Subcloning problem - pCR4-Topo -> pcDNA3 (Jul/17/2002 )
I digested the vectors(pCR4-TOPO and pcDNA3 - from Invitrogen) with EcoRI and performed an in-gel ligation. Though after transforming DH5Alphas, next day i get up to 100 very very small colonies, which i am unable to grow in a selective medium. (control plate was negative). Can anyone help me?
If the DH5s are home made I'd pitch 'em and use another strain like XL-1 Blue or TOP 10. TOP 10 are made for use with TOPO.
(Edited by milanoj at 1:40 am on July 17, 2001)