vogel mineral growth media for fungi - (May/13/2009 )

Hi
Would like to know to grow the P.chrysosporium on vogel mineral medium ,may i know what are the best composition of trace elements.Where can i obtain more information on this?

TQ

lovey5dovey on May 14 2009, 04:09 AM said:

Vogel's 50X salts (Microbial Genetics Bulletin 13:42-43, 1956). In 750 ml. distilled water, dissolve successively with stirring at room temperature:


Na3 citrate, 5 1/2 H20 150 grams(NOTE, you may substitute 125 g Na3 Citrate 2 H2O)
KH2PO4, anhydrous 250 grams
NH4NO3, anhydrous 100 grams
MgSO4, 7 H20 10 grams
CaCl2, 2 H20 5 grams
Trace Element Solution (see below) 5 ml.
Biotin Solution (see below) 2.5 ml.

The resulting total volume is about 1.00 liter. Chloroform (2 ml. ) is added as a preservative.
Store at room temperature.

Vogels Medium N (Minimal) is

20 mL Vogel's Salts
15 grams Sucrose
1 liter dH2O

add 15 grams agar for solid medium

The trace element solution (containing citric acid as a solubilizing agent) is made up as follows: In 95 ml. distilled water, dissolve successively with stirring at room temperature:

Citric acid, 1 H20 5. 00 grams
ZnSO4, 7 H20 5. 00 grams
Fe(NH4)2(SO4)2, 6 H20 1. 00 gram
CuSO4, 5 H20 0. 25 gram
MnSO4, 1 H20 0. 05 gram
H3BO3, anhydrous 0. 05 gram
Na2MoO4, 2 H20 0. 05 gram

The resulting total volume is about 100 ml. Chloroform (1 ml. ) is added as a preservative, and the trace element solution is stored at room temperature.

The biotin solution is prepared by dissolving 5.0 mg. biotin in 50 ml. distilled water. The solution obtained is dispensed in test tubes and stored in the frozen state.


This is an old medium. Today, I would filter-sterilize the x50 stock (not use chloroform). I think it best to prepare the x1 liquid medium by asceptically adding the stock to sterile water. If you need agar, sterilize this in the water, cool to about 56 oC and then add the stock.

Hope this helps.