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Taqman SNP genotyping - Reduced reaction volume (Oct/08/2004 )

How low is it possible to reduce the reaction volume to and still get accurate SNP genotypes - using a 7900HT? I'm looking for ways to reduce costs! Also, is it possible to multiplex using this system?




multiplex Taqman is possible, but difficult.

You can reduce the reaction volume, I think we have reduced a normal 25 ul to 10 without problems.
Just try it..