looking for a website for a newbie to learn qPCR - (Jul/11/2007 )
Hi,
My boss is pushing me for sybr qPCR after I got some good pilot data from tranditional end point semi-quantitative RT-PCR. But I am new to real time PCR and I heared that sybr qPCR needs considerable experience to analyze the data. So, I am wondering there is a website for me to learn to anyalyze sybr qPCR. for example, what are the most important values and how does it work.
-flakie-
QUOTE (flakie @ Jul 11 2007, 10:49 AM)
Hi,
My boss is pushing me for sybr qPCR after I got some good pilot data from tranditional end point semi-quantitative RT-PCR. But I am new to real time PCR and I heared that sybr qPCR needs considerable experience to analyze the data. So, I am wondering there is a website for me to learn to anyalyze sybr qPCR. for example, what are the most important values and how does it work.
My boss is pushing me for sybr qPCR after I got some good pilot data from tranditional end point semi-quantitative RT-PCR. But I am new to real time PCR and I heared that sybr qPCR needs considerable experience to analyze the data. So, I am wondering there is a website for me to learn to anyalyze sybr qPCR. for example, what are the most important values and how does it work.
good one for learning the quantitative part
http://www.gene-quantification.de/download.html#passwd
the practical part is relatively easy...just like seeting up normal PCRs ,..just that it might be many more samples
-Watson-
I found this site very useful.
K.
-krümelmonster-
Hi,
I just found this website that I thought was really usefull. It's more about how to analyze your data.
http://www.rci.rutgers.edu/~gray/RealtimeP...lysis_guide.htm
Good luck!
-Ddkb-