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Cloning Related Discussions
  1. Cloning from genomic DNA - (reply: 1)
  2. Promoter cloning... - Problems (reply: 2)
  3. Blunt end ligation - (reply: 4)
  4. Zero Blunt cloning - ligation problem? (reply: 1)
  5. Possible to add a linker to only one blunt end? - (reply: 4)
  6. help on directional cloning - (reply: 3)
  7. Problems during plasmid prep in cloning - (reply: 6)
  8. Release of an insert from the cloning vector using restriction enzymes - (reply: 1)
  9. pSUPERIOR cloning - please help! - (reply: 1)
  10. cloning into the pBAD-GFPuv - (reply: 1)
  11. commercial TA cloning vector with Luciferase - cloning promoter sequences (reply: 2)
  12. Cloning 1.5kB gene into lentivirus--I'm stumped - (reply: 2)
  13. Problems subcloning with the pL452 recombineering plasmid - (reply: 2)
  14. Xba I subcloning problem - (reply: 6)
  15. cloning kills - (reply: 5)
  16. Why do I get only white colonies that do not grow in the midst liquid? - Troubes with cloning in pGEM-T (reply: 6)
  17. TOPO TA Cloning - hundreds of colonies, 0 insert - (reply: 6)
  18. M13 for cloning? - (reply: 4)
  19. Better Molecular Biology Reference: Molecular Cloning vs. Short Protocols - (reply: 1)
  20. cloning pGEMT - (reply: 4)
  21. sequential PCR and purification before TA cloning - (reply: 1)
  22. problem with cloning using pTYB2 - (reply: 2)
  23. how to clone the promoter of a gene - (reply: 3)
  24. genome amplification before cloning? - (reply: 2)
  25. how to clone multiple inserts simulataneously - need to make a triple fusion (reply: 1)
  26. cloning into plasmid (IMPACT kit from NEB) - (reply: 3)
  27. cloning and transformation - (reply: 1)
  28. ta cloning vectors - non commercial ta cloning vectors (reply: 1)
  29. how to clone unstable DNA? - (reply: 4)
  30. miRNA - cloning strategies - (reply: 2)
  31. Techanical Question about cloning of gene - (reply: 2)
  32. How to test if a cDNA clone from a plasmid is infectious? - (reply: 4)
  33. Cloning of genes - (reply: 2)
  34. DNA polymerase that produces blunt end and doesnt smear! - (reply: 1)
  35. cloning of alternative splicing variants - (reply: 2)
  36. Cloning in frame into pcDNA 3.1(+) vector - a quick question (reply: 6)
  37. QUestion on subcloning a 80bp product from TOPO vector - (reply: 5)
  38. Positional Cloning - (reply: 1)
  39. TA cloning, sequencing results are vector self-ligation while bacterial PCR iden - (reply: 2)
  40. RE cloning a 2.3kb fragment into a 4.7kb vector - (reply: 4)
  41. Please help me analyze BSP cloned sequencing results. - (reply: 8)
  42. How can I create a T vector for PCR product cloning - (reply: 2)
  43. Help: PCR clone problem - (reply: 2)
  44. Question on transcription start site and promoter cloning - (reply: 3)
  45. Help in Clone Expression - Trasformants grow on plate but not in medium (reply: 2)
  46. design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
  47. Retroviral vector cloning help - (reply: 6)
  48. cloning with pGEM T-easy vector - question about the vector. (reply: 2)
  49. Sequencing:why do pcr AND cloning into plasmid? - (reply: 6)
  50. CLONING LONG INSERT RATIO INS:VEC - (reply: 4)
  51. Please comment on this simple cloning strategy - (reply: 6)
  52. too many cloning false positives - (reply: 5)
  53. How about vector sequence in cloning? - (reply: 9)
  54. Cloning Problem ! - (reply: 6)
  55. Cloned genes dont express! - (reply: 6)
  56. Stable cell line - clone vs. population - (reply: 15)
  57. Will TOPO TA cloning work on "old" purified PCR product? - (reply: 4)
  58. Do I need to clone into gateway vector or pGEMT easy vector before cloning into - (reply: 1)
  59. how to prepare c dna for cloning - (reply: 1)
  60. RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
  61. Cloning and Sequencing woes - (reply: 9)
  62. cloning in pNL4-3 - (reply: 1)
  63. Morphology of ES cell clones - Does this clone look like ES cells? (reply: 4)
  64. Sal I cloning problems - (reply: 13)
  65. cloning of 40 nucleotide repeat fragments - (reply: 4)
  66. Ligation problem with PCR product cloning - (reply: 9)
  67. problems in clone confirmation - (reply: 3)
  68. large fragment cloning problem! Help needed - (reply: 2)
  69. Cloning trouble - (reply: 7)
  70. cloning - (reply: 2)
  71. PCR product cloning - (reply: 6)
  72. Stability and degradation of cloning reagents - May help resolve problematic cloning (reply: 1)
  73. how to clone the genomic region for coding the mature miRNA - (reply: 2)
  74. problem with bi-enzyme cloning - (reply: 4)
  75. Calculating protein size - how to calculate the protein size when cloned in pet vector (reply: 1)
  76. Blunt end cloning question - (reply: 1)
  77. Molecular Cloning: A Laboratory Manual (3-Volume Set) - buy now or later? (reply: 4)
  78. Double ligation - What about cloning two fragments in the same reaction? (reply: 5)
  79. Blunt-end/cohesive ends cloning - (reply: 7)
  80. cloning - (reply: 9)
  81. gateway cloning - (reply: 1)
  82. Bisulfite direct sequencing + cloning - (reply: 2)
  83. cloning - (reply: 1)
  84. Help with TOPO TA cloning – too long or too many insert - (reply: 1)
  85. Cloning big inserts - (reply: 4)
  86. cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector?? (reply: 2)
  87. plasmid subcloning - (reply: 1)
  88. Random Amplification + TA cloning = HATE - (reply: 2)
  89. how important a terminator is in cloning? - (reply: 3)
  90. Cloning 2kb+ gene by PCR - Is it doable? (reply: 3)
  91. strange transformation products in subclone - (reply: 4)
  92. BLUNT END LIGATION - DIFFICULTIES ON BLUNT END LIGATIONS (reply: 3)
  93. sticky/blunt end cloning failure - (reply: 1)
  94. cloning problem - (reply: 2)
  95. Blunt end cloning - vector and insert preparation (reply: 9)
  96. Blunt or sticky ligation-Which is more efficient? - (reply: 3)
  97. E. coli stain for cloning that grow up very quick - Any one who could give the info??? (reply: 5)
  98. cDNA cloning using Taq/pfu polymerase - (reply: 2)
  99. lentiviral vector cloning problem - (reply: 1)
  100. Using mRNA in cloning - (reply: 1)
  101. compare pEGM T easy vector and TOPO TA cloning - (reply: 7)
  102. Blunt ended ligation not working! - (reply: 3)
  103. Problem in cloning - (reply: 8)
  104. pros and cons of Blunt vs sticky ligation - (reply: 2)
  105. Blunt end ligation problems, no ligation at all... - (reply: 2)
  106. How can clone the complete miRNA primary transcript ? - (reply: 2)
  107. primer design for subclone - (reply: 2)
  108. Problems cloning a tiny insert into a huge vector - (reply: 1)
  109. Problems with cloning HIV env gene - HIV cloning (reply: 4)
  110. Blunt end vector........? - (reply: 2)
  111. Cloning with BAC - (reply: 2)
  112. cloning problem - (reply: 1)
  113. My DNA fragment in agarose gel is in 4 degree - Can I extract the fragment and use it for cloning??? (reply: 6)
  114. cloning, ligation, dephosphorylation - ligation, dephosphorylation (reply: 3)
  115. unable to clone into pET29b and pET32b - sticky end ligation failing (reply: 2)
  116. subcloning into pET21b failing - (reply: 7)
  117. What exactly does it mean by cloning a toxic gene - (reply: 2)
  118. cloning 708bp PCR prodyct into pET101 vector (TOPO) - (reply: 2)
  119. shotgun cloning: gel shows inserts but only plasmid sequence obtained - (reply: 1)
  120. Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
  121. cloning a 3kb fragment into a 6kb vector - or maybe an EcoRI/HindIII double digest problem? (reply: 3)
  122. route map for cloning a gene - steps involved in subcloning a gene (reply: 3)
  123. Cloning toxic gene - (reply: 2)
  124. Cloning PCR product blunt + restriction site - (reply: 2)
  125. help in cloning 6kb pcr fragment into topo? - (reply: 5)
  126. problem with cloning and sequencing. - (reply: 10)
  127. orientation in cloning - (reply: 1)
  128. Problems with TA cloning of bisulfite converted PCR product - (reply: 2)
  129. Cloning into GATEWAY system - (reply: 3)
  130. Need help with TOPO TA cloning problem! - TOPO TA clonig, ligation, no insert (reply: 23)
  131. cloning in expression vector - (reply: 5)
  132. Using single restriction enzyme SalI for cloning to a binary vector. How I will - Please its really very urgent...help me...help me.. (reply: 4)
  133. klenow/T4 question - Making one end blunt (reply: 4)
  134. Cloning of construct in SalI site - (reply: 3)
  135. Need help about my subcloning pET15b. - (reply: 1)
  136. primer design for PCR cloning - (reply: 1)
  137. opposite sequence orientation in TA cloning - (reply: 2)
  138. sticky and blunt DNA on gel migration - (reply: 5)
  139. sticky end to blunt end? - cloning (reply: 3)
  140. molecular cloning - TA cloning (reply: 3)
  141. Gateway cloning with 3 entry vectors - (reply: 4)
  142. Utter failure of directional cloning - need help - (reply: 5)
  143. cloning faint bands - (reply: 5)
  144. Is this clone the correct one? - Restriction digestion doubt (reply: 1)
  145. Problem with TA cloning - (reply: 1)
  146. Cloning using adaptors-->converting RE site - (reply: 4)
  147. how to ensure that insert orientation is correct in TA cloning - (reply: 6)
  148. Cloning problem: only frameshift mutants survive - (reply: 13)
  149. BAC vecor cloning - (reply: 3)
  150. AT-Rich Insert Cloning - (reply: 2)
  151. How to buy a cDNA clone? - (reply: 2)
  152. Why do you need your insert in the vector in both orientations? - Cloning (reply: 4)
  153. blunt end - filling, polishing (reply: 1)
  154. Creating short dsDNA from primers for cloning - (reply: 2)
  155. cloning of 1 kb fragment in 14 kb vector - (reply: 3)
  156. cloning problem ....... - (reply: 4)
  157. Cloning small DNA fragments. Bacteria are not growing - (reply: 5)
  158. cloning help about pBudCE4.1 - (reply: 1)
  159. cloning of a big Plasmid - (reply: 3)
  160. Loss of insert after cloning! - (reply: 3)
  161. cloning problem - (reply: 6)
  162. Blunt Cloning Large Insert/Smaller Vector - (reply: 9)
  163. PCR cloning: neg self-ligation control, many colonies but no insert - (reply: 3)
  164. Cloning Related Problem - Cloning in pBSK (reply: 1)
  165. Problems with directional cloning using BsmBI and Xba I - (reply: 6)
  166. Dephosphorylation for one cohesive, one blunt end ligation? - (reply: 1)
  167. what's the difference between sequencing primer and cloning one. - confused (reply: 5)
  168. Cloning trouble......help! - (reply: 4)
  169. cloning problem of big inserts - (reply: 4)
  170. how the multiple cloning site in cloning vector is create? - (reply: 4)
  171. cloning problem - (reply: 3)
  172. Cloning 50-100bp fragments. Need help. - i'm getting all blues... (reply: 10)
  173. can blunt & sticky ends stick - (reply: 11)
  174. help for no E coli. clone after transforming - (reply: 5)
  175. Cloning a 60 bp pcr product and Mixing Pfx and Taq polymerases - (reply: 4)
  176. PCR cloning problems - argh!!! (reply: 5)
  177. Cloning/Miniprep Blues - High molecular weight bands iand no plasmid n my miniprep (reply: 3)
  178. cloning shRNA into pLentilox 3.7 - (reply: 3)
  179. site after blunting - (reply: 1)
  180. Cloning w/pCR4 TOPO vector - problems obtaining clones (reply: 1)
  181. blunt ending the vector followed by the ligation - (reply: 1)
  182. Bisulfite sequencing - why cloning? - (reply: 3)
  183. Inability to grow plasmid in culture - Molecular cloning (reply: 3)
  184. Confused about cre-lox recombination system - Why is CreatorTM DNA cloning better over other cre-lox systems? (reply: 1)
  185. Trouble cloning miRNAs - (reply: 5)
  186. Problems with cloning - (reply: 4)
  187. Issues with pcDNA3.1Directional cloning - (reply: 2)
  188. Problem with cloning pGEMT easy vector system - (reply: 4)
  189. cDNA cloning - (reply: 2)
  190. polyA tail as 3' adaptor to clone miRNA - (reply: 1)
  191. Amplify RANKL for cloning - (reply: 3)
  192. Cloning with linkers? - (reply: 5)
  193. Blunt end cloning PCR product - (reply: 6)
  194. cloning impossible - (reply: 10)
  195. cloning cDNA libraries - (reply: 4)
  196. enzyme site after blunting - (reply: 7)
  197. How to thansfer the single clone - (reply: 2)
  198. Has any one successfully performed miRNA cloning? - miRNA cloning troubleshooting (reply: 4)
  199. integration vector question - do i need to clone a full gene (reply: 1)
  200. Quick ligation and blunting kits NEB - (reply: 7)
  201. Uridines in linker design for miRNA cloning. - Why uridine is often used in the 3´-Linkers. (reply: 2)
  202. Cloning short sequences - Does PCR can help clones upto 70 bps into a protein sequence (reply: 10)
  203. plasmid cloning in E.coli - (reply: 2)
  204. cloning problem - (reply: 1)
  205. Cloning large fragment - Cloning (reply: 2)
  206. trouble cloning into red vector - (reply: 1)
  207. Problem in cloning lacZ in pUC18.lac- - ligation (reply: 2)
  208. Cloning of synthetic oligos - how much oligos for ligation? (reply: 3)
  209. miRNA effect on 3'UTR cloned into a reporter vector - (reply: 2)
  210. Stable clone formation....please help - (reply: 2)
  211. New to cloning...Help needed..DH5 alpha cells - Does Dh5alpha have antibiotic resistance to Amp? (reply: 3)
  212. How to remove a few bases from my cloned insert using PCR - (reply: 3)
  213. Blunt end cloning with Pfu and EcoRv - (reply: 3)
  214. short DS-oligo cloning - (reply: 2)
  215. Problem in cloning with electroporation. - (reply: 4)
  216. Cloning incomplete genes - (reply: 2)
  217. How to add a lethal gene to my plasmid for cloning selection? - (reply: 3)
  218. TOPO cloning problem - (reply: 8)
  219. subcloning help urgent! - (reply: 7)
  220. Cloning help - how to break down a restriction site! (reply: 6)
  221. Cloning after amplifying with Taq polymerase? - (reply: 5)
  222. Cloning que - (reply: 2)
  223. difficult cloning of large insert - (reply: 3)
  224. cloning probelm - (reply: 1)
  225. cloning artifact? - (reply: 4)
  226. cloning through PCR - (reply: 3)
  227. Cloning/Ligation: How not to have Insert/insert binding? - (reply: 5)
  228. Cloning for Bisulfite sequencing - TOPO TA no results...... pGEM???? (reply: 2)
  229. designing primers for cloning - designing primers for cloning (reply: 1)
  230. blunt end ligation problem - (reply: 4)
  231. cloning/ligation problem? (shRNA into vector) - cloning, colonies in neg control (reply: 4)
  232. Cloning - (reply: 6)
  233. cloning vs. direct sequencing - (reply: 1)
  234. TA cloning - (reply: 6)
  235. Screening of Mouse BAC Clone Libraries - Molecular Biology (reply: 2)
  236. Ligation:blunt vs single base overhang - an old question? (reply: 5)
  237. Cloning a cDNA sequence - (reply: 4)
  238. Roche produce Blunt or dA tail PCR product? - (reply: 3)
  239. Cloning & Expression - Cloning & Expression (reply: 7)
  240. SubCloning binary vector - (reply: 5)
  241. Quorum sensing gene cloning - (reply: 7)
  242. make PCR template blunt ended - (reply: 2)
  243. cloning mystery - numbers are perfect, but no insert (reply: 3)
  244. how to select bacteria strain - about cloning (reply: 4)
  245. Please helpI need a failsafe cloning method - (reply: 8)
  246. cloning expression problem - (reply: 2)
  247. Cloning problem - (reply: 9)
  248. Ligation of half blunt PCR fragments - (reply: 4)
  249. cloning primers, TM, and specificity - (reply: 4)
  250. Directional cloning - (reply: 4)
  251. Cloning problems into a big (19 kb) vector - (reply: 10)
  252. cloning problem - (reply: 10)
  253. Cloning a promoter sequence from genomic dna - (reply: 3)
  254. Clone 2kb and 5 kb inserts into 6kb vector - Which order and vector:insert ration? (reply: 3)
  255. Designing forward primer incorporating Nde1 cut site - Tips on cloning with Nde1? (reply: 2)
  256. Strategies for gene cloning and expression - Discuss various methods for it (reply: 8)
  257. Annealing and cloning of four single stranded oligos - (reply: 1)
  258. PCR cloning problem - (reply: 6)
  259. Trouble with cloning specific miRNA and mRNA 3' termini? - (reply: 3)
  260. PCR cloning, faint band - (reply: 4)
  261. TA Cloning problems - (reply: 11)
  262. BAC Clone Extraction. - (reply: 3)
  263. Stable Clone establishment using Hygromycin or Puromycin vectors - (reply: 3)
  264. PCR cloning strategy - (reply: 4)
  265. strange sequencing / cloning problem with AvrII-site - (reply: 2)
  266. cloning/restriction analyses MAJOR PROBLEM - (reply: 2)
  267. Cloning 5kb into PBlueScript (2.9kb)? - (reply: 5)
  268. Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
  269. Top 10 vs DH5α? - TOPO TA cloning question … (reply: 4)
  270. Not sure with cloning - (reply: 2)
  271. Fusion PCR for eventual TA cloning - (reply: 2)
  272. long size cDNA cloning - (reply: 1)
  273. Which enzyme is to be digested first in sequential RE? - In vector preparation for cloning... (reply: 7)
  274. cloning size - plasmid (reply: 2)
  275. Help with Cloning into LARGE vector - (reply: 11)
  276. Help w/ CLONING...lots of transformants but no insert... - (reply: 8)
  277. Cloning of gene from peptide sequence - (reply: 1)
  278. which expert have cloned grp78 gene - (reply: 3)
  279. Has anyone else encounted problems in cloning with PET-22b vector? - the plasmid even smaller than the empty PET-22b vector! (reply: 1)
  280. cloning large inserts in large vectors - (reply: 4)
  281. 2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)
  282. klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble - (reply: 5)
  283. cloning excised product in pGBKT7 doesn't work! - (reply: 3)
  284. very easy sticky end ligation: no colonies! - Cloning masters, please help doomed cloner! (reply: 28)
  285. Water and cloning? - Major cloning disfunction... (reply: 18)
  286. pcr-product cloning problems - cloning of microsatellites (reply: 4)
  287. TA cloning help - (reply: 11)
  288. shorter vector fragment at the end of the cloning - (reply: 8)
  289. Clone two pieces of inserts - (reply: 6)
  290. Topo TA cloning kit with Top 10 cells - Topo TA cloning kit with Top 10 cells (reply: 4)
  291. More PCR cloning problems! - (reply: 12)
  292. How much PCR do I need for cloning? - (reply: 5)
  293. Cloning tips - (reply: 7)
  294. PCR cloning problems - (reply: 13)
  295. CIP of Vector and Kinase of Insert? - Is it necessary in blunt end cloning? (reply: 1)
  296. TOPO cloning problems - (reply: 8)
  297. is my cloning strategy right - (reply: 6)
  298. cloning, got conoly, no dna at all. help! - (reply: 9)
  299. blunt end ligation after inverse PCR - (reply: 5)
  300. Cloning fragments with blunt ends - (reply: 2)
  301. RACE product cloning in TOPO vector - (reply: 1)
  302. TA cloning and mutation - (reply: 3)
  303. Cloning of a Large Insert into a Lentiviral Vector - (reply: 5)
  304. subcloning dengue NS1 gene problems - molecular biology, cloning (reply: 5)
  305. Plasmid stability/Cloning problem - (reply: 3)
  306. Cloning Problem - (reply: 2)
  307. TA cloning help - (reply: 4)
  308. my experience with cloning - (reply: 2)
  309. question for the PCR to cloning construct - (reply: 9)
  310. so how do blunt ends stick together? - (reply: 4)
  311. RE digest and klenow - sequential blunting (reply: 6)
  312. Can you flag-tag your sequence by PCR? - cloning shortcut (reply: 7)
  313. Cloning Oligos - (reply: 2)
  314. Cloning vector - which is good? (reply: 1)
  315. Cloning and Expression vectors - Which is the best Expression vector to use? (reply: 3)
  316. cut-delete-insert once more - just cloning? - (reply: 3)
  317. inclusion of translation start site when cloning promoter insert - (reply: 3)
  318. digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
  319. cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
  320. Double Blunt Ended Ligation? - (reply: 8)
  321. possible contamination of TA cloning vector? - (reply: 4)
  322. Contamination with TA cloning vector? - (reply: 1)
  323. Ecoli genomic recombination causing cloning problems - after ligation vector size and sequence change (reply: 5)
  324. pGEM T easy vector for cloning - (reply: 29)
  325. cloning of Poly A tail - (reply: 2)
  326. one blunt and one sticky ligation...something wrong - HELP!!! (reply: 2)
  327. help in cloning the promoter region of a gene - (reply: 3)
  328. TOPO TA CLONING KIT - (reply: 13)
  329. Cloning of truncated protein. - Do I need to include the stop codon? (reply: 11)
  330. blunt end cloning - please help me understand this... (reply: 17)
  331. Directional cloning failed again and again, what happened? - (reply: 8)
  332. Possible to clone when you have only 20ng PCR product? - (reply: 10)
  333. TOPO cloning always gets wrong orientation - (reply: 3)
  334. pBluescript cloning of PCR products - (reply: 1)
  335. Ligating blunt adapter to PCR product - (reply: 7)
  336. Cloning Construction of 9000bp vector with 900bp - (reply: 14)
  337. PCR clone a gene with known 3' end only - (reply: 4)
  338. PCR Cloning - (reply: 3)
  339. Request for Vector - gene cloning for DNA vaccine (reply: 1)
  340. Few colonies with cloning of PCR product after digestion - (reply: 7)
  341. subclone a double-cut insert - (reply: 4)
  342. Molecular cloning of SalI/PacI digested product in modified pBluescript backbone - (reply: 4)
  343. Cloning WITHOUT Topoisomerase! - Does any one know of an expression vector that does not depend on Topoisomerase (reply: 13)
  344. cloning problem with pcr and pegfp vector - (reply: 9)
  345. Should I clone my PCR product even though it is invisible on gel? - (reply: 5)
  346. problems with cloning into a pET15b vector - (reply: 2)
  347. TOPO CLONING - (reply: 5)
  348. cloning of promoter - (reply: 2)
  349. Problem with cloning p53-suggestions for RT-PCR - (reply: 1)
  350. false positives when cloning microsatellite fragments - please help! - (reply: 3)
  351. Replacing of GFP cloned inside a gene with RFP - (reply: 2)
  352. Cloning into expression vector - Why doesn't it work anymore? (reply: 7)
  353. pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
  354. Ligation and cloning failure - (reply: 3)
  355. Do I have to purify vector after RE digestion for cloning? - (reply: 17)
  356. Sequence error in cloned expressed gene - how to correct it? - (reply: 15)
  357. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
  358. can I detect colony for the insert with the cloning primers? - or clamp sequence will interfere? (reply: 4)
  359. Ligation and Cloning Problem - Cloing Problem with pBSK (reply: 1)
  360. Cloning diluted DNA - Confused!!!! (reply: 3)
  361. cloning - ligation and cloning problems!!HELPPPPPPP (reply: 3)
  362. Subcloning problem with XhoI digested fragment - weird pattern of migration/digestion after miniprep (reply: 6)
  363. seeking cloning info - (reply: 9)
  364. Cloning a very small insert into a "giant" vector! - (reply: 8)
  365. cDNA synthesis for cloning - (reply: 3)
  366. subclone help - (reply: 2)
  367. Suggestions on Research design re: Cloning the promoter region and measure induc - Molecular genetics, protocol (reply: 7)
  368. cloning primer and tag questions - first try (reply: 2)
  369. Quick question: splitting TOPO cloning kit - (reply: 7)
  370. shRNA cloning - (reply: 4)
  371. Cloning questions - (reply: 2)
  372. Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
  373. TOPO directional cloning kit problems - (reply: 6)
  374. klenow fill in protocol for making blunt end - (reply: 3)
  375. Cloning - Problems about cloning (reply: 2)
  376. obtaining cell clone? - (reply: 3)
  377. how to clone blunt end and sticky end simultaneously? - (reply: 6)
  378. Topo Zero Blunt Vector by Promega Amplification of insert with M13 - Amplification of insert with M13 primers (reply: 1)
  379. Cloning Hell - problems with aquiring insert into vector (reply: 5)
  380. To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
  381. Cloning PCR product - Any ideas? (reply: 3)
  382. cloning in a long vector (~10kb) without sequence changes - (reply: 2)
  383. cloning vectors - limited insert size (reply: 10)
  384. TA cloning...why is there no plasmid for BLUE colonies? - (reply: 2)
  385. Blunt end ligation help - (reply: 2)
  386. double-digest cloning problem - cloning (reply: 4)
  387. Can pfu Polymerase make a blunt end? - (reply: 3)
  388. Subcloning Problem - (reply: 6)
  389. Plasmid map of the clone with APE or DNA Strider - (reply: 2)
  390. cloning two fragments with deletion in between - PCR primer design (reply: 3)
  391. Clone confirmation - (reply: 3)
  392. t/a cloning - (reply: 1)
  393. Cloning GC Rich Linker - PCR (reply: 3)
  394. gateway system for cloning - (reply: 11)
  395. why do cloning before sequencing - (reply: 8)
  396. Making blunt and stick end vector - (reply: 2)
  397. Cloning a PCR product after digestion - (reply: 7)
  398. Cloning of long gene - (reply: 1)
  399. siRNA Cloning method - (reply: 2)
  400. gateway cloning trouble: a lot of false positives colonies - (reply: 3)
  401. Problems in cloning shRNA - (reply: 13)
  402. cloning restriction fragment for sequencing - (reply: 1)
  403. Cloning with real time PCR fragments - using SyberGreen from Applied Biosystem (reply: 3)
  404. Many colonies but no positive clone - (reply: 1)
  405. converting overhang into blunt end - can i use Pfu instead Klenow? (reply: 3)
  406. Site directed mutagenesis - wild type clone (reply: 4)
  407. Cloning - intronic sequences needed? - (reply: 4)
  408. sticky-end cloning problem? - (reply: 10)
  409. stick end cloning problem? - (reply: 1)
  410. How to clone enhancer sequence - (reply: 1)
  411. hurry! how can I clone large DNA fragment - noboday answer? (reply: 1)
  412. No Colonies on subcloning - (reply: 11)
  413. Taq/PFU polymerase A overhang - bad for blunt ending? (reply: 3)
  414. Degradation of PCR product during purfication - Purification of PCR product for cloning (reply: 2)
  415. BAC clone amplification - (reply: 1)
  416. Need cloning program - (reply: 10)
  417. Non-directional cloning - (reply: 5)
  418. problems in subcloning PCR products for TA cloning - (reply: 4)
  419. cloning membrane protein with 2 tags - (reply: 2)
  420. need help for Topo cloning - (reply: 6)
  421. No success with PCR cloning of a gene into expression vector - (reply: 5)
  422. problem cloning 2.5 kb fragment - (reply: 2)
  423. ligation with sticky and blunt ends - (reply: 3)
  424. directional cloning - (reply: 1)
  425. Insertions/deletions at cloning restriction site - (reply: 1)
  426. cloning a fragment using RE sites - protocol given really fuzzy. (reply: 4)
  427. long insert DNA TA clone problem,help me ! urgent! - (reply: 2)
  428. HELP! cloning cDNA from double-stranded cDNA synthesis - (reply: 2)
  429. TA cloning: if PCR product is not freshly prepared - (reply: 2)
  430. Directional cloning problem - (reply: 2)
  431. Blunting with klenow fragment - (reply: 2)
  432. Once more... cloning of a 13 kb fragment into pBR322 - (reply: 2)
  433. Molecular Cloning: UV Damage - (reply: 4)
  434. cloning the promoter from genomic dna - (reply: 9)
  435. single clone isolate showed multiple bands in DGGE - (reply: 1)
  436. What'sa phagemid? - phagemid cloning (reply: 3)
  437. Cloning Problem - (reply: 5)
  438. Cloning gene behind GFP - Vector? (reply: 2)
  439. Cloning and Charecterization of a Novel Receptor - (reply: 1)
  440. siRNA stable clone problem - protein level not reduced, but did have impact on cell function! (reply: 2)
  441. cloning from e.coli in e.coli - (reply: 4)
  442. Getting the right sequence by cloning - (reply: 7)
  443. Interpreting RE digest results after blunt cloning - (reply: 1)
  444. cloning/amplifying attenuate RNA transcripts - (reply: 6)
  445. PCR cloning problem - No colonies - (reply: 10)
  446. blunting with no success! - blunt end ligation (reply: 1)
  447. TOPO TA cloning - (reply: 2)
  448. Huge problem in cloning, need help quite urgent - (reply: 2)
  449. Blunt end cloning options - (reply: 1)
  450. topo XL cloning - (reply: 5)
  451. blunt end ligation - Help (reply: 11)
  452. tricky digestion for cloning gurus - (reply: 13)
  453. Cloning primer-dimer fragments - (reply: 2)
  454. Xba I and subcloning - (reply: 10)
  455. PCR or digestions for clone screen? - (reply: 5)
  456. KpnI Ligation Mystery - Bidirectional Cloning Working Unidirectionally (reply: 5)
  457. stange cloning results - pBluescript seeming to double up (reply: 6)
  458. what competent cells should I use to clone big size DNA - (reply: 8)
  459. Where to buy a full length clone? - (reply: 3)
  460. shorter PCR products after bisulfite treatment and cloning - (reply: 5)
  461. Blunt PCR cloning not working - (reply: 2)
  462. question for all you cloning gurus... - (reply: 8)
  463. Cloning a 2kb fragment! - (reply: 2)
  464. cloning 11 kb fragment - (reply: 2)
  465. cloning vector - cloning vector (reply: 1)
  466. which vector will be best for cloning and then expression - vector (reply: 1)
  467. Plz help me troubleshoot subcloning! - (reply: 6)
  468. Blunt ended ligation - (reply: 7)
  469. So frustrated... a question about blunt end cloning - (reply: 6)
  470. I feel desperate about cloning, who can help me? - (reply: 12)
  471. BamHI/XbaI cloning, bad combination? - (reply: 3)
  472. TA Cloning - (reply: 4)
  473. Has anyone used In-Fusion Cloning Kit? - (reply: 2)
  474. cloning of big insert(6.4Kb) in pUC18/19 - (reply: 2)
  475. Problems cloning into pET Kanamycin resistance vectors - (reply: 7)
  476. Cloning - how to avoid religation and no insert? - (reply: 13)
  477. degradation of cloned protein in E. coli - (reply: 2)
  478. deletion during cloning - (reply: 3)
  479. gene clone - (reply: 1)
  480. No insert for cloning PCR product into expression vector - (reply: 5)
  481. Ligation help ! - blunt end ligation probs. (reply: 4)
  482. TOPO cloning reaction transformation after 2 days - (reply: 1)
  483. cloning problems - help! (reply: 4)
  484. rabbit intestinal cDNA - Full length cloning (reply: 1)
  485. Cloning PCR product - double bands or heterozygote (reply: 5)
  486. cloning systems - pGEM-T easy vector system versus TOPO TA cloning? (reply: 4)
  487. blunt ended cloning problem - (reply: 7)
  488. How to purify and clone a 88 bp fragment? - (reply: 4)
  489. cloning of PCR product - (reply: 1)
  490. Strange things in clone - (reply: 6)
  491. cDNA cloning question - (reply: 7)
  492. Many Problems with Cloning - (reply: 2)
  493. Cloning using RT-PCR for long fragment of 6kb - (reply: 1)
  494. T4 blunting follow by digestion - (reply: 2)
  495. Allele specific cloning efficiency after bisulfite treatment - (reply: 4)
  496. yeast contamination - problem in cloning (reply: 4)
  497. help pcr cloning problem - help pcr cloning problem!!!!!1 (reply: 2)
  498. Cloning in old days - (reply: 2)
  499. Cloned genes - (reply: 1)
  500. FLAG Peptide how much to use w/M2 clone - competition w/M2 clone for immunocyto (reply: 1)
  501. topo cloning for sequencing? - (reply: 3)
  502. Problem with directional cloning - Problem with directional cloning (reply: 8)
  503. ACK! Blunt end cloning - I'm confused.. - (reply: 2)
  504. question about PCR clone a cDNA - (reply: 9)
  505. Strange cloning pattern - Vector seems to religate with incompatible ends (reply: 8)
  506. Whole gene cloning - (reply: 2)
  507. cDNA cloning problem - No product in PCR (reply: 5)
  508. Quick cloning question - Purification of PCR fragment (reply: 1)
  509. Directional cloning and clone mutations - each clone has a different mutation! (reply: 1)
  510. Cloning issue - vector insert ratio - (reply: 3)
  511. one end blunt end and other end sticky end-- ligation - vector with one end sticky and other blunt end (reply: 13)
  512. subcloning fragments - cloning (reply: 5)
  513. cloning large gene - advice (reply: 2)
  514. stupid redasoft visual cloning - (reply: 4)
  515. How to predict a promoter region for a gene - Promoter cloning + genes into vector (reply: 6)
  516. Validity of sequences via TA cloning - (reply: 1)
  517. TA cloning Vector - ...how to prepare it myself (reply: 1)
  518. cloning problems - cloning problems (reply: 3)
  519. Negative controls in cloning? - (reply: 4)
  520. Sticky ends to blunt - (reply: 5)
  521. quick cloning question - (reply: 3)
  522. problems cloning into plenti6/V5 lentiviral vector - (reply: 2)
  523. TOPO TA Vector Cloning problem - no colonies - (reply: 5)
  524. Problem with subcloning - (reply: 3)
  525. help needed for large construct cloning - (reply: 6)
  526. Cloning difficulty - Problems subcloning a very large fragment... (reply: 6)
  527. trouble with TOPO TA CLONING KIT for SEQUENCING - (reply: 8)
  528. Two cloning and expression problems - (reply: 2)
  529. cloning shRNA - help (reply: 3)
  530. cloning problem - sticky end cloning with PCR insert - (reply: 3)
  531. Experience with fusion cloning system? - (reply: 8)
  532. how do i analyse cloned sequences - Clone and DNA sequence (reply: 12)
  533. cloning question - sticky ends - has anyone tried this and would it work? (reply: 3)
  534. Another sub- cloning protocol not working - Mol. Biol. (reply: 1)
  535. Gateway Blunt end cloning - no colonies at all (reply: 1)
  536. TOPO XL cloning with 7.5kb PCR product - troubleshooting help (reply: 1)
  537. Cloning large pcr product - efficiency (reply: 2)
  538. One Step RT-PCR cloning problem - (reply: 2)
  539. cloning with rare 8-cutters - (reply: 2)
  540. TA cloning problem - (reply: 5)
  541. Help a first time cloner with PCR! - First time cloning (reply: 5)
  542. subcloning - (reply: 7)
  543. Mysterious gain in vector size after cloning! - (reply: 1)
  544. Cloning and PCR Problems - (reply: 1)
  545. Problem with cloning - can't clone a 3.2kb fragment (reply: 5)
  546. overlap extension and clone gene into pET -15b - a question?? (reply: 3)
  547. Cloning of sequence with PCR added sites - BstBI and BclI (reply: 2)
  548. A tried and true cloning protocol is FAILING miserably! - Molecular biology (reply: 6)
  549. Confusion with cloning strategy - (reply: 3)
  550. T/A cloning of cDNA? - PGEM-T vs pCR 2.1 T/A TOPO? (reply: 3)
  551. Trouble cloning a 8.5 kb fragment into a pTRE vector - (reply: 1)
  552. Cloning a big insert - insert size (reply: 1)
  553. Rolling circle replicating plasmid and transformation - Gene cloning (reply: 2)
  554. subcloning into a single NotI site - (reply: 11)
  555. Cloning a gene without the sequence of the target species - (reply: 3)
  556. subcloning of PCR product, please help! - subcloning problems (reply: 12)
  557. cloning primer orientation - orientation and complement of primers in a cloning (reply: 3)
  558. phosphatase alkaline before cloning? - (reply: 3)
  559. cloning genes with incompatible restriction sites - Protein expression (reply: 2)
  560. How would you identify and clone quinine transporters for P. - (reply: 1)
  561. importance of directionality in cloning - (reply: 1)
  562. Problems cloning cDNA using Pfx - Problem (reply: 2)
  563. difficulty in cloning oligos into vector - cloning difficulties (reply: 1)
  564. Topo cloning no colonies - (reply: 3)
  565. cloning tricks - (reply: 2)
  566. Cloning RACE products - (reply: 1)
  567. Contamination in PCR cloning - (reply: 3)
  568. Blunt End Ligation !Which god to pray? - (reply: 2)
  569. Cloning a large PCR product - trouble cloning large DNA (reply: 3)
  570. double sized PCR product from cloned cDNA - (reply: 6)
  571. Is kozak sequence necessary for cloning? - (reply: 4)
  572. problem in cloning work - (reply: 7)
  573. TA cloning with ABgene's extensor HiFi polymerase - (reply: 1)
  574. Screening of TA cloning by T7/Sp6 PCR possible? - (reply: 2)
  575. Cloning from scratch, please help... - (reply: 3)
  576. difficulty about cloning 3.5kb PCR product - (reply: 1)
  577. EcoRI cloning - correct orientation - Preferential orientation? (reply: 3)
  578. CIPping after Klenow blunting - Is CIP necessary in Blunt end ligation? (reply: 2)
  579. TA cloning with short PCR products - use of TA cloning kit (reply: 4)
  580. Cloning, ligation, transformation with pET - Problem re-ligating with NotI (reply: 1)
  581. Cloning into pUAST - Help needed (reply: 4)
  582. Promoter Cloning - PCR amplification of a promoter and cloning (reply: 1)
  583. Problem with TA PCR cloning - no insert in white colonies - (reply: 12)
  584. cloning problems - (reply: 1)
  585. cDNA cloning by PCR - cloning (reply: 3)
  586. TOPO TA pCR 2.1 cloning - (reply: 1)
  587. Blunting both vector and insert - klenow and CIPping (reply: 2)
  588. Question about cloning a gene coding an enzyme - (reply: 1)
  589. Cloning rare splice variants - Molecular Biology (reply: 1)
  590. Expression cloning system for protease expression - (reply: 2)
  591. Cloning Large Fragments in a pool of DNA fragments - (reply: 1)
  592. Large insert cloning - (reply: 1)
  593. Which is better? To blunt my insert and vector or use linkers? - To insert the 3.5 kb insert cut with EcoRI and SalI in to a (reply: 1)
  594. How big DNA fragment can I clone to pUC19 plasmid? - (reply: 2)
  595. How important is cloning of full-length genes? - (reply: 1)
  596. mat agar dish and a cloning problem - (reply: 4)
  597. BAC cloning problems - (reply: 2)
  598. blunt end ligation problems - (reply: 6)
  599. TA cloning - (reply: 5)
  600. Cloning problem - Insert DNA self-ligation? - (reply: 11)
  601. Which vector for cloning big PCR fragments? - (reply: 3)
  602. Regarding smaI restriction enzyme - Blunt end cloning (reply: 5)
  603. PCR Ligation cloning - (reply: 2)
  604. Problem with cloning by dephospharylation - Problem in cloning (reply: 4)
  605. cloning in pQE30 - (reply: 3)
  606. cloning big fragments - (reply: 1)
  607. Cloning problem - Digestion, ligation and transformation (reply: 6)
  608. Cloning into shRNA vectors - (reply: 4)
  609. Colony PCR for clone screen - Colony PCR before sending sequence (reply: 4)
  610. Cloning into GFP vector - (reply: 2)
  611. cloning of long viral fragment I - (reply: 2)
  612. TOPO-TA Cloning PCR product - Is amplicilin selection engough? (reply: 4)
  613. Cloning kit recommendations? - advice sought (reply: 2)
  614. pET100 TOPO cloning kit sucks - (reply: 2)
  615. cloning - (reply: 2)
  616. why so many small inserts? - puzzles in cloning (reply: 7)
  617. where to buy sterile grease used for isolate cell clone? - (reply: 2)
  618. TA cloning question - (reply: 2)
  619. Cloning pCAEGFP into XL1 E. coli - I cannot get any colonies (reply: 2)
  620. Cloning Isoforms - (reply: 1)
  621. cloning problem - cloning problem in GFP vector (reply: 4)
  622. Problem cloning PCR product into double-cut vector - (reply: 3)
  623. TA cloning -- funny sized bands - (reply: 1)
  624. Clone blunt ended insulin gene into pbr322 - help (reply: 3)
  625. Subcloning into large plasmid - (reply: 3)
  626. TA cloning problems - (reply: 2)
  627. Cloning RACE products - Problem with sequencing ends (reply: 3)
  628. Primer design - PCR for cloning (reply: 6)
  629. gateway cloning problems - (reply: 2)
  630. 1-2kb PCR products TOPO cloning troubles - help (reply: 4)
  631. RT-PCR for cloning low expressed genes - RT-PCR for cloning low expressed genes (reply: 1)
  632. T- vector clone - (reply: 3)
  633. Subcloning into lentiviral vectors - Very little to no plasmid after miniprep (reply: 7)
  634. PCR Cloning - Please Help - Urgent - Cloning using SalI & BamHI site (reply: 1)
  635. Cloning 10 Kb Fragment - (reply: 1)
  636. TA cloning kit problem - No single colonies after transformation!!! (reply: 10)
  637. cloning PCR fragment - PCR fragment is not getting cloned in the vector (reply: 2)
  638. Simple Ligation/cloning questions - question on dNTPs and ligation protocol (reply: 1)
  639. "Blunting" - (reply: 6)
  640. Cloning a long PCR product - (reply: 2)
  641. Problem in PCR cloning - (reply: 7)
  642. cloning size is not correct - 2 bands on the PCR product (reply: 2)
  643. still more about cloning PCR products - (reply: 9)
  644. large fragment cloning - (reply: 2)
  645. Cloning long fragments - (reply: 18)
  646. plasmid draw - Need software for Vector cloning design (reply: 4)
  647. cloning from PCR - (reply: 25)
  648. PCR cloning - PCR cloning (reply: 2)
  649. cloning upper stream of a gene - (reply: 1)
  650. help - stable transfection clone pick up - (reply: 33)
  651. Cloning using ds Oligos - concentration, phosphorylate? (reply: 2)
  652. Cloning Problems - #@%$&*^) (reply: 5)
  653. any experience in cloning a 3.5kb full length cDNA - (reply: 4)
  654. Help me - gene subclone (reply: 3)
  655. clone - how to clone an unknown gene (reply: 1)
  656. PCR Cloning - (reply: 1)
  657. Subcloning transgene from a BAC clone - Subcloning transgene from a BAC clone (reply: 1)
  658. Problem with cloning unstable HIV insert - (reply: 1)
  659. I need a little help with TA cloning - TA cloning (reply: 1)
  660. cloning of lacZ - i can't clone the lac Z!! (reply: 1)
  661. Cloning of high-GC% gene into vector - (reply: 3)
  662. why the clone ware so small? - plamid transformation (reply: 1)
  663. Selection of StableTransfectants Expressing a High Density of Cloned M - (reply: 1)
  664. Cloning antibodies - (reply: 1)
  665. Any experience in improving blunt end ligation? - (reply: 6)
  666. subcloning - (reply: 5)
  667. Subcloning problem - pCR4-Topo -> pcDNA3 (reply: 1)
  668. Blunting 3' ends with T4 polymerase - (reply: 1)
  669. oligo cloning - (reply: 2)
  670. How can I clone the promoter region of my gene - (reply: 3)
  671. Help please!!! - Delection after cloning (reply: 1)
  672. Subcloning - (reply: 2)
  673. cloning question - (reply: 2)
  674. cloning 60nt Oligos - Help (reply: 1)
  675. ligation problems - cloning a PCR fragment - into a double-cut vector (reply: 4)
  676. clone Src and Ras - (reply: 1)
  677. 7kb clone trouble - topo (reply: 1)
  678. Sticky Problem to a Blunt end - Cloning (reply: 1)
  679. cloning of pcr product - (reply: 2)
  680. topo TA cloning kit (invitrogen) - URGENT (reply: 5)
  681. How to clone large DNA fragment into small vector! - (reply: 1)
  682. Deletion after cloning - (reply: 2)
  683. Cloning error prone PCR products - (reply: 1)
  684. How can I clone the upstream or downstraem of my gene?? - How can I clone the upstream or downstraem of my gene?? (reply: 1)
  685. Re: PCR cloning problem - (reply: 1)
  686. why the clone grow so slowly - (reply: 1)