What is the difference between double distiled water & RO water? - (Jun/28/2006 )
What is the difference between double distiled water & RO water? I want to use cell culture grade water to prepare my reagents for Skin tissue culture, What should i do? What kind of water should i use and how can i make it sterile for the purpose?
RO water means deionized water.
double distilled water is deionized water that have pass through many filters to remove impurity.
For cell culture use sterile double distilled water.
To sterile the double distilled water by autoclave or pass through a millipore filter.
I hope this may help.
we often come across three different terminologies fo rthe water we use.
milli RO, milli Q and Triple/ double / single distilled water.
RO is Reverse Osmosis while milli is for the company millipore which markets the technology as an equipment . water is purified based on reverse osmosis technique and is equivalant to Single distilled water.
milli Q water is based on exchange of ions by anion and cation exchanger. this gives usually high purity water with very less to negligible salt concentration and the conductivity is usually zero.
water purified by distillation (vapourization and condensation of the vapours so that salts are left in the container). the more no. of times you distill the water more is its purtity. usually triple distilled water has zero conductivity and is regarded as highly pure.
for tissue culturing you should use high purity water.
all the best
We use single distilled water which is then passed through MilliQ water sytem. We autoclave this water and use for preparation of cell culture reagents and media. Is this quality of water suitable for cell culture purpose?
BTW, we are facing some problem growing monocytic cultures, and likely reason is poor quality of water and media.
Can anybody comment?
Thanks in advance.