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Medium Related Discussions
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Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
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Elisa using HEK culture medium - (reply: 14)
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changing cell culture media - (reply: 1)
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Determining whether media contains serum or not? - (reply: 5)
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Retrovirus production & media color (pH) - (reply: 3)
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Calculating the amount of the desired cells in the medium ? - (reply: 3)
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How long do growth factors last in complete media? - (reply: 3)
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What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
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yeast growth in SC-ura medium - (reply: 1)
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miRNA extraction from cell-conditioned media? - (reply: 1)
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Pseudomonas aeruginosa growth very slow in N-minimal media - (reply: 2)
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Microalgae media - pH adjustment - (reply: 1)
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cells shrink, detach from each other when changing media - (reply: 3)
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Preparation of Conditioned Medium - (reply: 1)
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cell culture media refrigeration - (reply: 4)
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ph? when i should corect the ph of media? - (reply: 6)
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Extrct RNA from fungus in broth medium - (reply: 2)
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5-FOA media not letting ANY cells grow... help! - (reply: 1)
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SUCROSE AS FRACTIONATION MEDIUM - (reply: 1)
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Adjusting pH of cell culture medium for ELISA - (reply: 1)
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testing media sterility - (reply: 2)
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Using a different media than recommended - (reply: 1)
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Medium formulation - (reply: 2)
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problems on the media for oryza sativa(rice) - (reply: 2)
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Freezing culture media - (reply: 4)
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antibiotics in media - (reply: 2)
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Preparing media with different pHs - (reply: 1)
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RPMI 1640 media - (reply: 2)
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Growing hybridoma cells in medium without serum - (reply: 2)
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Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
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Minimum salt medium preparation - (reply: 1)
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Expired media - How long after the expiration data is cell culture media good? (reply: 2)
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Live cell Imaging medium - (reply: 1)
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Concentrated conditioned medium for western blot - (reply: 1)
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Filter Sterilization of Hot Gellan Gum Media - (reply: 3)
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E.coli cells lyse in minimal media - (reply: 3)
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cell suspencion and medium - (reply: 5)
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Cloudy/Turbid media after subculture - (reply: 3)
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Autoclave before adding MgCl2 and MgSO4? - SOB medium receipt (reply: 4)
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Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
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siRNA transfection - 7-day study - serum free media? (reply: 2)
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MTT assay medium with phenol red - (reply: 2)
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Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
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cell culture media - glutamine! - (reply: 2)
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M9 medium??? - (reply: 1)
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Media - a beautiful pink! - NaOH in media (reply: 3)
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IF coverslipping slides / mounting medium / sealer - (reply: 2)
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Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
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Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
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Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
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Insulin supplements to cell culture media - (reply: 1)
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growth of E.coli in minimal media(m9) is very less - growth of E.coli in minimal media(m9) is very less (reply: 2)
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Running a western with cell media in gels? - (reply: 11)
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Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
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Yeast Minimal GS Medium Recipe? - (reply: 6)
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Preparing M&S medium - (reply: 4)
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solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
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A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
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Anitbiotic shelf life in liquid media at RT - (reply: 2)
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problems with 293T cells after medium removal!!! - (reply: 2)
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Enough media? - (reply: 1)
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recombinant ecoli in log phase overnight media suggestion - (reply: 2)
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cell culture media - (reply: 3)
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Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
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question - glucose additin to media (reply: 2)
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problem in M9 media - m9 media (reply: 4)
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Need pH 6 Cell culture media - (reply: 1)
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floating THP-1 cells - medium change advice - (reply: 2)
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Culture Media for stem cells - (reply: 1)
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Can buffering strength of media reduce with time ? - (reply: 4)
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Help!my 293T stop growth and become slim 24h after 1:3 splitting - unless you change new medium for them 24h after splitting (reply: 3)
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Keeping media at -80C - (reply: 3)
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Why does my media turn alkaline not acidic? - MCF-7 in EMEM (reply: 4)
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Concerning Cultivation of Monoclonal Antibodies - How long before antibodies spoil in media? (reply: 1)
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I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
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Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
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Change of colour in cell culture media...? - (reply: 2)
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Adding L-glutamine to DMEM media w/o L-glutamine - (reply: 5)
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Bacteria Media (NZY+ broth) - (reply: 1)
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Mounting Medium for DIG in situ - (reply: 1)
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Bacteria growing on plate but not liquid media - (reply: 8)
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Trypsin/EDTA inactivation - which compound in the medium is responsible for inactivation? (reply: 5)
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what type of media for gingiva fibroblast tissue culture? - (reply: 3)
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too much nahco3 in medium - (reply: 1)
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HOW LONG CAN I STORE MY MEDIA...? - (reply: 3)
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Cell culture medium instead of Nucleofector solution?? - (reply: 3)
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Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
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Can I IP from media? - to use for SDS-PAGE? (reply: 1)
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Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
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satbility of DMEM media - (reply: 1)
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Why does medium turn yellow fast during the intensive synthesis of exogenous pro - Is it the by-product of metabolism?Is it lactic acid? (reply: 2)
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Half life o acetaminophen - on culture media (reply: 1)
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Sodium pyruvate in medium - (reply: 1)
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precipitation of secreted protein from growth media - (reply: 1)
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What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
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Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
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Cellular proteins in medium - (reply: 1)
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how long to autoclave the medium - (reply: 2)
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western blot using culture media - (reply: 4)
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Storage of Freezing Media - (reply: 2)
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Hoe much media needed for transfection complex? - transfection (reply: 9)
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low serum media and FCS - (reply: 2)
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Cell culture media - require information on media (reply: 3)
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Freezing cells- Media or FBS ? - (reply: 2)
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phenol red in media - (reply: 5)
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LB and SB electrophoresis media - (reply: 1)
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Daily medium change - (reply: 3)
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Calcium Phosphate transfection help - cells detaching after media change (reply: 1)
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My cells died after changing media... - (reply: 6)
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culture medium sterilization - (reply: 3)
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NSC-34 Cells sloughing off at media change - (reply: 4)
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which medium to use in cocultures - (reply: 3)
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Critical column of media above cells for access of oxygen - (reply: 1)
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question about media color - (reply: 4)
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adding bicarbonate to medium...help please! - (reply: 1)
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transformation medium - (reply: 2)
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How much cell medium for 1 week of culturing? - (reply: 1)
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adding fungicidies to the media in plant tissue culture - (reply: 2)
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Basal Media preparation - (reply: 2)
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iPS media - (reply: 1)
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Conditioned media preparation - (reply: 3)
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Subculture in low glucose media - (reply: 2)
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Cells suddenly died after changing the medium... why? - (reply: 4)
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DMEM vs RPMI for primary mouse lymphocytes - Which media for primary mouse lymphocyte? (reply: 2)
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New Gibco Media bottles - (reply: 1)
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Alternative media for PC-12 cells - (reply: 2)
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cho cell line adoptation to serum free medium - (reply: 1)
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antibiotic stability in culture medium - (reply: 1)
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Serum free culture media 293T cells? - (reply: 1)
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How long do you warm up media? - (reply: 9)
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Protein purification from Insect media - (reply: 1)
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conditioned medium - how to prepare conditioned media (reply: 2)
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Cell culture - Culture medium (reply: 1)
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Media for squamous cells - (reply: 3)
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Oxygen diffusion through cell culture medium - (reply: 2)
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Conditioned media question - (reply: 2)
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huvec change medium?? - (reply: 4)
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opti-mem as a serum starvation media - (reply: 1)
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2,4-D substitute for Gamborg's B5 medium??? - (reply: 2)
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5% vs 10% CO2 (with their corresponding media) - How relevant it is? (reply: 3)
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Transfection problem - milky media - (reply: 1)
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aliquot media recude contamination? - (reply: 3)
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Isolation of secreted proteins from culture media - (reply: 2)
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Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
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Media prep question - (reply: 6)
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vogel mineral growth media for fungi - (reply: 1)
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Storing conditioned media - (reply: 3)
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Cytotoxicity of hydrophobic substances - How to dissolve highly hydrophobic substances in aqueus growth medium? (reply: 2)
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Semisolid Medium For HL 60 cells - (reply: 4)
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Problem Maintaining pH in Culture Media - (reply: 4)
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NPB medium - (reply: 3)
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L.Lactis culture - M17 media (reply: 6)
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expression in minimal media - (reply: 14)
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media - (reply: 2)
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Treating cells with chemicals and how to dilute into medium - (reply: 5)
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Chromogenic Media - (reply: 1)
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Wnt 3a conditional medium - (reply: 1)
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Shelf life of ampicillin in LB media? - (reply: 6)
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SOB media - (reply: 4)
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Culture media for Nitrifying Organisms - (reply: 1)
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Protein extraction from cell culture media - (reply: 9)