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Antibiotics Related Discussions
  1. development of antibiotic resistance - after resistance development, how can i upgrade my work? pls help (reply: 2)
  2. why use beta galactocidase as a positive control in co-transfection - why not an antibiotic resistant plasmid (reply: 13)
  3. cotransfection selection plasmid - need antibiotic marker rather than B galactocidase (reply: 1)
  4. antibiotic stability in plates at room temperature? - (reply: 2)
  5. Kanamycin resistance gene in Gram-positive bacteria - (reply: 2)
  6. Protocol for H&E staining for histopathology - (reply: 2)
  7. chromatids in mitosis & meiosis - quick que (reply: 2)
  8. Cambell & Reece 7th ed test bank - Test Bank (reply: 1)
  9. difference between linkage & linkage disequilibrium - population genetics beginner! (reply: 1)
  10. I AM KANAMYCIN RESISTANT! - (reply: 10)
  11. PMA / Ionomycin, Revised & Simplified Question, Please Help! - (reply: 1)
  12. books for microarray data analysis & visualization - (reply: 3)
  13. Kanamycin selection of transgenic Arabidopsis - (reply: 3)
  14. Native & SDS - TOTALLY CONFUSED,kindly help me (reply: 7)
  15. fixation & decalcification - (reply: 2)
  16. excessive ampicillin in L.B/Amp plates - (reply: 4)
  17. carbenicillin/ampicillin - (reply: 3)
  18. MCF-7 & MTT assay & E-screen - (reply: 1)
  19. How to do mutations & rescue experiments - (reply: 1)
  20. Rescue experiments & introducing mutations - Rescue experiments & introducing mutations (reply: 1)
  21. antibiotics database - no such database ? (reply: 3)
  22. recommended primers for caspase 8 & 9 - human (reply: 4)
  23. Rescuing contaminated S. cerevisiae culture with antibiotics? - (reply: 1)
  24. Does kanR gene confer resistance to Ampicillin? - (reply: 1)
  25. cell lysate PAGE & blotting problem? - (reply: 1)
  26. ChIP Fixation & Protease inhibitors - (reply: 1)
  27. gel percentage & loading control for ATM and ATR? - western blotting (reply: 6)
  28. stable transfected cell line in absence of G418 - (reply: 6)
  29. colonies grow in amp plates not in broth - (reply: 6)
  30. Buffer Exchange & Protein Purification - (reply: 1)
  31. what to dissolve antibiotic in - (reply: 1)
  32. SDS-page & lipidated proteins - (reply: 6)
  33. cell harvesting; use of antibiotics? - (reply: 8)
  34. Soft Agar colony formation assay and G418 - (reply: 2)
  35. miRNA & mRNA microarray data correlation - is there any software that can correlate miRNA and mRNA data? (reply: 4)
  36. Reuse of LB Amp plates? - (reply: 7)
  37. ANTIBIOTIC RESISTANCE - (reply: 1)
  38. Ampicillin stability over 14 years - (reply: 5)
  39. Trypan blue & methylene blue - both to measure cell viability? (reply: 2)
  40. Zebrafish embryos time & temperature - (reply: 4)
  41. stability of ampicillin n kanamycin plates. - (reply: 4)
  42. Antibiotics in Cryoprotectant medium? - (reply: 3)
  43. can blunt & sticky ends stick - (reply: 11)
  44. G418 and hygromycin selection question - (reply: 3)
  45. Urgent amp agar plate question! - Help with stupid question (reply: 22)
  46. serum free culture - doubts about adaptation n antibiotics (reply: 6)
  47. co-transfection & transfection - (reply: 3)
  48. What to dissolve hygromycin and G418 with? - (reply: 3)
  49. intornic & intergenic EST? - (reply: 3)
  50. G418 and resistant cells - can't kill the cells (reply: 8)
  51. Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
  52. Confluence & Differentiation in PC-12 Cultures - How is Confluence in PC-12's Determined? Differentiation? (reply: 2)
  53. Differences between DGGE & standard electrophoresis? - (reply: 1)
  54. mutant protein expression & purification problem in E.coli - Protein with five residues mutated to Alanine's (reply: 6)
  55. PCR protocols - for some antibiotic resistant strains (reply: 2)
  56. G418 effect on protein concentration - (reply: 1)
  57. false positive clones URGENT - resitant to G418 but not expressing protein (reply: 13)
  58. G418 Killing curve - How to determinate the right G418 concentration by killing curve ? (reply: 5)
  59. Cells are not dying when I do the killing curve with G418 - (reply: 5)
  60. H&E staining - method (reply: 4)
  61. faint band of interest & primer-dimer prob - (reply: 5)
  62. G418 resistant 293 - (reply: 4)
  63. BigDye® v1.1 & v3.1 5X Sequencing Buffer Makeup? - (reply: 1)
  64. What is the right G418 concentration for selection on TSA and HEK? - (reply: 8)
  65. Can I use AMP plates with Agrobacterium? - (reply: 1)
  66. Stable transfection utilizing ampicillin resistance? - (reply: 2)
  67. Which antibiotic to choose? - puro vs hygro vs zeo etc (reply: 4)
  68. Kit for cyclic AMP assay - (reply: 2)
  69. A260/280 & A260/230 Parametrs for determining purity of a nucleic acid sampl - (reply: 4)
  70. Kanamycin in a F' strain? - problems with tn5? (reply: 2)
  71. Durability of pcr-machines - 4°C & peltier-elements (reply: 6)
  72. Kanamycin/Neomycin resistance - (reply: 3)
  73. antibiotic detection from mold - (reply: 1)
  74. forward & reverse primers - (reply: 3)
  75. G418 conc for HEK293 cells - (reply: 11)
  76. collagen H&E staining - tissue collagen staining (reply: 3)
  77. difference between RNA & cDNA gel image - (reply: 1)
  78. question about glacial acetic acid - glacial acetic acid & acetic acid (reply: 2)
  79. Precipitate in PCR buffer w/BSA & 2-mercaptoethanol - PCR buffers (reply: 1)
  80. Competant cells & incompetant scientist - No colonies formed (reply: 8)
  81. LC sample load & solubility advice? - Shimadzu prominence (reply: 4)
  82. amp v kan/neo resistance query - (reply: 3)
  83. Mutagenesis Help (plasmid isolation & sequencing stages) - (reply: 2)
  84. kanamycin activity with time - (reply: 4)
  85. Afl II linearization & ligation - (reply: 3)
  86. Homo & heterodimer Problem - (reply: 5)
  87. PCR amp. - sequencing problem - (reply: 3)
  88. Annexin staining - strange & mysterious population - (reply: 3)
  89. problem with culture revival & passage - (reply: 1)
  90. Problem with cell line/G418 - (reply: 8)
  91. G418 selection - (reply: 1)
  92. Slow growing cells (MCF-7 & MDA-MB231) - (reply: 7)
  93. Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
  94. Gel percentage & cross-linker percentage, protein MW constrains. - (reply: 2)
  95. Filtering & removing non-sequence data from a FASTA FILE using Perl scripts. - (reply: 9)
  96. Antibiotics and infection - (reply: 3)
  97. about G418 and neomycin - is there any difference? the solution? (reply: 10)
  98. stable transfectant - is there a way to make stables without antibiotic resistance? (reply: 2)
  99. Plate storage & picking colonies - How much time do I have? (reply: 4)
  100. Cells survived after G418 selection are transfected cells? - (reply: 2)
  101. Gel permeation & filtration - difference plz? (reply: 1)
  102. structure difference between plasmid & chromosomal DNA - (reply: 1)
  103. PCR my cDNA & got nothing - (reply: 2)
  104. cDNA precipitate & DNA ladders - (reply: 4)
  105. Did anyone here use neomycin to kill MCF-7 cells? - (reply: 4)
  106. about antibiotic in plasmid transformation - which is best? (reply: 6)
  107. who can tell me the valid period of ampicillin plate? - (reply: 3)
  108. Stable transfectants of HEK293 (selection with G418). Question about splitting ( - Cells growing confluent long before G418 kills them, is splitting smart or not? (reply: 9)
  109. Looking for a kanamycin cassette - (reply: 2)
  110. superoxide production & PMA - (reply: 1)
  111. sds page of protein in tfa & acotonitrile - (reply: 1)
  112. Unexpected antibiotics resistance - (reply: 4)
  113. Troubleshooting a Low 260/280 DNA ratio & Phenol storage - (reply: 2)
  114. Making ampicillin plates from plain LB plates - Making amp plates from prepoured LB plates (reply: 2)
  115. antibiotics light sensitivity - How sensitive are antibiotics really? (reply: 1)
  116. Sonication & RNA - (reply: 1)
  117. Problems cloning into pET Kanamycin resistance vectors - (reply: 7)
  118. Antibiotic marker expression in e.coli and geobacilli - (reply: 1)
  119. pcr - etbr & dna extract (reply: 2)
  120. Slit cells or add antibiotics, which is first after stable transfection - (reply: 8)
  121. G418 concentration - G418 concentration (reply: 5)
  122. Buying Pure Antibacterials/Antibiotics - Where Can I Buy Absolute Antibacterials/Antibiotics? (reply: 6)
  123. G418 vs Neomycin? - (reply: 8)
  124. nutrients & gene expression - (reply: 2)
  125. Direction of Amp cassette - (reply: 6)
  126. Which MSP & BGS primer design tool do you "swear" by? - (reply: 9)
  127. Methylation & PCR - (reply: 1)
  128. DNA & RNA Isolation - can any person give me protocol for this (reply: 4)
  129. antibiotic from actinomycetes - (reply: 2)
  130. Viral RNA extraction - Formalin fixed & Paraffin embended tissue (reply: 17)
  131. G418 Stable Transfection - (reply: 4)
  132. Pour plate & spread plate - Merits of pour plates (reply: 7)
  133. G418 in freezing medium? - (reply: 2)
  134. How much an H-bond can contribute to protein intergrity? - Protein structure & hydrogen bonds (reply: 2)
  135. RNA isolation - combination of Trizol & colum-based kit (reply: 6)
  136. MCF7 & MDA MB231 transfection - (reply: 3)
  137. antibiotics concentration for ligated plasmid - (reply: 3)
  138. plasmid extraction and antibiotics matters... - (reply: 4)
  139. antibiotics of bacillus subtilis - (reply: 2)
  140. Stable cell line - G418 - (reply: 5)
  141. antibiotics in medium-WHEN? - (reply: 10)
  142. Best time for adding G418? - transfection and selecting (reply: 2)
  143. Viral RNA & DNA extraction from serum - Protocols (reply: 1)
  144. AB resistance gene - Which antibiotic resistance gene in my vector ? (reply: 3)
  145. difference between LB Miller & LB, Lennox - difference between LB Miller & LB, Lennox (reply: 2)
  146. old degraded samples. DNA extraction & PCR - (reply: 3)
  147. How long time needed to determine the concentration of G418? - (reply: 2)
  148. Haematoxylin Eosin staining for frozen sections - H&E staining frozen sections (reply: 4)
  149. digestion & purification problem - (reply: 6)
  150. PCR Cloning - Please Help - Urgent - Cloning using SalI & BamHI site (reply: 1)
  151. PCR-Check, Screen-Check, MiniPrep - HUH?? - PCR product transformed, grown on AMP, no mini (reply: 2)
  152. Selection of clones during transfection - Concentration of G418 to be added to the cell. (reply: 7)
  153. How to create my own database and do local blast? - Local database & BLAST (reply: 2)
  154. Wester VS Immunoprecipitation -Da Paradigm...?!?! - Western Blot & Immunoprecipitaion & Ab (reply: 3)
  155. rRNA in insects - Information about 18S, 5.8S, ITS1 & ITS2 (reply: 1)
  156. Formalin fixation&paraffin-embedding of tissue culture cells - Formalin fixation&paraffin-embedding of tissue culture cells (reply: 1)
  157. Antibiotic working concentratios. - (reply: 2)
  158. G418 storage and contamination - (reply: 2)
  159. RNA & DNA extractionprotocols - (reply: 2)