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Cloning kit recommendations? - advice sought (Jan/05/2005 )

I'm writing to ask for opinions on cloning kits. I am trying to decide among kits from Invitrogen, Qiagen and a few other manufacturers. I would like to clone genomic DNA from Melanoplus grasshoppers in order to create a library that can be used to screen for SNP loci. I am targetting 1-2.5 Kb fragments of DNA. Thanks in advance for your feedback.


I guess you want TA cloning, right? I would recommend Invitrogen.


I totally agree with the above, TA cloning from Invitrogen works!