 4)
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stability of ampicillin n kanamycin plates. - (reply: 4)
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Antibiotics in Cryoprotectant medium? - (reply: 3)
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can blunt & sticky ends stick - (reply: 11)
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G418 and hygromycin selection question - (reply: 3)
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Urgent amp agar plate question! - Help with stupid question (reply: 22)
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serum free culture - doubts about adaptation n antibiotics (reply: 6)
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co-transfection & transfection - (reply: 3)
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What to dissolve hygromycin and G418 with? - (reply: 3)
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intornic & intergenic EST? - (reply: 3)
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G418 and resistant cells - can't kill the cells (reply: 8)
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Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
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Confluence & Differentiation in PC-12 Cultures - How is Confluence in PC-12's Determined? Differentiation? (reply: 2)
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Differences between DGGE & standard electrophoresis? - (reply: 1)
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mutant protein expression & purification problem in E.coli - Protein with five residues mutated to Alanine's (reply: 6)
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PCR protocols - for some antibiotic resistant strains (reply: 2)
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G418 effect on protein concentration - (reply: 1)
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false positive clones URGENT - resitant to G418 but not expressing protein (reply: 13)
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G418 Killing curve - How to determinate the right G418 concentration by killing curve ? (reply: 5)
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Cells are not dying when I do the killing curve with G418 - (reply: 5)
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H&E staining - method (reply: 4)
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faint band of interest & primer-dimer prob - (reply: 5)
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G418 resistant 293 - (reply: 4)
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BigDye® v1.1 & v3.1 5X Sequencing Buffer Makeup? - (reply: 1)
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What is the right G418 concentration for selection on TSA and HEK? - (reply: 8)
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Can I use AMP plates with Agrobacterium? - (reply: 1)
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Stable transfection utilizing ampicillin resistance? - (reply: 2)
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Which antibiotic to choose? - puro vs hygro vs zeo etc (reply: 4)
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Kit for cyclic AMP assay - (reply: 2)
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A260/280 & A260/230 Parametrs for determining purity of a nucleic acid sampl - (reply: 4)
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Kanamycin in a F' strain? - problems with tn5? (reply: 2)
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Durability of pcr-machines - 4°C & peltier-elements (reply: 6)
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Kanamycin/Neomycin resistance - (reply: 3)
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antibiotic detection from mold - (reply: 1)
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forward & reverse primers - (reply: 3)
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G418 conc for HEK293 cells - (reply: 11)
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collagen H&E staining - tissue collagen staining (reply: 3)
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difference between RNA & cDNA gel image - (reply: 1)
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question about glacial acetic acid - glacial acetic acid & acetic acid (reply: 2)
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Precipitate in PCR buffer w/BSA & 2-mercaptoethanol - PCR buffers (reply: 1)
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Competant cells & incompetant scientist - No colonies formed (reply: 8)
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LC sample load & solubility advice? - Shimadzu prominence (reply: 4)
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amp v kan/neo resistance query - (reply: 3)
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Mutagenesis Help (plasmid isolation & sequencing stages) - (reply: 2)
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kanamycin activity with time - (reply: 4)
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Afl II linearization & ligation - (reply: 3)
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Homo & heterodimer Problem - (reply: 5)
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PCR amp. - sequencing problem - (reply: 3)
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Annexin staining - strange & mysterious population - (reply: 3)
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problem with culture revival & passage - (reply: 1)
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Problem with cell line/G418 - (reply: 8)
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G418 selection - (reply: 1)
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Slow growing cells (MCF-7 & MDA-MB231) - (reply: 7)
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Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
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Gel percentage & cross-linker percentage, protein MW constrains. - (reply: 2)
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Filtering & removing non-sequence data from a FASTA FILE using Perl scripts. - (reply: 9)
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Antibiotics and infection - (reply: 3)
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about G418 and neomycin - is there any difference? the solution? (reply: 10)
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stable transfectant - is there a way to make stables without antibiotic resistance? (reply: 2)
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Plate storage & picking colonies - How much time do I have? (reply: 4)
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Cells survived after G418 selection are transfected cells? - (reply: 2)
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Gel permeation & filtration - difference plz? (reply: 1)
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structure difference between plasmid & chromosomal DNA - (reply: 1)
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PCR my cDNA & got nothing - (reply: 2)
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cDNA precipitate & DNA ladders - (reply: 4)
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Did anyone here use neomycin to kill MCF-7 cells? - (reply: 4)
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about antibiotic in plasmid transformation - which is best? (reply: 6)
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who can tell me the valid period of ampicillin plate? - (reply: 3)
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Stable transfectants of HEK293 (selection with G418). Question about splitting ( - Cells growing confluent long before G418 kills them, is splitting smart or not? (reply: 9)
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Looking for a kanamycin cassette - (reply: 2)
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superoxide production & PMA - (reply: 1)
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sds page of protein in tfa & acotonitrile - (reply: 1)
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Unexpected antibiotics resistance - (reply: 4)
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Troubleshooting a Low 260/280 DNA ratio & Phenol storage - (reply: 2)
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Making ampicillin plates from plain LB plates - Making amp plates from prepoured LB plates (reply: 2)
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antibiotics light sensitivity - How sensitive are antibiotics really? (reply: 1)
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Sonication & RNA - (reply: 1)
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Problems cloning into pET Kanamycin resistance vectors - (reply: 7)
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Antibiotic marker expression in e.coli and geobacilli - (reply: 1)
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pcr - etbr & dna extract (reply: 2)
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Slit cells or add antibiotics, which is first after stable transfection - (reply: 8)
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G418 concentration - G418 concentration (reply: 5)
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Buying Pure Antibacterials/Antibiotics - Where Can I Buy Absolute Antibacterials/Antibiotics? (reply: 6)
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G418 vs Neomycin? - (reply: 8)
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nutrients & gene expression - (reply: 2)
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Direction of Amp cassette - (reply: 6)
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Which MSP & BGS primer design tool do you "swear" by? - (reply: 9)
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Methylation & PCR - (reply: 1)
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DNA & RNA Isolation - can any person give me protocol for this (reply: 4)
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antibiotic from actinomycetes - (reply: 2)
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Viral RNA extraction - Formalin fixed & Paraffin embended tissue (reply: 17)
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G418 Stable Transfection - (reply: 4)
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Pour plate & spread plate - Merits of pour plates (reply: 7)
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G418 in freezing medium? - (reply: 2)
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How much an H-bond can contribute to protein intergrity? - Protein structure & hydrogen bonds (reply: 2)
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RNA isolation - combination of Trizol & colum-based kit (reply: 6)
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MCF7 & MDA MB231 transfection - (reply: 3)
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antibiotics concentration for ligated plasmid - (reply: 3)
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plasmid extraction and antibiotics matters... - (reply: 4)
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antibiotics of bacillus subtilis - (reply: 2)
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Stable cell line - G418 - (reply: 5)
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antibiotics in medium-WHEN? - (reply: 10)
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Best time for adding G418? - transfection and selecting (reply: 2)
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Viral RNA & DNA extraction from serum - Protocols (reply: 1)
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AB resistance gene - Which antibiotic resistance gene in my vector ? (reply: 3)
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difference between LB Miller & LB, Lennox - difference between LB Miller & LB, Lennox (reply: 2)
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old degraded samples. DNA extraction & PCR - (reply: 3)
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How long time needed to determine the concentration of G418? - (reply: 2)
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Haematoxylin Eosin staining for frozen sections - H&E staining frozen sections (reply: 4)
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digestion & purification problem - (reply: 6)
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PCR Cloning - Please Help - Urgent - Cloning using SalI & BamHI site (reply: 1)
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PCR-Check, Screen-Check, MiniPrep - HUH?? - PCR product transformed, grown on AMP, no mini (reply: 2)
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Selection of clones during transfection - Concentration of G418 to be added to the cell. (reply: 7)
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How to create my own database and do local blast? - Local database & BLAST (reply: 2)
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Wester VS Immunoprecipitation -Da Paradigm...?!?! - Western Blot & Immunoprecipitaion & Ab (reply: 3)
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rRNA in insects - Information about 18S, 5.8S, ITS1 & ITS2 (reply: 1)
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Formalin fixation¶ffin-embedding of tissue culture cells - Formalin fixation¶ffin-embedding of tissue culture cells (reply: 1)
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Antibiotic working concentratios. - (reply: 2)
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G418 storage and contamination - (reply: 2)
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RNA & DNA extractionprotocols - (reply: 2)