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TOPO cloning reaction transformation after 2 days - (Oct/23/2005 )


I have plated my transformed TOPO cloning reaction on the wrong agar plates (silly me smile.gif). Does anyone have experience with reusing of a TOPO reaction mix after two days in the fridge (4°C)? I still have a few microliters left of the 6ul reaction.
Will I get (insert containing) colonies or should I prepare a new mix?

Thanks in advance,




I would transform away -- I'd bet you'll be fine.