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Real-time PCR Related Discussions
  1. qPCR delta delta CT calculation - (reply: 1)
  2. DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression (reply: 3)
  3. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
  4. qPCR troubles - (reply: 5)
  5. What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
  6. qRT-PCR primer design - (reply: 1)
  7. Problems with second use of diluted cDNA in qPCR - (reply: 4)
  8. First strand cDNA synthesis for qRT-PCR - (reply: 1)
  9. Difference between semi-q and qPCR - (reply: 2)
  10. Stratagene MX4000 qPCR plates to give away - UK only & preferably pick up in Cambridge (reply: 5)
  11. qPCR internal control - (reply: 3)
  12. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 6)
  13. QPCR - (reply: 9)
  14. qPCR - (reply: 1)
  15. Low Ct value in ChIP qPCR - (reply: 4)
  16. Real-time PCR for mRNA stability - (reply: 2)
  17. qRT-PCR reproducibility of triplicates? - (reply: 6)
  18. How to get perfect amplification peaks - Share my long QPCR troubleshooting experience (reply: 1)
  19. qPCR standard curve problem - (reply: 1)
  20. how to optimize primer and cDNA concentration for real-time - (reply: 2)
  21. Using real-time to differentiate between SNPs - (reply: 5)
  22. Optimising Taqman probe - PCR is successful using SYBR but not with hydrolysis probe (reply: 3)
  23. qRT-PCR first timer, controls? - (reply: 9)
  24. ChIP and qPCR - ChIP and qPCR (reply: 4)
  25. Fluorescence level in real-time - (reply: 2)
  26. What are different ways of genotyping a transgenic mouse? - what is best way to do genotyping--PCR, qRT-PCR or RT-PCR (reply: 3)
  27. need help on microRNA real-time PCR - (reply: 6)
  28. TaqMan single v duplexing - (reply: 5)
  29. standard curve method for relative qPCR quantitation - (reply: 2)
  30. Primer concentration is quantitative Real-time PCR - (reply: 1)
  31. iQ5 dynamic well factors for TaqMan probes - (reply: 5)
  32. Real-Time PCR and Housekeeping gene - (reply: 9)
  33. Controls for real-time PCR... again - (reply: 7)
  34. Taqman PCR master mix - (reply: 4)
  35. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
  36. taqman probe design - (reply: 1)
  37. Detection of LNA miRNA knock-down by qPCR? - (reply: 5)
  38. Taqman Vs SYBR Green - new to qRT-PCR and trying to learn.... (reply: 1)
  39. SYBR green concentration for qPCR - (reply: 2)
  40. qrt-pcr on bacterial transcripts - (reply: 1)
  41. qPCR on low to non-existant template - (reply: 4)
  42. HELP with qPCR results - (reply: 3)
  43. realtime from different starting tissue masses - (reply: 1)
  44. Statistics: real-time RT-PCR - (reply: 4)
  45. Could you run Taqman PCR on gel to see the band? - (reply: 1)
  46. MULTIPLEX REAL-TIME PCR WITH SYBR-GREEN - (reply: 1)
  47. TaqMan dual-labelled probe low fluorescence problems - (reply: 2)
  48. urgent help-how to get amplification from very low target copy number and how to - "Taqman probe" qPCR and "rotor gene 6000" machine (reply: 4)
  49. qRT-PCR and Northern blot - Is miRNA Northern blot necessary? (reply: 2)
  50. No signal in Real-time but in normal PCR?! - (reply: 2)
  51. need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
  52. qPCR databases - (reply: 1)
  53. QPcr for alternatively spliced products - (reply: 4)
  54. qPCR primer efficiancy - (reply: 2)
  55. Touchdown conditions for qPCR - (reply: 1)
  56. QPCR Quantitation of copy number - (reply: 1)
  57. ELISA in a real-time PCR plate - possible? (reply: 3)
  58. What different of TaqMan probe and Molecular Beacon probe ? - Why a reporter of TaqMan will be cleave, but not for a reporter of Mol (reply: 2)
  59. looking for a website for a newbie to learn qPCR - (reply: 3)
  60. Real-Time MSP - (reply: 1)
  61. qPCR housekeepers - primers or probes? - (reply: 1)
  62. what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
  63. Taqman or SYBR? - (reply: 4)
  64. real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
  65. Free FastStart Universal Real-Time PCR Master Mixes from Roche - (reply: 7)
  66. Real-time PCR....Making sense of it all? - (reply: 3)
  67. QPCR with MSP - (reply: 3)
  68. Homemade TaqMan PCR Mastermix? - (reply: 1)
  69. Verification of real-time amplification specificity - (reply: 1)
  70. miRNA QPCR advice - taqman (reply: 3)
  71. No-RT control in qRT-PCR - (reply: 5)
  72. Longest Amplicon Using Real-Time PCR? - (reply: 1)
  73. cDNA for real-time PCR - (reply: 3)
  74. PCR - qPCR inconsistent - To explain differences between normal PCR and qPCR (reply: 6)
  75. One-step versus two-step qRT-PCR - (reply: 5)
  76. primer for real-time and reverse transcriptase PCR - (reply: 1)
  77. Sybergreen vs taqman? - Comparable? (reply: 1)
  78. Plasmid for qPCR standard curve - (reply: 3)
  79. Efficiency greater than 100% using taqman probes? - (reply: 4)
  80. Target Amplicon Length for real-time PCR - (reply: 4)
  81. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
  82. need advice on qPCR results (see attachment) - melting curve - gel picture (reply: 5)
  83. SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
  84. highly recommended company or etc for TaqMan probe in MethyLight - (reply: 3)
  85. RealTime PCR of microRNAs - (reply: 9)
  86. Bad slopes in TaqMan reactions - (reply: 3)
  87. How can I prepare standard DNA for QPCR - (reply: 3)
  88. qPCR Analysis - Standard Error of Individual dCT vs Group average expression (reply: 4)
  89. Pre-Preparation of qPCR plates - Pros and Cons (reply: 4)
  90. Re-amplify PCR product by realtime PCR? - (reply: 13)
  91. Trouble with real-time PCR standard curve - (reply: 4)
  92. qPCR with Corbett Rotor Gene 6000 - (reply: 3)
  93. mirVana qRT-PCR with the Roche 1.5 LightCycler - (reply: 1)
  94. NTC's in RT or just qPCR? - (reply: 3)
  95. qPCR Efficiencies over 100% - Looking for references (reply: 1)
  96. qPCR, no RT controls always positive (as cDNA samples) - (reply: 4)
  97. TAQMAN real time - what is this??? (reply: 4)
  98. Plate setup- have I covered everything? - help- qRT-PCR newbie in state of confusion! (reply: 2)
  99. Series dilution of RNA or cDNA for qPCR standard curve? - (reply: 1)
  100. random primers for qPCR - (reply: 3)
  101. qPCR standards - (reply: 1)
  102. TaqMan probes vs Molecular Beacon probes - (reply: 1)
  103. RT-qPCR of bacteria mRNA from sludge - RT-PCR, Real-time PCR, mRNA, sludge (reply: 3)
  104. qPCR detection for single nucleotide substitution - (reply: 5)
  105. taqman assays and linreg - (reply: 1)
  106. Which housekeeping genes for qRT-PCR control? - (reply: 17)
  107. generation of gDNA Std curves in qPCR - ABI 7300 qPCR (reply: 1)
  108. is cDNA concentration 200 ng/ul too high for qRT-PCR - (reply: 17)
  109. What's the function of UNG in qPCR? - (reply: 6)
  110. ChIP quantification (qPCR) - (reply: 2)
  111. Self-made qRT-PCR enzyme-/mastermix? - (reply: 8)
  112. Home-made Sybr green mix for real-time PCR - (reply: 1)
  113. Real-time PCR problems - RT_PCR (reply: 7)
  114. Homemade RealTime Syb-GreenI-Mix - (reply: 23)
  115. Problem with QPCR - control gene does not amplify - (reply: 2)
  116. Smeary bands in real-time RT-PCR - (reply: 7)
  117. real-time slopes - (reply: 4)
  118. 1st time real-time PCR questions - (reply: 1)
  119. Optimal size for qRt-PCR - (reply: 3)
  120. Primer Dimer problems in real-time PCR - (reply: 14)
  121. some qPCR questions (naive ones included)... - first pitfals etc... (reply: 8)
  122. designing QRT-PCR Primers - (reply: 11)
  123. Another QPCR problem. Please help - (reply: 5)
  124. Real-time PCR machines for DNA quantitation - (reply: 5)
  125. TaqMan probes, pcr mix - (reply: 1)
  126. strange QPCR amplificaton plot - (reply: 3)
  127. Mg curve for real-time PCR - Strange result (reply: 5)
  128. Real-time PCR probe degradation - when do they degrades? (reply: 4)
  129. My SYBRgreen qPCR stopped working - (reply: 3)
  130. real-time thresholds - purpose? - (reply: 1)
  131. real-time PCR - Multiple melting curve peaks (reply: 18)
  132. Real-time PCR questions (Mx3000P) - Need some help with interpretation (reply: 7)
  133. qRT-PCR data analysis - (reply: 1)
  134. mastermix for Real-time PCR - (reply: 2)
  135. qPCR standard curves - how can I keep the quality good? (reply: 6)
  136. real-time PCR - (reply: 5)
  137. Real-time PCR amplification curve up and down - (reply: 3)
  138. Do I have amplication of RNA by qPCR? - (reply: 7)
  139. real-time RT-PCR one cell - (reply: 2)
  140. Maximum copy number in Taqman - (reply: 1)
  141. QRT-PCR - (reply: 4)
  142. Shopping for real-time PCR thermal cyclers - iCycleriQ real-time system? (reply: 1)
  143. Real-time PCR problems - (reply: 3)
  144. real-time PCR/ABI Prism 7700 - Std curve programming (reply: 1)
  145. realtime RT-PCR/internal control - (reply: 3)
  146. product size for real-time PCR primer - (reply: 3)
  147. Real-time quantification in Chromatin IP - (reply: 2)
  148. Taqman SNP genotyping - Reduced reaction volume (reply: 1)
  149. Transcription Rate - Measure Hn RNA using Taqman?? (reply: 1)
  150. does anyone know real-time methyaltion pcr? - (reply: 1)
  151. real-time PCR - How to design primers (reply: 2)
  152. QRT-PCR result analysis - relative transcript level? (reply: 3)
  153. Probes for Real-Time PCR - (reply: 3)