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Plasmid Related Discussions
  1. How much plasmid for transformation? - (reply: 7)
  2. HELP: From Plasmid Vector to Bacterial Chromosome - (reply: 2)
  3. The right lentivirus plasmid for overexpression in human cells - (reply: 3)
  4. plasmid repositories - (reply: 2)
  5. Why the insert become shorter after inserting into plasmid? - (reply: 2)
  6. real time qPCR standard range plasmid - (reply: 3)
  7. Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna - (reply: 6)
  8. Low Yield Miniprep - (reply: 5)
  9. How to know the plasmid extracted are indeed the plasmid of interest? - (reply: 6)
  10. Plasmids recombining - (reply: 3)
  11. Plasmid Methylation - (reply: 3)
  12. Why are there two origin of replications in plasmids - (reply: 3)
  13. agarose electrophoresis gel has 500 bp bright smear after mini prep - (reply: 1)
  14. Low plasmid yield after Miniprep, DNA in the first wash flow through (PB) - (reply: 1)
  15. Plasmid Amplification Issue - (reply: 6)
  16. Why do we create own customised plasmid rather than buying iy? - (reply: 1)
  17. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  18. Can't isolate plasmid from cloned E.coli - (reply: 4)
  19. Denatured plasmid for pcr - (reply: 1)
  20. Using more transformed bacteria for midiprep - (reply: 2)
  21. Screen for F plasmid in e. coli - (reply: 1)
  22. Plasmid Linearization Problems - (reply: 9)
  23. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
  24. plasmid construction - (reply: 2)
  25. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  26. Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
  27. ethanol precipitation plasmid DNA - (reply: 3)
  28. Plasmid storage conditions - (reply: 3)
  29. Miniprep gives high yield but maxiprep gives no plasmid? - (reply: 2)
  30. Temperature sensitivity of Plasmids - (reply: 1)
  31. New (free) plasmid mapping program - (reply: 4)
  32. removing part from plasmid - (reply: 18)
  33. Help with plasmid extraction and redissolving? - (reply: 5)
  34. Nanodrop machine error message when measuring plasmid DNA concentration - (reply: 3)
  35. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  36. Promoter sequence search in a plasmid map - (reply: 3)
  37. Heat vs pH during plasmid extraction - (reply: 8)
  38. Sterility issues with plasmid reconstitution buffer? - (reply: 3)
  39. Heating elution buffer for plasmid prep - (reply: 1)
  40. Using pBABE plasmids for regular transfection - (reply: 1)
  41. Rxn not working - amplify/linearize plasmid - (reply: 3)
  42. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  43. Gateway cloning-modifications to "entry" plasmid - (reply: 4)
  44. RNA contamination in plasmid extracted - (reply: 1)
  45. Problem in plasmid digestion after transformation - (reply: 4)
  46. Call for help! Plasmid extraction problem - (reply: 4)
  47. E. coli growing in LB-ampicillin medium without plasmid? Or miniprep problem? - (reply: 2)
  48. How can addgene sell plasmids with patented technology - (reply: 2)
  49. Transient transfection with large plasmid - (reply: 3)
  50. Midiprep from mini prep DNA - (reply: 2)
  51. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  52. Can one use circular plasmid that contains cDNA of interest as a template - (reply: 3)
  53. Plasmids DNA on filter paper - (reply: 1)
  54. Pectobacterium wasabiae and pCP20 plasmid - (reply: 6)
  55. Storage life of plasmid DNA in water - (reply: 5)
  56. Plasmid DNA digestion problem - (reply: 3)
  57. free online DNA & plasmid editing tool - (reply: 1)
  58. Trouble isolating plasmid from Pseudomonas aeruginosa - (reply: 3)
  59. Appearance of a salty plasmid preparation - (reply: 1)
  60. Transformation of Restricted plasmid - (reply: 3)
  61. Why is my insert missing from my plasmid? - (reply: 4)
  62. Plasmid digestion and gel purification using HindIII - (reply: 3)
  63. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  64. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  65. Colonies grown without plasmid - (reply: 7)
  66. Reconstituting plasmid DNA from blotting paper - water vs. TE buffer - (reply: 6)
  67. Plasmid on blotting paper - (reply: 1)
  68. Is it possible that the undigested version of this plasmid will be above the lin - (reply: 1)
  69. What plasmid sizes do you work with for SDM? - (reply: 1)
  70. Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
  71. Plasmid Purification Using an Alkaline Lysis - (reply: 1)
  72. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  73. Running undigested plasmids on gel - (reply: 2)
  74. Plasmid isolation from Enterococcus - (reply: 1)
  75. Plasmid Vector - CMV enhancer / promoter fluorescent reporter, a positive or neg - (reply: 3)
  76. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  77. Whole plasmid amplification by PCR - (reply: 2)
  78. Sequencing of the cloned plasmid - (reply: 5)
  79. Plasmid DNA contamination - (reply: 5)
  80. Problems with plasmid DNA extraction - (reply: 3)
  81. What are the elements of a BAC that differentiate it from a regular plasmid - (reply: 1)
  82. Can I quantify restricted plasmid and oligos? - (reply: 3)
  83. conditionally active plasmids - (reply: 1)
  84. Qiagen mini prep cell fragment pellet - (reply: 2)
  85. Transient transfection of linearized plasmids - (reply: 3)
  86. Transfecting three plasmids into hES cells - (reply: 2)
  87. HUVEC plasmid transfection challenges - (reply: 2)
  88. Plasmid DNA - (reply: 3)
  89. Genomic DNA and plasmid DNA isolation - (reply: 3)
  90. faint band after plasmid extraction - (reply: 7)
  91. plasmid extraction troubleshooting,please help !! - (reply: 9)
  92. plasmid digestion - problem with enzymes? - (reply: 4)
  93. Plasmid stability in Ecoli during storage - (reply: 3)
  94. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  95. Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
  96. Plasmid isolation from gram positive bacteria - (reply: 2)
  97. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  98. problems with plasmid miniprep - (reply: 8)
  99. Determining the amount of plasmid transfected - (reply: 1)
  100. How to check for the presence of plasmid? - (reply: 3)
  101. PCR with Plasmid recovered from filter paper - (reply: 6)
  102. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  103. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  104. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  105. restricted PCR plasmid runs slower - (reply: 2)
  106. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
  107. Plasmid compatibly groups - (reply: 5)
  108. Plasmid isolation from agrobacterium - (reply: 1)
  109. Cutting my plasmid made it larger - (reply: 7)
  110. Recreate original plasmid by cutting out insert - (reply: 2)
  111. posting sequences of plasmid? - (reply: 3)
  112. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  113. problem with plasmid digestion - (reply: 5)
  114. unexpected band in PCR with plasmid - (reply: 4)
  115. Sequencing Primers and Plasmids - (reply: 3)
  116. Replication of the Plasmid DNA - (reply: 6)
  117. expression plasmids for tRNA - (reply: 6)
  118. cloning pcr insert into plasmid - (reply: 2)
  119. Re-culturing empty plasmid - (reply: 1)
  120. Ethidium precipitation in CsCl Maxiprep - (reply: 1)
  121. Plasmid Miniprep - (reply: 2)
  122. Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
  123. Plasmid DNA at RT for a few days - (reply: 4)
  124. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  125. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  126. plasmid rearrangement or sabotage - (reply: 2)
  127. Linearising plasmid before transfection? - (reply: 2)
  128. Smear in plasmid sample - (reply: 9)
  129. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  130. Accuracy of Nanodrop to measure plasmid concentration - (reply: 6)
  131. cloning a gene in to a plasmid - (reply: 5)
  132. Characterizing an unknown plasmid - (reply: 6)
  133. Plasmid Purification and Digestion Problem - (reply: 2)
  134. PDCD4 Plasmid, RAW 264.7 not working Help - (reply: 2)
  135. QuikChange with Different plasmids - (reply: 1)
  136. Co-transfection with 2 plasmids - (reply: 10)
  137. Single Plasmid Gel Band - (reply: 1)
  138. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  139. Transformation with a big plasmid - (reply: 4)
  140. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  141. Best ratio of 5 Plasmids for Lentivirus production - (reply: 4)
  142. digested plasmid - (reply: 2)
  143. Plasmid digested with RE used to transform...what will happen? - (reply: 14)
  144. isolation of plasmid (midi and maxi prep) - (reply: 18)
  145. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  146. Clean genomic DNA and plasmid DNA extraction from E.coli - (reply: 3)
  147. plasmid tetR and tet promoter - (reply: 2)
  148. Plasmid clone in BSP - (reply: 1)
  149. Swich selection in plasmid - (reply: 3)
  150. problems with midiprep - (reply: 3)
  151. Plasmid prep - no DNA pellet after ethanol precipitation - (reply: 4)
  152. Hypothetical Synthetic Plasmid - (reply: 9)
  153. How many fragments in plasmid DNA prep? - (reply: 1)
  154. Problem with plasmids transformation - (reply: 6)
  155. Calcium chloride plasmid transfection - (reply: 1)
  156. How Much Plasmid? - (reply: 6)
  157. Plasmid isolation - (reply: 1)
  158. plasmid;GI; ACESSION NUMBER - (reply: 2)
  159. virus transduction vs plasmid transfection - (reply: 4)
  160. Maxiprep of freeze/thawed pellet. - (reply: 1)
  161. Bizarre changes in plasmid band size - (reply: 3)
  162. Buffer composition for miniprep kit - (reply: 6)
  163. plasmid repository - (reply: 2)
  164. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  165. Falsely High Yields on Qiagen Miniprep? Assayed by Nanodrop - (reply: 3)
  166. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
  167. High molecular plasmid isolation form kit and alkaline lysis - (reply: 2)
  168. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  169. Need help verifying promotor of a PUC19 based plasmid - (reply: 1)
  170. Pls help me with this plasmid!!! - (reply: 1)
  171. Standard curve using plasmid - (reply: 1)
  172. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  173. Methods of plasmid transformation? only heat shock? - (reply: 1)
  174. No plasmid after a maxi prep mystery - (reply: 4)
  175. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  176. Co-expression of 2 plasmids in E.coli - (reply: 1)
  177. How to avoid plasmid instability - (reply: 4)
  178. Plasmid vs cDNA amplification by PCR - (reply: 2)
  179. Shear-induced damage of plasmid by vortexing - (reply: 1)
  180. RNase Band in old plasmid stored at -40 - (reply: 2)
  181. Plasmid vector for yeast and E. coli - (reply: 2)
  182. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  183. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  184. Plasmid digestion after transformation - (reply: 4)
  185. Puzzle about plamid and its digest, plz help - (reply: 8)
  186. Smear above plasmid dna - (reply: 3)
  187. result of plasmid extraction - (reply: 2)
  188. plasmid extraction - (reply: 5)
  189. Made the switch from Invitrogen Miniprep to Promega PureYield. What's going - (reply: 1)
  190. agarose gel electrophoresis of plasmid extraction - (reply: 12)
  191. plasmid extraction - (reply: 3)
  192. No DNA after midiprep - (reply: 2)
  193. Is that a Plasmid??? - (reply: 5)
  194. plasmid construction where I have to replace the chloramphenicol with kanamycin - (reply: 6)
  195. TRADITIONAL METHOD FOR PLASMIDEXTRACTION - (reply: 1)
  196. plasmid extraction - (reply: 13)
  197. Cloning two genes in the same plasmid - (reply: 4)
  198. why my solution II turns cloudy during plasmid extraction? - (reply: 6)
  199. Best plasmid for GFP + Mammalian Selection - (reply: 1)
  200. Plasmid, gene orientation, insert orientation. - (reply: 1)
  201. Plasmid recovery problem - (reply: 3)
  202. Electroporated plasmid loses the insert - (reply: 8)
  203. Plasmid sequencing without primers - (reply: 1)
  204. RE site on complimentary strand of plasmid? - (reply: 1)
  205. How much of plasmid should be cut to see the gene - (reply: 1)
  206. Plasmid sequencing - (reply: 1)
  207. Plasmid isolation Qiagen - (reply: 2)
  208. Is the T7 tag sequence immunogenic (from pET plasmid) - (reply: 2)
  209. Amplify fragment in plasmid? - (reply: 4)
  210. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  211. failure in miniprep - (reply: 1)
  212. Homemade MiniPreps for Plasmid Purification - (reply: 1)
  213. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  214. Template DNA (plasmid) concentration for PCR - (reply: 4)
  215. co-expression of 2 plasmids in E.coli - (reply: 3)
  216. How to choose the right plasmids for certain types of cell? - (reply: 1)
  217. Plasmid digestion create a bigger plasmid - (reply: 1)
  218. whole plasmid pcr? - (reply: 6)
  219. Looking for a GFP plasmid (non-amp) - (reply: 3)
  220. turbid DNA from maxiprep - (reply: 3)
  221. extract plasmids from transfected eukaryotic cells - (reply: 6)
  222. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  223. Plasmid compatible with pBAD24 - (reply: 1)
  224. Difference between plasmid DNA extraction and genomic DNA extraction - (reply: 3)
  225. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
  226. Endofree Maxiprep tube volume - (reply: 6)
  227. Miniprep OD beyond 2.00 - Troubleshooting-help desperately needed - (reply: 10)
  228. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
  229. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
  230. Low Miniprep Yield - (reply: 1)
  231. Site-Directed Mutagenesis Kit; any modified protocols for plasmids larger than 1 - (reply: 3)
  232. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  233. Problem with maxiprep - (reply: 2)
  234. ~200kb Plasmid DNA purification is not working - (reply: 1)
  235. ligating a 6 His tag to plasmid - (reply: 2)
  236. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
  237. Plasmid DNA size - Plasmid DNA backbone size (reply: 1)
  238. Plasmid sequencing failed why? - (reply: 10)
  239. Mini prep of frozen bacteria cells - (reply: 2)
  240. Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
  241. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  242. SLIM mutagenesis of large plasmids - (reply: 8)
  243. why 12 h culture for plasmid prep? - (reply: 2)
  244. Big plasmids and Transfection - (reply: 3)
  245. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
  246. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
  247. help: why plasmid yield dropped ever since the first culture - (reply: 2)
  248. pcr amplify an insert in plasmid? - (reply: 2)
  249. odd plasmids - (reply: 2)
  250. Lactobacillus plasmid - (reply: 1)
  251. Will transfected plasmids induce cytokine production? - (reply: 1)
  252. Question about plasmid midiprep - (reply: 1)
  253. Plasmid Miniprep yield problems - Low rate of yield using a Miniprep Kit (reply: 10)
  254. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  255. plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
  256. Overexpression plasmid is not expressing protein - (reply: 13)
  257. Extra band on gel after miniprep - (reply: 6)
  258. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
  259. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
  260. PCR problem - basic PCR for plasmid amplification (reply: 2)
  261. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
  262. Help Miniprep kit SIGMA - (reply: 2)
  263. why cut plasmid DNA with Hind111 and EcoR1? - (reply: 1)
  264. Low yields of plasmids from miniprep - (reply: 1)
  265. A gene is not inserted into the Plasmid - (reply: 2)
  266. Plasmid Question - (reply: 4)
  267. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
  268. Weird migration of double digested plasmid - (reply: 4)
  269. plasmid extraction and purification - methods to extract and purify plasmid to get high purity super coil (reply: 4)
  270. Help for co-transfection of proteins and plasmid - (reply: 1)
  271. Looking for a commercial bidirectional plasmid Help please! - resource hunting (reply: 2)
  272. How to store plasmid DNA on filter paper - I want to make a folder of all of them (reply: 3)
  273. Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
  274. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  275. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  276. plasmid DNA versus genomic DNA - concentrations (reply: 1)
  277. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  278. Undigestable/unrestrictable Plasmid? - (reply: 1)
  279. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  280. mini-prep plasmid isolation by alkaline lysis - genomic DNA vs plasmid DNA (reply: 6)
  281. Viruses and plasmid gaining resistance toward drugs can lead to speciatio - (reply: 3)
  282. problems encountered when working on a large plasmid - (reply: 2)
  283. make a Mega plasmid mutation - (reply: 1)
  284. plasmid cloning - i need help.. can anyone answer me (reply: 5)
  285. Plasmid standard problem - (reply: 2)
  286. Do plasmids work in bacteria? - (reply: 1)
  287. CEN sequences on plasmids (yeast) - (reply: 4)
  288. Plasmid out of BL21 for mutagensis - (reply: 3)
  289. ORI names - name of a plasmid ori (reply: 11)
  290. Alkaline Lysis Mini prep smeared on gel - (reply: 3)
  291. Change origin of a plasmid - (reply: 4)
  292. Better to sequence from plasmid? - (reply: 5)
  293. About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
  294. Does a single colony of E.coli contain more than one plasmid - (reply: 7)
  295. DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
  296. problem with pLK01 plasmid ligation - (reply: 2)
  297. Mini prep streaking - basic question (reply: 3)
  298. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  299. Maxiprep reagents - (reply: 5)
  300. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  301. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
  302. Can a single colony contains more than 2 types of insert in their plasmids? - (reply: 2)
  303. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
  304. PCR mutagenesis of plasmid - (reply: 5)
  305. Guanadine HCl for plasmid miniprep - (reply: 3)
  306. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
  307. Not getting plasmid from miniprep - (reply: 7)
  308. plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
  309. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
  310. Digesting large amount of plasmid - (reply: 4)
  311. Miniprep and sequencing - (reply: 4)
  312. Cloning <100 bp fragments into a plasmid - (reply: 7)
  313. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
  314. Help! my plasmid size changed after ligation - (reply: 5)
  315. Electroporation doesn't like my plasmid??? - (reply: 4)
  316. Cloning in pCR2.1 plasmid - (reply: 7)
  317. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  318. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
  319. Miniprep DNA-strange nanodrop peak at 240-250nm? - (reply: 4)
  320. Rnase A for plasmid miniprep - cheaper alternative to qiagen? (reply: 4)
  321. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  322. Cloning: Randomly recombined Plasmids? - (reply: 6)
  323. Stock not growing. Can i re-grow Plasmid from sample? - (reply: 15)
  324. plasmid + sepharose - how do this work? (reply: 1)
  325. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
  326. How to elute plasmid dried on paper? - I just received plasmid samples by mail (reply: 4)
  327. are vacuum manifolds (plasmid prep) interchange with brands? - (reply: 2)
  328. cloned plasmid expression less than original plasmid - after transfection (reply: 4)
  329. Plasmid size problems! - (reply: 3)
  330. Left DNA plasmid out for a week - (reply: 3)
  331. Transfecting primary cells with plasmid - (reply: 1)
  332. Extra bands after QIAGEN spin miniprep - (reply: 3)
  333. question about plasmid extraction - (reply: 2)
  334. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  335. Disappearing plasmid - (reply: 5)
  336. Transferring Plasmid from Aerobic to Anaerobic Bacteria - a thought about the oil eating microbes (OEMs) (reply: 1)
  337. Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
  338. problem in plasmid transfection - (reply: 2)
  339. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  340. Plasmid DNA mini-prep - no good isolation (reply: 5)
  341. Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
  342. iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
  343. plasmid extraction - why dilute (reply: 3)
  344. very high molecular weight plasmid - (reply: 6)
  345. purified dna using dh5alpha and qiaprep....plasmid not in genome - (reply: 3)
  346. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  347. Concepts behind plasmid prep - (reply: 2)
  348. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
  349. PCR off plasmid for screening - (reply: 2)
  350. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  351. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  352. Plasmid frameshift problem after clonning - (reply: 2)
  353. plasmid extraction kit wash buffer solution - (reply: 3)
  354. plasmid extraction kit solution composition - wash buffer (reply: 2)
  355. Miniprep yield for pET28 plasmid: 2.5ug. Too low? - Why such a low yield? (reply: 3)
  356. are plasmids used for lab work methylated - (reply: 1)
  357. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  358. why they use isopropanal for precipitate DNA in midiprep - (reply: 5)
  359. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  360. Isolation of protein crosslinked to plasmid - (reply: 3)
  361. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  362. Which Plasmid DNA for standard curve? - (reply: 5)
  363. Floating white pellet in miniprep - (reply: 2)
  364. Plasmid problem - From PCR product (reply: 7)
  365. Transfection - Plasmid Uptake Control - (reply: 2)
  366. Culture Grow well but No Plasmid - (reply: 2)
  367. software for plasmid drawing - (reply: 12)
  368. please urgently help: after phenol/chloroform extraction, my plasmid vector beca - (reply: 2)
  369. plasmid purification kit for transfection - (reply: 2)
  370. best plasmid purification kit for transfection - (reply: 1)
  371. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
  372. Checking Plasmid Expression - (reply: 1)
  373. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  374. PCR a plasmid protein - (reply: 2)
  375. plasmid DNA disappear in the minipre - (reply: 2)
  376. Interpreting a Plasmid Profile gel photo - (reply: 3)
  377. marker swap plasmid - (reply: 4)
  378. What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
  379. plasmid dna isolation - (reply: 1)
  380. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  381. siRNA and plasmid cotransfection - (reply: 1)
  382. rna from plasmid - (reply: 1)
  383. Miniprep - (reply: 3)
  384. miniPrep troubleshooting - (reply: 7)
  385. Mini prep - (reply: 4)
  386. Plasmid curing method - (reply: 4)
  387. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  388. plasmid DNA lab question - (reply: 11)
  389. Problem amplifying plasmid - (reply: 1)
  390. Doing mutagenesi for large plasmid or articificial chromosome - (reply: 1)
  391. Designing a Plasmid for p53 expression - I must choose between 3 given plasmids the best to express p53. (reply: 1)
  392. Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
  393. Help complementing my mutant in trans - help me find a plasmid (reply: 1)
  394. Transport of miniprep - (reply: 2)
  395. role of glucose in plasmid isolation? - (reply: 1)
  396. sequencing clones after BSP : need miniprep ?? - (reply: 1)
  397. Plasmids for protein expression in mammalian cells - (reply: 2)
  398. ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
  399. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  400. plasmid, BAC, or cosmid? - (reply: 3)
  401. Ligation of digested plasmid to hairpin oligo - (reply: 5)
  402. Nucleospin Extract II kit also for plasmids? - (reply: 5)
  403. Mutation during transformation/maxiprep - (reply: 1)
  404. Best plasmid miniprep? - (reply: 13)
  405. Plasmid instability in E.coli BL21? - (reply: 3)
  406. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  407. to construct a new plasmid(in E. coli), what features do i need - (reply: 1)
  408. Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
  409. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  410. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
  411. Plasmid DNA sequence and map - PLASMID DNA (reply: 2)
  412. Plasmid linearization by PCR - (reply: 4)
  413. plasmid sequence identification problem - (reply: 2)
  414. plasmid construct problem - (reply: 3)
  415. How to get plasmid maps from publications? - (reply: 5)
  416. pMSP12-GFP map? - How to get plasmid maps. (reply: 1)
  417. Best way to extract plasmid from Mycobacteria - Extraction of DNA (reply: 7)
  418. File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
  419. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  420. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  421. New Plasmid Draw/Plotter Program (Free) - (reply: 4)
  422. Plasmid DNA isolation - neutralisation of DNA (reply: 5)
  423. plasmid rescue (plasmids with incompatible ORI) - (reply: 2)
  424. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  425. Plasmid sequence - (reply: 1)
  426. How to exchange antibiotic resistance markers on a plasmid for E. coli - (reply: 2)
  427. plasmid isolation - (reply: 4)
  428. Plasmid supercoiling affecting PCR? - (reply: 2)
  429. Miniprep / plasmid question - How long can you get plasmid? (reply: 5)
  430. strange extra bands on digest of miniprep? - (reply: 5)
  431. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  432. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  433. problem with plasmid extraction - (reply: 3)
  434. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
  435. How to transfect two plasmids in a cell - (reply: 10)
  436. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
  437. How to separate large size plasmids ~12kb - (reply: 8)
  438. low plasmid yield from midi preps - (reply: 4)
  439. Plasmid isolation, help with dilution step? - (reply: 7)
  440. full digestion of plasmid to get only dNTPs - (reply: 3)
  441. size limit of plasmid origins - (reply: 2)
  442. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
  443. confusion with different forms of plasmid - (reply: 3)
  444. Plasmid as standard - Plasmid calculation (reply: 6)
  445. Toxic Plasmid?? Toxic Insert?? - (reply: 2)
  446. Effect of EtBr on plasmid supercoiling - (reply: 5)
  447. Quantifying plasmid DNA - Nanodrop and Qubit (Invitrogen) suck! (reply: 2)
  448. How to know the exact plasmid size? - - supercoiled, nicked, linear (reply: 6)
  449. Digestion of a plasmid - (reply: 1)
  450. Sequences of generally used plasmids? - Database for sequences available? (reply: 7)
  451. plasmid copy number - method for determination of plasmid copy number (reply: 2)
  452. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
  453. Amplifying plasmid - non specific binding of primer - (reply: 3)
  454. Storing plasmid at 4 degrees C - (reply: 5)
  455. Program to draw plasmids - draw plasmids (reply: 7)
  456. CIP required for double digestion of plasmid? - (reply: 3)
  457. Plasmid replication - Plasmid replication (reply: 13)
  458. Help with cloning a plasmid that contains repeats! - (reply: 2)
  459. Plasmid extraction sans commercial kits - (reply: 3)
  460. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
  461. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  462. mutations in plasmids - (reply: 3)
  463. Sequential restriction digestion of plasmid - (reply: 58)
  464. miniprep and maxiprep - (reply: 9)
  465. plasmid spin column usage - (reply: 5)
  466. Stored bacteria lost plasmids? - (reply: 12)
  467. difference between a linear and circular plasmid/DNA for stable transfections - will a circular plasmid stably integrate into the genome? (reply: 4)
  468. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  469. Selection during Extraction: Plasmid or Genomic DNA? - (reply: 1)
  470. PLASMID ISOLATION - (reply: 10)
  471. Frustrated with Plasmid Preps - need help! - (reply: 14)
  472. In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)