blunt end - filling, polishing (Aug/01/2007 )
hi
if I cut with HIndIII
I'll generat a fragment like below
A/AGCT T
T TCGA/A
5'---A] AGCTT----3'
3'---TTCGA [A----5'
what is the best way to make it blunt.
filling polishing.
filling will give me that:correct?
5'---AAGCT] [AGCTT----3'
3'---TTCGA] [TCGAA----5'
and polishing will give me this: correct?
5'---A] [T----3'
3'---T] [A----5'
SO if this is correct what enzyme do that
thanks
-ulujm-
I always use Pfu polymerase to blunt sticky ends. In this case, this will fill in and give you the sequence you describe.
-dpo-