TA Cloning - TA cloning (Aug/14/2008 )
How can i add 3' A overhangs to my PCR fragment without amplification.
-biolearner-
QUOTE (biolearner @ Aug 14 2008, 06:28 PM)
How can i add 3' A overhangs to my PCR fragment without amplification.
Incubate your PCR product with dATP and Taq at 72C for ~30min. Works great!
If you amplified with Taq from the begining your fragment will already have the overhangs.
-almost a doctor-
Hallo YOU CAn use terminal nucleotide transferase ant ATP to reach this, I suggest you want at clone your Pcr product
-baxapoptoaia-