stability of ampicillin n kanamycin plates. - (Sep/21/2007 )
can anyone tell me for how long ampicillin, kanamycin, spectinomycin, streptomycin and hygromycin are stable in LB-agar plates? what is the optimal temperature of their storage?
Store them at 4ºC.
I usually seal them and kepp them stored for weeks (sometimes a couple of months...).
I've read that some people only trust them if they're stored for one or two weeks or so.
Different antibiotics have different stability, but I really don't know which are more and less stable... You can always ask the manufactor for some info.
well... i would not go more than 2 monthes.
Keep them inverted in bag at 4° to limit evaporation and degradation of antibiotics.
there is decline of antibiotics efficacy over the time (weeks);
after some weeks it is difficult to estimate the effective concentration; better use the plates freshly poured, or store plates without antibiotics and add them freshly before use; in this case you should use a fixed volume (f.i 20 ml of agar) to calculate what you have to add
thank you friends for suggestions...