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cloning with pGEM T-easy vector - question about the vector. (Mar/07/2008 )

This sounds dumb and I think I know the answer but looking at the vector map has me confused. Before being able to use the pGEM T-easy vector does it need to be digested so it's linearized or is the insert site already open? Thanks!

-katjo-

Hey,

if you check the Promega website (pGEM-T easy Vector systems, FAQS), it says that the vector is linearized (EcoRI/NotI sites).

http://www.promega.com/catalog/catalogprod...roductleaf_1039

-zek-

QUOTE (zek @ Mar 7 2008, 01:04 PM)
Hey,

if you check the Promega website (pGEM-T easy Vector systems, FAQS), it says that the vector is linearized (EcoRI/NotI sites).

http://www.promega.com/catalog/catalogprod...roductleaf_1039


Awesome! I kept looking through the manual and everywhere I could think of on the site - I even checked the FAQs but just didn't come across that one. Thanks!

-katjo-