cloning large gene - advice (Aug/26/2005 )
i would like to clone a gene that is over 11kb to be used in an expression vector. does anyone have any suggestions on how to go about cloning such a large gene?
any help would be much appreciated.
Use a low copy number vector.
DNA sequencing software
Or even better, use an inducible copy number vector such as pSCANS, which is normally low copy number, but can be induced (IPTG) to high copy number for plasmid prep.