excessive ampicillin in L.B/Amp plates - (Jul/16/2008 )
I accidently add excessive ampicillin to L.B and used that for making agar plates ( 800ul instead of 400ul in 400ml of L.. I need to used them for selection of HD alpha 5/pUC19, would it be a problem. Can I use these plates or should I make new ones ?
They will probably work. pUC19 is a high copy plasmid, and the amp resistance gene conveys high resistance. I'd definitely try them. You might want to run a control of plating out a known resistant strain to check that it grows, so that you will know if the plates are a problem, in the event of difficulties.
Either you can try it or just leave it for weeks and the amp will start to degrade. And then you can start to use them. but I am always very skeptical about the concentration.
So you doubled? But anyway, what is your end-concentration?
However, I do agree with phage434.
Thank you for reply, I am going to used them I hate waste.