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Plasmid Related Discussions
  1. a half less of plasmid copy number in the solution resulting from ddPCR than qPC - (reply: 1)
  2. Plasmid purification for Gibson Assembly - (reply: 2)
  3. Need to run gel after miniprep before digest? - (reply: 1)
  4. Co-transfection of multiple plasmids - (reply: 1)
  5. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
  6. Doubt on HisTag protein purification miniprep - (reply: 3)
  7. Are relaxed plasmids origin of rep sensitive to bp substitution in RNA I/II sequ - (reply: 5)
  8. How stable is double digested plasmid - (reply: 7)
  9. low plasmid concentration - (reply: 1)
  10. What make a plasmid candidate for stable transfection - (reply: 1)
  11. Lower Plasmid Yields - (reply: 5)
  12. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  13. Question on plasmids - (reply: 4)
  14. *NO* DNA eluted from plasmid prep - Why? - (reply: 12)
  15. How much plasmid for transformation? - (reply: 8)
  16. HELP: From Plasmid Vector to Bacterial Chromosome - (reply: 2)
  17. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  18. plasmid repositories - (reply: 2)
  19. Why the insert become shorter after inserting into plasmid? - (reply: 2)
  20. real time qPCR standard range plasmid - (reply: 3)
  21. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  22. Low Yield Miniprep - (reply: 5)
  23. How to know the plasmid extracted are indeed the plasmid of interest? - (reply: 4)
  24. Plasmids recombining - (reply: 3)
  25. Plasmid Methylation - (reply: 3)
  26. Why are there two origin of replications in plasmids - (reply: 3)
  27. agarose electrophoresis gel has 500 bp bright smear after mini prep - (reply: 1)
  28. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  29. Plasmid Amplification Issue - (reply: 6)
  30. Why do we create own customised plasmid rather than buying iy? - (reply: 1)
  31. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  32. Can't isolate plasmid from cloned E.coli - (reply: 4)
  33. Denatured plasmid for pcr - (reply: 1)
  34. Using more transformed bacteria for midiprep - (reply: 2)
  35. Screen for F plasmid in e. coli - (reply: 1)
  36. Plasmid Linearization Problems - (reply: 9)
  37. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  38. plasmid construction - (reply: 2)
  39. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  40. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  41. ethanol precipitation plasmid DNA - (reply: 3)
  42. Plasmid storage conditions - (reply: 5)
  43. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  44. Temperature sensitivity of Plasmids - (reply: 1)
  45. New (free) plasmid mapping program - (reply: 4)
  46. removing part from plasmid - (reply: 18)
  47. Help with plasmid extraction and redissolving? - (reply: 5)
  48. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  49. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  50. Promoter sequence search in a plasmid map - (reply: 3)
  51. Heat vs pH during plasmid extraction - (reply: 8)
  52. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  53. Heating elution buffer for plasmid prep - (reply: 1)
  54. Using pBABE plasmids for regular transfection - (reply: 1)
  55. Rxn not working - amplify/linearize plasmid - (reply: 3)
  56. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  57. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  58. RNA contamination in plasmid extracted - (reply: 1)
  59. Problem in plasmid digestion after transformation - (reply: 4)
  60. Call for help! Plasmid extraction problem - (reply: 4)
  61. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  62. How can addgene sell plasmids with patented technology - (reply: 2)
  63. Transient transfection with large plasmid - (reply: 3)
  64. Midiprep from mini prep DNA - (reply: 2)
  65. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  66. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  67. Plasmids DNA on filter paper - (reply: 1)
  68. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  69. Storage life of plasmid DNA in water - (reply: 5)
  70. Plasmid DNA digestion problem - (reply: 3)
  71. free online DNA & plasmid editing tool - (reply: 1)
  72. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  73. Appearance of a salty plasmid preparation - (reply: 1)
  74. Transformation of Restricted plasmid - (reply: 3)
  75. Why is my insert missing from my plasmid? - (reply: 4)
  76. Plasmid digestion and gel purification using HindIII - (reply: 3)
  77. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  78. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  79. Colonies grown without plasmid - (reply: 7)
  80. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  81. Plasmid on blotting paper - (reply: 1)
  82. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  83. What plasmid sizes do you work with for SDM? - (reply: 1)
  84. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  85. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  86. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  87. Running undigested plasmids on gel - (reply: 2)
  88. Plasmid isolation from Enterococcus - (reply: 1)
  89. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  90. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  91. Whole plasmid amplification by PCR - (reply: 2)
  92. Sequencing of the cloned plasmid - (reply: 5)
  93. Plasmid DNA contamination - (reply: 5)
  94. Problems with plasmid DNA extraction - (reply: 3)
  95. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  96. Can I quantify restricted plasmid and oligos? - (reply: 3)
  97. conditionally active plasmids - (reply: 1)
  98. Qiagen mini prep cell fragment pellet - (reply: 2)
  99. Transient transfection of linearized plasmids - (reply: 3)
  100. Transfecting three plasmids into hES cells - (reply: 2)
  101. HUVEC plasmid transfection challenges - (reply: 2)
  102. Plasmid DNA - (reply: 3)
  103. Genomic DNA and plasmid DNA isolation - (reply: 3)
  104. faint band after plasmid extraction - (reply: 7)
  105. plasmid extraction troubleshooting,please help !! - (reply: 9)
  106. plasmid digestion - problem with enzymes? - (reply: 4)
  107. Plasmid stability in Ecoli during storage - (reply: 3)
  108. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  109. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  110. Plasmid isolation from gram positive bacteria - (reply: 2)
  111. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  112. problems with plasmid miniprep - (reply: 8)
  113. Determining the amount of plasmid transfected - (reply: 1)
  114. How to check for the presence of plasmid? - (reply: 3)
  115. PCR with Plasmid recovered from filter paper - (reply: 6)
  116. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  117. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  118. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  119. restricted PCR plasmid runs slower - (reply: 2)
  120. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
  121. Plasmid compatibly groups - (reply: 5)
  122. Plasmid isolation from agrobacterium - (reply: 1)
  123. Cutting my plasmid made it larger - (reply: 7)
  124. Recreate original plasmid by cutting out insert - (reply: 2)
  125. posting sequences of plasmid? - (reply: 3)
  126. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  127. problem with plasmid digestion - (reply: 5)
  128. unexpected band in PCR with plasmid - (reply: 4)
  129. Sequencing Primers and Plasmids - (reply: 3)
  130. Replication of the Plasmid DNA - (reply: 6)
  131. expression plasmids for tRNA - (reply: 6)
  132. cloning pcr insert into plasmid - (reply: 2)
  133. Re-culturing empty plasmid - (reply: 1)
  134. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
  135. Plasmid Miniprep - (reply: 2)
  136. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  137. Plasmid DNA at RT for a few days - (reply: 4)
  138. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  139. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  140. plasmid rearrangement or sabotage - (reply: 2)
  141. Linearising plasmid before transfection? - (reply: 2)
  142. Smear in plasmid sample - (reply: 9)
  143. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  144. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  145. cloning a gene in to a plasmid - (reply: 5)
  146. Characterizing an unknown plasmid - (reply: 6)
  147. Plasmid Purification and Digestion Problem - (reply: 2)
  148. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  149. QuikChange with Different plasmids - (reply: 1)
  150. Co-transfection with 2 plasmids - (reply: 10)
  151. Single Plasmid Gel Band - (reply: 1)
  152. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  153. Transformation with a big plasmid - (reply: 4)
  154. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  155. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  156. digested plasmid - (reply: 2)
  157. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  158. isolation of plasmid (midi and maxi prep) - (reply: 18)
  159. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  160. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  161. plasmid tetR and tet promoter - (reply: 2)
  162. Plasmid clone in BSP - (reply: 1)
  163. Swich selection in plasmid - (reply: 3)
  164. problems with midiprep - (reply: 3)
  165. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  166. Hypothetical Synthetic Plasmid - (reply: 9)
  167. How many fragments in plasmid DNA prep? - (reply: 1)
  168. Problem with plasmids transformation - (reply: 6)
  169. Calcium chloride plasmid transfection - (reply: 1)
  170. How Much Plasmid? - (reply: 6)
  171. Plasmid isolation - (reply: 1)
  172. plasmid;GI; ACESSION NUMBER - (reply: 2)
  173. virus transduction vs plasmid transfection - (reply: 4)
  174. Maxiprep of freeze/thawed pellet. - (reply: 1)
  175. Bizarre changes in plasmid band size - (reply: 3)
  176. Buffer composition for miniprep kit - (reply: 6)
  177. plasmid repository - (reply: 2)
  178. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  179. Falsely High Yields on Qiagen Miniprep? Assayed by Nanodrop - (reply: 3)
  180. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  181. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  182. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  183. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  184. Pls help me with this plasmid!!! - (reply: 1)
  185. Standard curve using plasmid - (reply: 1)
  186. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  187. Methods of plasmid transformation? only heat shock? - (reply: 1)
  188. No plasmid after a maxi prep mystery - (reply: 4)
  189. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  190. Co-expression of 2 plasmids in E.coli - (reply: 1)
  191. How to avoid plasmid instability - (reply: 4)
  192. Plasmid vs cDNA amplification by PCR - (reply: 2)
  193. Shear-induced damage of plasmid by vortexing - (reply: 1)
  194. RNase Band in old plasmid stored at -40 - (reply: 2)
  195. Plasmid vector for yeast and E. coli - (reply: 2)
  196. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  197. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  198. Plasmid digestion after transformation - (reply: 4)
  199. Puzzle about plamid and its digest, plz help - (reply: 8)
  200. Smear above plasmid dna - (reply: 3)
  201. result of plasmid extraction - (reply: 2)
  202. plasmid extraction - (reply: 5)
  203. Made the switch from Invitrogen Miniprep to Promega PureYield. What's going - (reply: 1)
  204. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  205. plasmid extraction - (reply: 3)
  206. No DNA after midiprep - (reply: 2)
  207. Is that a Plasmid??? - (reply: 5)
  208. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  209. TRADITIONAL METHOD FOR PLASMIDEXTRACTION - (reply: 1)
  210. plasmid extraction - (reply: 13)
  211. Cloning two genes in the same plasmid - (reply: 4)
  212. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  213. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  214. Plasmid, gene orientation, insert orientation. - (reply: 1)
  215. Plasmid recovery problem - (reply: 3)
  216. Electroporated plasmid loses the insert - (reply: 8)
  217. Plasmid sequencing without primers - (reply: 1)
  218. RE site on complimentary strand of plasmid? - (reply: 1)
  219. How much of plasmid should be cut to see the gene - (reply: 1)
  220. Plasmid sequencing - (reply: 1)
  221. Plasmid isolation Qiagen - (reply: 2)
  222. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  223. Amplify fragment in plasmid? - (reply: 4)
  224. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  225. failure in miniprep - (reply: 1)
  226. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  227. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  228. Template DNA (plasmid) concentration for PCR - (reply: 4)
  229. co-expression of 2 plasmids in E.coli - (reply: 3)
  230. How to choose the right plasmids for certain types of cell? - (reply: 1)
  231. Plasmid digestion create a bigger plasmid - (reply: 1)
  232. whole plasmid pcr? - (reply: 6)
  233. Looking for a GFP plasmid (non-amp) - (reply: 3)
  234. turbid DNA from maxiprep - (reply: 3)
  235. extract plasmids from transfected eukaryotic cells - (reply: 6)
  236. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  237. Plasmid compatible with pBAD24 - (reply: 1)
  238. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  239. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  240. Endofree Maxiprep tube volume - (reply: 6)
  241. Miniprep OD beyond 2.00 - Troubleshooting-help desperately needed - (reply: 10)
  242. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  243. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  244. Low Miniprep Yield - (reply: 1)
  245. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  246. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  247. Problem with maxiprep - (reply: 2)
  248. ~200kb Plasmid DNA purification is not working - (reply: 1)
  249. ligating a 6 His tag to plasmid - (reply: 2)
  250. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  251. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  252. Plasmid sequencing failed why? - (reply: 10)
  253. Mini prep of frozen bacteria cells - (reply: 2)
  254. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  255. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  256. SLIM mutagenesis of large plasmids - (reply: 8)
  257. why 12 h culture for plasmid prep? - (reply: 2)
  258. Big plasmids and Transfection - (reply: 3)
  259. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  260. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  261. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  262. pcr amplify an insert in plasmid? - (reply: 2)
  263. odd plasmids - (reply: 2)
  264. Lactobacillus plasmid - (reply: 1)
  265. Will transfected plasmids induce cytokine production? - (reply: 1)
  266. Question about plasmid midiprep - (reply: 1)
  267. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  268. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  269. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  270. Overexpression plasmid is not expressing protein - (reply: 13)
  271. Extra band on gel after miniprep - (reply: 6)
  272. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  273. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  274. PCR problem - basic PCR for plasmid amplification (reply: 2)
  275. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  276. Help Miniprep kit SIGMA - (reply: 2)
  277. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  278. Low yields of plasmids from miniprep - (reply: 1)
  279. A gene is not inserted into the Plasmid - (reply: 2)
  280. Plasmid Question - (reply: 4)
  281. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  282. Weird migration of double digested plasmid - (reply: 4)
  283. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  284. Help for co-transfection of proteins and plasmid - (reply: 1)
  285. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  286. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  287. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  288. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  289. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  290. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  291. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  292. Undigestable/unrestrictable Plasmid? - (reply: 1)
  293. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  294. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  295. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  296. problems encountered when working on a large plasmid - (reply: 2)
  297. make a Mega plasmid mutation - (reply: 1)
  298. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  299. Plasmid standard problem - (reply: 2)
  300. Do plasmids work in bacteria? - (reply: 1)
  301. CEN sequences on plasmids (yeast) - (reply: 4)
  302. Plasmid out of BL21 for mutagensis - (reply: 3)
  303. ORI names - name of a plasmid ori (reply: 11)
  304. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
  305. Change origin of a plasmid - (reply: 4)
  306. Better to sequence from plasmid? - (reply: 5)
  307. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  308. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  309. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  310. problem with pLK01 plasmid ligation - (reply: 2)
  311. Mini prep streaking - basic question (reply: 3)
  312. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  313. Maxiprep reagents - (reply: 5)
  314. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  315. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  316. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  317. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  318. PCR mutagenesis of plasmid - (reply: 5)
  319. Guanadine HCl for plasmid miniprep - (reply: 3)
  320. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  321. Not getting plasmid from miniprep - (reply: 7)
  322. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  323. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  324. Digesting large amount of plasmid - (reply: 4)
  325. Miniprep and sequencing - (reply: 4)
  326. Cloning <100 bp fragments into a plasmid - (reply: 7)
  327. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  328. Help! my plasmid size changed after ligation - (reply: 5)
  329. Electroporation doesn't like my plasmid??? - (reply: 4)
  330. Cloning in pCR2.1 plasmid - (reply: 7)
  331. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  332. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
  333. Miniprep DNA-strange nanodrop peak at 240-250nm? - (reply: 4)
  334. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  335. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  336. Cloning: Randomly recombined Plasmids? - (reply: 6)
  337. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  338. plasmid + sepharose - how do this work? (reply: 1)
  339. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  340. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  341. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  342. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  343. Plasmid size problems! - (reply: 3)
  344. Left DNA plasmid out for a week - (reply: 3)
  345. Transfecting primary cells with plasmid - (reply: 1)
  346. Extra bands after QIAGEN spin miniprep - (reply: 3)
  347. question about plasmid extraction - (reply: 2)
  348. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  349. Disappearing plasmid - (reply: 5)
  350. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  351. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  352. problem in plasmid transfection - (reply: 2)
  353. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  354. Plasmid DNA mini-prep - no good isolation (reply: 5)
  355. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  356. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  357. plasmid extraction - why dilute (reply: 3)
  358. very high molecular weight plasmid - (reply: 6)
  359. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  360. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  361. Concepts behind plasmid prep - (reply: 2)
  362. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  363. PCR off plasmid for screening - (reply: 2)
  364. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  365. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  366. Plasmid frameshift problem after clonning - (reply: 2)
  367. plasmid extraction kit wash buffer solution - (reply: 3)
  368. plasmid extraction kit solution composition - wash buffer (reply: 2)
  369. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  370. are plasmids used for lab work methylated - (reply: 1)
  371. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  372. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
  373. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  374. Isolation of protein crosslinked to plasmid - (reply: 3)
  375. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  376. Which Plasmid DNA for standard curve? - (reply: 5)
  377. Floating white pellet in miniprep - (reply: 2)
  378. Plasmid problem - From PCR product (reply: 7)
  379. Transfection - Plasmid Uptake Control - (reply: 2)
  380. Culture Grow well but No Plasmid - (reply: 2)
  381. software for plasmid drawing - (reply: 13)
  382. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  383. plasmid purification kit for transfection - (reply: 2)
  384. best plasmid purification kit for transfection - (reply: 1)
  385. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  386. Checking Plasmid Expression - (reply: 1)
  387. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  388. PCR a plasmid protein - (reply: 2)
  389. plasmid DNA disappear in the minipre - (reply: 2)
  390. Interpreting a Plasmid Profile gel photo - (reply: 3)
  391. marker swap plasmid - (reply: 4)
  392. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  393. plasmid dna isolation - (reply: 1)
  394. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  395. siRNA and plasmid cotransfection - (reply: 1)
  396. rna from plasmid - (reply: 1)
  397. Miniprep - (reply: 3)
  398. miniPrep troubleshooting - (reply: 7)
  399. Mini prep - (reply: 4)
  400. Plasmid curing method - (reply: 4)
  401. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  402. plasmid DNA lab question - (reply: 11)
  403. Problem amplifying plasmid - (reply: 1)
  404. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  405. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  406. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  407. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  408. Transport of miniprep - (reply: 2)
  409. role of glucose in plasmid isolation? - (reply: 1)
  410. sequencing clones after BSP : need miniprep ?? - (reply: 1)
  411. Plasmids for protein expression in mammalian cells - (reply: 2)
  412. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  413. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  414. plasmid, BAC, or cosmid? - (reply: 3)
  415. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  416. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  417. Mutation during transformation/maxiprep - (reply: 1)
  418. Best plasmid miniprep? - (reply: 13)
  419. Plasmid instability in E.coli BL21? - (reply: 3)
  420. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  421. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  422. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  423. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  424. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  425. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  426. Plasmid linearization by PCR - (reply: 4)
  427. plasmid sequence identification problem - (reply: 2)
  428. plasmid construct problem - (reply: 3)
  429. How to get plasmid maps from publications? - (reply: 5)
  430. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  431. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  432. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  433. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  434. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  435. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  436. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  437. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  438. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  439. Plasmid sequence - (reply: 1)
  440. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  441. plasmid isolation - (reply: 4)
  442. Plasmid supercoiling affecting PCR? - (reply: 2)
  443. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  444. strange extra bands on digest of miniprep? - (reply: 5)
  445. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  446. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  447. problem with plasmid extraction - (reply: 3)
  448. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  449. How to transfect two plasmids in a cell - (reply: 10)
  450. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  451. How to separate large size plasmids ~12kb - (reply: 8)
  452. low plasmid yield from midi preps - (reply: 4)
  453. Plasmid isolation, help with dilution step? - (reply: 7)
  454. full digestion of plasmid to get only dNTPs - (reply: 3)
  455. size limit of plasmid origins - (reply: 2)
  456. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  457. confusion with different forms of plasmid - (reply: 3)
  458. Plasmid as standard - Plasmid calculation (reply: 6)
  459. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  460. Effect of EtBr on plasmid supercoiling - (reply: 5)
  461. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  462. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  463. Digestion of a plasmid - (reply: 1)
  464. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  465. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  466. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  467. Amplifying plasmid - non specific binding of primer - (reply: 3)
  468. Storing plasmid at 4 degrees C - (reply: 5)
  469. Program to draw plasmids - draw plasmids (reply: 9)
  470. CIP required for double digestion of plasmid? - (reply: 3)
  471. Plasmid replication - Plasmid replication (reply: 13)
  472. Help with cloning a plasmid that contains repeats! - (reply: 2)
  473. Plasmid extraction sans commercial kits - (reply: 3)
  474. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  475. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  476. mutations in plasmids - (reply: 3)
  477. Sequential restriction digestion of plasmid - (reply: 58)
  478. miniprep and maxiprep - (reply: 9)
  479. plasmid spin column usage - (reply: 5)
  480. Stored bacteria lost plasmids? - (reply: 12)
  481. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  482. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  483. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  484. PLASMID ISOLATION - (reply: 10)
  485. Frustrated with Plasmid Preps - need help! - (reply: 14)
  486. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)