Taqman SNP genotyping - Reduced reaction volume (Oct/08/2004 )
How low is it possible to reduce the reaction volume to and still get accurate SNP genotypes - using a 7900HT? I'm looking for ways to reduce costs! Also, is it possible to multiplex using this system?
multiplex Taqman is possible, but difficult.
You can reduce the reaction volume, I think we have reduced a normal 25 ul to 10 without problems.
Just try it..