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Medium Related Discussions
  1. Bacterial Media Formulation - (reply: 2)
  2. Preparation of Rich Medium for cultivation of Zymomonas sp - (reply: 3)
  3. Addition of antibiotic to the culture medium - (reply: 1)
  4. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
  5. Pelleting cells by gentle centrifugation for addition to media - (reply: 13)
  6. Media doesn't change colour? - (reply: 4)
  7. Stromal conditioned medium (SCM) - (reply: 1)
  8. Pore formation in cells after adding amphotericin B in culture medium - (reply: 2)
  9. Injection of human cell culture conditioned media into nude mice - (reply: 1)
  10. Can I make an SDS stock in media and store? - (reply: 3)
  11. Quick question about making culture media. - (reply: 5)
  12. Effect of low and high nutrient medium on bacterial attachment? - (reply: 1)
  13. conditioned medium for ischaemia study - (reply: 1)
  14. different type of media for cell culture - (reply: 1)
  15. Can medium containing starch be autoclaved? - (reply: 36)
  16. Preparing a conditioned medium from cancer cells - (reply: 3)
  17. Adjusting pH of RPMI/ bicarbonate containing media - (reply: 9)
  18. Help with media filtration - (reply: 5)
  19. Lentiviral media - HEPES buffered DMEM - do I titrate? - (reply: 1)
  20. Purpose Of Fatty Acids In Media - (reply: 1)
  21. Help w/ Media Preparation - (reply: 3)
  22. How long Kanamycin can last in broth medium? - (reply: 3)
  23. Taking Culture Medium Samples for LDH Assay - (reply: 2)
  24. Increasing cell growth rate in plain M9 medium - (reply: 1)
  25. culture media for pancreatic cancer stem cells - (reply: 2)
  26. Use-by-date of antibiotic & serum in media - (reply: 6)
  27. E. coli growing on plates and bot not in LB medium - (reply: 10)
  28. Looking for Staib agar dehydrated medium - (reply: 3)
  29. precipitation of copper in minimal medium - (reply: 5)
  30. What will happen if the cell culture medium is suddenly changed to another one? - (reply: 2)
  31. Help, I accidently warmed my media componemts to 57ºC! - (reply: 3)
  32. Reagent used to adjust pH of cell culture media - (reply: 3)
  33. Powder indomethacin in cell culture media?? - (reply: 2)
  34. Can i still use freezing medium if i used PES filter to filter my DMSO? - (reply: 1)
  35. Precipitating growth medium - (reply: 2)
  36. Not sure how to make HEPES or MOPS buffered media - (reply: 6)
  37. Pichia growth in MGY/MGYH media - (reply: 1)
  38. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
  39. Left my media at room temperature for 48 hours; okay to use? - (reply: 4)
  40. MEDIA REDOX STATUS - (reply: 3)
  41. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
  42. media supplements - (reply: 2)
  43. Culture media for BGMK-hDAF - (reply: 3)
  44. Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
  45. Elisa using HEK culture medium - (reply: 14)
  46. changing cell culture media - (reply: 1)
  47. Determining whether media contains serum or not? - (reply: 5)
  48. Retrovirus production & media color (pH) - (reply: 3)
  49. Calculating the amount of the desired cells in the medium ? - (reply: 3)
  50. How long do growth factors last in complete media? - (reply: 3)
  51. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
  52. yeast growth in SC-ura medium - (reply: 1)
  53. miRNA extraction from cell-conditioned media? - (reply: 1)
  54. Pseudomonas aeruginosa growth very slow in N-minimal media - (reply: 2)
  55. Microalgae media - pH adjustment - (reply: 1)
  56. cells shrink, detach from each other when changing media - (reply: 3)
  57. Preparation of Conditioned Medium - (reply: 1)
  58. cell culture media refrigeration - (reply: 4)
  59. ph? when i should corect the ph of media? - (reply: 6)
  60. Extrct RNA from fungus in broth medium - (reply: 2)
  61. 5-FOA media not letting ANY cells grow... help! - (reply: 1)
  62. SUCROSE AS FRACTIONATION MEDIUM - (reply: 1)
  63. Adjusting pH of cell culture medium for ELISA - (reply: 1)
  64. testing media sterility - (reply: 2)
  65. Using a different media than recommended - (reply: 1)
  66. Medium formulation - (reply: 2)
  67. problems on the media for oryza sativa(rice) - (reply: 2)
  68. Freezing culture media - (reply: 4)
  69. antibiotics in media - (reply: 2)
  70. Preparing media with different pHs - (reply: 1)
  71. RPMI 1640 media - (reply: 2)
  72. Growing hybridoma cells in medium without serum - (reply: 2)
  73. Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
  74. Minimum salt medium preparation - (reply: 1)
  75. Expired media - How long after the expiration data is cell culture media good? (reply: 2)
  76. Live cell Imaging medium - (reply: 1)
  77. Concentrated conditioned medium for western blot - (reply: 1)
  78. Filter Sterilization of Hot Gellan Gum Media - (reply: 3)
  79. problems with MTT assay (some formazan crystals in the medium) - (reply: 1)
  80. E.coli cells lyse in minimal media - (reply: 3)
  81. cell suspencion and medium - (reply: 5)
  82. Cloudy/Turbid media after subculture - (reply: 3)
  83. Autoclave before adding MgCl2 and MgSO4? - SOB medium receipt (reply: 4)
  84. Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
  85. siRNA transfection - 7-day study - serum free media? (reply: 2)
  86. MTT assay medium with phenol red - (reply: 2)
  87. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
  88. cell culture media - glutamine! - (reply: 2)
  89. M9 medium??? - (reply: 1)
  90. Media - a beautiful pink! - NaOH in media (reply: 3)
  91. IF coverslipping slides / mounting medium / sealer - (reply: 2)
  92. Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
  93. Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
  94. Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
  95. Insulin supplements to cell culture media - (reply: 1)
  96. growth of E.coli in minimal media(m9) is very less - growth of E.coli in minimal media(m9) is very less (reply: 2)
  97. Running a western with cell media in gels? - (reply: 11)
  98. Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
  99. Yeast Minimal GS Medium Recipe? - (reply: 6)
  100. Preparing M&S medium - (reply: 4)
  101. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
  102. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
  103. Anitbiotic shelf life in liquid media at RT - (reply: 2)
  104. problems with 293T cells after medium removal!!! - (reply: 2)
  105. Enough media? - (reply: 1)
  106. recombinant ecoli in log phase overnight media suggestion - (reply: 2)
  107. cell culture media - (reply: 3)
  108. Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
  109. question - glucose additin to media (reply: 2)
  110. problem in M9 media - m9 media (reply: 4)
  111. Need pH 6 Cell culture media - (reply: 1)
  112. floating THP-1 cells - medium change advice - (reply: 2)
  113. Culture Media for stem cells - (reply: 1)
  114. Can buffering strength of media reduce with time ? - (reply: 4)
  115. Help!my 293T stop growth and become slim 24h after 1:3 splitting - unless you change new medium for them 24h after splitting (reply: 3)
  116. Keeping media at -80C - (reply: 3)
  117. Why does my media turn alkaline not acidic? - MCF-7 in EMEM (reply: 4)
  118. Concerning Cultivation of Monoclonal Antibodies - How long before antibodies spoil in media? (reply: 1)
  119. I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
  120. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
  121. Change of colour in cell culture media...? - (reply: 2)
  122. Adding L-glutamine to DMEM media w/o L-glutamine - (reply: 5)
  123. Bacteria Media (NZY+ broth) - (reply: 1)
  124. Mounting Medium for DIG in situ - (reply: 1)
  125. Bacteria growing on plate but not liquid media - (reply: 8)
  126. Trypsin/EDTA inactivation - which compound in the medium is responsible for inactivation? (reply: 5)
  127. what type of media for gingiva fibroblast tissue culture? - (reply: 3)
  128. too much nahco3 in medium - (reply: 1)
  129. HOW LONG CAN I STORE MY MEDIA...? - (reply: 3)
  130. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
  131. Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
  132. Can I IP from media? - to use for SDS-PAGE? (reply: 1)
  133. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
  134. satbility of DMEM media - (reply: 1)
  135. Why does medium turn yellow fast during the intensive synthesis of exogenous pro - Is it the by-product of metabolism?Is it lactic acid? (reply: 2)
  136. Half life o acetaminophen - on culture media (reply: 1)
  137. Sodium pyruvate in medium - (reply: 1)
  138. precipitation of secreted protein from growth media - (reply: 1)
  139. What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
  140. Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
  141. Cellular proteins in medium - (reply: 1)
  142. how long to autoclave the medium - (reply: 2)
  143. western blot using culture media - (reply: 4)
  144. Storage of Freezing Media - (reply: 2)
  145. Hoe much media needed for transfection complex? - transfection (reply: 9)
  146. low serum media and FCS - (reply: 2)
  147. Cell culture media - require information on media (reply: 3)
  148. Freezing cells- Media or FBS ? - (reply: 2)
  149. phenol red in media - (reply: 5)
  150. LB and SB electrophoresis media - (reply: 1)
  151. Daily medium change - (reply: 3)
  152. Calcium Phosphate transfection help - cells detaching after media change (reply: 1)
  153. My cells died after changing media... - (reply: 6)
  154. culture medium sterilization - (reply: 3)
  155. NSC-34 Cells sloughing off at media change - (reply: 4)
  156. which medium to use in cocultures - (reply: 3)
  157. Critical column of media above cells for access of oxygen - (reply: 1)
  158. question about media color - (reply: 4)
  159. adding bicarbonate to medium...help please! - (reply: 1)
  160. transformation medium - (reply: 2)
  161. How much cell medium for 1 week of culturing? - (reply: 1)
  162. adding fungicidies to the media in plant tissue culture - (reply: 2)
  163. Basal Media preparation - (reply: 2)
  164. iPS media - (reply: 1)
  165. Conditioned media preparation - (reply: 3)
  166. Subculture in low glucose media - (reply: 2)
  167. Cells suddenly died after changing the medium... why? - (reply: 4)
  168. DMEM vs RPMI for primary mouse lymphocytes - Which media for primary mouse lymphocyte? (reply: 2)
  169. New Gibco Media bottles - (reply: 1)
  170. Alternative media for PC-12 cells - (reply: 2)
  171. cho cell line adoptation to serum free medium - (reply: 1)
  172. antibiotic stability in culture medium - (reply: 1)
  173. Serum free culture media 293T cells? - (reply: 1)
  174. How long do you warm up media? - (reply: 9)
  175. Protein purification from Insect media - (reply: 1)
  176. conditioned medium - how to prepare conditioned media (reply: 2)
  177. Cell culture - Culture medium (reply: 1)
  178. Media for squamous cells - (reply: 3)
  179. Oxygen diffusion through cell culture medium - (reply: 2)
  180. Conditioned media question - (reply: 2)
  181. huvec change medium?? - (reply: 4)
  182. opti-mem as a serum starvation media - (reply: 1)
  183. 2,4-D substitute for Gamborg's B5 medium??? - (reply: 2)
  184. 5% vs 10% CO2 (with their corresponding media) - How relevant it is? (reply: 5)
  185. Transfection problem - milky media - (reply: 1)
  186. aliquot media recude contamination? - (reply: 3)
  187. Isolation of secreted proteins from culture media - (reply: 2)
  188. Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
  189. Media prep question - (reply: 6)
  190. vogel mineral growth media for fungi - (reply: 1)
  191. Storing conditioned media - (reply: 3)
  192. Cytotoxicity of hydrophobic substances - How to dissolve highly hydrophobic substances in aqueus growth medium? (reply: 2)
  193. Semisolid Medium For HL 60 cells - (reply: 4)
  194. Problem Maintaining pH in Culture Media - (reply: 4)
  195. NPB medium - (reply: 3)
  196. L.Lactis culture - M17 media (reply: 6)
  197. expression in minimal media - (reply: 14)
  198. media - (reply: 2)
  199. Treating cells with chemicals and how to dilute into medium - (reply: 5)
  200. Chromogenic Media - (reply: 1)
  201. Wnt 3a conditional medium - (reply: 1)
  202. Shelf life of ampicillin in LB media? - (reply: 6)
  203. SOB media - (reply: 4)
  204. Culture media for Nitrifying Organisms - (reply: 1)
  205. Protein extraction from cell culture media - (reply: 9)