G418 vs Neomycin? - (Oct/24/2005 )

This is probably a really dumb question , but I can't find the difference between G418 and neomycin. Does neomycin only select for the neo gene and G418 for the neo and others?

Thanks for your help!

hi
neomycine, geneticin and G418 are toxic for mammalian and bacterial cells except they carry the neomycin phospho transferase gene.

G418 also known as geneticin, is a broad-spectrum antibiotic that will select mammalian cells expressing the neomycin protein (encoded by the neomycin gene).

Neomycin will select bacteria expressing the same protein, but generally the promoter for Neo in mammalian expression plasmids is not usable in bacteria.

Hope this helps.

Thank you!!!!!

This is not written by me but I found it on some other forum. Just did a cut, copy, paste.

Cheers,
G.

neomycin-G418 confusion

'Mike' Michael J. Moser moser at U.WASHINGTON.EDU
Wed Oct 28 12:00:13 EST 1998
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Martin

Neomycin cannot be used to select mammalian cells. If it could it
wouldn't make a very good antibiotic for use in medicine. For example in
eye infection ointments the neomycin would kill the cells in your eye...
The confusion results from the fact that neomycin, kanamycin and G418 are
all similar compounds known as aminoglycosides that can be inhibited by
the product of the neo gene, neomycin phosphotransferase. G418 is
specific for the eukaryotic ribosome while the others more specifically
inhibit the bacterial organelle. I believe that G418 works in bacteria,
but at the price I'd rather use kanamycin. BTW be sure to use low salt LB
(5 g/l NaCl) or the kanamycin selection will wotk poorly if at all.

Good luck with it!

Mike Moser Tel: 206-543-6585
UW Department of Pathology FAX: 206-543-3967
Box 357705 moser at u.washington.edu
Seattle, WA 98195 http://weber.u.washington.edu/~moser

On Wed, 28 Oct 1998, Martin Offterdinger wrote:

> Hi everyone!
> There is some confusion about the antibiotics neomycin and G418 (The
> same as geneticin). In the Clontech catalogue you can read that a
> specific EGFPvector can be selected with kanamycin in bacteria and
> with neomycin in eukaryotic cells as it carries the kan/neo resistance
> gene. I therefore ordered Kanamycin and Neomycin as I want to generate
> a stable cell line. Now I realize that Neomycin seems to be
> ineffective in eukaryotic cells and that G418 has to be used for
> eukaryotic cells.
> You can imagine that I am quite angry about those guys from clontech,
> who put wrong informations into their catalogue.
> So my question is which information is correct: Can neomycin be used
> in eukaryotic( mammalian ) cells for selection purposes or not??? And
> : Why is there so much confusion about neo- resistance and G418
> selection?
> Martin
> Martin Offterdinger
> Internal Med.I,Dept. Oncology
> University of Vienna
> Austria
> E-Mail:a8803349.nospam at unet.univie.ac.at
> (remove .nospam before mailing)
>
>


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well. am trying to select my cells in g418 for almost 2 weeks now... they are growing happily at 1.6 mg/ml

well. am trying to select my cells in g418 for almost 2 weeks now... they are growing happily at 1.6 mg/ml

Just out of curiosity ... Why would a cell line I purchased from ATCC (Hep G2/2.2.1) in the information sheet suggest that I put G418 in the media? Is it just so it selects certain cells?

I hope this information can be useful for somebody:

"Transfected cells are also selected by resistence to a number of drugs, such as neomycin, its ANALOGUE Geneticin (G418), hygromycin, and methotrexate, by including a resistence-conferring gene in the construct used for transfection.

Culture in the correct concentration of the selective marker, determined by titration against the transfected and nontransfected controls, selects for stable transfectants."

---Freashney, R. Ian; Culture Of Animal Cells: A manual of basic technique. Fourth Edition. 2000. pp 208.

Saludos desde Mexico.
M. Alejandro C.
UABC- Laboratorio A-8 Biologia Molecular