E. coli (ATCC 25922) growth in Minimal Medium - (Jun/26/2013 )

Hi everyone,

I am trying to grow E.coli (ATCC 25922) in minimal medium of following composition (per liter): Na2HPO4 (68.16), K2HPO4 (3.83), NH4Cl (1.07), NaCl (0.50 g), CaCl2.2H2O (0.0147 g), MgSO4 (0.1 g) and glucose (4 g).

I tried to grow overnight culture in above minimal medium but bacteria showing no growth (OD₆₀₀ does not show any growth).

Can somebody help me with this that why I am not getting any growth. Thanks

Check the pH of the medium. It should be around 7-7.5.
You could also try adding some of the common supplements, such as thiamine.
This is a wild type strain, and should grow in that medium.
Are you sure the cells are viable?

Thanks for your reply.

The pH of the medium is around 7.4. I have not tried thiamine. Because I thought that it would grow without it.

How much thiamine should be used for optimum growth of bacteria and what is it function?

Where do your cells come from? How do you know they are viable?

phage434 on Thu Jun 27 13:40:33 2013 said:

I have taken them from Life and social science department. I have grown the cells in Nutrient Broth (NB) and they showed optimal growth. Overnight culture showing OD₆₀₀ ~ 1.2. I also did viable counts and measure the CFU in NB showing approx. 2.8 x 10⁸ cells/ml.

i assume you're running a parallel control in something like TSB.

I don't understand why folks are so reticent to look at the literature. The following reports a minimal medium supporting growth of the isolate you mentioned.
Antimicrob Chemother. 1992 Feb;29(2):121-7.
Maintenance requirements of Escherichia coli ATCC 25922 in the presence of sub-inhibitory concentrations of various antibiotics.