qRT-PCR for pre- and pri-miRNAs - (Nov/23/2008 )
I was wondering if anyone knows how to do qRT-PCR with pre- and pri-miRNAs. The kits for mature miRNAs are available, but not for pri- and pre-. I've read about several papers doing that, but not sure about what is the general and validated way of doing qRT-PCRs for pre- and/or pri-miRNAs...
You can design primers targeting pri- and pri/pre-miRNAs for qRT-PCR.
i did it for 1 mirna....it seem to work but remember you have to fight against an hairpin!! primer design is tricky!