Can I use AMP plates with Agrobacterium? - (Feb/08/2007 )
My Agrobacterium carries Rifampicin and Gentamicin resistance helper vector. My vector confers Ampicillin resistance. To grow this Agro and select the transformed with my construct, do I have to use Rif/Gent/Amp plates or Rif/Gent/Carbenicillin plates? I’ve heard that Agro must to be grown on Carbenicillin instead of Ampicillin. Why?
-dcgbio-
Carbenicillin is more stable compared to amp. And less heat sensitive compared to amp. And of couse carbenicillin is much more expensive though.
-timjim-