Sodium pyruvate in medium - (Apr/28/2010 )
Hi everyone,
I'm new to cell culture and would like to know the proliferative effects of H2O2 on endothelial cells. Our lab use HUVEC-C from atcc but the medium required for this cell line is F12K, which contains sodium pyruvate.
I tried to add H2O2 and test its effect on endothelial cells but there's no change in the cell proliferation and i think it's because of the sodium pyruvate present in the medium. Does anyone know that if sodium pyruvate is a necessary nutrient for this cell and if not, can i remove it from the medium and how as i cannot find out any F12K (without sodium pyruvate) can be purchased commercially...
Please help me......
-sndrlai-
sndrlai on Apr 28 2010, 08:31 AM said:
Hi everyone,
I'm new to cell culture and would like to know the proliferative effects of H2O2 on endothelial cells. Our lab use HUVEC-C from atcc but the medium required for this cell line is F12K, which contains sodium pyruvate.
I tried to add H2O2 and test its effect on endothelial cells but there's no change in the cell proliferation and i think it's because of the sodium pyruvate present in the medium. Does anyone know that if sodium pyruvate is a necessary nutrient for this cell and if not, can i remove it from the medium and how as i cannot find out any F12K (without sodium pyruvate) can be purchased commercially...
Please help me......
I'm new to cell culture and would like to know the proliferative effects of H2O2 on endothelial cells. Our lab use HUVEC-C from atcc but the medium required for this cell line is F12K, which contains sodium pyruvate.
I tried to add H2O2 and test its effect on endothelial cells but there's no change in the cell proliferation and i think it's because of the sodium pyruvate present in the medium. Does anyone know that if sodium pyruvate is a necessary nutrient for this cell and if not, can i remove it from the medium and how as i cannot find out any F12K (without sodium pyruvate) can be purchased commercially...
Please help me......
I have a similar question about sodium pyruvate listed under cell biology (it probably should be listed under tissue and cell culture). Check out my response. I'll let you know if i get anything.
-RAinsmallpharma-