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Medium Related Discussions
  1. black spots are found in cells and medium - (reply: 1)
  2. problem in preparing minimal media containing chromium - (reply: 1)
  3. replacing fetal bovine serum with other types of bovine serum in culture medium - (reply: 1)
  4. How to adjust the pH of a bacterial culture medium using a buffer? - (reply: 3)
  5. Addition of 1% carbon sources into broth media - (reply: 1)
  6. Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
  7. Help! Antibiotic and Serum Concentration in Cell Culture Media - (reply: 1)
  8. CHO-K1 media - (reply: 1)
  9. media salinity and ph - (reply: 1)
  10. Adding glucose to growth media - (reply: 5)
  11. Proper source of ampicillin for e.coli growth medium? - (reply: 2)
  12. Is any other method to maintain without changing media, or store cell culture fo - (reply: 2)
  13. Creating a protocol for testing microbiology media - (reply: 2)
  14. Is there much use for animal-free microbiology tissue culture media? - (reply: 1)
  15. Incubation period for culture media - (reply: 1)
  16. Can I store media containing TPA (PMA) at 4 degrees? - (reply: 1)
  17. Media turning acidic quickly - (reply: 4)
  18. unpredictable results in growing w.coli in different media - (reply: 2)
  19. FACS with culture media - (reply: 2)
  20. Medium - (reply: 2)
  21. Media formulation with Insulin - (reply: 4)
  22. Interference of selective medium - (reply: 1)
  23. Expired medium - (reply: 3)
  24. Is hygromycin-containing medium light sensitive? - (reply: 1)
  25. RPMI 1640 media - (reply: 2)
  26. Transfection media change - (reply: 1)
  27. DMEM medium with HEPES and NaHCO3 - (reply: 1)
  28. Should imaging media formulation match growth media? - (reply: 1)
  29. After centrifuge in serum-free media, forming cell sheet, instead of pellet in t - (reply: 2)
  30. Question about growth media for A20 Cell Line - (reply: 1)
  31. Left media in water bath overnight - (reply: 1)
  32. TANNIC ACID CONTAINING MEDIA - (reply: 1)
  33. expired medium - (reply: 5)
  34. Tips on media preparation and how to dissolve starch? - (reply: 13)
  35. which media for isolation of microorganisms from the environment? - (reply: 6)
  36. LB media contamination - (reply: 3)
  37. Opti-MEM I Reduced Serum Medium - (reply: 1)
  38. double crosslinking in PBS or medium? - (reply: 2)
  39. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  40. Salt in medium - (reply: 4)
  41. Assay for measuring Salt in a medium - (reply: 2)
  42. Too much LB in medium - (reply: 12)
  43. How can i seed 2000 cell /well? (Using 200 microliter media and 96 plate, and as - (reply: 1)
  44. FCS in Serum free medium for better Cell Adhesion? - (reply: 1)
  45. M9 Media Broth turning green - (reply: 18)
  46. Making agar containing media - (reply: 7)
  47. Cell culture medium contamination? - (reply: 1)
  48. Using the wrong media - (reply: 2)
  49. conditioned media - (reply: 1)
  50. M63 minimal media problems - (reply: 3)
  51. selection of the media for ion exchange chromatography - (reply: 1)
  52. Can serum free medium inactivate trypsin? - (reply: 1)
  53. HBE cell culture CRL-2079 MEDIA question - (reply: 1)
  54. How Do You Determine What The Electron Acceptor/Donor In Your Media Is? - (reply: 4)
  55. When cell media becomes "used up", it acidifies - (reply: 3)
  56. tissue collection and transport medium - (reply: 3)
  57. MTT in just PBS or PBS and medium? - (reply: 3)
  58. Explanation of components in defined iPS medium - (reply: 2)
  59. death of transfected cells after first passage in G418 medium - (reply: 1)
  60. Growth media - OD at induction - growth phases... - (reply: 2)
  61. The use of supplemented Minimal Salts media to grow pseudomonas aeruginosa in 96 - (reply: 1)
  62. change media everyday (or every other day) for MTT assay? - (reply: 4)
  63. Immunofluorescence photobleaching, is mounting medium to blame? - (reply: 2)
  64. E. coli (ATCC 25922) growth in Minimal Medium - (reply: 5)
  65. Medium left at room temperature overnight - (reply: 5)
  66. Grow multiple Ecoli colonies in a single liquid media tube - (reply: 4)
  67. Bacterial Media Formulation - (reply: 2)
  68. Preparation of Rich Medium for cultivation of Zymomonas sp - (reply: 3)
  69. Addition of antibiotic to the culture medium - (reply: 1)
  70. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
  71. Pelleting cells by gentle centrifugation for addition to media - (reply: 13)
  72. Media doesn't change colour? - (reply: 4)
  73. Stromal conditioned medium (SCM) - (reply: 1)
  74. Pore formation in cells after adding amphotericin B in culture medium - (reply: 2)
  75. Injection of human cell culture conditioned media into nude mice - (reply: 1)
  76. Can I make an SDS stock in media and store? - (reply: 3)
  77. Quick question about making culture media. - (reply: 5)
  78. Effect of low and high nutrient medium on bacterial attachment? - (reply: 1)
  79. conditioned medium for ischaemia study - (reply: 1)
  80. different type of media for cell culture - (reply: 1)
  81. Can medium containing starch be autoclaved? - (reply: 36)
  82. Preparing a conditioned medium from cancer cells - (reply: 3)
  83. Adjusting pH of RPMI/ bicarbonate containing media - (reply: 9)
  84. Help with media filtration - (reply: 5)
  85. Lentiviral media - HEPES buffered DMEM - do I titrate? - (reply: 1)
  86. Purpose Of Fatty Acids In Media - (reply: 1)
  87. Help w/ Media Preparation - (reply: 3)
  88. How long Kanamycin can last in broth medium? - (reply: 3)
  89. Taking Culture Medium Samples for LDH Assay - (reply: 2)
  90. Increasing cell growth rate in plain M9 medium - (reply: 1)
  91. culture media for pancreatic cancer stem cells - (reply: 2)
  92. Use-by-date of antibiotic & serum in media - (reply: 6)
  93. E. coli growing on plates and bot not in LB medium - (reply: 10)
  94. Looking for Staib agar dehydrated medium - (reply: 4)
  95. precipitation of copper in minimal medium - (reply: 5)
  96. What will happen if the cell culture medium is suddenly changed to another one? - (reply: 5)
  97. Help, I accidently warmed my media componemts to 57C! - (reply: 3)
  98. Reagent used to adjust pH of cell culture media - (reply: 3)
  99. Powder indomethacin in cell culture media?? - (reply: 2)
  100. Can i still use freezing medium if i used PES filter to filter my DMSO? - (reply: 1)
  101. Precipitating growth medium - (reply: 2)
  102. Not sure how to make HEPES or MOPS buffered media - (reply: 6)
  103. Pichia growth in MGY/MGYH media - (reply: 1)
  104. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
  105. Left my media at room temperature for 48 hours; okay to use? - (reply: 4)
  106. MEDIA REDOX STATUS - (reply: 3)
  107. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
  108. media supplements - (reply: 2)
  109. Culture media for BGMK-hDAF - (reply: 3)
  110. Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
  111. Elisa using HEK culture medium - (reply: 14)
  112. changing cell culture media - (reply: 1)
  113. Determining whether media contains serum or not? - (reply: 5)
  114. Retrovirus production & media color (pH) - (reply: 3)
  115. Calculating the amount of the desired cells in the medium ? - (reply: 3)
  116. How long do growth factors last in complete media? - (reply: 3)
  117. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
  118. yeast growth in SC-ura medium - (reply: 1)
  119. miRNA extraction from cell-conditioned media? - (reply: 1)
  120. Pseudomonas aeruginosa growth very slow in N-minimal media - (reply: 2)
  121. Microalgae media - pH adjustment - (reply: 1)
  122. cells shrink, detach from each other when changing media - (reply: 3)
  123. Preparation of Conditioned Medium - (reply: 1)
  124. cell culture media refrigeration - (reply: 4)
  125. ph? when i should corect the ph of media? - (reply: 6)
  126. Extrct RNA from fungus in broth medium - (reply: 2)
  127. Media formulations for MDA-MB-231, BT-474 cells and Caco-2 cells - (reply: 1)
  128. 5-FOA media not letting ANY cells grow... help! - (reply: 1)
  129. SUCROSE AS FRACTIONATION MEDIUM - (reply: 1)
  130. Adjusting pH of cell culture medium for ELISA - (reply: 1)
  131. testing media sterility - (reply: 2)
  132. Using a different media than recommended - (reply: 1)
  133. Medium formulation - (reply: 2)
  134. problems on the media for oryza sativa(rice) - (reply: 5)
  135. Freezing culture media - (reply: 4)
  136. antibiotics in media - (reply: 2)
  137. Preparing media with different pHs - (reply: 1)
  138. RPMI 1640 media - (reply: 2)
  139. Growing hybridoma cells in medium without serum - (reply: 2)
  140. Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
  141. Minimum salt medium preparation - (reply: 1)
  142. Expired media - How long after the expiration data is cell culture media good? (reply: 2)
  143. Live cell Imaging medium - (reply: 1)
  144. Concentrated conditioned medium for western blot - (reply: 1)
  145. Filter Sterilization of Hot Gellan Gum Media - (reply: 3)
  146. problems with MTT assay (some formazan crystals in the medium) - (reply: 1)
  147. E.coli cells lyse in minimal media - (reply: 3)
  148. cell suspencion and medium - (reply: 5)
  149. Cloudy/Turbid media after subculture - (reply: 3)
  150. Autoclave before adding MgCl2 and MgSO4? - SOB medium receipt (reply: 4)
  151. Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
  152. siRNA transfection - 7-day study - serum free media? (reply: 2)
  153. MTT assay medium with phenol red - (reply: 2)
  154. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
  155. cell culture media - glutamine! - (reply: 2)
  156. M9 medium??? - (reply: 1)
  157. Media - a beautiful pink! - NaOH in media (reply: 3)
  158. IF coverslipping slides / mounting medium / sealer - (reply: 2)
  159. Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
  160. Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
  161. Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
  162. Insulin supplements to cell culture media - (reply: 1)
  163. growth of E.coli in minimal media(m9) is very less - growth of E.coli in minimal media(m9) is very less (reply: 2)
  164. Running a western with cell media in gels? - (reply: 11)
  165. Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
  166. Yeast Minimal GS Medium Recipe? - (reply: 6)
  167. Preparing M&S medium - (reply: 4)
  168. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
  169. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
  170. Anitbiotic shelf life in liquid media at RT - (reply: 2)
  171. problems with 293T cells after medium removal!!! - (reply: 6)
  172. Enough media? - (reply: 1)
  173. recombinant ecoli in log phase overnight media suggestion - (reply: 2)
  174. cell culture media - (reply: 3)
  175. Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
  176. question - glucose additin to media (reply: 2)
  177. problem in M9 media - m9 media (reply: 4)
  178. Need pH 6 Cell culture media - (reply: 1)
  179. floating THP-1 cells - medium change advice - (reply: 2)
  180. Culture Media for stem cells - (reply: 1)
  181. Can buffering strength of media reduce with time ? - (reply: 4)
  182. Help!my 293T stop growth and become slim 24h after 1:3 splitting - unless you change new medium for them 24h after splitting (reply: 3)
  183. Keeping media at -80C - (reply: 3)
  184. Why does my media turn alkaline not acidic? - MCF-7 in EMEM (reply: 4)
  185. Concerning Cultivation of Monoclonal Antibodies - How long before antibodies spoil in media? (reply: 1)
  186. I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
  187. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
  188. Change of colour in cell culture media...? - (reply: 2)
  189. Adding L-glutamine to DMEM media w/o L-glutamine - (reply: 5)
  190. Bacteria Media (NZY+ broth) - (reply: 1)
  191. Mounting Medium for DIG in situ - (reply: 1)
  192. Bacteria growing on plate but not liquid media - (reply: 8)
  193. Trypsin/EDTA inactivation - which compound in the medium is responsible for inactivation? (reply: 5)
  194. what type of media for gingiva fibroblast tissue culture? - (reply: 3)
  195. too much nahco3 in medium - (reply: 1)
  196. HOW LONG CAN I STORE MY MEDIA...? - (reply: 3)
  197. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
  198. Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
  199. Can I IP from media? - to use for SDS-PAGE? (reply: 1)
  200. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
  201. satbility of DMEM media - (reply: 1)
  202. Why does medium turn yellow fast during the intensive synthesis of exogenous pro - Is it the by-product of metabolism?Is it lactic acid? (reply: 2)
  203. Half life o acetaminophen - on culture media (reply: 1)
  204. Sodium pyruvate in medium - (reply: 1)
  205. precipitation of secreted protein from growth media - (reply: 1)
  206. What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
  207. Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
  208. Cellular proteins in medium - (reply: 1)
  209. how long to autoclave the medium - (reply: 2)
  210. western blot using culture media - (reply: 4)
  211. Storage of Freezing Media - (reply: 2)
  212. Hoe much media needed for transfection complex? - transfection (reply: 9)
  213. low serum media and FCS - (reply: 2)
  214. Cell culture media - require information on media (reply: 3)
  215. Freezing cells- Media or FBS ? - (reply: 2)
  216. phenol red in media - (reply: 5)
  217. LB and SB electrophoresis media - (reply: 1)
  218. Daily medium change - (reply: 3)
  219. Calcium Phosphate transfection help - cells detaching after media change (reply: 1)
  220. My cells died after changing media... - (reply: 6)
  221. culture medium sterilization - (reply: 3)
  222. NSC-34 Cells sloughing off at media change - (reply: 4)
  223. which medium to use in cocultures - (reply: 3)
  224. Critical column of media above cells for access of oxygen - (reply: 1)
  225. question about media color - (reply: 4)
  226. adding bicarbonate to medium...help please! - (reply: 1)
  227. transformation medium - (reply: 2)
  228. How much cell medium for 1 week of culturing? - (reply: 1)
  229. adding fungicidies to the media in plant tissue culture - (reply: 2)
  230. Basal Media preparation - (reply: 2)
  231. iPS media - (reply: 1)
  232. Conditioned media preparation - (reply: 3)
  233. Subculture in low glucose media - (reply: 2)
  234. Cells suddenly died after changing the medium... why? - (reply: 4)
  235. DMEM vs RPMI for primary mouse lymphocytes - Which media for primary mouse lymphocyte? (reply: 2)
  236. New Gibco Media bottles - (reply: 1)
  237. Alternative media for PC-12 cells - (reply: 2)
  238. cho cell line adoptation to serum free medium - (reply: 1)
  239. antibiotic stability in culture medium - (reply: 1)
  240. Serum free culture media 293T cells? - (reply: 1)
  241. How long do you warm up media? - (reply: 9)
  242. Protein purification from Insect media - (reply: 1)
  243. conditioned medium - how to prepare conditioned media (reply: 2)
  244. Cell culture - Culture medium (reply: 1)
  245. Media for squamous cells - (reply: 3)
  246. Oxygen diffusion through cell culture medium - (reply: 2)
  247. Conditioned media question - (reply: 2)
  248. huvec change medium?? - (reply: 5)
  249. opti-mem as a serum starvation media - (reply: 1)
  250. 2,4-D substitute for Gamborg's B5 medium??? - (reply: 2)
  251. 5% vs 10% CO2 (with their corresponding media) - How relevant it is? (reply: 5)
  252. Transfection problem - milky media - (reply: 1)
  253. aliquot media recude contamination? - (reply: 3)
  254. Isolation of secreted proteins from culture media - (reply: 2)
  255. Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
  256. Media prep question - (reply: 6)
  257. vogel mineral growth media for fungi - (reply: 1)
  258. Storing conditioned media - (reply: 3)
  259. Cytotoxicity of hydrophobic substances - How to dissolve highly hydrophobic substances in aqueus growth medium? (reply: 2)
  260. Semisolid Medium For HL 60 cells - (reply: 4)
  261. Problem Maintaining pH in Culture Media - (reply: 4)
  262. NPB medium - (reply: 3)
  263. L.Lactis culture - M17 media (reply: 6)
  264. expression in minimal media - (reply: 14)
  265. media - (reply: 2)
  266. Treating cells with chemicals and how to dilute into medium - (reply: 5)
  267. Chromogenic Media - (reply: 1)
  268. Wnt 3a conditional medium - (reply: 1)
  269. Shelf life of ampicillin in LB media? - (reply: 6)
  270. SOB media - (reply: 4)
  271. Culture media for Nitrifying Organisms - (reply: 1)
  272. Protein extraction from cell culture media - (reply: 9)