Too much LB in medium - (Feb/26/2014 )

Hallo,

I have a quick question: I used the double amount of LB (the flask said 10g/l) I accidently used 20g/l.

Now I wonder: does this make a difference? I would think not, its just more food for the bacteria. But what about antibiotics you use? Would this perhaps be a problem or should it not be a big problem?

Altough, I can imagine that because of the more food present the bacteria that have not been transformed like they should have been, perhaps are able to grow due to the excess in food?

I am also assuming that the salt concentration , although being doubled does not really play a huge role.

Why not simply dilute it by a factor of two.

Too late, already poured the plates.

Salt can play a huge role in this - the osmotic strength of the solution is very important for bacterial growth.  Too much and the bacteria will dehydrate and not grow...

Best to throw out and make some fresh plates.

I agree with Bob1.

Be rigorous in your science.  

Phil Geis on Thu Feb 27 03:26:16 2014 said:

Be rigorous in your science.  

This...this is good advice always!

Aside form being too salty to grow, some antibiotics are salt sensitive and will not work in high salt environment (ie, Kanamycin, blasticidin and hygromycin).

Interesting!

Where do you get this knowledge ?

Is it mentioned in the papers you get with the antibiotics?

perneseblue on Fri Feb 28 04:13:18 2014 said:

Aside form being too salty to grow, some antibiotics are salt sensitive and will not work in high salt environment (ie, Kanamycin, blasticidin and hygromycin).

You can find such interesting information and more in, molecular Cloning: A Laboratory Manual, Maniatis.