cloning help about pBudCE4.1 - (Jul/25/2007 )
Hi everybody,
I have two proteins I want to clone into pBudCE4.1. It has two multiple cloning sites after CMV and EF-1α promoters. I want to know their transcription efficiency. Is there anyone using this vector?
Any help would be appreciated..
Burcu
-bsengez-
Which species is the CMV is derived?. Also its important to know which cells are you planning to test them. For eg. CMV doesn't work in neurons but otherwise is a very powerful promoter. murine CMV is a very powerful one. Some human CMV promoters are weak comparatively.
No idea about EF1-alpha promoter.
-scolix-