siRNA Cloning method - (May/01/2006 )
Hi, I am working on spreading of gene silencing in transgenic plants. My new project is to clone the small RNA fractions of the silenced plants. For this I am looking for one detail protocol to clone the small RNA/ siRNA. Can anybody provide me one detail protocol to clone siRNA?
you will find some protocols in following link
and more explain for it in a review paper
FEBS Lett. 2005 Oct 31;579(26):5830-40. Epub 2005 Aug 18.
Thanks rshi, I will study these protocols..............