cloning Methods, Protocols and Troubleshootings

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Cloning Related Discussions

New to Topo cloning - (reply: 4)
cloning vector and restriction mapping - (reply: 2)
software to replace Vector NTI and a simple cloning problem - (reply: 3)
cloning into bacterial expression vector - problem in E.coli ttransformation (reply: 4)
cloning methods - something other than the usual (reply: 4)
problems with subcloning - (reply: 4)
Cloning hell - (reply: 1)
Cloning hell - (reply: 1)
Cloning Help! - (reply: 6)
EGFP cloning and membrane protein - (reply: 2)
gene cloning questions - (reply: 3)
Cloning bisulphite converted amplicon - Need help on getting more clones (reply: 4)
Blunt end ligation problem - (reply: 3)
directional cloning problem - two-digested vector and insert - ligate - no colonies?? (reply: 2)
Blunt end ligation problem - (reply: 2)
Cloning into pGBKT7 vector - (reply: 4)
promoter cloning - (reply: 1)
cloning past stop codon - 3'end stop codon cloning (reply: 1)
Making blunt ends DNA with T4 Polymerase - (reply: 6)
Working with TOPO II cloning vector - (reply: 2)
Unique cohesive overhangs cloning + cohesive/blunt ends cloning - molar ratio / use of PEG in cohesive-blunt ligation (reply: 8)
funny result from pGEX-insert cloned in DH5 alpha - insert is the wrong size! (reply: 2)
Easiest cloning strategy for deleting part of plasmid using adapters? - (reply: 1)
Book with tips and tricks for cloning - (reply: 3)
HOW to clone some kinases? - clone CDK2,CDK3,MAPK14 from HeLa (reply: 2)
problem in cloning, if any one can help me out - (reply: 2)
TOPO® TA cloning - (reply: 3)
Subcloning and Mutations - (reply: 1)
PCR cloning problem. Pls help...very urgent! - PCR cloning problem. Pls help...very urgent!!!!!!! (reply: 2)
blunt ligation of large fragment - (reply: 6)
Random Library Cloning - (reply: 1)
DNA cloning problem - (reply: 3)
Cloning of plasmid - (reply: 3)
Please comment on my cloning strategy - (reply: 5)
Large MSCV Retroviral Vector Cloning --- Help Needed! - (reply: 3)
Can i use same RE to check for insert? - blunt end cloning (reply: 8)
vector seq & Multiple cloning site design. - (reply: 2)
linearized DNA (entry clone) for LR recombination? - (reply: 1)
how can I clone a CYP450? - (reply: 1)
5 inserts, 1 colony..dreaming of a better cloning world - (reply: 6)
PCR cloning - (reply: 5)
How to conduct DNA cloning if plasmid's restriciton site is un-unique - (reply: 5)
Cloning of 3 kb fragment - (reply: 5)
lentiviral systems for overexpression: clone in full cDNA or ORF? - (reply: 3)
4 subunit gene: How to clone? - (reply: 1)
Ligation using TA cloning - (reply: 1)
Problem with promoter PCR and cloning - (reply: 3)
DNA blunting - DNA blunting troubleshoot (reply: 4)
TA Cloning - TA cloning (reply: 2)
Sub-cloning with XhoI and SalI digested frament - Problem subcloning with XhoI and SalI (reply: 8)
how to clone my insert DNA into vector? - (reply: 11)
Cloning: blunt and sticky - (reply: 10)
blunt end ligation - (reply: 2)
Blunting by Klenow - Excess dNTPs (reply: 2)
TA cloning and 3 fragment ligation problems - (reply: 1)
Multigene cloning - Looking for a cheap solution - (reply: 4)
Domain-Exchange via Blunt End Cloning of 2 PCR-Products - (reply: 4)
Basics of cloning - (reply: 1)
Subcloning problem - (reply: 9)
Lack of Expression in pQE30 clone - (reply: 8)
Cloning - False positive results - HELP!! (reply: 4)
Random DNA library problem - repair, ligation, digestion problem? TOPO and Zero Blunt (reply: 7)
Adding 3' A-overhangs for TA cloning - (reply: 4)
Wrong cloning product once sequenced - (reply: 3)
My TOPO-TA cloning did not work. - (reply: 10)
in-fusion cloning - (reply: 2)
incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
Cloning with methylated vector - (reply: 2)
How much total RNA is needed to isolate and clone small RNA? - (reply: 1)
How to change Blunt End to sticky ? - (reply: 1)
lux gene cloning using Sal I as restriction enzyme - (reply: 1)
cloning of a fluorecent PCR product - (reply: 1)
Cloning directionality and terminator availability - is lac z gene terminator is enough for my gene termination?? (reply: 1)
Non-directional cloning - (reply: 1)
Cloning from genomic DNA - (reply: 7)
Promoter cloning... - Problems (reply: 3)
Blunt end ligation - (reply: 8)
Zero Blunt cloning - ligation problem? (reply: 3)
Possible to add a linker to only one blunt end? - (reply: 4)
help on directional cloning - (reply: 3)
Problems during plasmid prep in cloning - (reply: 7)
Release of an insert from the cloning vector using restriction enzymes - (reply: 1)
pSUPERIOR cloning - please help! - (reply: 1)
cloning into the pBAD-GFPuv - (reply: 1)
commercial TA cloning vector with Luciferase - cloning promoter sequences (reply: 2)
Cloning 1.5kB gene into lentivirus--I'm stumped - (reply: 2)
Problems subcloning with the pL452 recombineering plasmid - (reply: 2)
Xba I subcloning problem - (reply: 6)
cloning kills - (reply: 5)
Why do I get only white colonies that do not grow in the midst liquid? - Troubes with cloning in pGEM-T (reply: 6)
TOPO TA Cloning - hundreds of colonies, 0 insert - (reply: 6)
M13 for cloning? - (reply: 4)
Better Molecular Biology Reference: Molecular Cloning vs. Short Protocols - (reply: 1)
cloning pGEMT - (reply: 4)
sequential PCR and purification before TA cloning - (reply: 1)
problem with cloning using pTYB2 - (reply: 2)
how to clone the promoter of a gene - (reply: 3)
genome amplification before cloning? - (reply: 2)
how to clone multiple inserts simulataneously - need to make a triple fusion (reply: 1)
cloning into plasmid (IMPACT kit from NEB) - (reply: 3)
cloning and transformation - (reply: 1)
ta cloning vectors - non commercial ta cloning vectors (reply: 1)
how to clone unstable DNA? - (reply: 4)
miRNA - cloning strategies - (reply: 2)
Techanical Question about cloning of gene - (reply: 2)
How to test if a cDNA clone from a plasmid is infectious? - (reply: 4)
Cloning of genes - (reply: 2)
DNA polymerase that produces blunt end and doesnt smear! - (reply: 2)
cloning of alternative splicing variants - (reply: 2)
Cloning in frame into pcDNA 3.1(+) vector - a quick question (reply: 6)
QUestion on subcloning a 80bp product from TOPO vector - (reply: 5)
Positional Cloning - (reply: 1)
TA cloning, sequencing results are vector self-ligation while bacterial PCR iden - (reply: 2)
RE cloning a 2.3kb fragment into a 4.7kb vector - (reply: 4)
Please help me analyze BSP cloned sequencing results. - (reply: 8)
How can I create a T vector for PCR product cloning - (reply: 2)
Help: PCR clone problem - (reply: 2)
Question on transcription start site and promoter cloning - (reply: 3)
Help in Clone Expression - Trasformants grow on plate but not in medium (reply: 2)
design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
Retroviral vector cloning help - (reply: 6)
cloning with pGEM T-easy vector - question about the vector. (reply: 2)
Sequencing:why do pcr AND cloning into plasmid? - (reply: 6)
CLONING LONG INSERT RATIO INS:VEC - (reply: 4)
Please comment on this simple cloning strategy - (reply: 6)
too many cloning false positives - (reply: 5)
How about vector sequence in cloning? - (reply: 9)
Cloning Problem ! - (reply: 6)
Cloned genes dont express! - (reply: 6)
Stable cell line - clone vs. population - (reply: 15)
Will TOPO TA cloning work on "old" purified PCR product? - (reply: 4)
Do I need to clone into gateway vector or pGEMT easy vector before cloning into - (reply: 1)
how to prepare c dna for cloning - (reply: 1)
RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
Cloning and Sequencing woes - (reply: 9)
cloning in pNL4-3 - (reply: 1)
Morphology of ES cell clones - Does this clone look like ES cells? (reply: 4)
Sal I cloning problems - (reply: 13)
cloning of 40 nucleotide repeat fragments - (reply: 4)
Ligation problem with PCR product cloning - (reply: 9)
problems in clone confirmation - (reply: 3)
large fragment cloning problem! Help needed - (reply: 2)
Cloning trouble - (reply: 7)
cloning - (reply: 2)
PCR product cloning - (reply: 6)
Stability and degradation of cloning reagents - May help resolve problematic cloning (reply: 1)
how to clone the genomic region for coding the mature miRNA - (reply: 2)
problem with bi-enzyme cloning - (reply: 4)
Calculating protein size - how to calculate the protein size when cloned in pet vector (reply: 1)
Blunt end cloning question - (reply: 1)
Molecular Cloning: A Laboratory Manual (3-Volume Set) - buy now or later? (reply: 4)
Double ligation - What about cloning two fragments in the same reaction? (reply: 5)
Blunt-end/cohesive ends cloning - (reply: 7)
cloning - (reply: 9)
gateway cloning - (reply: 1)
Bisulfite direct sequencing + cloning - (reply: 2)
cloning - (reply: 1)
Help with TOPO TA cloning – too long or too many insert - (reply: 1)
Cloning big inserts - (reply: 4)
cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector?? (reply: 2)
plasmid subcloning - (reply: 1)
Random Amplification + TA cloning = HATE - (reply: 2)
how important a terminator is in cloning? - (reply: 3)
Cloning 2kb+ gene by PCR - Is it doable? (reply: 3)
strange transformation products in subclone - (reply: 4)
BLUNT END LIGATION - DIFFICULTIES ON BLUNT END LIGATIONS (reply: 3)
sticky/blunt end cloning failure - (reply: 1)
cloning problem - (reply: 2)
Blunt end cloning - vector and insert preparation (reply: 9)
Blunt or sticky ligation-Which is more efficient? - (reply: 3)
E. coli stain for cloning that grow up very quick - Any one who could give the info??? (reply: 5)
cDNA cloning using Taq/pfu polymerase - (reply: 2)
lentiviral vector cloning problem - (reply: 1)
Using mRNA in cloning - (reply: 1)
compare pEGM T easy vector and TOPO TA cloning - (reply: 7)
Blunt ended ligation not working! - (reply: 3)
Problem in cloning - (reply: 8)
pros and cons of Blunt vs sticky ligation - (reply: 2)
Blunt end ligation problems, no ligation at all... - (reply: 2)
How can clone the complete miRNA primary transcript ? - (reply: 2)
primer design for subclone - (reply: 2)
Problems cloning a tiny insert into a huge vector - (reply: 1)
Problems with cloning HIV env gene - HIV cloning (reply: 4)
Blunt end vector........? - (reply: 2)
Cloning with BAC - (reply: 2)
cloning problem - (reply: 1)
My DNA fragment in agarose gel is in 4 degree - Can I extract the fragment and use it for cloning??? (reply: 6)
cloning, ligation, dephosphorylation - ligation, dephosphorylation (reply: 3)
unable to clone into pET29b and pET32b - sticky end ligation failing (reply: 2)
subcloning into pET21b failing - (reply: 7)
What exactly does it mean by cloning a toxic gene - (reply: 2)
cloning 708bp PCR prodyct into pET101 vector (TOPO) - (reply: 2)
shotgun cloning: gel shows inserts but only plasmid sequence obtained - (reply: 1)
Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
cloning a 3kb fragment into a 6kb vector - or maybe an EcoRI/HindIII double digest problem? (reply: 3)
route map for cloning a gene - steps involved in subcloning a gene (reply: 3)
Cloning toxic gene - (reply: 2)
Cloning PCR product blunt + restriction site - (reply: 2)
help in cloning 6kb pcr fragment into topo? - (reply: 5)
problem with cloning and sequencing. - (reply: 10)
orientation in cloning - (reply: 1)
Problems with TA cloning of bisulfite converted PCR product - (reply: 2)
Cloning into GATEWAY system - (reply: 3)
Need help with TOPO TA cloning problem! - TOPO TA clonig, ligation, no insert (reply: 23)
cloning in expression vector - (reply: 5)
Using single restriction enzyme SalI for cloning to a binary vector. How I will - Please its really very urgent...help me...help me.. (reply: 4)
klenow/T4 question - Making one end blunt (reply: 4)
Cloning of construct in SalI site - (reply: 3)
Need help about my subcloning pET15b. - (reply: 1)
primer design for PCR cloning - (reply: 1)
opposite sequence orientation in TA cloning - (reply: 2)
sticky and blunt DNA on gel migration - (reply: 5)
sticky end to blunt end? - cloning (reply: 3)
molecular cloning - TA cloning (reply: 3)
Gateway cloning with 3 entry vectors - (reply: 4)
Utter failure of directional cloning - need help - (reply: 5)
cloning faint bands - (reply: 5)
Is this clone the correct one? - Restriction digestion doubt (reply: 1)
Problem with TA cloning - (reply: 1)
Cloning using adaptors-->converting RE site - (reply: 4)
how to ensure that insert orientation is correct in TA cloning - (reply: 6)
Cloning problem: only frameshift mutants survive - (reply: 13)
BAC vecor cloning - (reply: 3)
AT-Rich Insert Cloning - (reply: 2)
How to buy a cDNA clone? - (reply: 2)
Why do you need your insert in the vector in both orientations? - Cloning (reply: 4)
blunt end - filling, polishing (reply: 1)
Creating short dsDNA from primers for cloning - (reply: 2)
cloning of 1 kb fragment in 14 kb vector - (reply: 3)
cloning problem ....... - (reply: 4)
Cloning small DNA fragments. Bacteria are not growing - (reply: 5)
cloning help about pBudCE4.1 - (reply: 1)
cloning of a big Plasmid - (reply: 3)
Loss of insert after cloning! - (reply: 3)
cloning problem - (reply: 6)
Blunt Cloning Large Insert/Smaller Vector - (reply: 9)
PCR cloning: neg self-ligation control, many colonies but no insert - (reply: 3)
Cloning Related Problem - Cloning in pBSK (reply: 1)
Problems with directional cloning using BsmBI and Xba I - (reply: 6)
Dephosphorylation for one cohesive, one blunt end ligation? - (reply: 1)
what's the difference between sequencing primer and cloning one. - confused (reply: 5)
Cloning trouble......help! - (reply: 4)
cloning problem of big inserts - (reply: 4)
how the multiple cloning site in cloning vector is create? - (reply: 4)
cloning problem - (reply: 3)
Cloning 50-100bp fragments. Need help. - i'm getting all blues... (reply: 10)
can blunt & sticky ends stick - (reply: 11)
help for no E coli. clone after transforming - (reply: 5)
Cloning a 60 bp pcr product and Mixing Pfx and Taq polymerases - (reply: 4)
PCR cloning problems - argh!!! (reply: 5)
Cloning/Miniprep Blues - High molecular weight bands iand no plasmid n my miniprep (reply: 3)
cloning shRNA into pLentilox 3.7 - (reply: 3)
site after blunting - (reply: 1)
Cloning w/pCR4 TOPO vector - problems obtaining clones (reply: 1)
blunt ending the vector followed by the ligation - (reply: 1)
Bisulfite sequencing - why cloning? - (reply: 3)
Inability to grow plasmid in culture - Molecular cloning (reply: 3)
Confused about cre-lox recombination system - Why is CreatorTM DNA cloning better over other cre-lox systems? (reply: 1)
Trouble cloning miRNAs - (reply: 5)
Problems with cloning - (reply: 4)
Issues with pcDNA3.1Directional cloning - (reply: 2)
Problem with cloning pGEMT easy vector system - (reply: 4)
cDNA cloning - (reply: 2)
polyA tail as 3' adaptor to clone miRNA - (reply: 1)
Amplify RANKL for cloning - (reply: 3)
Cloning with linkers? - (reply: 5)
Blunt end cloning PCR product - (reply: 6)
cloning impossible - (reply: 10)
cloning cDNA libraries - (reply: 4)
enzyme site after blunting - (reply: 7)
How to thansfer the single clone - (reply: 2)
Has any one successfully performed miRNA cloning? - miRNA cloning troubleshooting (reply: 5)
integration vector question - do i need to clone a full gene (reply: 1)
Quick ligation and blunting kits NEB - (reply: 7)
Uridines in linker design for miRNA cloning. - Why uridine is often used in the 3´-Linkers. (reply: 2)
Cloning short sequences - Does PCR can help clones upto 70 bps into a protein sequence (reply: 10)
plasmid cloning in E.coli - (reply: 2)
cloning problem - (reply: 1)
Cloning large fragment - Cloning (reply: 2)
trouble cloning into red vector - (reply: 1)
Problem in cloning lacZ in pUC18.lac- - ligation (reply: 2)
Cloning of synthetic oligos - how much oligos for ligation? (reply: 3)
miRNA effect on 3'UTR cloned into a reporter vector - (reply: 2)
Stable clone formation....please help - (reply: 2)
New to cloning...Help needed..DH5 alpha cells - Does Dh5alpha have antibiotic resistance to Amp? (reply: 3)
How to remove a few bases from my cloned insert using PCR - (reply: 3)
Blunt end cloning with Pfu and EcoRv - (reply: 3)
short DS-oligo cloning - (reply: 2)
Problem in cloning with electroporation. - (reply: 4)
Cloning incomplete genes - (reply: 2)
How to add a lethal gene to my plasmid for cloning selection? - (reply: 3)
TOPO cloning problem - (reply: 8)
subcloning help urgent! - (reply: 7)
Cloning help - how to break down a restriction site! (reply: 6)
Cloning after amplifying with Taq polymerase? - (reply: 5)
Cloning que - (reply: 2)
difficult cloning of large insert - (reply: 3)
cloning probelm - (reply: 1)
cloning artifact? - (reply: 4)
cloning through PCR - (reply: 3)
Cloning/Ligation: How not to have Insert/insert binding? - (reply: 5)
Cloning for Bisulfite sequencing - TOPO TA no results...... pGEM???? (reply: 2)
designing primers for cloning - designing primers for cloning (reply: 1)
blunt end ligation problem - (reply: 4)
cloning/ligation problem? (shRNA into vector) - cloning, colonies in neg control (reply: 4)
Cloning - (reply: 6)
cloning vs. direct sequencing - (reply: 1)
TA cloning - (reply: 6)
Screening of Mouse BAC Clone Libraries - Molecular Biology (reply: 2)
Ligation:blunt vs single base overhang - an old question? (reply: 5)
Cloning a cDNA sequence - (reply: 4)
Roche produce Blunt or dA tail PCR product? - (reply: 3)
Cloning & Expression - Cloning & Expression (reply: 7)
SubCloning binary vector - (reply: 5)
Quorum sensing gene cloning - (reply: 7)
make PCR template blunt ended - (reply: 2)
cloning mystery - numbers are perfect, but no insert (reply: 3)
how to select bacteria strain - about cloning (reply: 4)
Please helpI need a failsafe cloning method - (reply: 8)
cloning expression problem - (reply: 2)
Cloning problem - (reply: 9)
Ligation of half blunt PCR fragments - (reply: 4)
cloning primers, TM, and specificity - (reply: 4)
Directional cloning - (reply: 4)
Cloning problems into a big (19 kb) vector - (reply: 10)
cloning problem - (reply: 10)
Cloning a promoter sequence from genomic dna - (reply: 3)
Clone 2kb and 5 kb inserts into 6kb vector - Which order and vector:insert ration? (reply: 3)
Designing forward primer incorporating Nde1 cut site - Tips on cloning with Nde1? (reply: 2)
Strategies for gene cloning and expression - Discuss various methods for it (reply: 8)
Annealing and cloning of four single stranded oligos - (reply: 1)
PCR cloning problem - (reply: 6)
Trouble with cloning specific miRNA and mRNA 3' termini? - (reply: 3)
PCR cloning, faint band - (reply: 4)
TA Cloning problems - (reply: 11)
BAC Clone Extraction. - (reply: 3)
Stable Clone establishment using Hygromycin or Puromycin vectors - (reply: 3)
PCR cloning strategy - (reply: 4)
strange sequencing / cloning problem with AvrII-site - (reply: 2)
cloning/restriction analyses MAJOR PROBLEM - (reply: 2)
Cloning 5kb into PBlueScript (2.9kb)? - (reply: 5)
Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
Top 10 vs DH5α? - TOPO TA cloning question … (reply: 4)
Not sure with cloning - (reply: 2)
Fusion PCR for eventual TA cloning - (reply: 2)
long size cDNA cloning - (reply: 1)
Which enzyme is to be digested first in sequential RE? - In vector preparation for cloning... (reply: 7)
cloning size - plasmid (reply: 2)
Help with Cloning into LARGE vector - (reply: 11)
Help w/ CLONING...lots of transformants but no insert... - (reply: 8)
Cloning of gene from peptide sequence - (reply: 1)
which expert have cloned grp78 gene - (reply: 3)
Has anyone else encounted problems in cloning with PET-22b vector? - the plasmid even smaller than the empty PET-22b vector! (reply: 1)
cloning large inserts in large vectors - (reply: 4)
2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)
klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble - (reply: 5)
cloning excised product in pGBKT7 doesn't work! - (reply: 3)
very easy sticky end ligation: no colonies! - Cloning masters, please help doomed cloner! (reply: 28)
Water and cloning? - Major cloning disfunction... (reply: 18)
pcr-product cloning problems - cloning of microsatellites (reply: 4)
TA cloning help - (reply: 11)
shorter vector fragment at the end of the cloning - (reply: 8)
Clone two pieces of inserts - (reply: 6)
Topo TA cloning kit with Top 10 cells - Topo TA cloning kit with Top 10 cells (reply: 4)
More PCR cloning problems! - (reply: 12)
How much PCR do I need for cloning? - (reply: 5)
Cloning tips - (reply: 7)
PCR cloning problems - (reply: 13)
CIP of Vector and Kinase of Insert? - Is it necessary in blunt end cloning? (reply: 1)
TOPO cloning problems - (reply: 8)
is my cloning strategy right - (reply: 6)
cloning, got conoly, no dna at all. help! - (reply: 9)
blunt end ligation after inverse PCR - (reply: 5)
Cloning fragments with blunt ends - (reply: 2)
RACE product cloning in TOPO vector - (reply: 1)
TA cloning and mutation - (reply: 3)
Cloning of a Large Insert into a Lentiviral Vector - (reply: 5)
subcloning dengue NS1 gene problems - molecular biology, cloning (reply: 5)
Plasmid stability/Cloning problem - (reply: 3)
Cloning Problem - (reply: 2)
TA cloning help - (reply: 4)
my experience with cloning - (reply: 2)
question for the PCR to cloning construct - (reply: 9)
so how do blunt ends stick together? - (reply: 4)
RE digest and klenow - sequential blunting (reply: 6)
Can you flag-tag your sequence by PCR? - cloning shortcut (reply: 7)
Cloning Oligos - (reply: 2)
Cloning vector - which is good? (reply: 1)
Cloning and Expression vectors - Which is the best Expression vector to use? (reply: 3)
cut-delete-insert once more - just cloning? - (reply: 3)
inclusion of translation start site when cloning promoter insert - (reply: 3)
digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
Double Blunt Ended Ligation? - (reply: 8)
possible contamination of TA cloning vector? - (reply: 4)
Contamination with TA cloning vector? - (reply: 1)
Ecoli genomic recombination causing cloning problems - after ligation vector size and sequence change (reply: 5)
pGEM T easy vector for cloning - (reply: 29)
cloning of Poly A tail - (reply: 2)
one blunt and one sticky ligation...something wrong - HELP!!! (reply: 2)
help in cloning the promoter region of a gene - (reply: 3)
TOPO TA CLONING KIT - (reply: 13)
Cloning of truncated protein. - Do I need to include the stop codon? (reply: 11)
blunt end cloning - please help me understand this... (reply: 17)
Directional cloning failed again and again, what happened? - (reply: 8)
Possible to clone when you have only 20ng PCR product? - (reply: 10)
TOPO cloning always gets wrong orientation - (reply: 3)
pBluescript cloning of PCR products - (reply: 1)
Ligating blunt adapter to PCR product - (reply: 7)
Cloning Construction of 9000bp vector with 900bp - (reply: 14)
PCR clone a gene with known 3' end only - (reply: 4)
PCR Cloning - (reply: 3)
Request for Vector - gene cloning for DNA vaccine (reply: 1)
Few colonies with cloning of PCR product after digestion - (reply: 7)
subclone a double-cut insert - (reply: 4)
Molecular cloning of SalI/PacI digested product in modified pBluescript backbone - (reply: 4)
Cloning WITHOUT Topoisomerase! - Does any one know of an expression vector that does not depend on Topoisomerase (reply: 13)
cloning problem with pcr and pegfp vector - (reply: 9)
Should I clone my PCR product even though it is invisible on gel? - (reply: 5)
problems with cloning into a pET15b vector - (reply: 2)
TOPO CLONING - (reply: 5)
cloning of promoter - (reply: 2)
Problem with cloning p53-suggestions for RT-PCR - (reply: 1)
false positives when cloning microsatellite fragments - please help! - (reply: 3)
Replacing of GFP cloned inside a gene with RFP - (reply: 2)
Cloning into expression vector - Why doesn't it work anymore? (reply: 7)
pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
Ligation and cloning failure - (reply: 3)
Do I have to purify vector after RE digestion for cloning? - (reply: 17)
Sequence error in cloned expressed gene - how to correct it? - (reply: 15)
how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
can I detect colony for the insert with the cloning primers? - or clamp sequence will interfere? (reply: 4)
Ligation and Cloning Problem - Cloing Problem with pBSK (reply: 1)
Cloning diluted DNA - Confused!!!! (reply: 3)
cloning - ligation and cloning problems!!HELPPPPPPP (reply: 3)
Subcloning problem with XhoI digested fragment - weird pattern of migration/digestion after miniprep (reply: 6)
seeking cloning info - (reply: 9)
Cloning a very small insert into a "giant" vector! - (reply: 8)
cDNA synthesis for cloning - (reply: 3)
subclone help - (reply: 2)
Suggestions on Research design re: Cloning the promoter region and measure induc - Molecular genetics, protocol (reply: 7)
cloning primer and tag questions - first try (reply: 2)
Quick question: splitting TOPO cloning kit - (reply: 7)
shRNA cloning - (reply: 4)
Cloning questions - (reply: 2)
Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
TOPO directional cloning kit problems - (reply: 7)
klenow fill in protocol for making blunt end - (reply: 5)
Cloning - Problems about cloning (reply: 2)
obtaining cell clone? - (reply: 3)
how to clone blunt end and sticky end simultaneously? - (reply: 6)
Topo Zero Blunt Vector by Promega Amplification of insert with M13 - Amplification of insert with M13 primers (reply: 1)
Cloning Hell - problems with aquiring insert into vector (reply: 5)
To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
Cloning PCR product - Any ideas? (reply: 3)
cloning in a long vector (~10kb) without sequence changes - (reply: 2)
cloning vectors - limited insert size (reply: 10)
TA cloning...why is there no plasmid for BLUE colonies? - (reply: 2)
Blunt end ligation help - (reply: 2)
double-digest cloning problem - cloning (reply: 4)
Can pfu Polymerase make a blunt end? - (reply: 3)
Subcloning Problem - (reply: 6)
Plasmid map of the clone with APE or DNA Strider - (reply: 2)
cloning two fragments with deletion in between - PCR primer design (reply: 3)
Clone confirmation - (reply: 3)
t/a cloning - (reply: 1)
Cloning GC Rich Linker - PCR (reply: 3)
gateway system for cloning - (reply: 11)
why do cloning before sequencing - (reply: 8)
Making blunt and stick end vector - (reply: 2)
Cloning a PCR product after digestion - (reply: 7)
Cloning of long gene - (reply: 1)
siRNA Cloning method - (reply: 2)
gateway cloning trouble: a lot of false positives colonies - (reply: 3)
Problems in cloning shRNA - (reply: 13)
cloning restriction fragment for sequencing - (reply: 1)
Cloning with real time PCR fragments - using SyberGreen from Applied Biosystem (reply: 3)
Many colonies but no positive clone - (reply: 1)
converting overhang into blunt end - can i use Pfu instead Klenow? (reply: 3)
pGEM cloning blues - PCR cloning worked but blank and +control did'nt (reply: 2)
Site directed mutagenesis - wild type clone (reply: 4)
Cloning - intronic sequences needed? - (reply: 4)
sticky-end cloning problem? - (reply: 10)
stick end cloning problem? - (reply: 1)
How to clone enhancer sequence - (reply: 1)
hurry! how can I clone large DNA fragment - noboday answer? (reply: 1)
No Colonies on subcloning - (reply: 11)
Taq/PFU polymerase A overhang - bad for blunt ending? (reply: 3)
Degradation of PCR product during purfication - Purification of PCR product for cloning (reply: 2)
BAC clone amplification - (reply: 1)
Need cloning program - (reply: 10)
Non-directional cloning - (reply: 5)
problems in subcloning PCR products for TA cloning - (reply: 4)
cloning membrane protein with 2 tags - (reply: 2)
need help for Topo cloning - (reply: 6)
No success with PCR cloning of a gene into expression vector - (reply: 5)
problem cloning 2.5 kb fragment - (reply: 2)
ligation with sticky and blunt ends - (reply: 3)
directional cloning - (reply: 1)
Insertions/deletions at cloning restriction site - (reply: 1)
cloning a fragment using RE sites - protocol given really fuzzy. (reply: 4)
long insert DNA TA clone problem,help me ! urgent! - (reply: 2)
HELP! cloning cDNA from double-stranded cDNA synthesis - (reply: 2)
TA cloning: if PCR product is not freshly prepared - (reply: 2)
Directional cloning problem - (reply: 2)
Blunting with klenow fragment - (reply: 2)
Once more... cloning of a 13 kb fragment into pBR322 - (reply: 2)
Molecular Cloning: UV Damage - (reply: 4)
cloning the promoter from genomic dna - (reply: 9)
single clone isolate showed multiple bands in DGGE - (reply: 1)
What'sa phagemid? - phagemid cloning (reply: 3)
Cloning Problem - (reply: 5)
Cloning gene behind GFP - Vector? (reply: 2)
Cloning and Charecterization of a Novel Receptor - (reply: 1)
siRNA stable clone problem - protein level not reduced, but did have impact on cell function! (reply: 2)
cloning from e.coli in e.coli - (reply: 4)
Getting the right sequence by cloning - (reply: 7)
Interpreting RE digest results after blunt cloning - (reply: 1)
cloning/amplifying attenuate RNA transcripts - (reply: 6)
PCR cloning problem - No colonies - (reply: 10)
blunting with no success! - blunt end ligation (reply: 1)
TOPO TA cloning - (reply: 2)
Huge problem in cloning, need help quite urgent - (reply: 2)
Blunt end cloning options - (reply: 1)
topo XL cloning - (reply: 5)
blunt end ligation - Help (reply: 11)
tricky digestion for cloning gurus - (reply: 13)
Cloning primer-dimer fragments - (reply: 2)
Xba I and subcloning - (reply: 10)
PCR or digestions for clone screen? - (reply: 5)
KpnI Ligation Mystery - Bidirectional Cloning Working Unidirectionally (reply: 5)
stange cloning results - pBluescript seeming to double up (reply: 6)
what competent cells should I use to clone big size DNA - (reply: 8)
Where to buy a full length clone? - (reply: 3)
shorter PCR products after bisulfite treatment and cloning - (reply: 5)
Blunt PCR cloning not working - (reply: 2)
question for all you cloning gurus... - (reply: 8)
Cloning a 2kb fragment! - (reply: 2)
cloning 11 kb fragment - (reply: 2)
cloning vector - cloning vector (reply: 1)
which vector will be best for cloning and then expression - vector (reply: 1)
Plz help me troubleshoot subcloning! - (reply: 6)
Blunt ended ligation - (reply: 7)
So frustrated... a question about blunt end cloning - (reply: 6)
I feel desperate about cloning, who can help me? - (reply: 12)
BamHI/XbaI cloning, bad combination? - (reply: 3)
TA Cloning - (reply: 4)
Has anyone used In-Fusion Cloning Kit? - (reply: 3)
cloning of big insert(6.4Kb) in pUC18/19 - (reply: 2)
Problems cloning into pET Kanamycin resistance vectors - (reply: 7)
Cloning - how to avoid religation and no insert? - (reply: 13)
degradation of cloned protein in E. coli - (reply: 2)
deletion during cloning - (reply: 3)
gene clone - (reply: 1)
No insert for cloning PCR product into expression vector - (reply: 5)
Ligation help ! - blunt end ligation probs. (reply: 4)
TOPO cloning reaction transformation after 2 days - (reply: 1)
cloning problems - help! (reply: 4)
rabbit intestinal cDNA - Full length cloning (reply: 1)
Cloning PCR product - double bands or heterozygote (reply: 5)
cloning systems - pGEM-T easy vector system versus TOPO TA cloning? (reply: 4)
blunt ended cloning problem - (reply: 7)
How to purify and clone a 88 bp fragment? - (reply: 4)
cloning of PCR product - (reply: 1)
Strange things in clone - (reply: 6)
cDNA cloning question - (reply: 7)
Many Problems with Cloning - (reply: 2)
Cloning using RT-PCR for long fragment of 6kb - (reply: 1)
T4 blunting follow by digestion - (reply: 2)
Allele specific cloning efficiency after bisulfite treatment - (reply: 4)
yeast contamination - problem in cloning (reply: 4)
help pcr cloning problem - help pcr cloning problem!!!!!1 (reply: 2)
Cloning in old days - (reply: 2)
Cloned genes - (reply: 1)
FLAG Peptide how much to use w/M2 clone - competition w/M2 clone for immunocyto (reply: 1)
topo cloning for sequencing? - (reply: 3)
Problem with directional cloning - Problem with directional cloning (reply: 8)
ACK! Blunt end cloning - I'm confused.. - (reply: 2)
question about PCR clone a cDNA - (reply: 9)
Strange cloning pattern - Vector seems to religate with incompatible ends (reply: 8)
Whole gene cloning - (reply: 2)
cDNA cloning problem - No product in PCR (reply: 5)
Quick cloning question - Purification of PCR fragment (reply: 1)
Directional cloning and clone mutations - each clone has a different mutation! (reply: 1)
Cloning issue - vector insert ratio - (reply: 3)
one end blunt end and other end sticky end-- ligation - vector with one end sticky and other blunt end (reply: 13)
subcloning fragments - cloning (reply: 5)
cloning large gene - advice (reply: 2)
stupid redasoft visual cloning - (reply: 4)
How to predict a promoter region for a gene - Promoter cloning + genes into vector (reply: 6)
Validity of sequences via TA cloning - (reply: 1)
TA cloning Vector - ...how to prepare it myself (reply: 1)
cloning problems - cloning problems (reply: 8)
Negative controls in cloning? - (reply: 4)
Sticky ends to blunt - (reply: 5)
quick cloning question - (reply: 3)
problems cloning into plenti6/V5 lentiviral vector - (reply: 2)
TOPO TA Vector Cloning problem - no colonies - (reply: 5)
Problem with subcloning - (reply: 3)
help needed for large construct cloning - (reply: 6)
Cloning difficulty - Problems subcloning a very large fragment... (reply: 6)
trouble with TOPO TA CLONING KIT for SEQUENCING - (reply: 8)
Two cloning and expression problems - (reply: 2)
cloning shRNA - help (reply: 3)
cloning problem - sticky end cloning with PCR insert - (reply: 3)
Experience with fusion cloning system? - (reply: 8)
how do i analyse cloned sequences - Clone and DNA sequence (reply: 12)
cloning question - sticky ends - has anyone tried this and would it work? (reply: 3)
Another sub- cloning protocol not working - Mol. Biol. (reply: 1)
Gateway Blunt end cloning - no colonies at all (reply: 1)
TOPO XL cloning with 7.5kb PCR product - troubleshooting help (reply: 1)
Cloning large pcr product - efficiency (reply: 2)
One Step RT-PCR cloning problem - (reply: 2)
cloning with rare 8-cutters - (reply: 2)
TA cloning problem - (reply: 5)
Help a first time cloner with PCR! - First time cloning (reply: 5)
subcloning - (reply: 7)
Mysterious gain in vector size after cloning! - (reply: 1)
Cloning and PCR Problems - (reply: 1)
Problem with cloning - can't clone a 3.2kb fragment (reply: 5)
overlap extension and clone gene into pET -15b - a question?? (reply: 3)
Cloning of sequence with PCR added sites - BstBI and BclI (reply: 2)
A tried and true cloning protocol is FAILING miserably! - Molecular biology (reply: 6)
Confusion with cloning strategy - (reply: 3)
T/A cloning of cDNA? - PGEM-T vs pCR 2.1 T/A TOPO? (reply: 3)
Trouble cloning a 8.5 kb fragment into a pTRE vector - (reply: 2)
Cloning a big insert - insert size (reply: 1)
Rolling circle replicating plasmid and transformation - Gene cloning (reply: 2)
subcloning into a single NotI site - (reply: 11)
Cloning a gene without the sequence of the target species - (reply: 3)
subcloning of PCR product, please help! - subcloning problems (reply: 12)
cloning primer orientation - orientation and complement of primers in a cloning (reply: 3)
phosphatase alkaline before cloning? - (reply: 3)
cloning genes with incompatible restriction sites - Protein expression (reply: 2)
How would you identify and clone quinine transporters for P. - (reply: 1)
importance of directionality in cloning - (reply: 1)
Problems cloning cDNA using Pfx - Problem (reply: 2)
difficulty in cloning oligos into vector - cloning difficulties (reply: 1)
Topo cloning no colonies - (reply: 3)
cloning tricks - (reply: 2)
Cloning RACE products - (reply: 1)
Contamination in PCR cloning - (reply: 3)
Blunt End Ligation !Which god to pray? - (reply: 2)
Cloning a large PCR product - trouble cloning large DNA (reply: 3)
double sized PCR product from cloned cDNA - (reply: 6)
Is kozak sequence necessary for cloning? - (reply: 4)
problem in cloning work - (reply: 7)
TA cloning with ABgene's extensor HiFi polymerase - (reply: 1)
Screening of TA cloning by T7/Sp6 PCR possible? - (reply: 2)
Cloning from scratch, please help... - (reply: 3)
difficulty about cloning 3.5kb PCR product - (reply: 1)
EcoRI cloning - correct orientation - Preferential orientation? (reply: 3)
CIPping after Klenow blunting - Is CIP necessary in Blunt end ligation? (reply: 2)
TA cloning with short PCR products - use of TA cloning kit (reply: 4)
Cloning, ligation, transformation with pET - Problem re-ligating with NotI (reply: 1)
Cloning into pUAST - Help needed (reply: 4)
Promoter Cloning - PCR amplification of a promoter and cloning (reply: 1)
Problem with TA PCR cloning - no insert in white colonies - (reply: 12)
cloning problems - (reply: 1)
cDNA cloning by PCR - cloning (reply: 3)
TOPO TA pCR 2.1 cloning - (reply: 1)
Blunting both vector and insert - klenow and CIPping (reply: 2)
Question about cloning a gene coding an enzyme - (reply: 1)
Cloning rare splice variants - Molecular Biology (reply: 1)
Expression cloning system for protease expression - (reply: 2)
Cloning Large Fragments in a pool of DNA fragments - (reply: 1)
Large insert cloning - (reply: 1)
Which is better? To blunt my insert and vector or use linkers? - To insert the 3.5 kb insert cut with EcoRI and SalI in to a (reply: 1)
How big DNA fragment can I clone to pUC19 plasmid? - (reply: 2)
How important is cloning of full-length genes? - (reply: 1)
mat agar dish and a cloning problem - (reply: 4)
BAC cloning problems - (reply: 2)
blunt end ligation problems - (reply: 6)
TA cloning - (reply: 5)
Cloning problem - Insert DNA self-ligation? - (reply: 11)
Which vector for cloning big PCR fragments? - (reply: 3)
Regarding smaI restriction enzyme - Blunt end cloning (reply: 5)
PCR Ligation cloning - (reply: 2)
Problem with cloning by dephospharylation - Problem in cloning (reply: 4)
cloning in pQE30 - (reply: 3)
cloning big fragments - (reply: 1)
Cloning problem - Digestion, ligation and transformation (reply: 6)
Cloning into shRNA vectors - (reply: 4)
Colony PCR for clone screen - Colony PCR before sending sequence (reply: 4)
Cloning into GFP vector - (reply: 2)
cloning of long viral fragment I - (reply: 2)
TOPO-TA Cloning PCR product - Is amplicilin selection engough? (reply: 4)
Cloning kit recommendations? - advice sought (reply: 2)
pET100 TOPO cloning kit sucks - (reply: 2)
cloning - (reply: 2)
why so many small inserts? - puzzles in cloning (reply: 7)
where to buy sterile grease used for isolate cell clone? - (reply: 2)
TA cloning question - (reply: 2)
Cloning pCAEGFP into XL1 E. coli - I cannot get any colonies (reply: 2)
Cloning Isoforms - (reply: 1)
cloning problem - cloning problem in GFP vector (reply: 4)
Problem cloning PCR product into double-cut vector - (reply: 3)
TA cloning -- funny sized bands - (reply: 1)
Clone blunt ended insulin gene into pbr322 - help (reply: 3)
Subcloning into large plasmid - (reply: 3)
TA cloning problems - (reply: 2)
Cloning RACE products - Problem with sequencing ends (reply: 3)
Primer design - PCR for cloning (reply: 6)
gateway cloning problems - (reply: 2)
1-2kb PCR products TOPO cloning troubles - help (reply: 4)
RT-PCR for cloning low expressed genes - RT-PCR for cloning low expressed genes (reply: 1)
T- vector clone - (reply: 3)
Subcloning into lentiviral vectors - Very little to no plasmid after miniprep (reply: 7)
PCR Cloning - Please Help - Urgent - Cloning using SalI & BamHI site (reply: 1)
Cloning 10 Kb Fragment - (reply: 1)
TA cloning kit problem - No single colonies after transformation!!! (reply: 10)
cloning PCR fragment - PCR fragment is not getting cloned in the vector (reply: 2)
Simple Ligation/cloning questions - question on dNTPs and ligation protocol (reply: 1)
"Blunting" - (reply: 6)
Cloning a long PCR product - (reply: 2)
Problem in PCR cloning - (reply: 7)
cloning size is not correct - 2 bands on the PCR product (reply: 2)
still more about cloning PCR products - (reply: 9)
large fragment cloning - (reply: 2)
Cloning long fragments - (reply: 18)
plasmid draw - Need software for Vector cloning design (reply: 4)
cloning from PCR - (reply: 25)
PCR cloning - PCR cloning (reply: 2)
cloning upper stream of a gene - (reply: 1)
help - stable transfection clone pick up - (reply: 33)
Cloning using ds Oligos - concentration, phosphorylate? (reply: 2)
Cloning Problems - #@%$&*^) (reply: 5)
any experience in cloning a 3.5kb full length cDNA - (reply: 4)
Help me - gene subclone (reply: 3)
clone - how to clone an unknown gene (reply: 1)
PCR Cloning - (reply: 1)
Subcloning transgene from a BAC clone - Subcloning transgene from a BAC clone (reply: 1)
Problem with cloning unstable HIV insert - (reply: 1)
I need a little help with TA cloning - TA cloning (reply: 1)
cloning of lacZ - i can't clone the lac Z!! (reply: 1)
Cloning of high-GC% gene into vector - (reply: 3)
why the clone ware so small? - plamid transformation (reply: 1)
Selection of StableTransfectants Expressing a High Density of Cloned M - (reply: 1)
Cloning antibodies - (reply: 1)
Any experience in improving blunt end ligation? - (reply: 6)
subcloning - (reply: 5)
Subcloning problem - pCR4-Topo -> pcDNA3 (reply: 1)
Blunting 3' ends with T4 polymerase - (reply: 1)
oligo cloning - (reply: 2)
How can I clone the promoter region of my gene - (reply: 3)
Help please!!! - Delection after cloning (reply: 1)
Subcloning - (reply: 2)
cloning question - (reply: 2)
cloning 60nt Oligos - Help (reply: 1)
ligation problems - cloning a PCR fragment - into a double-cut vector (reply: 4)
clone Src and Ras - (reply: 1)
7kb clone trouble - topo (reply: 1)
Sticky Problem to a Blunt end - Cloning (reply: 1)
cloning of pcr product - (reply: 2)
topo TA cloning kit (invitrogen) - URGENT (reply: 5)
How to clone large DNA fragment into small vector! - (reply: 1)
Deletion after cloning - (reply: 2)
Cloning error prone PCR products - (reply: 1)
How can I clone the upstream or downstraem of my gene?? - How can I clone the upstream or downstraem of my gene?? (reply: 1)
Re: PCR cloning problem - (reply: 1)
why the clone grow so slowly - (reply: 1)