Protocol Online logo
Top : New Forum Archives (2009-): : Tissue and Cell Culture

How long do you warm up media? - (Sep/02/2009 )

Any damage could be done to media if I warm it up too long?

Say....like half of a day before use?

I just forgot that I put it in 37'C water bath in the morning..

It was a one of those crazy days that i was mentally off..

Then 6 o'clock in the evening, same day, I just found a bottle of media, and was like "Hmm...that one seems familiar.........uh?......argg! it is mine!!!"

Would it be okay after extra hours of warming?

If not, why??

thx. :P

-iamdhan-

if it is plain media I would go with it.

-Stephan-

I did forget one bottle over night at 37C. It was perfectly fine.

-madrius1-

Cool. <_< silly me, i worry too much.

-iamdhan-

iamdhan on Sep 3 2009, 03:54 AM said:

Any damage could be done to media if I warm it up too long?

Say....like half of a day before use?

I just forgot that I put it in 37'C water bath in the morning..

It was a one of those crazy days that i was mentally off..

Then 6 o'clock in the evening, same day, I just found a bottle of media, and was like "Hmm...that one seems familiar.........uh?......argg! it is mine!!!"

Would it be okay after extra hours of warming?

If not, why??

thx. ;)


what media do you mean and for what? And was whatever you wanted to put in the media allready in it?

when I grow cells I put the media at 37C the evening before I use it, and sometimes even 1 day before that.
The media wont go bad... I so no reason why to worry.

-pito-

Cell culture medium containing L-glutamine should only be warmed for short periods of time as the L-glut is unstable. Long periods in the waterbath will cause it to degrade.

-bob1-

i never warm up the media in a water bath. I just take it out in the morning and bring it up to room temperature. and I never had any problems with both established lines and primary cells.

-toprak-

toprak on Dec 9 2009, 06:43 AM said:

i never warm up the media in a water bath. I just take it out in the morning and bring it up to room temperature. and I never had any problems with both established lines and primary cells.


Ya. I would do the same if don't need the media immediately.
Otherwise I'll warm it in waterbath for 30 min if I am in hurry.

-sanjiun-

sanjiun on Dec 9 2009, 03:14 AM said:

Ya. I would do the same if don't need the media immediately.
Otherwise I'll warm it in waterbath for 30 min if I am in hurry.


I warm mine up in the waterbath for 30 min, too. I start the hood, put the medium in the waterbath, and have a cup of coffee. Then I'm ready to go. :) However, long periods of incubation at 37 C will also cause amphotercin to degrade as well as the L-glut.

-lab rat-

lab rat on Dec 9 2009, 08:38 AM said:

sanjiun on Dec 9 2009, 03:14 AM said:

Ya. I would do the same if don't need the media immediately.
Otherwise I'll warm it in waterbath for 30 min if I am in hurry.


I warm mine up in the waterbath for 30 min, too. I start the hood, put the medium in the waterbath, and have a cup of coffee. Then I'm ready to go. :P However, long periods of incubation at 37 C will also cause amphotercin to degrade as well as the L-glut.

Amphoterecin???? you don't want that in your culture, antibiotics and especially antimycotics are bad - they can really screw up your culture and mask low level infections which will cause spurious results.

-bob1-