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Cloning in old days - (Sep/17/2005 )

Hi everybody;

I was wondering if anybody gives me some info about amplifying of genes before PCR was discovered. If there is anybody knowing this, I do appreciate if s/he gives me some hand. Thanks in advance.

Regards

Hak

-hmohamma-

QUOTE (hmohamma @ Sep 18 2005, 12:57 AM)
Hi everybody;

I was wondering if anybody gives me some info about amplifying of genes before PCR was discovered. If there is anybody knowing this, I do appreciate if s/he gives me some hand. Thanks in advance.

Regards

Hak

library screening, cloning into plasmids, amplifying in bacteria etc...

Can you imagine how life in a lab was before there were restriction enzymes available....

-Theo22-

Read an old copy of Maniatis' Molecular Cloning .

Daniel

DNA sequencing

-Daniel Tillett-