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Anyone use puromycin and blasticidin as selective Antibiotic for HeLa - (Oct/12/2005 )

I am trying to make stabe HELa cell line with transfected Psuperior vector or PCDNA6/TR vector.
The selective antibiotic are puromycin and blasticidin respectively. Any one did this kind of work before? How much is the concentration of the antibiotic?

Thank you guys very much!!!


for hela, i use 2µg/ml of puromycin. (for some guidelines see here)
i haven't personnaly use blasticidin, but here are the same guidelines.



Hi fred, Thank you very much for your information. It help me a lot.