DMEM vs RPMI for primary mouse lymphocytes - Which media for primary mouse lymphocyte? (Oct/21/2009 )

Hey guys,

Was wondering which media is good for proliferation assay for mouse splenocytes? T-hanks!

-S

either should be fine, you will need to add lots of serum and probably some growth factors.

Hello

My recipe for mature B cell media:

RPMI + 50um B-mercaptoethanol, 10% FCS, 10mM HEPES pH 7.4, 0.1mM non-essential amino acids, 2mM L-Glutamine, 1% sodium pyruvate

Hope this helps

Clare

smanivannan on Oct 21 2009, 11:23 PM said: