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Is this clone the correct one? - Restriction digestion doubt (Aug/19/2007 )

Hi everyone,
Can someone help me with the followng cloning problem:

I digested plasmid DNA with 2 restriction enzymes ApaI (GGGCC/C) and XbaI ( T/CTAGA)
after I used Klenow to fill in and ligated with T4 DNA ligase.

I expected to get as a results the following DNA sequence: TCTAGGGCCC
but instead I got the following : TCTAGC

Is the clone I got, correct?



yes, the clone you have is correct.

The ApaI restriction enzyme leave a 3' over hang. Klenow have a 3'-5' exonuclease activity. So rather then filling in a 3' overhang, it will remove said overhang, leaving a blunt end