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problems on the media for oryza sativa(rice) - (Jul/28/2011 )

hi all,
i wanna ask a Q on the problems happened to my CIM (callus induction media)

after about a 2-3weeks i cultured the seeds of Oryza sativa, i already got the calli and i subcultured the calli to new media.

but then, after about 1week, the calli turned yellow/brown color as well as the media.

why actually this happen? i'm worried already

is it the technique used or the problem with the media??

anyone,please help.. tq

-girl in pink-

This generally happens when there are phenolics in the media, You have to search for the cause which is causing production of these compounds and subsequent death of calli. most probabaly the problem could be with the handling of calli.

-tellmeplz-

i did cell culture back in university (we're talking first year undergrad, so over 10 years now :blink:) but if i remember right, that happened when the tissue was oxidised, so look for some antioxidants to add to your media after callus has been formed.

another common problem was crystallisation of the tissue, kind of what happens to the politician in the x-men movie...

-toejam-

In our lab we used PhytoTechLab A126 - antioxidant mixture to prevent oxidation, however, it works different for each plant species.  Another thing is you have to be careful not to over trim the tissues in which phenolic exudates most often when plant tissue become damaged. 

 

However, I have a question in regard to producing rice shoots.  Do you have any experience with rice shoots in culture? What type of cytokinins/auxins you've used?

-shinome-

In our lab we used PhytoTechLab A126 - antioxidant mixture to prevent oxidation, however, it works different for each plant species.  Another thing is you have to be careful not to over trim the tissues in which phenolic exudates most often when plant tissue become damaged. 

 

However, I have a question in regard to producing rice shoots.  Do you have any experience with rice shoots in culture? What type of cytokinins/auxins you've used? 

you might enhance your shoot induction medium with Half strength MS salt, myo ino, BA, Zeatin, IAA, Casein Hydrolisat, Proline, Sucrose and gelrite. I use medium and it work for my routine lab work. for further each concentration, you can PM me. Good Luck.

 

 

 

 

 

-tamarine-

hi all,
i wanna ask a Q on the problems happened to my CIM (callus induction media)

after about a 2-3weeks i cultured the seeds of Oryza sativa, i already got the calli and i subcultured the calli to new media.

but then, after about 1week, the calli turned yellow/brown color as well as the media.

why actually this happen? i'm worried already

is it the technique used or the problem with the media??

anyone,please help.. tq

what are you medium composed of? 

try to re-check your attitude on how you handle the calli.  the wound also cause browning. to minimize it, transfer directly to the medium. 

-tamarine-