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Plasmid Related Discussions
  1. Why the insert become shorter after inserting into plasmid? - (reply: 1)
  2. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  3. Low Yield Miniprep - (reply: 5)
  4. How to know the plasmid extracted are indeed the plasmid of interest? - (reply: 6)
  5. Plasmids recombining - (reply: 3)
  6. Plasmid Methylation - (reply: 3)
  7. Why are there two origin of replications in plasmids - (reply: 3)
  8. agarose electrophoresis gel has 500 bp bright smear after mini prep - (reply: 1)
  9. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  10. Plasmid Amplification Issue - (reply: 6)
  11. Why do we create own customised plasmid rather than buying iy? - (reply: 1)
  12. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  13. Can't isolate plasmid from cloned E.coli - (reply: 3)
  14. Denatured plasmid for pcr - (reply: 1)
  15. Using more transformed bacteria for midiprep - (reply: 2)
  16. Screen for F plasmid in e. coli - (reply: 1)
  17. Plasmid Linearization Problems - (reply: 9)
  18. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  19. plasmid construction - (reply: 2)
  20. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  21. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  22. ethanol precipitation plasmid DNA - (reply: 3)
  23. Plasmid storage conditions - (reply: 3)
  24. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  25. Temperature sensitivity of Plasmids - (reply: 1)
  26. New (free) plasmid mapping program - (reply: 4)
  27. removing part from plasmid - (reply: 18)
  28. Help with plasmid extraction and redissolving? - (reply: 5)
  29. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  30. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  31. Promoter sequence search in a plasmid map - (reply: 3)
  32. Heat vs pH during plasmid extraction - (reply: 8)
  33. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  34. Heating elution buffer for plasmid prep - (reply: 1)
  35. Using pBABE plasmids for regular transfection - (reply: 1)
  36. Rxn not working - amplify/linearize plasmid - (reply: 3)
  37. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  38. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  39. RNA contamination in plasmid extracted - (reply: 1)
  40. Problem in plasmid digestion after transformation - (reply: 4)
  41. Call for help! Plasmid extraction problem - (reply: 4)
  42. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  43. How can addgene sell plasmids with patented technology - (reply: 2)
  44. Transient transfection with large plasmid - (reply: 3)
  45. Midiprep from mini prep DNA - (reply: 2)
  46. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  47. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  48. Plasmids DNA on filter paper - (reply: 1)
  49. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  50. Storage life of plasmid DNA in water - (reply: 5)
  51. Plasmid DNA digestion problem - (reply: 3)
  52. free online DNA & plasmid editing tool - (reply: 1)
  53. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  54. Appearance of a salty plasmid preparation - (reply: 1)
  55. Transformation of Restricted plasmid - (reply: 3)
  56. Why is my insert missing from my plasmid? - (reply: 4)
  57. Plasmid digestion and gel purification using HindIII - (reply: 3)
  58. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  59. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  60. Colonies grown without plasmid - (reply: 7)
  61. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  62. Plasmid on blotting paper - (reply: 1)
  63. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  64. What plasmid sizes do you work with for SDM? - (reply: 1)
  65. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  66. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  67. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  68. Running undigested plasmids on gel - (reply: 2)
  69. Plasmid isolation from Enterococcus - (reply: 1)
  70. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  71. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  72. Whole plasmid amplification by PCR - (reply: 2)
  73. Sequencing of the cloned plasmid - (reply: 5)
  74. Plasmid DNA contamination - (reply: 5)
  75. Problems with plasmid DNA extraction - (reply: 3)
  76. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  77. Can I quantify restricted plasmid and oligos? - (reply: 3)
  78. conditionally active plasmids - (reply: 1)
  79. Qiagen mini prep cell fragment pellet - (reply: 2)
  80. Transient transfection of linearized plasmids - (reply: 3)
  81. Transfecting three plasmids into hES cells - (reply: 2)
  82. HUVEC plasmid transfection challenges - (reply: 2)
  83. Plasmid DNA - (reply: 3)
  84. Genomic DNA and plasmid DNA isolation - (reply: 3)
  85. faint band after plasmid extraction - (reply: 7)
  86. plasmid extraction troubleshooting,please help !! - (reply: 9)
  87. plasmid digestion - problem with enzymes? - (reply: 4)
  88. Plasmid stability in Ecoli during storage - (reply: 3)
  89. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  90. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  91. Plasmid isolation from gram positive bacteria - (reply: 2)
  92. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  93. problems with plasmid miniprep - (reply: 8)
  94. Determining the amount of plasmid transfected - (reply: 1)
  95. How to check for the presence of plasmid? - (reply: 3)
  96. PCR with Plasmid recovered from filter paper - (reply: 6)
  97. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  98. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  99. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  100. restricted PCR plasmid runs slower - (reply: 2)
  101. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
  102. Plasmid compatibly groups - (reply: 5)
  103. Plasmid isolation from agrobacterium - (reply: 1)
  104. Cutting my plasmid made it larger - (reply: 7)
  105. Recreate original plasmid by cutting out insert - (reply: 2)
  106. posting sequences of plasmid? - (reply: 3)
  107. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  108. problem with plasmid digestion - (reply: 5)
  109. unexpected band in PCR with plasmid - (reply: 4)
  110. Sequencing Primers and Plasmids - (reply: 3)
  111. Replication of the Plasmid DNA - (reply: 6)
  112. expression plasmids for tRNA - (reply: 6)
  113. cloning pcr insert into plasmid - (reply: 2)
  114. Re-culturing empty plasmid - (reply: 1)
  115. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
  116. Plasmid Miniprep - (reply: 2)
  117. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  118. Plasmid DNA at RT for a few days - (reply: 4)
  119. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  120. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  121. plasmid rearrangement or sabotage - (reply: 2)
  122. Linearising plasmid before transfection? - (reply: 2)
  123. Smear in plasmid sample - (reply: 9)
  124. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  125. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  126. cloning a gene in to a plasmid - (reply: 5)
  127. Characterizing an unknown plasmid - (reply: 6)
  128. Plasmid Purification and Digestion Problem - (reply: 2)
  129. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  130. QuikChange with Different plasmids - (reply: 1)
  131. Co-transfection with 2 plasmids - (reply: 10)
  132. Single Plasmid Gel Band - (reply: 1)
  133. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  134. Transformation with a big plasmid - (reply: 4)
  135. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  136. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  137. digested plasmid - (reply: 2)
  138. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  139. isolation of plasmid (midi and maxi prep) - (reply: 18)
  140. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  141. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  142. plasmid tetR and tet promoter - (reply: 2)
  143. Plasmid clone in BSP - (reply: 1)
  144. Swich selection in plasmid - (reply: 3)
  145. problems with midiprep - (reply: 3)
  146. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  147. Hypothetical Synthetic Plasmid - (reply: 9)
  148. How many fragments in plasmid DNA prep? - (reply: 1)
  149. Problem with plasmids transformation - (reply: 6)
  150. Calcium chloride plasmid transfection - (reply: 1)
  151. How Much Plasmid? - (reply: 6)
  152. Plasmid isolation - (reply: 1)
  153. plasmid;GI; ACESSION NUMBER - (reply: 2)
  154. virus transduction vs plasmid transfection - (reply: 4)
  155. Maxiprep of freeze/thawed pellet. - (reply: 1)
  156. Bizarre changes in plasmid band size - (reply: 3)
  157. Buffer composition for miniprep kit - (reply: 6)
  158. plasmid repository - (reply: 2)
  159. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  160. Falsely High Yields on Qiagen Miniprep? Assayed by Nanodrop - (reply: 3)
  161. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  162. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  163. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  164. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  165. Pls help me with this plasmid!!! - (reply: 1)
  166. Standard curve using plasmid - (reply: 1)
  167. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  168. Methods of plasmid transformation? only heat shock? - (reply: 1)
  169. No plasmid after a maxi prep mystery - (reply: 4)
  170. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  171. Co-expression of 2 plasmids in E.coli - (reply: 1)
  172. How to avoid plasmid instability - (reply: 4)
  173. Plasmid vs cDNA amplification by PCR - (reply: 2)
  174. Shear-induced damage of plasmid by vortexing - (reply: 1)
  175. RNase Band in old plasmid stored at -40 - (reply: 2)
  176. Plasmid vector for yeast and E. coli - (reply: 2)
  177. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  178. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  179. Plasmid digestion after transformation - (reply: 4)
  180. Puzzle about plamid and its digest, plz help - (reply: 8)
  181. Smear above plasmid dna - (reply: 3)
  182. result of plasmid extraction - (reply: 2)
  183. plasmid extraction - (reply: 5)
  184. Made the switch from Invitrogen Miniprep to Promega PureYield. What's going - (reply: 1)
  185. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  186. plasmid extraction - (reply: 3)
  187. No DNA after midiprep - (reply: 2)
  188. Is that a Plasmid??? - (reply: 5)
  189. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  190. TRADITIONAL METHOD FOR PLASMIDEXTRACTION - (reply: 1)
  191. plasmid extraction - (reply: 13)
  192. Cloning two genes in the same plasmid - (reply: 4)
  193. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  194. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  195. Plasmid, gene orientation, insert orientation. - (reply: 1)
  196. Plasmid recovery problem - (reply: 3)
  197. Electroporated plasmid loses the insert - (reply: 8)
  198. Plasmid sequencing without primers - (reply: 1)
  199. RE site on complimentary strand of plasmid? - (reply: 1)
  200. How much of plasmid should be cut to see the gene - (reply: 1)
  201. Plasmid sequencing - (reply: 1)
  202. Plasmid isolation Qiagen - (reply: 2)
  203. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  204. Amplify fragment in plasmid? - (reply: 4)
  205. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  206. failure in miniprep - (reply: 1)
  207. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  208. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  209. Template DNA (plasmid) concentration for PCR - (reply: 4)
  210. co-expression of 2 plasmids in E.coli - (reply: 3)
  211. How to choose the right plasmids for certain types of cell? - (reply: 1)
  212. Plasmid digestion create a bigger plasmid - (reply: 1)
  213. whole plasmid pcr? - (reply: 6)
  214. Looking for a GFP plasmid (non-amp) - (reply: 3)
  215. turbid DNA from maxiprep - (reply: 3)
  216. extract plasmids from transfected eukaryotic cells - (reply: 6)
  217. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  218. Plasmid compatible with pBAD24 - (reply: 1)
  219. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  220. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  221. Endofree Maxiprep tube volume - (reply: 6)
  222. Miniprep OD beyond 2.00 - Troubleshooting-help desperately needed - (reply: 10)
  223. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  224. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  225. Low Miniprep Yield - (reply: 1)
  226. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  227. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  228. Problem with maxiprep - (reply: 2)
  229. ~200kb Plasmid DNA purification is not working - (reply: 1)
  230. ligating a 6 His tag to plasmid - (reply: 2)
  231. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  232. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  233. Plasmid sequencing failed why? - (reply: 10)
  234. Mini prep of frozen bacteria cells - (reply: 2)
  235. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  236. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  237. SLIM mutagenesis of large plasmids - (reply: 8)
  238. why 12 h culture for plasmid prep? - (reply: 2)
  239. Big plasmids and Transfection - (reply: 3)
  240. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  241. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  242. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  243. pcr amplify an insert in plasmid? - (reply: 2)
  244. odd plasmids - (reply: 2)
  245. Lactobacillus plasmid - (reply: 1)
  246. Will transfected plasmids induce cytokine production? - (reply: 1)
  247. Question about plasmid midiprep - (reply: 1)
  248. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  249. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  250. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  251. Overexpression plasmid is not expressing protein - (reply: 13)
  252. Extra band on gel after miniprep - (reply: 6)
  253. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  254. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  255. PCR problem - basic PCR for plasmid amplification (reply: 2)
  256. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  257. Help Miniprep kit SIGMA - (reply: 2)
  258. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  259. Low yields of plasmids from miniprep - (reply: 1)
  260. A gene is not inserted into the Plasmid - (reply: 2)
  261. Plasmid Question - (reply: 4)
  262. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  263. Weird migration of double digested plasmid - (reply: 4)
  264. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  265. Help for co-transfection of proteins and plasmid - (reply: 1)
  266. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  267. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  268. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  269. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  270. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  271. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  272. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  273. Undigestable/unrestrictable Plasmid? - (reply: 1)
  274. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  275. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  276. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  277. problems encountered when working on a large plasmid - (reply: 2)
  278. make a Mega plasmid mutation - (reply: 1)
  279. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  280. Plasmid standard problem - (reply: 2)
  281. Do plasmids work in bacteria? - (reply: 1)
  282. CEN sequences on plasmids (yeast) - (reply: 4)
  283. Plasmid out of BL21 for mutagensis - (reply: 3)
  284. ORI names - name of a plasmid ori (reply: 11)
  285. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
  286. Change origin of a plasmid - (reply: 4)
  287. Better to sequence from plasmid? - (reply: 5)
  288. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  289. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  290. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  291. problem with pLK01 plasmid ligation - (reply: 2)
  292. Mini prep streaking - basic question (reply: 3)
  293. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  294. Maxiprep reagents - (reply: 5)
  295. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  296. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  297. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  298. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  299. PCR mutagenesis of plasmid - (reply: 5)
  300. Guanadine HCl for plasmid miniprep - (reply: 3)
  301. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  302. Not getting plasmid from miniprep - (reply: 7)
  303. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  304. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  305. Digesting large amount of plasmid - (reply: 4)
  306. Miniprep and sequencing - (reply: 4)
  307. Cloning <100 bp fragments into a plasmid - (reply: 7)
  308. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  309. Help! my plasmid size changed after ligation - (reply: 5)
  310. Electroporation doesn't like my plasmid??? - (reply: 4)
  311. Cloning in pCR2.1 plasmid - (reply: 7)
  312. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  313. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
  314. Miniprep DNA-strange nanodrop peak at 240-250nm? - (reply: 4)
  315. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  316. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  317. Cloning: Randomly recombined Plasmids? - (reply: 6)
  318. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  319. plasmid + sepharose - how do this work? (reply: 1)
  320. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  321. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  322. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  323. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  324. Plasmid size problems! - (reply: 3)
  325. Left DNA plasmid out for a week - (reply: 3)
  326. Transfecting primary cells with plasmid - (reply: 1)
  327. Extra bands after QIAGEN spin miniprep - (reply: 3)
  328. question about plasmid extraction - (reply: 2)
  329. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  330. Disappearing plasmid - (reply: 5)
  331. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  332. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  333. problem in plasmid transfection - (reply: 2)
  334. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  335. Plasmid DNA mini-prep - no good isolation (reply: 5)
  336. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  337. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  338. plasmid extraction - why dilute (reply: 3)
  339. very high molecular weight plasmid - (reply: 6)
  340. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  341. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  342. Concepts behind plasmid prep - (reply: 2)
  343. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  344. PCR off plasmid for screening - (reply: 2)
  345. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  346. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  347. Plasmid frameshift problem after clonning - (reply: 2)
  348. plasmid extraction kit wash buffer solution - (reply: 3)
  349. plasmid extraction kit solution composition - wash buffer (reply: 2)
  350. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  351. are plasmids used for lab work methylated - (reply: 1)
  352. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  353. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
  354. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  355. Isolation of protein crosslinked to plasmid - (reply: 3)
  356. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  357. Which Plasmid DNA for standard curve? - (reply: 5)
  358. Floating white pellet in miniprep - (reply: 2)
  359. Plasmid problem - From PCR product (reply: 7)
  360. Transfection - Plasmid Uptake Control - (reply: 2)
  361. Culture Grow well but No Plasmid - (reply: 2)
  362. software for plasmid drawing - (reply: 12)
  363. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  364. plasmid purification kit for transfection - (reply: 2)
  365. best plasmid purification kit for transfection - (reply: 1)
  366. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  367. Checking Plasmid Expression - (reply: 1)
  368. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  369. PCR a plasmid protein - (reply: 2)
  370. plasmid DNA disappear in the minipre - (reply: 2)
  371. Interpreting a Plasmid Profile gel photo - (reply: 3)
  372. marker swap plasmid - (reply: 4)
  373. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  374. plasmid dna isolation - (reply: 1)
  375. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  376. siRNA and plasmid cotransfection - (reply: 1)
  377. rna from plasmid - (reply: 1)
  378. Miniprep - (reply: 3)
  379. miniPrep troubleshooting - (reply: 7)
  380. Mini prep - (reply: 4)
  381. Plasmid curing method - (reply: 4)
  382. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  383. plasmid DNA lab question - (reply: 11)
  384. Problem amplifying plasmid - (reply: 1)
  385. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  386. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  387. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  388. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  389. Transport of miniprep - (reply: 2)
  390. role of glucose in plasmid isolation? - (reply: 1)
  391. sequencing clones after BSP : need miniprep ?? - (reply: 1)
  392. Plasmids for protein expression in mammalian cells - (reply: 2)
  393. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  394. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  395. plasmid, BAC, or cosmid? - (reply: 3)
  396. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  397. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  398. Mutation during transformation/maxiprep - (reply: 1)
  399. Best plasmid miniprep? - (reply: 13)
  400. Plasmid instability in E.coli BL21? - (reply: 3)
  401. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  402. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  403. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  404. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  405. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  406. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  407. Plasmid linearization by PCR - (reply: 4)
  408. plasmid sequence identification problem - (reply: 2)
  409. plasmid construct problem - (reply: 3)
  410. How to get plasmid maps from publications? - (reply: 5)
  411. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  412. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  413. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  414. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  415. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  416. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  417. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  418. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  419. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  420. Plasmid sequence - (reply: 1)
  421. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  422. plasmid isolation - (reply: 4)
  423. Plasmid supercoiling affecting PCR? - (reply: 2)
  424. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  425. strange extra bands on digest of miniprep? - (reply: 5)
  426. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  427. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  428. problem with plasmid extraction - (reply: 3)
  429. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  430. How to transfect two plasmids in a cell - (reply: 10)
  431. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  432. How to separate large size plasmids ~12kb - (reply: 8)
  433. low plasmid yield from midi preps - (reply: 4)
  434. Plasmid isolation, help with dilution step? - (reply: 7)
  435. full digestion of plasmid to get only dNTPs - (reply: 3)
  436. size limit of plasmid origins - (reply: 2)
  437. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  438. confusion with different forms of plasmid - (reply: 3)
  439. Plasmid as standard - Plasmid calculation (reply: 6)
  440. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  441. Effect of EtBr on plasmid supercoiling - (reply: 5)
  442. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  443. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  444. Digestion of a plasmid - (reply: 1)
  445. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  446. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  447. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  448. Amplifying plasmid - non specific binding of primer - (reply: 3)
  449. Storing plasmid at 4 degrees C - (reply: 5)
  450. Program to draw plasmids - draw plasmids (reply: 7)
  451. CIP required for double digestion of plasmid? - (reply: 3)
  452. Plasmid replication - Plasmid replication (reply: 13)
  453. Help with cloning a plasmid that contains repeats! - (reply: 2)
  454. Plasmid extraction sans commercial kits - (reply: 3)
  455. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  456. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  457. mutations in plasmids - (reply: 3)
  458. Sequential restriction digestion of plasmid - (reply: 58)
  459. miniprep and maxiprep - (reply: 9)
  460. plasmid spin column usage - (reply: 5)
  461. Stored bacteria lost plasmids? - (reply: 12)
  462. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  463. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  464. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  465. PLASMID ISOLATION - (reply: 10)
  466. Frustrated with Plasmid Preps - need help! - (reply: 14)
  467. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)