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Plasmid Related Discussions
  1. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
  2. Doubt on HisTag protein purification miniprep - (reply: 3)
  3. Are relaxed plasmids origin of rep sensitive to bp substitution in RNA I/II sequ - (reply: 5)
  4. How stable is double digested plasmid - (reply: 7)
  5. low plasmid concentration - (reply: 1)
  6. What make a plasmid candidate for stable transfection - (reply: 1)
  7. Lower Plasmid Yields - (reply: 5)
  8. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  9. Question on plasmids - (reply: 4)
  10. *NO* DNA eluted from plasmid prep - Why? - (reply: 12)
  11. How much plasmid for transformation? - (reply: 8)
  12. HELP: From Plasmid Vector to Bacterial Chromosome - (reply: 2)
  13. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  14. plasmid repositories - (reply: 2)
  15. Why the insert become shorter after inserting into plasmid? - (reply: 2)
  16. real time qPCR standard range plasmid - (reply: 3)
  17. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  18. Low Yield Miniprep - (reply: 5)
  19. How to know the plasmid extracted are indeed the plasmid of interest? - (reply: 4)
  20. Plasmids recombining - (reply: 3)
  21. Plasmid Methylation - (reply: 3)
  22. Why are there two origin of replications in plasmids - (reply: 3)
  23. agarose electrophoresis gel has 500 bp bright smear after mini prep - (reply: 1)
  24. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  25. Plasmid Amplification Issue - (reply: 6)
  26. Why do we create own customised plasmid rather than buying iy? - (reply: 1)
  27. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  28. Can't isolate plasmid from cloned E.coli - (reply: 4)
  29. Denatured plasmid for pcr - (reply: 1)
  30. Using more transformed bacteria for midiprep - (reply: 2)
  31. Screen for F plasmid in e. coli - (reply: 1)
  32. Plasmid Linearization Problems - (reply: 9)
  33. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  34. plasmid construction - (reply: 2)
  35. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  36. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  37. ethanol precipitation plasmid DNA - (reply: 3)
  38. Plasmid storage conditions - (reply: 5)
  39. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  40. Temperature sensitivity of Plasmids - (reply: 1)
  41. New (free) plasmid mapping program - (reply: 4)
  42. removing part from plasmid - (reply: 18)
  43. Help with plasmid extraction and redissolving? - (reply: 5)
  44. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  45. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  46. Promoter sequence search in a plasmid map - (reply: 3)
  47. Heat vs pH during plasmid extraction - (reply: 8)
  48. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  49. Heating elution buffer for plasmid prep - (reply: 1)
  50. Using pBABE plasmids for regular transfection - (reply: 1)
  51. Rxn not working - amplify/linearize plasmid - (reply: 3)
  52. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  53. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  54. RNA contamination in plasmid extracted - (reply: 1)
  55. Problem in plasmid digestion after transformation - (reply: 4)
  56. Call for help! Plasmid extraction problem - (reply: 4)
  57. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  58. How can addgene sell plasmids with patented technology - (reply: 2)
  59. Transient transfection with large plasmid - (reply: 3)
  60. Midiprep from mini prep DNA - (reply: 2)
  61. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  62. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  63. Plasmids DNA on filter paper - (reply: 1)
  64. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  65. Storage life of plasmid DNA in water - (reply: 5)
  66. Plasmid DNA digestion problem - (reply: 3)
  67. free online DNA & plasmid editing tool - (reply: 1)
  68. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  69. Appearance of a salty plasmid preparation - (reply: 1)
  70. Transformation of Restricted plasmid - (reply: 3)
  71. Why is my insert missing from my plasmid? - (reply: 4)
  72. Plasmid digestion and gel purification using HindIII - (reply: 3)
  73. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  74. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  75. Colonies grown without plasmid - (reply: 7)
  76. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  77. Plasmid on blotting paper - (reply: 1)
  78. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  79. What plasmid sizes do you work with for SDM? - (reply: 1)
  80. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  81. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  82. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  83. Running undigested plasmids on gel - (reply: 2)
  84. Plasmid isolation from Enterococcus - (reply: 1)
  85. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  86. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  87. Whole plasmid amplification by PCR - (reply: 2)
  88. Sequencing of the cloned plasmid - (reply: 5)
  89. Plasmid DNA contamination - (reply: 5)
  90. Problems with plasmid DNA extraction - (reply: 3)
  91. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  92. Can I quantify restricted plasmid and oligos? - (reply: 3)
  93. conditionally active plasmids - (reply: 1)
  94. Qiagen mini prep cell fragment pellet - (reply: 2)
  95. Transient transfection of linearized plasmids - (reply: 3)
  96. Transfecting three plasmids into hES cells - (reply: 2)
  97. HUVEC plasmid transfection challenges - (reply: 2)
  98. Plasmid DNA - (reply: 3)
  99. Genomic DNA and plasmid DNA isolation - (reply: 3)
  100. faint band after plasmid extraction - (reply: 7)
  101. plasmid extraction troubleshooting,please help !! - (reply: 9)
  102. plasmid digestion - problem with enzymes? - (reply: 4)
  103. Plasmid stability in Ecoli during storage - (reply: 3)
  104. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  105. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  106. Plasmid isolation from gram positive bacteria - (reply: 2)
  107. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  108. problems with plasmid miniprep - (reply: 8)
  109. Determining the amount of plasmid transfected - (reply: 1)
  110. How to check for the presence of plasmid? - (reply: 3)
  111. PCR with Plasmid recovered from filter paper - (reply: 6)
  112. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  113. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  114. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  115. restricted PCR plasmid runs slower - (reply: 2)
  116. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
  117. Plasmid compatibly groups - (reply: 5)
  118. Plasmid isolation from agrobacterium - (reply: 1)
  119. Cutting my plasmid made it larger - (reply: 7)
  120. Recreate original plasmid by cutting out insert - (reply: 2)
  121. posting sequences of plasmid? - (reply: 3)
  122. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  123. problem with plasmid digestion - (reply: 5)
  124. unexpected band in PCR with plasmid - (reply: 4)
  125. Sequencing Primers and Plasmids - (reply: 3)
  126. Replication of the Plasmid DNA - (reply: 6)
  127. expression plasmids for tRNA - (reply: 6)
  128. cloning pcr insert into plasmid - (reply: 2)
  129. Re-culturing empty plasmid - (reply: 1)
  130. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
  131. Plasmid Miniprep - (reply: 2)
  132. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  133. Plasmid DNA at RT for a few days - (reply: 4)
  134. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  135. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  136. plasmid rearrangement or sabotage - (reply: 2)
  137. Linearising plasmid before transfection? - (reply: 2)
  138. Smear in plasmid sample - (reply: 9)
  139. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  140. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  141. cloning a gene in to a plasmid - (reply: 5)
  142. Characterizing an unknown plasmid - (reply: 6)
  143. Plasmid Purification and Digestion Problem - (reply: 2)
  144. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  145. QuikChange with Different plasmids - (reply: 1)
  146. Co-transfection with 2 plasmids - (reply: 10)
  147. Single Plasmid Gel Band - (reply: 1)
  148. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  149. Transformation with a big plasmid - (reply: 4)
  150. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  151. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  152. digested plasmid - (reply: 2)
  153. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  154. isolation of plasmid (midi and maxi prep) - (reply: 18)
  155. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  156. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  157. plasmid tetR and tet promoter - (reply: 2)
  158. Plasmid clone in BSP - (reply: 1)
  159. Swich selection in plasmid - (reply: 3)
  160. problems with midiprep - (reply: 3)
  161. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  162. Hypothetical Synthetic Plasmid - (reply: 9)
  163. How many fragments in plasmid DNA prep? - (reply: 1)
  164. Problem with plasmids transformation - (reply: 6)
  165. Calcium chloride plasmid transfection - (reply: 1)
  166. How Much Plasmid? - (reply: 6)
  167. Plasmid isolation - (reply: 1)
  168. plasmid;GI; ACESSION NUMBER - (reply: 2)
  169. virus transduction vs plasmid transfection - (reply: 4)
  170. Maxiprep of freeze/thawed pellet. - (reply: 1)
  171. Bizarre changes in plasmid band size - (reply: 3)
  172. Buffer composition for miniprep kit - (reply: 6)
  173. plasmid repository - (reply: 2)
  174. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  175. Falsely High Yields on Qiagen Miniprep? Assayed by Nanodrop - (reply: 3)
  176. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  177. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  178. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  179. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  180. Pls help me with this plasmid!!! - (reply: 1)
  181. Standard curve using plasmid - (reply: 1)
  182. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  183. Methods of plasmid transformation? only heat shock? - (reply: 1)
  184. No plasmid after a maxi prep mystery - (reply: 4)
  185. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  186. Co-expression of 2 plasmids in E.coli - (reply: 1)
  187. How to avoid plasmid instability - (reply: 4)
  188. Plasmid vs cDNA amplification by PCR - (reply: 2)
  189. Shear-induced damage of plasmid by vortexing - (reply: 1)
  190. RNase Band in old plasmid stored at -40 - (reply: 2)
  191. Plasmid vector for yeast and E. coli - (reply: 2)
  192. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  193. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  194. Plasmid digestion after transformation - (reply: 4)
  195. Puzzle about plamid and its digest, plz help - (reply: 8)
  196. Smear above plasmid dna - (reply: 3)
  197. result of plasmid extraction - (reply: 2)
  198. plasmid extraction - (reply: 5)
  199. Made the switch from Invitrogen Miniprep to Promega PureYield. What's going - (reply: 1)
  200. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  201. plasmid extraction - (reply: 3)
  202. No DNA after midiprep - (reply: 2)
  203. Is that a Plasmid??? - (reply: 5)
  204. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  205. TRADITIONAL METHOD FOR PLASMIDEXTRACTION - (reply: 1)
  206. plasmid extraction - (reply: 13)
  207. Cloning two genes in the same plasmid - (reply: 4)
  208. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  209. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  210. Plasmid, gene orientation, insert orientation. - (reply: 1)
  211. Plasmid recovery problem - (reply: 3)
  212. Electroporated plasmid loses the insert - (reply: 8)
  213. Plasmid sequencing without primers - (reply: 1)
  214. RE site on complimentary strand of plasmid? - (reply: 1)
  215. How much of plasmid should be cut to see the gene - (reply: 1)
  216. Plasmid sequencing - (reply: 1)
  217. Plasmid isolation Qiagen - (reply: 2)
  218. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  219. Amplify fragment in plasmid? - (reply: 4)
  220. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  221. failure in miniprep - (reply: 1)
  222. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  223. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  224. Template DNA (plasmid) concentration for PCR - (reply: 4)
  225. co-expression of 2 plasmids in E.coli - (reply: 3)
  226. How to choose the right plasmids for certain types of cell? - (reply: 1)
  227. Plasmid digestion create a bigger plasmid - (reply: 1)
  228. whole plasmid pcr? - (reply: 6)
  229. Looking for a GFP plasmid (non-amp) - (reply: 3)
  230. turbid DNA from maxiprep - (reply: 3)
  231. extract plasmids from transfected eukaryotic cells - (reply: 6)
  232. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  233. Plasmid compatible with pBAD24 - (reply: 1)
  234. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  235. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  236. Endofree Maxiprep tube volume - (reply: 6)
  237. Miniprep OD beyond 2.00 - Troubleshooting-help desperately needed - (reply: 10)
  238. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  239. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  240. Low Miniprep Yield - (reply: 1)
  241. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  242. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  243. Problem with maxiprep - (reply: 2)
  244. ~200kb Plasmid DNA purification is not working - (reply: 1)
  245. ligating a 6 His tag to plasmid - (reply: 2)
  246. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  247. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  248. Plasmid sequencing failed why? - (reply: 10)
  249. Mini prep of frozen bacteria cells - (reply: 2)
  250. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  251. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  252. SLIM mutagenesis of large plasmids - (reply: 8)
  253. why 12 h culture for plasmid prep? - (reply: 2)
  254. Big plasmids and Transfection - (reply: 3)
  255. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  256. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  257. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  258. pcr amplify an insert in plasmid? - (reply: 2)
  259. odd plasmids - (reply: 2)
  260. Lactobacillus plasmid - (reply: 1)
  261. Will transfected plasmids induce cytokine production? - (reply: 1)
  262. Question about plasmid midiprep - (reply: 1)
  263. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  264. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  265. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  266. Overexpression plasmid is not expressing protein - (reply: 13)
  267. Extra band on gel after miniprep - (reply: 6)
  268. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  269. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  270. PCR problem - basic PCR for plasmid amplification (reply: 2)
  271. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  272. Help Miniprep kit SIGMA - (reply: 2)
  273. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  274. Low yields of plasmids from miniprep - (reply: 1)
  275. A gene is not inserted into the Plasmid - (reply: 2)
  276. Plasmid Question - (reply: 4)
  277. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  278. Weird migration of double digested plasmid - (reply: 4)
  279. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  280. Help for co-transfection of proteins and plasmid - (reply: 1)
  281. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  282. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  283. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  284. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  285. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  286. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  287. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  288. Undigestable/unrestrictable Plasmid? - (reply: 1)
  289. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  290. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  291. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  292. problems encountered when working on a large plasmid - (reply: 2)
  293. make a Mega plasmid mutation - (reply: 1)
  294. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  295. Plasmid standard problem - (reply: 2)
  296. Do plasmids work in bacteria? - (reply: 1)
  297. CEN sequences on plasmids (yeast) - (reply: 4)
  298. Plasmid out of BL21 for mutagensis - (reply: 3)
  299. ORI names - name of a plasmid ori (reply: 11)
  300. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
  301. Change origin of a plasmid - (reply: 4)
  302. Better to sequence from plasmid? - (reply: 5)
  303. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  304. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  305. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  306. problem with pLK01 plasmid ligation - (reply: 2)
  307. Mini prep streaking - basic question (reply: 3)
  308. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  309. Maxiprep reagents - (reply: 5)
  310. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  311. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  312. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  313. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  314. PCR mutagenesis of plasmid - (reply: 5)
  315. Guanadine HCl for plasmid miniprep - (reply: 3)
  316. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  317. Not getting plasmid from miniprep - (reply: 7)
  318. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  319. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  320. Digesting large amount of plasmid - (reply: 4)
  321. Miniprep and sequencing - (reply: 4)
  322. Cloning <100 bp fragments into a plasmid - (reply: 7)
  323. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  324. Help! my plasmid size changed after ligation - (reply: 5)
  325. Electroporation doesn't like my plasmid??? - (reply: 4)
  326. Cloning in pCR2.1 plasmid - (reply: 7)
  327. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  328. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
  329. Miniprep DNA-strange nanodrop peak at 240-250nm? - (reply: 4)
  330. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  331. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  332. Cloning: Randomly recombined Plasmids? - (reply: 6)
  333. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  334. plasmid + sepharose - how do this work? (reply: 1)
  335. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  336. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  337. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  338. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  339. Plasmid size problems! - (reply: 3)
  340. Left DNA plasmid out for a week - (reply: 3)
  341. Transfecting primary cells with plasmid - (reply: 1)
  342. Extra bands after QIAGEN spin miniprep - (reply: 3)
  343. question about plasmid extraction - (reply: 2)
  344. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  345. Disappearing plasmid - (reply: 5)
  346. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  347. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  348. problem in plasmid transfection - (reply: 2)
  349. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  350. Plasmid DNA mini-prep - no good isolation (reply: 5)
  351. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  352. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  353. plasmid extraction - why dilute (reply: 3)
  354. very high molecular weight plasmid - (reply: 6)
  355. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  356. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  357. Concepts behind plasmid prep - (reply: 2)
  358. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  359. PCR off plasmid for screening - (reply: 2)
  360. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  361. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  362. Plasmid frameshift problem after clonning - (reply: 2)
  363. plasmid extraction kit wash buffer solution - (reply: 3)
  364. plasmid extraction kit solution composition - wash buffer (reply: 2)
  365. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  366. are plasmids used for lab work methylated - (reply: 1)
  367. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  368. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
  369. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  370. Isolation of protein crosslinked to plasmid - (reply: 3)
  371. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  372. Which Plasmid DNA for standard curve? - (reply: 5)
  373. Floating white pellet in miniprep - (reply: 2)
  374. Plasmid problem - From PCR product (reply: 7)
  375. Transfection - Plasmid Uptake Control - (reply: 2)
  376. Culture Grow well but No Plasmid - (reply: 2)
  377. software for plasmid drawing - (reply: 13)
  378. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  379. plasmid purification kit for transfection - (reply: 2)
  380. best plasmid purification kit for transfection - (reply: 1)
  381. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  382. Checking Plasmid Expression - (reply: 1)
  383. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  384. PCR a plasmid protein - (reply: 2)
  385. plasmid DNA disappear in the minipre - (reply: 2)
  386. Interpreting a Plasmid Profile gel photo - (reply: 3)
  387. marker swap plasmid - (reply: 4)
  388. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  389. plasmid dna isolation - (reply: 1)
  390. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  391. siRNA and plasmid cotransfection - (reply: 1)
  392. rna from plasmid - (reply: 1)
  393. Miniprep - (reply: 3)
  394. miniPrep troubleshooting - (reply: 7)
  395. Mini prep - (reply: 4)
  396. Plasmid curing method - (reply: 4)
  397. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  398. plasmid DNA lab question - (reply: 11)
  399. Problem amplifying plasmid - (reply: 1)
  400. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  401. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  402. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  403. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  404. Transport of miniprep - (reply: 2)
  405. role of glucose in plasmid isolation? - (reply: 1)
  406. sequencing clones after BSP : need miniprep ?? - (reply: 1)
  407. Plasmids for protein expression in mammalian cells - (reply: 2)
  408. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  409. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  410. plasmid, BAC, or cosmid? - (reply: 3)
  411. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  412. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  413. Mutation during transformation/maxiprep - (reply: 1)
  414. Best plasmid miniprep? - (reply: 13)
  415. Plasmid instability in E.coli BL21? - (reply: 3)
  416. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  417. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  418. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  419. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  420. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  421. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  422. Plasmid linearization by PCR - (reply: 4)
  423. plasmid sequence identification problem - (reply: 2)
  424. plasmid construct problem - (reply: 3)
  425. How to get plasmid maps from publications? - (reply: 5)
  426. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  427. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  428. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  429. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  430. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  431. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  432. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  433. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  434. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  435. Plasmid sequence - (reply: 1)
  436. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  437. plasmid isolation - (reply: 4)
  438. Plasmid supercoiling affecting PCR? - (reply: 2)
  439. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  440. strange extra bands on digest of miniprep? - (reply: 5)
  441. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  442. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  443. problem with plasmid extraction - (reply: 3)
  444. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  445. How to transfect two plasmids in a cell - (reply: 10)
  446. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  447. How to separate large size plasmids ~12kb - (reply: 8)
  448. low plasmid yield from midi preps - (reply: 4)
  449. Plasmid isolation, help with dilution step? - (reply: 7)
  450. full digestion of plasmid to get only dNTPs - (reply: 3)
  451. size limit of plasmid origins - (reply: 2)
  452. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  453. confusion with different forms of plasmid - (reply: 3)
  454. Plasmid as standard - Plasmid calculation (reply: 6)
  455. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  456. Effect of EtBr on plasmid supercoiling - (reply: 5)
  457. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  458. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  459. Digestion of a plasmid - (reply: 1)
  460. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  461. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  462. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  463. Amplifying plasmid - non specific binding of primer - (reply: 3)
  464. Storing plasmid at 4 degrees C - (reply: 5)
  465. Program to draw plasmids - draw plasmids (reply: 9)
  466. CIP required for double digestion of plasmid? - (reply: 3)
  467. Plasmid replication - Plasmid replication (reply: 13)
  468. Help with cloning a plasmid that contains repeats! - (reply: 2)
  469. Plasmid extraction sans commercial kits - (reply: 3)
  470. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  471. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  472. mutations in plasmids - (reply: 3)
  473. Sequential restriction digestion of plasmid - (reply: 58)
  474. miniprep and maxiprep - (reply: 9)
  475. plasmid spin column usage - (reply: 5)
  476. Stored bacteria lost plasmids? - (reply: 12)
  477. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  478. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  479. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  480. PLASMID ISOLATION - (reply: 10)
  481. Frustrated with Plasmid Preps - need help! - (reply: 14)
  482. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)