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Plasmid Related Discussions
  1. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  2. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  3. Pls help me with this plasmid!!! - (reply: 1)
  4. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  5. Methods of plasmid transformation? only heat shock? - (reply: 1)
  6. No plasmid after a maxi prep mystery - (reply: 4)
  7. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  8. Co-expression of 2 plasmids in E.coli - (reply: 1)
  9. How to avoid plasmid instability - (reply: 4)
  10. Plasmid vs cDNA amplification by PCR - (reply: 2)
  11. Shear-induced damage of plasmid by vortexing - (reply: 1)
  12. RNase Band in old plasmid stored at -40 - (reply: 2)
  13. Plasmid vector for yeast and E. coli - (reply: 2)
  14. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  15. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  16. Plasmid digestion after transformation - (reply: 3)
  17. Puzzle about plamid and its digest, plz help - (reply: 8)
  18. Smear above plasmid dna - (reply: 3)
  19. result of plasmid extraction - (reply: 2)
  20. plasmid extraction - (reply: 5)
  21. Made the switch from Invitrogen Miniprep to Promega PureYield. What's going - (reply: 1)
  22. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  23. plasmid extraction - (reply: 3)
  24. No DNA after midiprep - (reply: 2)
  25. Is that a Plasmid??? - (reply: 5)
  26. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  27. TRADITIONAL METHOD FOR PLASMIDEXTRACTION - (reply: 1)
  28. plasmid extraction - (reply: 13)
  29. Cloning two genes in the same plasmid - (reply: 4)
  30. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  31. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  32. Plasmid, gene orientation, insert orientation. - (reply: 1)
  33. Plasmid recovery problem - (reply: 3)
  34. Electroporated plasmid loses the insert - (reply: 8)
  35. Plasmid sequencing without primers - (reply: 1)
  36. RE site on complimentary strand of plasmid? - (reply: 1)
  37. How much of plasmid should be cut to see the gene - (reply: 1)
  38. Plasmid sequencing - (reply: 1)
  39. Plasmid isolation Qiagen - (reply: 2)
  40. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  41. Amplify fragment in plasmid? - (reply: 4)
  42. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  43. failure in miniprep - (reply: 1)
  44. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  45. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  46. Template DNA (plasmid) concentration for PCR - (reply: 4)
  47. co-expression of 2 plasmids in E.coli - (reply: 3)
  48. How to choose the right plasmids for certain types of cell? - (reply: 1)
  49. Plasmid digestion create a bigger plasmid - (reply: 1)
  50. whole plasmid pcr? - (reply: 6)
  51. Looking for a GFP plasmid (non-amp) - (reply: 4)
  52. turbid DNA from maxiprep - (reply: 3)
  53. extract plasmids from transfected eukaryotic cells - (reply: 6)
  54. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  55. Plasmid compatible with pBAD24 - (reply: 1)
  56. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  57. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  58. Endofree Maxiprep tube volume - (reply: 6)
  59. Miniprep OD beyond 2.00 - Troubleshooting-help desperately needed - (reply: 10)
  60. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  61. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  62. Low Miniprep Yield - (reply: 1)
  63. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  64. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  65. Problem with maxiprep - (reply: 2)
  66. ~200kb Plasmid DNA purification is not working - (reply: 1)
  67. ligating a 6 His tag to plasmid - (reply: 2)
  68. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  69. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  70. Plasmid sequencing failed why? - (reply: 10)
  71. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  72. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  73. SLIM mutagenesis of large plasmids - (reply: 8)
  74. why 12 h culture for plasmid prep? - (reply: 2)
  75. Big plasmids and Transfection - (reply: 3)
  76. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  77. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  78. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  79. pcr amplify an insert in plasmid? - (reply: 2)
  80. odd plasmids - (reply: 2)
  81. Lactobacillus plasmid - (reply: 1)
  82. Will transfected plasmids induce cytokine production? - (reply: 1)
  83. Question about plasmid midiprep - (reply: 1)
  84. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  85. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  86. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  87. Overexpression plasmid is not expressing protein - (reply: 11)
  88. Extra band on gel after miniprep - (reply: 6)
  89. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  90. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  91. PCR problem - basic PCR for plasmid amplification (reply: 2)
  92. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  93. Help Miniprep kit SIGMA - (reply: 2)
  94. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  95. Low yields of plasmids from miniprep - (reply: 1)
  96. A gene is not inserted into the Plasmid - (reply: 2)
  97. Plasmid Question - (reply: 4)
  98. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  99. Weird migration of double digested plasmid - (reply: 4)
  100. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  101. Help for co-transfection of proteins and plasmid - (reply: 1)
  102. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  103. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  104. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  105. Really strange problem with sequencing and plasmid purification! - (reply: 7)
  106. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  107. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  108. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  109. Undigestable/unrestrictable Plasmid? - (reply: 1)
  110. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  111. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  112. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  113. problems encountered when working on a large plasmid - (reply: 2)
  114. make a Mega plasmid mutation - (reply: 1)
  115. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  116. Plasmid standard problem - (reply: 2)
  117. Do plasmids work in bacteria? - (reply: 1)
  118. CEN sequences on plasmids (yeast) - (reply: 4)
  119. Plasmid out of BL21 for mutagensis - (reply: 3)
  120. ORI names - name of a plasmid ori (reply: 11)
  121. Change origin of a plasmid - (reply: 4)
  122. Better to sequence from plasmid? - (reply: 5)
  123. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  124. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  125. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  126. problem with pLK01 plasmid ligation - (reply: 2)
  127. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  128. Maxiprep reagents - (reply: 5)
  129. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  130. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  131. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  132. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  133. PCR mutagenesis of plasmid - (reply: 5)
  134. Guanadine HCl for plasmid miniprep - (reply: 3)
  135. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  136. Not getting plasmid from miniprep - (reply: 7)
  137. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  138. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  139. Digesting large amount of plasmid - (reply: 4)
  140. Miniprep and sequencing - (reply: 4)
  141. Cloning <100 bp fragments into a plasmid - (reply: 7)
  142. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  143. Help! my plasmid size changed after ligation - (reply: 5)
  144. Electroporation doesn't like my plasmid??? - (reply: 4)
  145. Cloning in pCR2.1 plasmid - (reply: 7)
  146. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  147. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
  148. Miniprep DNA-strange nanodrop peak at 240-250nm? - (reply: 4)
  149. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  150. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  151. Cloning: Randomly recombined Plasmids? - (reply: 6)
  152. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  153. plasmid + sepharose - how do this work? (reply: 1)
  154. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  155. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  156. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  157. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  158. Plasmid size problems! - (reply: 3)
  159. Left DNA plasmid out for a week - (reply: 3)
  160. Transfecting primary cells with plasmid - (reply: 1)
  161. Extra bands after QIAGEN spin miniprep - (reply: 3)
  162. question about plasmid extraction - (reply: 2)
  163. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  164. Disappearing plasmid - (reply: 5)
  165. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  166. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  167. problem in plasmid transfection - (reply: 2)
  168. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  169. Plasmid DNA mini-prep - no good isolation (reply: 5)
  170. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  171. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  172. plasmid extraction - why dilute (reply: 3)
  173. very high molecular weight plasmid - (reply: 6)
  174. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  175. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  176. Concepts behind plasmid prep - (reply: 2)
  177. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  178. PCR off plasmid for screening - (reply: 2)
  179. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  180. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  181. Plasmid frameshift problem after clonning - (reply: 2)
  182. plasmid extraction kit wash buffer solution - (reply: 3)
  183. plasmid extraction kit solution composition - wash buffer (reply: 2)
  184. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  185. are plasmids used for lab work methylated - (reply: 1)
  186. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  187. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
  188. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  189. Isolation of protein crosslinked to plasmid - (reply: 3)
  190. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  191. Which Plasmid DNA for standard curve? - (reply: 5)
  192. Floating white pellet in miniprep - (reply: 2)
  193. Plasmid problem - From PCR product (reply: 7)
  194. Transfection - Plasmid Uptake Control - (reply: 2)
  195. Culture Grow well but No Plasmid - (reply: 2)
  196. software for plasmid drawing - (reply: 7)
  197. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  198. plasmid purification kit for transfection - (reply: 2)
  199. best plasmid purification kit for transfection - (reply: 1)
  200. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  201. Checking Plasmid Expression - (reply: 1)
  202. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  203. PCR a plasmid protein - (reply: 2)
  204. plasmid DNA disappear in the minipre - (reply: 2)
  205. Interpreting a Plasmid Profile gel photo - (reply: 3)
  206. marker swap plasmid - (reply: 4)
  207. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  208. plasmid dna isolation - (reply: 1)
  209. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  210. siRNA and plasmid cotransfection - (reply: 1)
  211. rna from plasmid - (reply: 1)
  212. Miniprep - (reply: 3)
  213. miniPrep troubleshooting - (reply: 7)
  214. Plasmid curing method - (reply: 4)
  215. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  216. plasmid DNA lab question - (reply: 11)
  217. Problem amplifying plasmid - (reply: 1)
  218. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  219. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  220. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  221. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  222. Transport of miniprep - (reply: 2)
  223. role of glucose in plasmid isolation? - (reply: 1)
  224. sequencing clones after BSP : need miniprep ?? - (reply: 1)
  225. Plasmids for protein expression in mammalian cells - (reply: 2)
  226. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  227. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  228. plasmid, BAC, or cosmid? - (reply: 3)
  229. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  230. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  231. Mutation during transformation/maxiprep - (reply: 1)
  232. Best plasmid miniprep? - (reply: 13)
  233. Plasmid instability in E.coli BL21? - (reply: 3)
  234. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  235. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  236. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  237. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  238. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  239. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  240. Plasmid linearization by PCR - (reply: 4)
  241. plasmid sequence identification problem - (reply: 2)
  242. plasmid construct problem - (reply: 3)
  243. How to get plasmid maps from publications? - (reply: 5)
  244. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  245. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  246. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  247. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  248. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  249. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  250. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  251. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  252. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  253. Plasmid sequence - (reply: 1)
  254. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  255. plasmid isolation - (reply: 4)
  256. Plasmid supercoiling affecting PCR? - (reply: 2)
  257. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  258. strange extra bands on digest of miniprep? - (reply: 5)
  259. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  260. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  261. problem with plasmid extraction - (reply: 2)
  262. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  263. How to transfect two plasmids in a cell - (reply: 10)
  264. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  265. How to separate large size plasmids ~12kb - (reply: 8)
  266. low plasmid yield from midi preps - (reply: 4)
  267. Plasmid isolation, help with dilution step? - (reply: 7)
  268. full digestion of plasmid to get only dNTPs - (reply: 3)
  269. size limit of plasmid origins - (reply: 2)
  270. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  271. confusion with different forms of plasmid - (reply: 3)
  272. Plasmid as standard - Plasmid calculation (reply: 6)
  273. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  274. Effect of EtBr on plasmid supercoiling - (reply: 5)
  275. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  276. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  277. Digestion of a plasmid - (reply: 1)
  278. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  279. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  280. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  281. Amplifying plasmid - non specific binding of primer - (reply: 3)
  282. Storing plasmid at 4 degrees C - (reply: 5)
  283. Program to draw plasmids - draw plasmids (reply: 6)
  284. CIP required for double digestion of plasmid? - (reply: 3)
  285. Plasmid replication - Plasmid replication (reply: 13)
  286. Help with cloning a plasmid that contains repeats! - (reply: 2)
  287. Plasmid extraction sans commercial kits - (reply: 3)
  288. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  289. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  290. mutations in plasmids - (reply: 3)
  291. Sequential restriction digestion of plasmid - (reply: 54)
  292. miniprep and maxiprep - (reply: 9)
  293. plasmid spin column usage - (reply: 5)
  294. Stored bacteria lost plasmids? - (reply: 12)
  295. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  296. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  297. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  298. PLASMID ISOLATION - (reply: 10)
  299. Frustrated with Plasmid Preps - need help! - (reply: 14)
  300. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)