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Help! Opticon real-time machines thinks I only want to do 1 cycle! - (Sep/18/2006 )

Hi all,

I am using the DNA engine Opticon 2 from MJ research. I was performing some PCRs and the first one I did ran to 50 cycles like I wanted. However, immediately after I ran another one that I wanted to go to 30 cycles. I loaded my different protocol and started it up.

I came back to it a little while later and found that it only had gone to 1 cycle!

We have had a problem with in the past where it faulted and stopped at random cycle numbers, however this was different. The machine/software didnt pick up that I wanted 30 cycles even at the start. And on the status screen only said running 1 of 1 cycle, instead of its usual 1 of 30 cycles.

I tried loading a different protocol, still the same. I tried writing a fresh one and still it happened.

Rebooting also didn't work.

I am now running a virus scan, but I don't think that will work either.

Has anyone else experienced this problem. What should I do? Should I maybe re-install software?
Or is time to contact the company? Although I heard they may have gone bust?

Please help, any ideas truely appreciated.

Thanks

-DrEmma-

It amazes me that people don't contact the comany immediately when this kind of thing happens.

Get on the phone ASAP!

-John Buckels-

QUOTE (John Buckels @ Sep 18 2006, 06:34 PM)
It amazes me that people don't contact the comany immediately when this kind of thing happens.

Get on the phone ASAP!



Yeah thanks - really helpful! Like I said the company we bought it from no longer exist, however, we are onto another which may be able to help.

-DrEmma-

Hi,
MJ machines are now taken over by BioRad. Just contact them. Maybe you get the newest software?!
Stay cool,
thb.

-thb-