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Plasmid Related Discussions
  1. Doubt on HisTag protein purification miniprep - (reply: 3)
  2. Are relaxed plasmids origin of rep sensitive to bp substitution in RNA I/II sequ - (reply: 5)
  3. How stable is double digested plasmid - (reply: 6)
  4. low plasmid concentration - (reply: 1)
  5. What make a plasmid candidate for stable transfection - (reply: 1)
  6. Lower Plasmid Yields - (reply: 4)
  7. Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
  8. Question on plasmids - (reply: 4)
  9. *NO* DNA eluted from plasmid prep - Why? - (reply: 12)
  10. How much plasmid for transformation? - (reply: 8)
  11. HELP: From Plasmid Vector to Bacterial Chromosome - (reply: 2)
  12. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  13. plasmid repositories - (reply: 2)
  14. Why the insert become shorter after inserting into plasmid? - (reply: 2)
  15. real time qPCR standard range plasmid - (reply: 3)
  16. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  17. Low Yield Miniprep - (reply: 5)
  18. How to know the plasmid extracted are indeed the plasmid of interest? - (reply: 4)
  19. Plasmids recombining - (reply: 3)
  20. Plasmid Methylation - (reply: 3)
  21. Why are there two origin of replications in plasmids - (reply: 3)
  22. agarose electrophoresis gel has 500 bp bright smear after mini prep - (reply: 1)
  23. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  24. Plasmid Amplification Issue - (reply: 6)
  25. Why do we create own customised plasmid rather than buying iy? - (reply: 1)
  26. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  27. Can't isolate plasmid from cloned E.coli - (reply: 4)
  28. Denatured plasmid for pcr - (reply: 1)
  29. Using more transformed bacteria for midiprep - (reply: 2)
  30. Screen for F plasmid in e. coli - (reply: 1)
  31. Plasmid Linearization Problems - (reply: 9)
  32. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  33. plasmid construction - (reply: 2)
  34. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  35. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  36. ethanol precipitation plasmid DNA - (reply: 3)
  37. Plasmid storage conditions - (reply: 5)
  38. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  39. Temperature sensitivity of Plasmids - (reply: 1)
  40. New (free) plasmid mapping program - (reply: 4)
  41. removing part from plasmid - (reply: 18)
  42. Help with plasmid extraction and redissolving? - (reply: 5)
  43. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  44. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  45. Promoter sequence search in a plasmid map - (reply: 3)
  46. Heat vs pH during plasmid extraction - (reply: 8)
  47. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  48. Heating elution buffer for plasmid prep - (reply: 1)
  49. Using pBABE plasmids for regular transfection - (reply: 1)
  50. Rxn not working - amplify/linearize plasmid - (reply: 3)
  51. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  52. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  53. RNA contamination in plasmid extracted - (reply: 1)
  54. Problem in plasmid digestion after transformation - (reply: 4)
  55. Call for help! Plasmid extraction problem - (reply: 4)
  56. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  57. How can addgene sell plasmids with patented technology - (reply: 2)
  58. Transient transfection with large plasmid - (reply: 3)
  59. Midiprep from mini prep DNA - (reply: 2)
  60. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  61. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  62. Plasmids DNA on filter paper - (reply: 1)
  63. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  64. Storage life of plasmid DNA in water - (reply: 5)
  65. Plasmid DNA digestion problem - (reply: 3)
  66. free online DNA & plasmid editing tool - (reply: 1)
  67. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  68. Appearance of a salty plasmid preparation - (reply: 1)
  69. Transformation of Restricted plasmid - (reply: 3)
  70. Why is my insert missing from my plasmid? - (reply: 4)
  71. Plasmid digestion and gel purification using HindIII - (reply: 3)
  72. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  73. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  74. Colonies grown without plasmid - (reply: 7)
  75. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  76. Plasmid on blotting paper - (reply: 1)
  77. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  78. What plasmid sizes do you work with for SDM? - (reply: 1)
  79. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  80. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  81. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  82. Running undigested plasmids on gel - (reply: 2)
  83. Plasmid isolation from Enterococcus - (reply: 1)
  84. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  85. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  86. Whole plasmid amplification by PCR - (reply: 2)
  87. Sequencing of the cloned plasmid - (reply: 5)
  88. Plasmid DNA contamination - (reply: 5)
  89. Problems with plasmid DNA extraction - (reply: 3)
  90. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  91. Can I quantify restricted plasmid and oligos? - (reply: 3)
  92. conditionally active plasmids - (reply: 1)
  93. Qiagen mini prep cell fragment pellet - (reply: 2)
  94. Transient transfection of linearized plasmids - (reply: 3)
  95. Transfecting three plasmids into hES cells - (reply: 2)
  96. HUVEC plasmid transfection challenges - (reply: 2)
  97. Plasmid DNA - (reply: 3)
  98. Genomic DNA and plasmid DNA isolation - (reply: 3)
  99. faint band after plasmid extraction - (reply: 7)
  100. plasmid extraction troubleshooting,please help !! - (reply: 9)
  101. plasmid digestion - problem with enzymes? - (reply: 4)
  102. Plasmid stability in Ecoli during storage - (reply: 3)
  103. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  104. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  105. Plasmid isolation from gram positive bacteria - (reply: 2)
  106. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  107. problems with plasmid miniprep - (reply: 8)
  108. Determining the amount of plasmid transfected - (reply: 1)
  109. How to check for the presence of plasmid? - (reply: 3)
  110. PCR with Plasmid recovered from filter paper - (reply: 6)
  111. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  112. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  113. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  114. restricted PCR plasmid runs slower - (reply: 2)
  115. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
  116. Plasmid compatibly groups - (reply: 5)
  117. Plasmid isolation from agrobacterium - (reply: 1)
  118. Cutting my plasmid made it larger - (reply: 7)
  119. Recreate original plasmid by cutting out insert - (reply: 2)
  120. posting sequences of plasmid? - (reply: 3)
  121. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  122. problem with plasmid digestion - (reply: 5)
  123. unexpected band in PCR with plasmid - (reply: 4)
  124. Sequencing Primers and Plasmids - (reply: 3)
  125. Replication of the Plasmid DNA - (reply: 6)
  126. expression plasmids for tRNA - (reply: 6)
  127. cloning pcr insert into plasmid - (reply: 2)
  128. Re-culturing empty plasmid - (reply: 1)
  129. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
  130. Plasmid Miniprep - (reply: 2)
  131. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  132. Plasmid DNA at RT for a few days - (reply: 4)
  133. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  134. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  135. plasmid rearrangement or sabotage - (reply: 2)
  136. Linearising plasmid before transfection? - (reply: 2)
  137. Smear in plasmid sample - (reply: 9)
  138. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  139. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  140. cloning a gene in to a plasmid - (reply: 5)
  141. Characterizing an unknown plasmid - (reply: 6)
  142. Plasmid Purification and Digestion Problem - (reply: 2)
  143. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  144. QuikChange with Different plasmids - (reply: 1)
  145. Co-transfection with 2 plasmids - (reply: 10)
  146. Single Plasmid Gel Band - (reply: 1)
  147. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  148. Transformation with a big plasmid - (reply: 4)
  149. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  150. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  151. digested plasmid - (reply: 2)
  152. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  153. isolation of plasmid (midi and maxi prep) - (reply: 18)
  154. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  155. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  156. plasmid tetR and tet promoter - (reply: 2)
  157. Plasmid clone in BSP - (reply: 1)
  158. Swich selection in plasmid - (reply: 3)
  159. problems with midiprep - (reply: 3)
  160. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  161. Hypothetical Synthetic Plasmid - (reply: 9)
  162. How many fragments in plasmid DNA prep? - (reply: 1)
  163. Problem with plasmids transformation - (reply: 6)
  164. Calcium chloride plasmid transfection - (reply: 1)
  165. How Much Plasmid? - (reply: 6)
  166. Plasmid isolation - (reply: 1)
  167. plasmid;GI; ACESSION NUMBER - (reply: 2)
  168. virus transduction vs plasmid transfection - (reply: 4)
  169. Maxiprep of freeze/thawed pellet. - (reply: 1)
  170. Bizarre changes in plasmid band size - (reply: 3)
  171. Buffer composition for miniprep kit - (reply: 6)
  172. plasmid repository - (reply: 2)
  173. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  174. Falsely High Yields on Qiagen Miniprep? Assayed by Nanodrop - (reply: 3)
  175. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  176. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  177. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  178. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  179. Pls help me with this plasmid!!! - (reply: 1)
  180. Standard curve using plasmid - (reply: 1)
  181. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  182. Methods of plasmid transformation? only heat shock? - (reply: 1)
  183. No plasmid after a maxi prep mystery - (reply: 4)
  184. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  185. Co-expression of 2 plasmids in E.coli - (reply: 1)
  186. How to avoid plasmid instability - (reply: 4)
  187. Plasmid vs cDNA amplification by PCR - (reply: 2)
  188. Shear-induced damage of plasmid by vortexing - (reply: 1)
  189. RNase Band in old plasmid stored at -40 - (reply: 2)
  190. Plasmid vector for yeast and E. coli - (reply: 2)
  191. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  192. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  193. Plasmid digestion after transformation - (reply: 4)
  194. Puzzle about plamid and its digest, plz help - (reply: 8)
  195. Smear above plasmid dna - (reply: 3)
  196. result of plasmid extraction - (reply: 2)
  197. plasmid extraction - (reply: 5)
  198. Made the switch from Invitrogen Miniprep to Promega PureYield. What's going - (reply: 1)
  199. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  200. plasmid extraction - (reply: 3)
  201. No DNA after midiprep - (reply: 2)
  202. Is that a Plasmid??? - (reply: 5)
  203. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  204. TRADITIONAL METHOD FOR PLASMIDEXTRACTION - (reply: 1)
  205. plasmid extraction - (reply: 13)
  206. Cloning two genes in the same plasmid - (reply: 4)
  207. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  208. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  209. Plasmid, gene orientation, insert orientation. - (reply: 1)
  210. Plasmid recovery problem - (reply: 3)
  211. Electroporated plasmid loses the insert - (reply: 8)
  212. Plasmid sequencing without primers - (reply: 1)
  213. RE site on complimentary strand of plasmid? - (reply: 1)
  214. How much of plasmid should be cut to see the gene - (reply: 1)
  215. Plasmid sequencing - (reply: 1)
  216. Plasmid isolation Qiagen - (reply: 2)
  217. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  218. Amplify fragment in plasmid? - (reply: 4)
  219. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  220. failure in miniprep - (reply: 1)
  221. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  222. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  223. Template DNA (plasmid) concentration for PCR - (reply: 4)
  224. co-expression of 2 plasmids in E.coli - (reply: 3)
  225. How to choose the right plasmids for certain types of cell? - (reply: 1)
  226. Plasmid digestion create a bigger plasmid - (reply: 1)
  227. whole plasmid pcr? - (reply: 6)
  228. Looking for a GFP plasmid (non-amp) - (reply: 3)
  229. turbid DNA from maxiprep - (reply: 3)
  230. extract plasmids from transfected eukaryotic cells - (reply: 6)
  231. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  232. Plasmid compatible with pBAD24 - (reply: 1)
  233. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  234. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  235. Endofree Maxiprep tube volume - (reply: 6)
  236. Miniprep OD beyond 2.00 - Troubleshooting-help desperately needed - (reply: 10)
  237. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  238. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  239. Low Miniprep Yield - (reply: 1)
  240. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  241. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  242. Problem with maxiprep - (reply: 2)
  243. ~200kb Plasmid DNA purification is not working - (reply: 1)
  244. ligating a 6 His tag to plasmid - (reply: 2)
  245. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  246. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  247. Plasmid sequencing failed why? - (reply: 10)
  248. Mini prep of frozen bacteria cells - (reply: 2)
  249. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  250. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  251. SLIM mutagenesis of large plasmids - (reply: 8)
  252. why 12 h culture for plasmid prep? - (reply: 2)
  253. Big plasmids and Transfection - (reply: 3)
  254. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  255. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  256. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  257. pcr amplify an insert in plasmid? - (reply: 2)
  258. odd plasmids - (reply: 2)
  259. Lactobacillus plasmid - (reply: 1)
  260. Will transfected plasmids induce cytokine production? - (reply: 1)
  261. Question about plasmid midiprep - (reply: 1)
  262. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  263. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  264. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  265. Overexpression plasmid is not expressing protein - (reply: 13)
  266. Extra band on gel after miniprep - (reply: 6)
  267. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  268. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  269. PCR problem - basic PCR for plasmid amplification (reply: 2)
  270. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  271. Help Miniprep kit SIGMA - (reply: 2)
  272. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  273. Low yields of plasmids from miniprep - (reply: 1)
  274. A gene is not inserted into the Plasmid - (reply: 2)
  275. Plasmid Question - (reply: 4)
  276. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  277. Weird migration of double digested plasmid - (reply: 4)
  278. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  279. Help for co-transfection of proteins and plasmid - (reply: 1)
  280. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  281. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  282. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  283. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  284. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  285. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  286. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  287. Undigestable/unrestrictable Plasmid? - (reply: 1)
  288. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  289. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  290. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  291. problems encountered when working on a large plasmid - (reply: 2)
  292. make a Mega plasmid mutation - (reply: 1)
  293. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  294. Plasmid standard problem - (reply: 2)
  295. Do plasmids work in bacteria? - (reply: 1)
  296. CEN sequences on plasmids (yeast) - (reply: 4)
  297. Plasmid out of BL21 for mutagensis - (reply: 3)
  298. ORI names - name of a plasmid ori (reply: 11)
  299. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
  300. Change origin of a plasmid - (reply: 4)
  301. Better to sequence from plasmid? - (reply: 5)
  302. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  303. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  304. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  305. problem with pLK01 plasmid ligation - (reply: 2)
  306. Mini prep streaking - basic question (reply: 3)
  307. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  308. Maxiprep reagents - (reply: 5)
  309. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  310. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  311. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  312. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  313. PCR mutagenesis of plasmid - (reply: 5)
  314. Guanadine HCl for plasmid miniprep - (reply: 3)
  315. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  316. Not getting plasmid from miniprep - (reply: 7)
  317. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  318. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  319. Digesting large amount of plasmid - (reply: 4)
  320. Miniprep and sequencing - (reply: 4)
  321. Cloning <100 bp fragments into a plasmid - (reply: 7)
  322. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  323. Help! my plasmid size changed after ligation - (reply: 5)
  324. Electroporation doesn't like my plasmid??? - (reply: 4)
  325. Cloning in pCR2.1 plasmid - (reply: 7)
  326. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  327. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
  328. Miniprep DNA-strange nanodrop peak at 240-250nm? - (reply: 4)
  329. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  330. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  331. Cloning: Randomly recombined Plasmids? - (reply: 6)
  332. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  333. plasmid + sepharose - how do this work? (reply: 1)
  334. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  335. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  336. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  337. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  338. Plasmid size problems! - (reply: 3)
  339. Left DNA plasmid out for a week - (reply: 3)
  340. Transfecting primary cells with plasmid - (reply: 1)
  341. Extra bands after QIAGEN spin miniprep - (reply: 3)
  342. question about plasmid extraction - (reply: 2)
  343. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  344. Disappearing plasmid - (reply: 5)
  345. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  346. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  347. problem in plasmid transfection - (reply: 2)
  348. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  349. Plasmid DNA mini-prep - no good isolation (reply: 5)
  350. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  351. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  352. plasmid extraction - why dilute (reply: 3)
  353. very high molecular weight plasmid - (reply: 6)
  354. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  355. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  356. Concepts behind plasmid prep - (reply: 2)
  357. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  358. PCR off plasmid for screening - (reply: 2)
  359. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  360. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  361. Plasmid frameshift problem after clonning - (reply: 2)
  362. plasmid extraction kit wash buffer solution - (reply: 3)
  363. plasmid extraction kit solution composition - wash buffer (reply: 2)
  364. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  365. are plasmids used for lab work methylated - (reply: 1)
  366. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  367. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
  368. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  369. Isolation of protein crosslinked to plasmid - (reply: 3)
  370. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  371. Which Plasmid DNA for standard curve? - (reply: 5)
  372. Floating white pellet in miniprep - (reply: 2)
  373. Plasmid problem - From PCR product (reply: 7)
  374. Transfection - Plasmid Uptake Control - (reply: 2)
  375. Culture Grow well but No Plasmid - (reply: 2)
  376. software for plasmid drawing - (reply: 13)
  377. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  378. plasmid purification kit for transfection - (reply: 2)
  379. best plasmid purification kit for transfection - (reply: 1)
  380. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  381. Checking Plasmid Expression - (reply: 1)
  382. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  383. PCR a plasmid protein - (reply: 2)
  384. plasmid DNA disappear in the minipre - (reply: 2)
  385. Interpreting a Plasmid Profile gel photo - (reply: 3)
  386. marker swap plasmid - (reply: 4)
  387. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  388. plasmid dna isolation - (reply: 1)
  389. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  390. siRNA and plasmid cotransfection - (reply: 1)
  391. rna from plasmid - (reply: 1)
  392. Miniprep - (reply: 3)
  393. miniPrep troubleshooting - (reply: 7)
  394. Mini prep - (reply: 4)
  395. Plasmid curing method - (reply: 4)
  396. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  397. plasmid DNA lab question - (reply: 11)
  398. Problem amplifying plasmid - (reply: 1)
  399. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  400. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  401. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  402. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  403. Transport of miniprep - (reply: 2)
  404. role of glucose in plasmid isolation? - (reply: 1)
  405. sequencing clones after BSP : need miniprep ?? - (reply: 1)
  406. Plasmids for protein expression in mammalian cells - (reply: 2)
  407. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  408. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  409. plasmid, BAC, or cosmid? - (reply: 3)
  410. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  411. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  412. Mutation during transformation/maxiprep - (reply: 1)
  413. Best plasmid miniprep? - (reply: 13)
  414. Plasmid instability in E.coli BL21? - (reply: 3)
  415. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  416. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  417. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  418. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  419. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  420. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  421. Plasmid linearization by PCR - (reply: 4)
  422. plasmid sequence identification problem - (reply: 2)
  423. plasmid construct problem - (reply: 3)
  424. How to get plasmid maps from publications? - (reply: 5)
  425. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  426. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  427. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  428. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  429. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  430. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  431. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  432. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  433. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  434. Plasmid sequence - (reply: 1)
  435. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  436. plasmid isolation - (reply: 4)
  437. Plasmid supercoiling affecting PCR? - (reply: 2)
  438. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  439. strange extra bands on digest of miniprep? - (reply: 5)
  440. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  441. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  442. problem with plasmid extraction - (reply: 3)
  443. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  444. How to transfect two plasmids in a cell - (reply: 10)
  445. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  446. How to separate large size plasmids ~12kb - (reply: 8)
  447. low plasmid yield from midi preps - (reply: 4)
  448. Plasmid isolation, help with dilution step? - (reply: 7)
  449. full digestion of plasmid to get only dNTPs - (reply: 3)
  450. size limit of plasmid origins - (reply: 2)
  451. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  452. confusion with different forms of plasmid - (reply: 3)
  453. Plasmid as standard - Plasmid calculation (reply: 6)
  454. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  455. Effect of EtBr on plasmid supercoiling - (reply: 5)
  456. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  457. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  458. Digestion of a plasmid - (reply: 1)
  459. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  460. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  461. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  462. Amplifying plasmid - non specific binding of primer - (reply: 3)
  463. Storing plasmid at 4 degrees C - (reply: 5)
  464. Program to draw plasmids - draw plasmids (reply: 9)
  465. CIP required for double digestion of plasmid? - (reply: 3)
  466. Plasmid replication - Plasmid replication (reply: 13)
  467. Help with cloning a plasmid that contains repeats! - (reply: 2)
  468. Plasmid extraction sans commercial kits - (reply: 3)
  469. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  470. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  471. mutations in plasmids - (reply: 3)
  472. Sequential restriction digestion of plasmid - (reply: 58)
  473. miniprep and maxiprep - (reply: 9)
  474. plasmid spin column usage - (reply: 5)
  475. Stored bacteria lost plasmids? - (reply: 12)
  476. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  477. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  478. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  479. PLASMID ISOLATION - (reply: 10)
  480. Frustrated with Plasmid Preps - need help! - (reply: 14)
  481. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)