Protocol Online logo
Top : New Forum Archives (2009-): : Protein and Proteomics

Concentrated conditioned medium for western blot - (May/22/2011 )

I ran concentrated conditioned medium on western blot, and I got a huge white colour bands around 60kDa, I think these bands must be from albumin, and my gel ran funny. The conditioned medium containing 10% FCS. May I know how can I get rid of the albumin in the conditioned media after I concentrated them? I want to look at BDNF protein level in the conditioned medium. Thanks!

-skyyingkai-

there are a number of albumin removal kits available.

see this google query.

-mdfenko-