Protocol Online logo
Top : Forum Archives: : Molecular Cloning

cloning of big insert(6.4Kb) in pUC18/19 - (Nov/28/2005 )

Dear all
I am trying to clone 6.4 Kb insert in pUC19. But I am not able to do it. Please help me out.
Seema sad.gif


could you tell me what problem you have faced?


Dear all
Sometimes I am not getting colony after transformation. When I am getting colony it is not positive. Smaller size clone I am getting. and it is not releasing. After restriction digestion and purification my insert concentration is very less.
Help me with your experience.