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looking for a website for a newbie to learn qPCR - (Jul/11/2007 )

Hi,

My boss is pushing me for sybr qPCR after I got some good pilot data from tranditional end point semi-quantitative RT-PCR. But I am new to real time PCR and I heared that sybr qPCR needs considerable experience to analyze the data. So, I am wondering there is a website for me to learn to anyalyze sybr qPCR. for example, what are the most important values and how does it work.

-flakie-

QUOTE (flakie @ Jul 11 2007, 10:49 AM)
Hi,

My boss is pushing me for sybr qPCR after I got some good pilot data from tranditional end point semi-quantitative RT-PCR. But I am new to real time PCR and I heared that sybr qPCR needs considerable experience to analyze the data. So, I am wondering there is a website for me to learn to anyalyze sybr qPCR. for example, what are the most important values and how does it work.


good one for learning the quantitative part
http://www.gene-quantification.de/download.html#passwd
the practical part is relatively easy...just like seeting up normal PCRs ,..just that it might be many more samples

-Watson-

I found this site very useful.

K.

-kr├╝melmonster-

Hi,

I just found this website that I thought was really usefull. It's more about how to analyze your data.

http://www.rci.rutgers.edu/~gray/RealtimeP...lysis_guide.htm

Good luck!

-Ddkb-