qPCR - (Aug/05/2008 )
I would like to ask a general question about measuring the expression levels of a certain gene.
Why cant we just measure the mRNA extracted from the cells instead doing another step by converting to cDNA?
it will be time saving and less erorr posibilities or?
Don't they have a special detector that can bind to single stranded mRNA instead of syber green that binds to double stranded DNA?
RNA is very unstable and degrates "just for fun", that why we do so much work and convert it to cDNA. DNA is more stable (that's why the dino dna can be study). So sorry no short cuts...