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cloning large gene - advice (Aug/26/2005 )

i would like to clone a gene that is over 11kb to be used in an expression vector. does anyone have any suggestions on how to go about cloning such a large gene?

any help would be much appreciated.


Use a low copy number vector.


DNA sequencing software

-Daniel Tillett-

Or even better, use an inducible copy number vector such as pSCANS, which is normally low copy number, but can be induced (IPTG) to high copy number for plasmid prep.