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Real-time PCR Related Discussions
qPCR delta delta CT calculation -
(reply: 1)
DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression
(reply: 3)
mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages
(reply: 1)
qPCR troubles -
(reply: 5)
What grade of primers for real-time TaqMan PCR assays? -
(reply: 1)
qRT-PCR primer design -
(reply: 1)
Problems with second use of diluted cDNA in qPCR -
(reply: 4)
First strand cDNA synthesis for qRT-PCR -
(reply: 1)
Difference between semi-q and qPCR -
(reply: 2)
Stratagene MX4000 qPCR plates to give away - UK only & preferably pick up in Cambridge
(reply: 5)
qPCR internal control -
(reply: 3)
how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR
(reply: 6)
QPCR -
(reply: 9)
qPCR -
(reply: 1)
Low Ct value in ChIP qPCR -
(reply: 4)
Real-time PCR for mRNA stability -
(reply: 2)
qRT-PCR reproducibility of triplicates? -
(reply: 6)
How to get perfect amplification peaks - Share my long QPCR troubleshooting experience
(reply: 1)
qPCR standard curve problem -
(reply: 1)
how to optimize primer and cDNA concentration for real-time -
(reply: 2)
Using real-time to differentiate between SNPs -
(reply: 5)
Optimising Taqman probe - PCR is successful using SYBR but not with hydrolysis probe
(reply: 3)
qRT-PCR first timer, controls? -
(reply: 9)
ChIP and qPCR - ChIP and qPCR
(reply: 4)
Fluorescence level in real-time -
(reply: 2)
What are different ways of genotyping a transgenic mouse? - what is best way to do genotyping--PCR, qRT-PCR or RT-PCR
(reply: 3)
need help on microRNA real-time PCR -
(reply: 6)
TaqMan single v duplexing -
(reply: 5)
standard curve method for relative qPCR quantitation -
(reply: 2)
Primer concentration is quantitative Real-time PCR -
(reply: 1)
iQ5 dynamic well factors for TaqMan probes -
(reply: 5)
Real-Time PCR and Housekeeping gene -
(reply: 9)
Controls for real-time PCR... again -
(reply: 7)
Taqman PCR master mix -
(reply: 4)
help!real-time PCR to see gene's expression after treatment with cytokin -
(reply: 4)
taqman probe design -
(reply: 1)
Detection of LNA miRNA knock-down by qPCR? -
(reply: 5)
Taqman Vs SYBR Green - new to qRT-PCR and trying to learn....
(reply: 1)
SYBR green concentration for qPCR -
(reply: 2)
qrt-pcr on bacterial transcripts -
(reply: 1)
qPCR on low to non-existant template -
(reply: 4)
HELP with qPCR results -
(reply: 3)
realtime from different starting tissue masses -
(reply: 1)
Statistics: real-time RT-PCR -
(reply: 4)
Could you run Taqman PCR on gel to see the band? -
(reply: 1)
MULTIPLEX REAL-TIME PCR WITH SYBR-GREEN -
(reply: 1)
TaqMan dual-labelled probe low fluorescence problems -
(reply: 2)
urgent help-how to get amplification from very low target copy number and how to - "Taqman probe" qPCR and "rotor gene 6000" machine
(reply: 4)
qRT-PCR and Northern blot - Is miRNA Northern blot necessary?
(reply: 2)
No signal in Real-time but in normal PCR?! -
(reply: 2)
need help to evaluate primer sets for SYBR green qPCR -
(reply: 2)
qPCR databases -
(reply: 1)
QPcr for alternatively spliced products -
(reply: 4)
qPCR primer efficiancy -
(reply: 2)
Touchdown conditions for qPCR -
(reply: 1)
QPCR Quantitation of copy number -
(reply: 1)
ELISA in a real-time PCR plate - possible?
(reply: 3)
What different of TaqMan probe and Molecular Beacon probe ? - Why a reporter of TaqMan will be cleave, but not for a reporter of Mol
(reply: 2)
looking for a website for a newbie to learn qPCR -
(reply: 3)
Real-Time MSP -
(reply: 1)
qPCR housekeepers - primers or probes? -
(reply: 1)
what is CDS primer used as a control for QPCR in CHIP? -
(reply: 2)
Taqman or SYBR? -
(reply: 4)
real-time PCR products on gel look good but melting curve is horribel -
(reply: 2)
Free FastStart Universal Real-Time PCR Master Mixes from Roche -
(reply: 7)
Real-time PCR....Making sense of it all? -
(reply: 3)
QPCR with MSP -
(reply: 3)
Homemade TaqMan PCR Mastermix? -
(reply: 1)
Verification of real-time amplification specificity -
(reply: 1)
miRNA QPCR advice - taqman
(reply: 3)
No-RT control in qRT-PCR -
(reply: 5)
Longest Amplicon Using Real-Time PCR? -
(reply: 1)
cDNA for real-time PCR -
(reply: 3)
PCR - qPCR inconsistent - To explain differences between normal PCR and qPCR
(reply: 6)
One-step versus two-step qRT-PCR -
(reply: 5)
primer for real-time and reverse transcriptase PCR -
(reply: 1)
Sybergreen vs taqman? - Comparable?
(reply: 1)
Plasmid for qPCR standard curve -
(reply: 3)
Efficiency greater than 100% using taqman probes? -
(reply: 4)
Target Amplicon Length for real-time PCR -
(reply: 4)
can Primers for RT-PCR be used Real-Time PCR directly? -
(reply: 2)
need advice on qPCR results (see attachment) - melting curve - gel picture
(reply: 5)
SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt
(reply: 1)
highly recommended company or etc for TaqMan probe in MethyLight -
(reply: 3)
RealTime PCR of microRNAs -
(reply: 9)
Bad slopes in TaqMan reactions -
(reply: 3)
How can I prepare standard DNA for QPCR -
(reply: 3)
qPCR Analysis - Standard Error of Individual dCT vs Group average expression
(reply: 4)
Pre-Preparation of qPCR plates - Pros and Cons
(reply: 4)
Re-amplify PCR product by realtime PCR? -
(reply: 13)
Trouble with real-time PCR standard curve -
(reply: 4)
qPCR with Corbett Rotor Gene 6000 -
(reply: 3)
mirVana qRT-PCR with the Roche 1.5 LightCycler -
(reply: 1)
NTC's in RT or just qPCR? -
(reply: 3)
qPCR Efficiencies over 100% - Looking for references
(reply: 1)
qPCR, no RT controls always positive (as cDNA samples) -
(reply: 4)
TAQMAN real time - what is this???
(reply: 4)
Plate setup- have I covered everything? - help- qRT-PCR newbie in state of confusion!
(reply: 2)
Series dilution of RNA or cDNA for qPCR standard curve? -
(reply: 1)
random primers for qPCR -
(reply: 3)
qPCR standards -
(reply: 1)
TaqMan probes vs Molecular Beacon probes -
(reply: 1)
RT-qPCR of bacteria mRNA from sludge - RT-PCR, Real-time PCR, mRNA, sludge
(reply: 3)
qPCR detection for single nucleotide substitution -
(reply: 5)
taqman assays and linreg -
(reply: 1)
Which housekeeping genes for qRT-PCR control? -
(reply: 17)
generation of gDNA Std curves in qPCR - ABI 7300 qPCR
(reply: 1)
is cDNA concentration 200 ng/ul too high for qRT-PCR -
(reply: 17)
What's the function of UNG in qPCR? -
(reply: 6)
ChIP quantification (qPCR) -
(reply: 2)
Self-made qRT-PCR enzyme-/mastermix? -
(reply: 8)
Home-made Sybr green mix for real-time PCR -
(reply: 1)
Real-time PCR problems - RT_PCR
(reply: 7)
Homemade RealTime Syb-GreenI-Mix -
(reply: 23)
Problem with QPCR - control gene does not amplify -
(reply: 2)
Smeary bands in real-time RT-PCR -
(reply: 7)
real-time slopes -
(reply: 4)
1st time real-time PCR questions -
(reply: 1)
Optimal size for qRt-PCR -
(reply: 3)
Primer Dimer problems in real-time PCR -
(reply: 14)
some qPCR questions (naive ones included)... - first pitfals etc...
(reply: 8)
designing QRT-PCR Primers -
(reply: 11)
Another QPCR problem. Please help -
(reply: 5)
Real-time PCR machines for DNA quantitation -
(reply: 5)
TaqMan probes, pcr mix -
(reply: 1)
strange QPCR amplificaton plot -
(reply: 3)
Mg curve for real-time PCR - Strange result
(reply: 5)
Real-time PCR probe degradation - when do they degrades?
(reply: 4)
My SYBRgreen qPCR stopped working -
(reply: 3)
real-time thresholds - purpose? -
(reply: 1)
real-time PCR - Multiple melting curve peaks
(reply: 18)
Real-time PCR questions (Mx3000P) - Need some help with interpretation
(reply: 7)
qRT-PCR data analysis -
(reply: 1)
mastermix for Real-time PCR -
(reply: 2)
qPCR standard curves - how can I keep the quality good?
(reply: 6)
real-time PCR -
(reply: 5)
Real-time PCR amplification curve up and down -
(reply: 3)
Do I have amplication of RNA by qPCR? -
(reply: 7)
real-time RT-PCR one cell -
(reply: 2)
Maximum copy number in Taqman -
(reply: 1)
QRT-PCR -
(reply: 4)
Shopping for real-time PCR thermal cyclers - iCycleriQ real-time system?
(reply: 1)
Real-time PCR problems -
(reply: 3)
real-time PCR/ABI Prism 7700 - Std curve programming
(reply: 1)
realtime RT-PCR/internal control -
(reply: 3)
product size for real-time PCR primer -
(reply: 3)
Real-time quantification in Chromatin IP -
(reply: 2)
Taqman SNP genotyping - Reduced reaction volume
(reply: 1)
Transcription Rate - Measure Hn RNA using Taqman??
(reply: 1)
does anyone know real-time methyaltion pcr? -
(reply: 1)
real-time PCR - How to design primers
(reply: 2)
QRT-PCR result analysis - relative transcript level?
(reply: 3)
Probes for Real-Time PCR -
(reply: 3)